Top PDF Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR
... report of a systematic evalua- tion of potential reference genes with regard to their use- fulness as normalizers in malignant glioma expression ...keeping genes, we found that ...
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Importance of suitable reference gene selection for quantitative RT-PCR during ATDC5 cells chondrocyte differentiation.
... reference genes for in vitro chondrogenesis of ATDC5 cells, cells cultured in GM, IM and CM were analyzed at 1, 3, 5, 7 and 14 ...data of qPCR from different groups at various time ...
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Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria.
... group of photosynthetic prokaryotes, with a large morphological diversity including unicellular, colonial and filamentous ...specialized in photosynthesis (vegetative cells) and in nitrogen fixation ...
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Identification of reference genes for expression analysis by real‑time quantitative PCR in drought‑stressed soybean
... run in a 7300 RT-qPCR thermocycler (Applied Biosystems, Foster, CA, USA) using the following cycling parameters: 50°C for 2 min, 95°C for 10 min, 45 cycles at 95°C for 2 min, 62°C for 30 seconds and ...
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Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.
... reference genes, such as actin, a-tubulin, translation elongation factor, and ADP-ribosylation factor, belong to different ...families. Genes in the same family have conserved sequences. In ...
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Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).
... the expression variability of candidate ref- erence genes in ...vera using qRT-PCR. Our results demonstrated a wide variation of Ct values among the tested reference ...
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Identification of Suitable Reference Genes for Investigating Gene Expression in Anterior Cruciate Ligament Injury by Using Reverse Transcription-Quantitative PCR.
... one of the most frequently injured structures during high-impact sporting ...Gene expression analysis may be a useful tool for under- standing ACL tears and healing ...use of suitable ...
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Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
... real-time PCR (qRT-PCR) is now globally used for accurate analysis of tran- scripts levels in ...quantification of transcripts, identification of the best reference ...
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Evidence based selection of housekeeping genes.
... transcription PCR (RT-PCR) has become the method of choice due to its high sensitivity, specificity and broad quantification range for high- throughput and accurate expression profiling ...
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Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp
... transcription-quantitative PCR (RT-qPCR) is sensitive, specific and easy to operate, and is not only preferred in quantitative gene studies, but is also considered to be a “gold ...
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Selection of suitable housekeeping genes for real-time quantitative PCR in CD4(+) lymphocytes from asthmatics with or without depression.
... as in acute asthmatics or chronic asthmatics with continuous allergen exposure, PPIA expression would be higher than ...HKG in airway epithelial cells [26], this paper has provided helpful ...
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Inflammation Modulates RLIP76/RALBP1 Electrophile-Glutathione Conjugate Transporter and Housekeeping Genes in Human Blood-Brain Barrier Endothelial Cells.
... case of endothelial cells of the blood-brain barrier (BBB) of patients afflicted with neurodegenerative disorders such as Alzheimer's, Parkinson's, or multiple sclerosis, as well as in cases ...
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Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
... comparison of two different computer programs, geNorm [22] and NormFinder [23], was used to select the best internal controls for normalization of gene expression studies in ...comparison ...
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Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
... a suitable reference gene for expression normalization in ...involved in the process of protein translation, eIF-4a had the same performance as eEF-1a in most experimental ...
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Expression Stabilities of Candidate Reference Genes for RT-qPCR in Chinese Jujube (Ziziphus jujuba Mill.) under a Variety of Conditions.
... and analysis of gene function, an important part of any fol- low-up study, requires the appropriate selection of reference ...Indeed, suitable refer- ence gene selection ...
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A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression
... Statistical analysis. To identify genes for which expression levels were significantly different between FTA and FTC, we used the relative-expression data obtained from RT-PCR ...
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Downregulation of miR-205 modulates cell susceptibility to oxidative and endoplasmic reticulum stresses in renal tubular cells.
... result of densitmetry represent the means 6 S.E. of triplicate analysis from two independent ...(C) Quantitative real-time RT-PCR analysis revealed that the ...
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A versatile method to design stem-loop primer-based quantitative PCR assays for detecting small regulatory RNA molecules.
... generate quantitative data across large sample numbers ...generate quantitative data through several sample sets of newly identified or well known small RNAs, Northern-blot or quantitative ...
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Walking towards the “hidden” role of dirigent proteins in Vitis vinifera secondary metabolism
... characterization of the plant-pathogen interaction, ...Regardless of its economical impact, added knowledge on this interaction is of great significance, not only for viticulture, but also for a ...
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Identification and characterization of microRNAs from peanut (Arachis hypogaea L.) by high-throughput sequencing.
... energies of tRNA ...precursors of these miRNAs required 75–343 nt, with a majority of the identified miRNA precursors (88%) requiring 75–188 nt, similar to what had been observed in ...This ...
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