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O tempo e a temperatura não influenciaram significativamente (p > 0,05) o comportamento microbiano tanto na maturação úmida quanto a seco. A condução dos processos de maturação a seco e em special bag a 85% UR resultou em altas contagens de micro-organismos aeróbios mesófilos e psicrotróficos, acarretando em deterioração. O método de descongelamento não influenciou nas contagens microbianas do produto após maturação (p > 0,05). De forma geral, a condução do processo de maturação a seco a 2 ou 7 ºC a 75% UR ou a 2 ºC a 65% UR por até 42 dias resultou em contagens ≤ 5,00 log UFC/g.

5. REFERÊNCIAS BIBLIOGRÁFICAS

AHNSTRÖM, M. L. et al. Dry aging of beef in a bag highly permeable to water vapour. Meat Science, v. 73, p. 674–679, 2006.

BERGER, J. et al. Dry-aging improves meat quality attributes of grass-fed beef loins. Meat Science, v. 145, n. January, p. 285–291, 2018.

BOGDANOWICZ, J.; CIERACH, M.; ŻMIJEWSKI, T. Effects of aging treatment and freezing/thawing methods on the quality attributes of beef from Limousin × Holstein- Friesian and Hereford × Holstein-Friesian crossbreeds. Meat Science, v. 137, p. 71– 76, 2018.

CAMPBELL, R. E. et al. Dry-aging effects on palatability of beef longissimus muscle. Journal of Food Science, v. 66, n. 2, p. 196–199, 2001.

CERVENY, J.; MEYER, J. D.; HALL, P. A. Microbiological Spoilage of Meat and Poultry Products. In: DOYLE, M. P.; SPERBER, W. H. (Eds.). Compendium of Microbiological Spoilage of Food and Beverages. 1. ed. New York: Springer, 2009. p. 69–86.

CHOE, J.-H.; STUART, A.; KIM, Y. H. B. Effect of different aging temperatures prior to freezing on meat quality attributes of frozen/thawed lamb loins. Meat Science, v. 116, p. 158–164, 2016.

DASHDORJ, D. et al. Dry aging of beef ; Review. Journal of Animal Science and Technology, p. 1–11, 2016.

DEGEER, S. L. et al. Effects of dry aging of bone-in and boneless strip loins using two aging processes for two aging times. Meat Science, v. 83, n. 4, p. 768–774, 2009. EFSA – European Food Safety Authority. Regulamento nº 2073/2005 da comissão de 15 de novembro de 2005 relativo a critérios microbiológicos aplicáveis aos gêneros alimentícios. Disponível em: <https://bit.ly/2EauOtv>. Acesso em: 09 jun. 2018.

FUNG, D. Y. C. Yeasts and Molds. Encyclopedia of Meat Science, p. 835-846, 2014. GILL, C. O. Spoilage, factors affecting: Microbiological. Encyclopedia of Meat Science, p. 388-393, 2014.

GUDJÓNSDÓTTIR, M. et al. Effects of electrospun chitosan wrapping for dry-ageing of beef , as studied by microbiological , physicochemical and low-field nuclear magnetic resonance analysis. Food Chemistry, v. 184, p. 167–175, 2015.

HULÁNKOVÁ, R. et al. The effect of dry aging on instrumental, chemical and microbiological parameters of organic beef loin muscle. LWT - Food Science and Technology, v. 89, p. 559–565, 2018.

IAL – Instituto Adolfo Lutz. Métodos Físico-químicos para análise de alimentos: normas analíticas do Instituto Adolfo Lutz. 4 ed., Brasília: ANVISA, 2005.

ICMSF – International Comission on Microbiological Specifications for Foods. Meat and Meat Products. In: Microorganisms in Foods 6: Microbial Ecology of Food Commodities. 2th ed. Springer, New York, p. 1-106. 2005.

KIANI, H.; SUN, D. W. Water crystallization and its importance to freezing of foods: A review. Trends in Food Science & Technology and Technology, v. 22, n. 8, p. 407– 426, 2011.

KIM, Y. H. B. et al. Effects of stepwise dry/wet-aging and freezing on meat quality of beef loins. Meat Science, v. 123, p. 57–63, 2017.

KIM, Y. H. B. et al. Evaluation of combined effects of ageing period and freezing rate on quality attributes of beef loins. Meat science, v. 110, p. 40–45, 2015.

KIM, Y. H. B.; KEMP, R.; SAMUELSSON, L. M. Effects of dry-aging on meat quality attributes and metabolite profiles of beef loins. Meat Science, v. 111, p. 168–176, 2016.

KOOHMARAIE, M.; GEESINK, G. H. Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system. Meat Science, v. 74, p. 34–43, 2006.

KORNACKI, J. L.; GURTLER, J. B.; STAWICK, B. A. Enterobacteriaceae, Coliforms, and Escherichia coli as Quality and Safety Indicators. In: SALFINGER, Y.; TORTORELLO, M. L. (Eds.). Compendium of Methods for the Microbiological Examination of Foods. 5th. ed. Washington, D.C: Americam Public Health Association, 2015. p. 103–120.

quality of meat: Review. Meat Science, v. 91, n. 2, p. 93–98, 2012.

