Top PDF Stepwise development of hematopoietic stem cells from embryonic stem cells.
Stepwise development of hematopoietic stem cells from embryonic stem cells.
... promoter from Cytomegalovirus (CMV); the rabbit beta-globin 2nd intron; human HOXB4 cDNA; an internal ribosome entry site (ires); EGFP cDNA; and a polyA ...Stabl2 cells (Invitrogen), purified using a ...
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From embryonic stem cells to sensory hair cells : a programming approach
... establishment of apico-basal polarity and development of proper stereocilliary bundles is likely to occur in induced ...supporting cells can transmit polarity information to HCs. Supporting ...
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Reconstitution of mammary epithelial morphogenesis by murine embryonic stem cells undergoing hematopoietic stem cell differentiation.
... regeneration of mammary gland. Mammary stem cells are maintained within specific microenvironments (niches) in the mammary gland and these mammary stem cell niches can be reconstituted de novo ...
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The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method
... pluripotent stem cells (iPSCs) obtained by the ectopic expression of defined transcription factors have tre- mendous promise and therapeutic potential for regenerative ...and embryonic ...
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Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells
... Identiication of hemangioblast functionality As previously mentioned, cells from early- stage aggregates (14-day) were cultured in con- ditions known to support the growth of blast ...
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A defined, feeder-free, serum-free system to generate in vitro hematopoietic progenitors and differentiated blood cells from hESCs and hiPSCs.
... Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have the ability to proliferate indefinitely in an undifferentiated state, and to differentiate to ...
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Xinghui Song1 , Yanwei Li1 , Xiao Chen2
... treatment of trauma, burns, tumor resection, congenital malforma- tions and other causes of soft tissue defects are ...increased development of adipose tissue engineering, new ideas have been ...
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Stromal cells in the hematopoietic-stem-cell niche
... accumulation of clonal plasma cells in bone marrow. This causes a build-up of monoclonal im- munoglobulin in blood and/or urine, and the presence of a peak in the gamma zone of serum ...
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Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.
... gin-expressing cells during the early stage of co-culture differentiation (Fig ...CHA15 cells were transferred onto γ-irradiated MEF-noggin feeder cells for 7–10 days before placing them on a ...
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Rev. Bras. Hematol. Hemoter. vol.22 número3
... Techniques of separation, purification as well as cryopreservation of marrow, blood, cord blood or tissue cells have been developed in blood ...radically from total blood transfusion to cell ...
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Retinoic acid-treated pluripotent stem cells undergoing neurogenesis present increased aneuploidy and micronuclei formation.
... existence of loss and gain of chromosomes, known as aneuploidy, has been previously described within the central nervous ...During development, at least one-third of neural progenitor ...
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Secondary prevention of type 1 diabetes mellitus: stopping immune destruction and promoting ß-cell regeneration
... results from a cell-mediated autoimmune attack against pancreatic ...identification of ß-cell precursors have been made not only with the aim of understanding the physiology of islet ...
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Nucleosome Organization in Human Embryonic Stem Cells.
... comprised of two copies of each of the core histones (H2A, H2B, H3, and H4) wrapped by 147 base pairs (bp) of DNA, with the symmetrical center being called the ...location of ...
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Interface (Botucatu) vol.11 número23 en a13v1123
... majority of the informants accepted the legal use of human embryos in research, but this did not mean that most of their projects used this ...adult stem cells more productive and less ...
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Regulation of PKM2 and Nrf2-ARE pathway during benzoquinone induced oxidative stress in yolk sac hematopoietic stem cells.
... assessment of the hematopoietic toxicity and leukemogenicity of benzene and its metabolites during mouse embryonic ...effects of benzene metabolites were much pronounced in ...
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Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media.
... is of immense interest in biomedical research, especially for stem cell-based therapies and fertility ...cryopreservation of cells and tissues, and a combination of dimethyl sulfoxide ...
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Stem cells – Current concepts and Future applications
... muscle cells in the laboratory and then transplant those cells into patients with chronic heart disease ...form of stem cell therapy that is widely ...
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Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.
... population of NSCs, rosettes were manually isolated using stretched glass Pasteur pipette and placed in fresh culture ...single cells using accutase and replated onto culture dishes to obtain a homogeneous ...
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Rev. Bras. Hematol. Hemoter. vol.37 número4
... 23 of 96 patients treated for malignant dis- ...29.31% of the patients in the BM group and ...incidences of relapse were 45% in the BM group and 25% in the UCB group at two years (Figure ...stage ...
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Genetic background drives transcriptional variation in human induced pluripotent stem cells.
... range of sources, including epigenetic memory [5–10,17], inherent differences between IPSCs and ESCs [4,28], artifacts of reprogramming [7] or lab environment ...effects of genetic background have ...
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