[PDF] Top 20 Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.

... GSC-like cells from human ESCs (Fig. 5). In particular, approximately 3% of the GSC-like cells could be differentiated into haploid male germ cells, exhibiting specific ... See full document

... over-activation of EGF signaling in vivo biases cortical progenitors to astrocyte lineage and reduces NSC self-renewal in the adult SVZ where as in vitro propagation of NSCs with EGF yields more glia ... See full document

... purpose of this study was to establish a method for monitoring the neural differentiation of stem cells using ferritin transgene expression, under the control of a ... See full document

... Spherical three-dimensional cell aggregates called embryoid bodies (EBs), have been widely used in in vitro differentiation protocols for human pluripotent stem cells including ... See full document

... the differentiation of monkey ES cells into germ cells in vitro, we produced EBs and examined the expression of germ cell markers using RT-PCR (Figure ... See full document

... expression of surface markers to differences in tissue sources, the method of isolation and culture, and species differences (Silva et ...Schäffler and Büchler, ...expression ... See full document

... level of pluripotency ...well of the PCR reactions l of SYBR Green, l of water, l of primers and l of ...sec and 60°C for 60 sec. Real-time PCR data and ... See full document

... identity of SSEA1+/cKit bright cells derived from ...iPGCs and cultured them on mouse embryonic fibroblasts (MEFs) supplemented with basic Fibroblast Growth Factor 2 (FGF2), Stem ... See full document

... contraction of hESC-derived SMPCs, cells were exposed to the CaM-specific inhibitor W-7 [32], and then contraction was induced by exposing them to the CaM agonist U46619 ...evaluation ... See full document

... iPS cells have been generated using a diverse range of tissues from a variety of donors using different reprogramming ...these cell lines are heterogeneous, which presents a limitation for ... See full document

... enucleation of the denuded MII oocytes was performed using a small piezo-driven ...staining and UV light ...fresh human cumulus cells and then activated using ionomycin and 6-di- ... See full document

... destruction of ß-cells is due to multiple genetic predisposi- tions and is also related to environmental factors that are still poorly defined ...80% of the ß-cell mass have been ... See full document

... use of ADSC from the inguinal region, decreasing skin lap necrosis, contribute to approximate the experimental use to clinical ...realization of immunohistochemical markers seeking whether there is an ... See full document

... characterization of both control and AS 30y iNs suggested that the infected fibroblasts subjected to neural direct conversion, or at least the great majority of them, did not acquired neuronal ... See full document

... Gene expression in human neural stem cells: effects of leukemia inhibitory factor // J.. Embryonic stem cells capable of differen- tiating into desired cell lines / J.[r] ... See full document

... irrespective of the type of treatment ...likelihood of cross-contamination with the use of urine separating (Ecosan) toilets ...part of the total nutrients in normal sewage; 80% ... See full document

... Expression patterns of cell-surface molecules on male germ line stem cells during postnatal mouse development.. Mol Reprod Dev.[r] ... See full document

... combinatorial cell intrinsic and neighboring effect that influenced the cells to undergo neural specification and differen- tiation from ES ...initial cell plating density thus ... See full document

... Pluripotent human embryonic stem cells (hESCs) have the capacity to differentiate into all of the somatic cell types and therefore hold great promise for regenerative ... See full document

... multiple embryonic development pertur- bations in presence of potential toxicants, it is critical to determine the optimal time point of hESC ...days embryonic development related genes ... See full document