Considerando os resultados obtidos nesta pesquisa foi possível detectar a presença de bactérias dos gêneros Streptococcus e Enterococcus em águas captadas de galerias pluviais e nas águas marinhas influenciadas pela descarga destas.
Pode-se determinar mediante a quantificação de bactérias dos gêneros Streptococcus e Enterococcus nas águas avaliadas, o aporte da carga bacteriana das galerias ao fluxo de entrada e contaminação das águas marinhas da orla de Fortaleza.
A utilização do caldo Todd-Hewitt não mostrou eficiência no favorecimento do crescimento das bactérias alvo (Streptococcus e Enterococcus).
Um percentual alto (96,79%) de isolados apresentou resistência a um ou mais antibióticos testados o que destaca o risco maior por exposição a agentes potencialmente patogênicos.
A alta multirresistência detectada nas culturas isoladas mostrou percentuais alarmantes frente a antimicrobianos de uso comum na terapêutica clínica, o que justifica a importância da vigilância e controle deste grupo de microrganismos no ambiente.
Pode-se verificar, mediante a cura plasmidial, que a resistência apresentada pelos isolados, indicativa de transferência plasmidial, ratifica a capacidade dessas bactérias em adquirir e disseminar determinantes de resistência antibiótica.
Os gêneros Streptococcus e Enterococcus devem ser reconhecidos por sua potencial contribuição à dispersão da resistência nos ambientes aquáticos (pluviais e marinhos), fato de interesse e importância para a saúde pública.
Diversidade genética alta dos isolados multirresistentes Streptococcus e Enterococcus analisados foi observada mediante a utilização de Box-PCR indicando a necessidade de uma abordagem polifásica na identificação de espécies em estirpes ambientais.
REFERÊNCIAS
ABDELSALAM, M.; EISSA, A.E.; CHEN, S.-C. Genetic diversity of
geographically distinct Streptococcus dysgalactiae isolates from fish. Journal of Advanced Research. Amsterdam, v.6, p. 233-238, 2015.
ABRIOUEL, H.; OMAR, N.B.; LÓPEZ, R.L.; GÁLVEZ, A. La doble faceta del género Enterococcus y su importancia en alimentos. Anales de la Real Academia de Ciencias Veterinarias. Andalucía, v.21, n.1, p. 65-83, 2008. ABRIOUEL, H.; OMAR, N.B.; MOLINOS, A.C.; LÓPEZ, R.L.; GRANDE, M.J.; MARTÍNEZ-VIEDMAN, P.; ORTEGA, E.; CAÑAMERO, M.M.; GALVEZ, A. Comparative analysis of genetic diversity and incidence of virulence factors and antibiotic resistance among enterococcal populations from raw fruit and
vegetable foods, water and soil, and clinical samples. International Journal of Food Microbiology. Amsterdam,v. 123, p. 38-49, 2008.
ABUALTAYEF, M.T.; FATTAH, A.; RABOU, A.; ABUFOUL, A.A.; GHABAYEN, S.M.; ELSINWAR, H.M. Microbial water quality of coastal recreational water in the Gaza Strip, Palestine. Nusantara Bioscience. Kehati, v. 6, n.1, p. 26-32, 2014.
AHMAD, A.; DADA, A.Ch.; USUP, G. Survival of Epidemic, Clinical, Faecal and Recreational Beach Enterococci Strains with Putative Virulence Genes in
Marine and Fresh Waters. Journal of Environmental Protection. Georgetown, v. 5, p. 482-492, 2014
ALBUFERA, U.; BHUGALOO-VIAL, P.; ISSACK, M. I.; JAUFEERALLY-FAKIM, Y. Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis. Infection, Genetics and Evolution. Amsterdam, v. 9, p. 322-327, 2009.
ALCAIDE, F.; BENÍTEZ, M.A.; CARRATALÁ, J.;GUDIOL, F.; LIÑARES, J.;MARTÍNS, R. In Vitro Activities of the New Ketolide HMR 3647
(Telithromycin) in Comparison with Those of Eight Other Antibiotics against Viridans Group Streptococci Isolated from Blood of Neutropenic Patients with Cancer. Antimicrobial Agents of Chemotherapy. Washington, v. 45, n.2, p. 624–626, 2001
ALIPOUR, M.; HAJIESMAILI, R.; TALEBJANNAT, M.; YAHYAPOUR, Y. Identification and Antimicrobial Resistance of Enterococcus Spp. Isolated from the River and Coastal Waters in Northern Iran. The Scientific World Journal. Cairo, v.2014, p. 1-5, 2014.
ALM, E.W.; BURKE, J. SPAIN, A. Fecal indicator bacteria are abundant in wet sand at freshwater beaches. Water Research. Amsterdam, v. 37, p. 3978- 3982, 2003.
ALONSO, M.; MARIMON, J.M.; ERCIBENGOA, M.; PÉREZ-YARZA, E.G.; PÉREZ-TRALLERO. Dynamics of Streptococcus pneumoniae Serotypes
Causing Acute Otitis Media Isolated from Children with Spontaneous Middle- Ear Drainage over a 12-Year Period (1999–2010) in a Region of Northern Spain. PLoSONE. Califórnia, v.8, issue 1, e54333, 2013.
ÁLVAREZ, M.D.; IZQUIERDO, C.C.S.; PAZ, M.T.F.; IZQUIERDO, A.M. Características clínicas y epidemiológicas de las infecciones por enterococos en el niño. 2007
Disponible em: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0034- 75312007000100008&lng=e . Acesso em: 12 DEZ. 2014.
