O controle da expressão de Let-7b-5p é mediado via ativação do eixo PPARβ/LIN28a. Neste contexto, o aumento na transativação do PPARβ parece estar diretamente associado a indução de LIN28a e, consequentemente, redução na expressão do let-7b-5p. Além disso, nossos resultados mostram que o PGC1α é alvo direto do Let-7b-5p, sugerindo que o controle da expressão desse miRNA contribui com a manutenção do processo de biogênese e função mitocondrial em células musculares esqueléticas. Vale ressaltar que o controle desse eixo envolvendo, fator de transcrição, co-reguladores e miRNA, abre perspectivas para o controle de doenças metabólicas associadas ao processo de disfunção mitocondrial em tecidos periféricos.
Figura 16. Sumário Gráfico. A ativação de PPARβ pelo exercício físico, PGC1α ou por GW501516 é responsável pela aumento na expressão de LIN28a, uma proteína ligante de mRNA, conhecida também com proteína esponja, que impede o processo de maturação do miRNA Let-7b-5p. Por outro lado, bloqueio de PPARβ por antagonista GSK0660 ou siRNA, reduz a expressão de Lin28a e aumenta a expressão de Let-7b-5p.
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