A análise dos resultados permite concluir que:
O pré-tratamento com a ouabaína (0,56 mg/kg), mostrou uma ação anti- inflamatória uma vez que foi capaz de reduzir a migração de células para o sítio da inflamação;
A ouabaína reduz a subpopulação de linfócitos T CD3+ no fluido do lavado broncoalveolar (BALF);
A ouabaína atenuou a produção de citocinas características do fenótipo Th2, IL-4 e IL-13, porém não interferiu nos níveis de IFN- ;
As alterações histopatológicas do modelo de inflamação pulmonar alérgica foram moduladas negativamente pela oubaína;
A ouabaína reduziu a proporção do título de IgE-OVA específica presente no BALF.
Figura 12. Esquema representando os efeitos da ouabaína no processo inflamatório alérgico pulmonar obtidos neste trabalho
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APÊNDICE A - Substâncias e sais Ácido acétido glacial (C2H4O2) – VETEC Ácido clorídrico (HCl) –VETEC
Ácido fosfotunguístico (H3PW12O40) – VETEC Ácido periódico (HIO4) – VETEC
Álcool metílico (CH3OH) – VETEC Aldeído fórmico (CH2O) – MERCK
Alúmen de potássio (KAI(SO4)2) – MERCK Anticorpo Monoclonal: anti-CD3
Bissulfito de sódio (NaHSO3) – MERCK Carvão ativado – VETEC
Cloral hidratado – VETEC
Cloreto de potássio (KCl) – MERCK Cloreto de sódio (NaCl) – SIGMA Cloridrato de quetamina – VETBRANDS Clorofórmio (CHCl3) – LTF
Corante azul de evans – VETEC Corante cromotropo 2R – VETEC Corante eosina Y – VETEC Corante hematoxilina – VETEC Corante Panóptico – NEWPROV Corante test-green F.C.F. – VETEC Corante violeta de genciana – VETEC
Dexametasona – Aché ®
D-gluxose (C6H12O6) – SIGMA Etanol (C2H5OH) – CBiotec Ficoeritrina
Fosfato de potássio (KH2PO4) – REAGEN Fosfato de sódio anidro (Na2HPO4) – MERCK Formaldeído – MERCK
Glicerina (C3H3(OH)3) – VETEC
Hidróxido de alumínio (Al(OH)3) – VETEC Hidróxido de sódio (NaOH) – REAGEN Metabissulfito de sódio (Na2S2O5) – MERCK Ouabaína – SIGMA
OVA grade II – SIGMA OVA grade V – SIGMA
Óxido de mercúrio amarelo (Hg2O) – LAFAN Parafina para Histologia – COAL
Timol (C10H14O) – VETEC Xilol (C6H4(CH3)2) – VETEC
APÊNDICE B - Aparelhos e equipamentos
Agitador Vortex – VWR – Scientific Pro ducts Autoinclusor – Leica EG 120
Balança analítica – Sartorius Banho histológico – ANCAP
Centrífuga refrigerada - Centra MP4R – International Equipement Company (IEC)
Citômetro – FACS Callibur Estufa – ICAMO – modelo 3
Histotécnico processador automático de tecidos – OMA – DM – 40 Microscópio óptico – Nikon
Micrótomo – Leica RM 2125 – RT
ANEXOS
ANEXO B – Artigo em colaboração publicado em 2015 (Mediators of Inflammation - WebQualis CAPES: B2; Fator de impacto: 3,236; Online ISSN: 0962-9351).
ANEXO C - Artigo em colaboração publicado em 2015 (frontiers in Physiology - WebQualis CAPES: B1; Fator de impacto: 3,534; Online ISSN: 1664-042X).
ANEXO D – Artigo submetido com os resultados desta dissertação (Immunobiology - WebQualis CAPES: B1; Fator de impacto: 3,044; Online ISSN: 0171-2985).