[PDF] Top 20 Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
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Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... characteristics. Screening appropriate reference genes is essential to reverse transcription quantitative real-time PCR (RT-qPCR), which is key to many ... See full document
11
Identification of Suitable Reference Genes for Investigating Gene Expression in Anterior Cruciate Ligament Injury by Using Reverse Transcription-Quantitative PCR.
... expression analysis may be a useful tool for under- standing ACL tears and healing ...failure. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) has emerged as an ... See full document
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Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.
... Quantitative real-time reverse transcription polymerase chain reaction (qPCR) is an efficient, specific, and reproducible method for quantifying transcript expression levels, and is ... See full document
10
Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
... role in studies aiming for the reliable examination of expression profiles gener- ated by high-throughput ...approaches. Real-time reverse transcription quantitative PCR ... See full document
11
RT-Q PCR ON R
... - Real time reverse transcription polymerase chain reaction (RT-qPCR) is an important technique to analyze differences in gene expression due to its sensitivity, accuracy, and ... See full document
9
Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
... microtubules in almost all eukaryotic cells, exhibited the most stable expression in the different ...normalized reference protein for Western blotting in Drosophila [25–27]. In this ... See full document
9
Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... with reference genes, whose expression does not change under different experimental ...Statistical analysis methods have been developed to identify the best reference genes for a ... See full document
8
Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).
... expression analysis plays a major role in understanding of the complex regulatory net- works and identification of genes relevant to new biological processes ...measurement, real time ... See full document
16
Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
... Quantitative real-time PCR (qRT-PCR) is now globally used for accurate analysis of tran- scripts levels in ...best reference genes is a prerequisite ... See full document
20
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as ... See full document
10
Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.
... blot analysis, ACTB, HPRT1 and GAPDH have been widely used as reference genes for qPCR analysis, despite the enormous body of evidence indicating that their transcription levels are not ... See full document
9
Validation of reference genes for real-time PCR of reproductive system in the black tiger shrimp.
... qPCR analysis, it seems to be a good internal control only for lowly expressed genes ...control in the gene expression studies of rice with environmental stresses [36] and the fathead minnow fish ... See full document
10
Quantitative analysis of the dystrophin gene by real-time PCR
... and reference exons were prepared over serially diluted genomic DNA sam- ...the PCR eiciencies of the target and the reference exons were approxi- mately equal, which was a prerequisite for the ac- ... See full document
6
BIOMEDICAL SCIENCES AND CLINICAL INVESTIGATION
... ing the PrimeScript RT-PCR kit (TaKaRa Bio Inc., Japan) according to manufacturer recommendations. The primers for the human PTPN12 gene were designed with Primer Premier 5.0 (Premier Biosoft, USA) and the ... See full document
8
Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions.
... expression in response to abiotic stress has been known to be regulated in ABA- dependent and/or ABA-independent manner [39,51–53]; thus, we also included ABA treatment into our ...candidate ... See full document
10
Application of time-series analysis for prediction of molding sand properties in production cycle
... The basic model used for residual data was simple linear mul- tivariate regression. However, for the 10 cases with largest pre- diction errors, also a regression tree model, based on the C&RT algorithm [8], was ... See full document
5
Acute exposure of apigenin induces hepatotoxicity in Swiss mice.
... especially in short term acute toxicity studies where the full phenotypic signs and symptoms may have not been fully developed ...[18]. In the present study, 48 differentially regulated genes were ... See full document
11
Genomic signatures predict poor outcome in undifferentiated pleomorphic sarcomas and leiomyosarcomas.
... candidate genes from this region had been studied in the context of ...expressed in all normal human tissues with increased expression in the ovary, lung, spleen, testis, and pancreas ... See full document
11
Real Time PCR and Importance of Housekeepings Genes for Normalization and Quantification of mRNA Expression in Different Tissues
... variations in its expression in the blood cells, being inappropriate for ...for real-time PCR analysis (Murphy et al. 2003). In the studies with lymphoid cells in ... See full document
12
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical Differential expression and functional characterization of cattle tick genes in response to pathogen infection (Babesia
... defective in the enzyme hypoxanthine guanidine phosphoribosyltransferase (HGPRT), they die when cultured in DMEM supplemented with HAT (aminopterin blocks the main DNA synthesis pathway and the alternative ... See full document
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