Os resultados obtidos a partir do método estabelecido para a produção dos sistema nanoestruturado apresentaram reprodutibilidade satisfatória. A natureza lipofílica, o tamanho dos lipossomas, potencial zeta, índice de polidispersão e formato do lipossoma obtidos são indicativos para avanços na utilização destas formulações como veículos de drug delivery para estratégias passivas e ativas de seletividade, podendo aumentar a concentração intracelular de drogas no interior de células-alvo e, ao mesmo tempo, evitar a toxicidade para células sadias, diminuindo assim os efeitos colaterais sistêmicos das drogas utilizadas atualmente no tratamento do câncer, ao passo que, aumenta a qualidade de vida do paciente durante o tratamento. Ademais, os resultados obtidos apresentam-se bastante promissores.
O conjunto de dados in silico, resultados prévios e da presente dissertação permitiram o desenvolvimento de um nanossistema carreador de N-ciclohexil-1,2,4-oxadiazol. O resultado in vitro obtido do composto encapsulado quando comparado com a droga livre indica um efeito de inibição de crescimento relacionado apenas à droga, uma vez que o lipossoma controle não apresentou atividade, denotando que os compostos apresentam grande potencial farmacêutico. Para isso, mais testes físico-químicos, ensaios in vitro e ensaios in vivo devem ser realizados para que seja possível avaliar a manutenção da atividade antitumoral com redução da toxicidade desses compostos.
ANEXOS
ARTIGO ACEITO PARA PUBLICAÇÃO
Artigo: Nanoparticles with Antitumoral Activity: An Integrative Literature Review
Autores: José Geraldo de Alencar Santos Júnior, João Pedro Viana Rodrigues, Vanessa Pinheiro Gonçalves Ferreira and Roberto Nicolete.
Revista: Journal of Nanomedicine & Nanotechnology ISSN: 2157-7439
Abstract: Nanotechnology is used to describe materials, devices and systems with structures and components, exhibiting new and/or significantly improving physical, chemical and biological properties. In the biomedical area, these materials have unique nanoscale properties of 1 to 100 nanometers. Thus, the objective was to evidence the available scientific knowledge in the literature on some advances related to the use of nanoparticles with antitumor activity. The present study uses as an integrative literature review method, allowing to search, evaluate and synthesize the available evidence to contribute to the development of the discussion about the theme. Crossing the descriptors "nanoparticles" and "antitumoral", 62 studies were found, of which one was repeated in more than one place. Thus, of the 61 publications listed, 26 addressed the main theme proposed with emphasis on the use of nanoparticles/nanoemulsion against tumor cells, in this way were selected to compose this study. Nanotechnology can be considered a new technology in the oncology area. The major challenges are to gather information about the properties and risks of these nanomaterials, the in vivo application as well as specific clinical trials. DOI: 10.35248/2157-7439.19.10.544
ARTIGO SUBMETIDO
Artigo: Liposomal nanoparticles containing synthetic drug derivates: development and characterization
Autores: João Pedro Viana Rodrigues, Vanessa Pinheiro Gonçalves Ferreira, José Geraldo dos Santos Alencar, Marlos Chaves e Roberto Nicolete.
Revista: Applied Biotechnology and Biochemistry 1559-0291
Abstract: Modified drug delivery systems are pharmaceutical preparations with strong appeal avant-garde in the frontier of knowledge. The preparation of the liposomes used dipalmitoyl phosphatidylcholine, dipalmitoyl phosphatidylserine and cholesterol. The components were dissolved in a chloroform/methanol solution. The solvents were evaporated, and it was kept in a desiccator. After resuspending it was formed an emulsion. The emulsion was subjected to the extruder to produce a similar liposome population. The determination of the particle size, particle size distribution and zeta potential were performed. For the quantification of the material encapsulated, liquid chromatography coupled to mass spectrometry (UPLC-QToF- MSE). The characterization showed monodisperse populations of small-sized particles of 200 nm for control and encapsulated liposomes and showing a good polydispersity index for both. Liposomes remained with an average size for encapsulated liposomes. The zeta potential that was represented the residual negative charge on the surface of the particles. The results obtained in atomic force and scanning microscopy corroborated to attest to the size of the liposome and allowed to verify its spherical shape. The consistency of results obtained from the established method endorse its effectiveness and efficiency.
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