LI, X. et al. A comparative study of beef quality after ageing longissimus muscle using a dry ageing bag, traditional dry ageing or vacuum package ageing. Meat Science, v. 97, n. 4, p. 433–442, 2014.

LI, X. et al. Meat quality, microbiological status and consumer preference of beef gluteus medius aged in a dry ageing bag or vacuum. Meat Science, v. 95, p. 229–234, 2013.

MATARNEH, S. K. et al. The Conversion of Muscle to Meat. In: TOLDRÁ, F. (Ed.). Lawrie´s Meat Science. 8. ed. Cambridge: Woodhead, 2017. p. 159–185.

NJONGMETA, N. A. et al. Acid-Producing Microorganisms. In: SALFINGER, Y.; TORTORELLO, M. L. (Eds.). Compendium of Methods for the Microbiological Examination of Foods. 5th. ed. Washington, D.C: Americam Public Health Association, 2015. p. 229–236.

PENNACCHIA, C.; ERCOLINI, D.; VILLANI, F. Spoilage-related microbiota associated with chilled beef stored in air or vacuum pack. Food Microbiology, v. 28, n. 1, p. 84– 93, 2011.

PITT, J. I.; HOCKING, A. D. Fungi and Food Spoilage. 3. ed. New York: Springer, 2009.

POTHAKOS, V. et al. Lactic acid bacteria and their controversial role in fresh meat spoilage. Meat Science, v. 109, p. 66–74, 2015.

PrimeSafe - Agency of the Government of the State of Victoria, Australia. Aging of Beef. Disponível em: <https://goo.gl/ZR5imZ>. Acesso em: 03 jun. 2017.

RYSER, E. T.; SCHUMAN, J. D. Mesophilic Aerobic Plate Count. In: SALFINGER, Y.; TORTORELLO, M. LOU (Eds.). Compendium of Methods for the Microbiological Examination of Foods. 5th. ed. Washington, D.C: Americam Public Health Association, 2015. p. 96–101.

RYU, D.; WOLF-HALL, C. Yeasts and Molds. In: SALFINGER, Y.; TORTORELLO, M. L. (Eds.). Compendium of Methods for the Microbiological Examination of Foods. 5th. ed. Washington, D.C: Americam Public Health Association, 2015. p. 277–286.

RYU, S. et al. Diversity and characteristics of meat microbiological community on dry aged beef. Journal of microbiology and biotechnology, v. 28, n. 1, p. 105–108, 2018.

SÄDE, E.; MURROS, A.; BJÖRKROTH, J. Predominant enterobacteria on modified- atmosphere packaged meat and poultry. Food Microbiology, v. 34, n. 2, p. 252–258, 2013.

STENSTRÖM, H. et al. Consumer preference and effect of correct or misleading information after ageing beef longissimus muscle using vacuum, dry ageing, or a dry ageing bag. Meat Science, v. 96, n. 1, p. 661–666, 2014.

TAP, W. N. Investigation of mycologic growth, aflatoxin production, and human neural processing associated with quality differences in dry-aged meat products. 2016. Dissertation (Animal and Food Science) Texas Tech University, Texas, 2016.

VASAVADA, P. C.; CRITZER, F. J. Psychrotrophic Microorganisms. In: SALFINGER, Y.; TORTORELLO, M. L. (Eds.). Compendium of Methods for the Microbiological Examination of Foods. 5th. ed. Washington, D.C: Americam Public Health Association, 2015. p. 175–189.

ZAGOREC, M.; CHAMPOMIER-VERGÈS, M.-C. Meat Microbiology and Spoilage. In: TOLDRÁ, F. (Ed.). Lawrie´s Meat Science. 8. ed. Cambridge: Woodhead, 2017. p. 187–200.

CAPITULO IIl

EFFECT OF DIFFERENT DRY AGING TEMPERATURES ON LISTERIA INNOCUA AS SURROGATE FOR LISTERIA MONOCYTOGENES

Astrid Caroline Muniz da Silva, Pâmela de Oliveira Pena, Sérgio Bertelli Pflanzer Júnior e Maristela da Silva do Nascimento.

Abstract

The objective of this study was to evaluate the effect of different dry aging temperatures on the behavior of Listeria innocua used as a surrogate of L. monocytogenes. The process was performed in boneless loin pieces for up to 42 days at 2 and 8°C. The behavior of L. innocua was influenced by the temperature, aging time, and the water activity of the beef surface (P < 0.05). After 42 days, reductions of 2.38 and 3.37 log cfu/g were obtained on the beef surface aged at 2 and 8°C, respectively. According to data predicted by the Weibull model, the samples aged at 2°C would achieve a 4-log reduction with twice the time required for the process at 8°C. After trimming, 66.7% of the samples aged at 2°C were positive for L. innocua, whereas at 8°C the rate was 33.3%. Therefore, the results showed that the increase of process time and temperature as well by decrease of the aw reduces L. innocua counts.

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