[PDF] Top 20 Stepwise development of hematopoietic stem cells from embryonic stem cells.

... promoter from Cytomegalovirus (CMV); the rabbit beta-globin 2nd intron; human HOXB4 cDNA; an internal ribosome entry site (ires); EGFP cDNA; and a polyA ...Stabl2 cells (Invitrogen), purified using a ... See full document

... establishment of apico-basal polarity and development of proper stereocilliary bundles is likely to occur in induced ...supporting cells can transmit polarity information to HCs. Supporting ... See full document

... regeneration of mammary gland. Mammary stem cells are maintained within specific microenvironments (niches) in the mammary gland and these mammary stem cell niches can be reconstituted de novo ... See full document

... pluripotent stem cells (iPSCs) obtained by the ectopic expression of defined transcription factors have tre- mendous promise and therapeutic potential for regenerative ...and embryonic ... See full document

... Identiication of hemangioblast functionality As previously mentioned, cells from early- stage aggregates (14-day) were cultured in con- ditions known to support the growth of blast ... See full document

... Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have the ability to proliferate indefinitely in an undifferentiated state, and to differentiate to ... See full document

... treatment of trauma, burns, tumor resection, congenital malforma- tions and other causes of soft tissue defects are ...increased development of adipose tissue engineering, new ideas have been ... See full document

... accumulation of clonal plasma cells in bone marrow. This causes a build-up of monoclonal im- munoglobulin in blood and/or urine, and the presence of a peak in the gamma zone of serum ... See full document

... gin-expressing cells during the early stage of co-culture differentiation (Fig ...CHA15 cells were transferred onto γ-irradiated MEF-noggin feeder cells for 7–10 days before placing them on a ... See full document

... Techniques of separation, purification as well as cryopreservation of marrow, blood, cord blood or tissue cells have been developed in blood ...radically from total blood transfusion to cell ... See full document

... existence of loss and gain of chromosomes, known as aneuploidy, has been previously described within the central nervous ...During development, at least one-third of neural progenitor ... See full document

... results from a cell-mediated autoimmune attack against pancreatic ...identification of ß-cell precursors have been made not only with the aim of understanding the physiology of islet ... See full document

... comprised of two copies of each of the core histones (H2A, H2B, H3, and H4) wrapped by 147 base pairs (bp) of DNA, with the symmetrical center being called the ...location of ... See full document

... majority of the informants accepted the legal use of human embryos in research, but this did not mean that most of their projects used this ...adult stem cells more productive and less ... See full document

... assessment of the hematopoietic toxicity and leukemogenicity of benzene and its metabolites during mouse embryonic ...effects of benzene metabolites were much pronounced in ... See full document

... is of immense interest in biomedical research, especially for stem cell-based therapies and fertility ...cryopreservation of cells and tissues, and a combination of dimethyl sulfoxide ... See full document

... muscle cells in the laboratory and then transplant those cells into patients with chronic heart disease ...form of stem cell therapy that is widely ... See full document

... population of NSCs, rosettes were manually isolated using stretched glass Pasteur pipette and placed in fresh culture ...single cells using accutase and replated onto culture dishes to obtain a homogeneous ... See full document

... 23 of 96 patients treated for malignant dis- ...29.31% of the patients in the BM group and ...incidences of relapse were 45% in the BM group and 25% in the UCB group at two years (Figure ...stage ... See full document

... range of sources, including epigenetic memory [5–10,17], inherent differences between IPSCs and ESCs [4,28], artifacts of reprogramming [7] or lab environment ...effects of genetic background have ... See full document