Top PDF Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
... GSC-like cells from human ESCs (Fig. 5). In particular, approximately 3% of the GSC-like cells could be differentiated into haploid male germ cells, exhibiting specific ...
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Physiological normoxia and absence of EGF is required for the long-term propagation of anterior neural precursors from human pluripotent cells.
... over-activation of EGF signaling in vivo biases cortical progenitors to astrocyte lineage and reduces NSC self-renewal in the adult SVZ where as in vitro propagation of NSCs with EGF yields more glia ...
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Use of Ferritin Expression, Regulated by Neural Cell-Specific Promoters in Human Adipose Tissue-Derived Mesenchymal Stem Cells, to Monitor Differentiation with Magnetic Resonance Imaging In Vitro.
... purpose of this study was to establish a method for monitoring the neural differentiation of stem cells using ferritin transgene expression, under the control of a ...
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Physical passaging of embryoid bodies generated from human pluripotent stem cells.
... Spherical three-dimensional cell aggregates called embryoid bodies (EBs), have been widely used in in vitro differentiation protocols for human pluripotent stem cells including ...
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In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.
... the differentiation of monkey ES cells into germ cells in vitro, we produced EBs and examined the expression of germ cell markers using RT-PCR (Figure ...
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The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells
... expression of surface markers to differences in tissue sources, the method of isolation and culture, and species differences (Silva et ...Schäffler and Büchler, ...expression ...
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Male germ-like cell differentiation potential of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells in co-culture with human placenta cells in presence of BMP4 and retinoic acid
... level of pluripotency ...well of the PCR reactions l of SYBR Green, l of water, l of primers and l of ...sec and 60°C for 60 sec. Real-time PCR data and ...
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Single cell analysis facilitates staging of Blimp1-dependent primordial germ cells derived from mouse embryonic stem cells.
... identity of SSEA1+/cKit bright cells derived from ...iPGCs and cultured them on mouse embryonic fibroblasts (MEFs) supplemented with basic Fibroblast Growth Factor 2 (FGF2), Stem ...
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Towards the maturation and characterization of smooth muscle cells derived from human embryonic stem cells.
... contraction of hESC-derived SMPCs, cells were exposed to the CaM-specific inhibitor W-7 [32], and then contraction was induced by exposing them to the CaM agonist U46619 ...evaluation ...
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Genetic background drives transcriptional variation in human induced pluripotent stem cells.
... iPS cells have been generated using a diverse range of tissues from a variety of donors using different reprogramming ...these cell lines are heterogeneous, which presents a limitation for ...
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Reprogramming of Human Somatic Cells Using Human and Animal Oocytes
... enucleation of the denuded MII oocytes was performed using a small piezo-driven ...staining and UV light ...fresh human cumulus cells and then activated using ionomycin and 6-di- ...
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Secondary prevention of type 1 diabetes mellitus: stopping immune destruction and promoting ß-cell regeneration
... destruction of ß-cells is due to multiple genetic predisposi- tions and is also related to environmental factors that are still poorly defined ...80% of the ß-cell mass have been ...
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Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats
... use of ADSC from the inguinal region, decreasing skin lap necrosis, contribute to approximate the experimental use to clinical ...realization of immunohistochemical markers seeking whether there is an ...
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Exploring cell reprogramming techniques for Angelman Syndrome disease modelling
... characterization of both control and AS 30y iNs suggested that the infected fibroblasts subjected to neural direct conversion, or at least the great majority of them, did not acquired neuronal ...
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Expression and secretion of human recombinant LIF by genetically modified mammalian cells
... Gene expression in human neural stem cells: effects of leukemia inhibitory factor // J.. Embryonic stem cells capable of differen- tiating into desired cell lines / J.[r] ...
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Assessment Of NPK In Human Male And Female Urine For Its Fertilising Potential In Agriculture
... irrespective of the type of treatment ...likelihood of cross-contamination with the use of urine separating (Ecosan) toilets ...part of the total nutrients in normal sewage; 80% ...
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Biologia e nicho de espermatogônias tronco em catetos (tayassu tajacu) adultos
... Expression patterns of cell-surface molecules on male germ line stem cells during postnatal mouse development.. Mol Reprod Dev.[r] ...
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Neural induction from ES cells portrays default commitment but instructive maturation.
... combinatorial cell intrinsic and neighboring effect that influenced the cells to undergo neural specification and differen- tiation from ES ...initial cell plating density thus ...
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Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.
... Pluripotent human embryonic stem cells (hESCs) have the capacity to differentiate into all of the somatic cell types and therefore hold great promise for regenerative ...
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Identification of thalidomide-specific transcriptomics and proteomics signatures during differentiation of human embryonic stem cells.
... multiple embryonic development pertur- bations in presence of potential toxicants, it is critical to determine the optimal time point of hESC ...days embryonic development related genes ...
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