O presente estudo mostrou que o peptídeo CAADIVGQCPAKLK induziu a produção e acúmulo de espécies reativas de oxigênio, que levaram a morte celular programada (apoptose) em células de Candida albicans e Sacaromices cerevisiae. A morfologia das células de Candida e Saccharomyces sugere que a superfície celular estava intacta, evidenciando que a inibição ocorreu por meio de interação com receptor não ocasionando danos à parede celular e membrana plasmática.
Os ensaios utilizando Iodeto de Propídio, SEM, DCFD-A, TUNEL e ensaios de docking molecular sugerem que PEP4 ocasiona a morte celular programada em S. cerevisiae e C. albicans ao se ligar ao receptor PHO36. Os resultados indicam que osmotinas e taumatinas, assim como peptídeos derivados estudados neste trabalho, são candidatos futuros para o desenvolvimento de ferramentas biotecnológicas contra Candida albicans. O uso de bancos de dados como o C-PamP é essencial para a predição de novos peptídeos antimicrobianos. Portanto, apesar de existir uma ampla disponibilidade de várias classes de medicamentos antifúngicos no mercado mundial, o tratamento de pacientes com infecções fúngicas ainda é um desafio considerável.
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APÊNDICE A- FONTES FINANCIADORAS
O presente trabalho foi realizado com o suporte das seguintes instituições:
Universidade Federal do Ceará, por meio dos Laboratório de Biotecnologia de Proteases Vegetais, coordenado pelo professor Dr. Cleverson Diniz Teixeira de Freitas, Laboratório de Plantas Laticíferas do Departamento de Bioquímica e Biologia Molecular, coordenado pelo professor Dr. Márcio Viana Ramos, Laboratório de Toxinas e Proteínas Tóxicas de Plantas, coordenado pela professora Dra. Iika Maria Vasconselos, Labortórios de Proteínas de Defesas de Plantas, coordenado pelo professor Dr. José Tadeu Abreu de Oliveira.
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), por meio do fornecimento da bolsa de estudo.