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[PDF] Top 20 Targeted amino-terminal acetylation of recombinant proteins in E. coli.

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Targeted amino-terminal acetylation of recombinant proteins in E. coli.

Targeted amino-terminal acetylation of recombinant proteins in E. coli.

... products of PCR reactions where appropriate primers had been Figure ...Generation of an ...expression of the NatB complex and target proteins in ... See full document

5

Genes involved in cell division in mycoplasmas

Genes involved in cell division in mycoplasmas

... mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a ... See full document

8

A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide

A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide

... and in contrast to pRSETA. While pET3- His and pAE permit the expression of a polypeptide with a minimal number of 9 amino acids (including the hexa-histidine tag) fused at the N-terminus (for ... See full document

7

Induced Immune Response of Escherichia coli BL21 Expressing Recombinant MSP1a and MSP1b Proteins of Anaplasma marginale

Induced Immune Response of Escherichia coli BL21 Expressing Recombinant MSP1a and MSP1b Proteins of Anaplasma marginale

... gene in all ...variation in MSP1b1 and MSP1b2 sequences during the multiplication phases in cattle and tick cells (Oberle et ...composed of a highly conserved C-terminal region and an ... See full document

8

Produção da enzima antileugêmica L-Asparaginase II a partir da clonagem do Gene ErA de Erwinia Carotovora Supsp. Atroseptica em Escherichia Coli

Produção da enzima antileugêmica L-Asparaginase II a partir da clonagem do Gene ErA de Erwinia Carotovora Supsp. Atroseptica em Escherichia Coli

... N-terminal amino acid sequencing showed the removal of the N-terminal signal peptide from ...the recombinant L- asparaginase protein in one compartment and avoid leakage to ... See full document

53

A model for the RecA protein of Mycoplasma synoviae

A model for the RecA protein of Mycoplasma synoviae

... similarity of RecA proteins from mycoplasmas, E. coli and ...E. coli (Gammaproteo- bacteria class) (Doolittle, 1999), the amino acid sequence identity is higher between RecA ... See full document

6

Collagen-like proteins in pathogenic E. coli strains.

Collagen-like proteins in pathogenic E. coli strains.

... sequences of enterohaemorrhagic E. coli O157:H7 strains show multiple open-reading frames with collagen- like sequences that are absent from the common laboratory strain ...included in prophages ... See full document

21

Towards a functional understanding of protein N-terminal acetylation.

Towards a functional understanding of protein N-terminal acetylation.

... the proteins Forte et al. found retained in the cytosol when acetylated all depended on ...properly targeted to the Sec61 translocon, meaning that the acetyl group most likely disrupts the ... See full document

5

Determination Of Longevity Of Teeth In Buckets Of Loading Equipment In Coal Mines - A Case Study

Determination Of Longevity Of Teeth In Buckets Of Loading Equipment In Coal Mines - A Case Study

... effects of machine scale, wear surface structure of the rolls, grinding pressures and rolls speed, gap settings, feed size distribution and moisture content for a range of ...wear of the ... See full document

11

Cloning, expression and characterization of a cold-adapted endo-1, 4-β-glucanase from Citrobacter farmeri A1, a symbiotic bacterium of Reticulitermes labralis

Cloning, expression and characterization of a cold-adapted endo-1, 4-β-glucanase from Citrobacter farmeri A1, a symbiotic bacterium of Reticulitermes labralis

... mass of the recombinant endoglu- canase was approximately 42 ...activity of the E. coli pET22b-EglC crude ex- tract was ...pH of 7.0. The recombinant enzyme had an optimal ... See full document

13

RINL, guanine nucleotide exchange factor Rab5-subfamily, is involved in the EphA8-degradation pathway with odin.

RINL, guanine nucleotide exchange factor Rab5-subfamily, is involved in the EphA8-degradation pathway with odin.

... radioactivity of GTP and GDP was quantified, and the percentages (%) of each GTP-bound Rab are ...percentages of each GTP-bound Rab member in the metabolically radiolabeled cells are shown as ... See full document

9

Immunogenicity of Recombinant Proteins Consisting of Plasmodium vivax Circumsporozoite Protein Allelic Variant-Derived Epitopes Fused with Salmonella enterica Serovar Typhimurium Flagellin

Immunogenicity of Recombinant Proteins Consisting of Plasmodium vivax Circumsporozoite Protein Allelic Variant-Derived Epitopes Fused with Salmonella enterica Serovar Typhimurium Flagellin

... (CSP)-based recombinant fusion vaccine is the first malaria vaccine to reach phase III clinical ...production of antibodies to the immunodominant central repeat region of the ...CSP. In ... See full document

8

Compositional Changes of Proteins and Amino Acids in Germinating Coffee Seeds

Compositional Changes of Proteins and Amino Acids in Germinating Coffee Seeds

... tissue in coffee seeds. In mature seeds a small embryo is situated near one end of the curved side of the ...inside of the seed, occupying the space released by the inner part of ... See full document

7

Production of Recombinant Proteins in Milk of Transgenic and Non-transgenic Goats

Production of Recombinant Proteins in Milk of Transgenic and Non-transgenic Goats

... produce recombinant proteins from years to weeks (Bösze et ...efficiency of different promotors, removing the action of variables associated with the effect of the position or number ... See full document

12

A double built-in containment strategy for production of recombinant proteins in transgenic rice.

A double built-in containment strategy for production of recombinant proteins in transgenic rice.

... one of the most important food crops worldwide, the first and foremost concern for the utilization of pharmaceutical transgenic rice is the environmental biosecurity and food ...possibility of ... See full document

16

Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.

Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.

... production of the proteins whose expression was decreased in GCDM-grown cells, we initially determined how expression was affected by inducer concentration and induction ...expression of the ... See full document

12

Iliano V Coutinho-Abreu, Marcelo Ramalho-Ortigao

Iliano V Coutinho-Abreu, Marcelo Ramalho-Ortigao

... role in male gamete fertility (van Dijk et ...protein in its native form using different systems, such as bac- teria (Milek et ...expression of a truncated form of Pfs48/45 (Pfs48/45-10C) ... See full document

12

Identification of two tyrosine residues required for the intramolecular mechanism implicated in GIT1 activation.

Identification of two tyrosine residues required for the intramolecular mechanism implicated in GIT1 activation.

... structure of GIT1 allows the interaction with several partners. Binding of GIT1 to some of its partners requires activation of the GIT1 ...binding of paxillin to GIT1 is enhanced by ... See full document

12

Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper

Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper

... activity of fractions and purified enzyme was assessed on citrated human plasma and on a 4 mg/mL solution of bovine fibrinogen, dissolved in PBS, as described ...Samples of 100 µL fractions or ... See full document

6

A C2H2 zinc finger protein FEMU2 is required for fox1 expression in Chlamydomonas reinhardtii.

A C2H2 zinc finger protein FEMU2 is required for fox1 expression in Chlamydomonas reinhardtii.

... series of cellular ...consists of iron reductase (FRE1), a protein involved in iron assimilation (FEA1), and a transport complex comprising a multi-copper oxidase (FOX1) and an Fe transporter (FTR1), ... See full document

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