A partir dos resultados e discussões anteriores, pode-se concluir que:
Padronizou-se uma reação de transcrição-reversa/ reação em cadeia da polimerase hemi-nested para detecção de coronavírus bovino (BCoV) em amostras fecais de bezerros e de bovinos adultos, tendo como alvo o gene codificador da proteína de nucleocapsídeo (N), que quando comparada com a nested RT-PCR dirigida ao gene RdPd apresentou uma concordância substancial.
Padronizou-se uma reação de transcrição-reversa/ reação em cadeia da polimerase hemi-nested para detecção de rotavírus do grupo A em amostras fecais de bezerros e de bovinos adultos, tendo como alvo o gene codificador da VP1 (RNA- polimerase RNA-dependente), que quando comparada com a PAGE apresentou uma concordância fraca.
Padronizou-se uma reação de transcrição-reversa/ reação em cadeia da polimerase hemi-nested (multiplex hemi-nested RT-PCR) para detecção simultânea de coronavírus bovino (BCoV) e rotavírus em amostras fecais de bezerros, a partir das reações mencionadas anteriormente, que quando comparada a nested RT-PCR dirigida ao gene RdPd e a PAGE concordância ótima e substancial, respectivamente; e quando comparada com as reações individuais para BCoV e RV, apresentou concordância substancial.
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