ÁLVAREZ, M.D.; PAZ, M.T.F.; ARIAS, R.I.A.; IZQUIERDO, A.M.; IZQUIERDO, C.S. Infecciones por enterococos en neonatos egresados de la Maternidad en Cuba. Revista Panamericana de Infectología. OPAS-WHO, v.10, n.3, p. 14- 20, 2008.
AMBROSE; K.D.; NISBET, R.; STEPHENS, D.S. Macrolide Efflux in
Streptococcus pneumoniae Is Mediated by a Dual Efflux Pump (mel and mef) and Is Erythromycin Inducible. Antimicrobial Agents and Chemotherapy. Washington, v. 49, n.10, p. 4203–4209, 2005.
ANDERSSON, D.I.; HUGHES, D. Antibiotic resistance and its cost: is it possible to reverse resistance. Nature Reviews Microbiology. London, v. 8, p. 260- 271, 2010.
ANDERSSON, A.; LARSSON, U.; HAGSTROM, A. Size selective grazing by a microflagellate on pelagic bacteria. Marine Ecology Progress Series.
Oldendorf/Luhe-Germany, v.33, p. 51–57, 1986.
ANDERSON, K.L.; WHITLOCK, J.E.; HARWOOD, V.J. Persistence and Differential Survival of Fecal Indicator Bacteria in Subtropical Waters and Sediments. Applied and Environmental Microbiology. Washington, v. 71, n.6, p. 3041-3048, 2005.
ANDERSON, R.; FELDMAN, C. Key virulence factors of Streptococcus pneumoniae and non-typeable Haemophilus influenzae: roles in host defence and immunisation. Southern African Journal Epidemiology Infections. Joanesburgo, v.26, n.1, p. 6-12, 2011.
APPELBAUM, P.C. Resistance among Streptococcus pneumoniae: Implications for drug selection. Clinical Infectious Diseases. Oxford, v. 34, p. 1613–1620, 2002.
APHA-AWWA-WPCF. American Public Health Assn; American Water Works Assn., and Water Pollution Control Federation. Standard Methods for the
Examination of Water and Waste Water; 18 th Edition; section 9230; Wahington D.C., 1992.
ARDANUY, C.; DOMENECH, A.; ROLO, D.; CALATAYUD, L.; TUBAU, F.; AYATS, J.; MARTÍN, R.; LIÑARES, J. Molecular characterization of macrolide- and multidrug-resistant Streptococcus pyogenes isolated from adult patients in
Barcelona, Spain (1993–2008). Journal of Antimicrobial Chemotheraphy. Oxford, v.65, p. 634-643, 2010.
ARIAS, C.A.; MURRAY, B.E. The rise of the Enterococcus: beyond vancomycin resistance. Nature Reviews Microbiology. London, v.10,n. 4, p. 266-278, 2012.
ARIAS, C.A.; CONTRERAS, G.A.; MURRAY, B.E. Management of multidrug- resistant enterococcal infections.Clinical Microbiology and Infections. Amsterdan, v. 16, n.6, p. 555-562, 2010.
ASHBOLT, N.J.; GRABOW, W.O.K.; SNOZZI, M. Indicators of microbial water quality. In: Water Quality: Guidelines, Standards and Health; Fewtrell, L.; Barthram, J., Eds; IWA Publishing: London, UK, p. 289-316, 2001.
AYER, V.; TEWODROS, W.; MANOHARAN, A.; SKARIAH,S.; LUO, F.;
BESSEN, D.E. Tetracycline resistance in group a streptococci: emergence on a global scale and influence on multiple-drug resistance Antimicrobial Agents of Chemotherapy. Washington, v. 51, n.5, p. 1865–1868, 2007.
BACHOON, D.S.; MARKAND, S.; OTERO, E.; PERRY, G.; RAMSUBAUGH, A. Assessment of non-point sources of fecal pollution in coastal waters of Puerto Rico and Trinidad. Marine Pollution Bulletin. Amsterdam, v. 60, p. 1117-1121, 2010.
BALDINI, M; SELZER, P. Patrones de resistencia a antibióticos de enterococos aislados de aguas estuarinas. Revista Argentina de Microbiología. Buenos Aires, v. 40, p. 48-51, 2008.
BARBOSA, T.; LEVI, S. The impact of antibiotic use on resistance development and persistence. Drug Resistance Updates. Amsterdam, v. 3, p. 303-311, 2000.
BARCINA, I.; LEBARON, P.; VIVES-REGO, J. Survival of allochthonous bacteria in aquatic systems: A biological approach. FEMS Microbiology and Ecology. Oxford, v. 23, p. 1-9, 1997.
BAUER, A. W.; KIRBY, M. M.; SHERRIN, J. D. Atibiotcs susceptibility testing by standardized single disk method. American Journal of Pathology.
Amsterdam, v.45, p.493-496, 1966.
BENDER, E.A; FREITAS, A.L.P; REITER, K.C; LUTZ, L; BARTH, A.L. Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. Isolated in Porto Alegre, Brazil. Brazilian Journal Microbiology. São Paulo, v.40, p. 693-700, 2009.
BENTO, V.R.S. Centro e periferia em Fortaleza sob a ótica das
disparidades na infraestrutura de saneamento básico. 173f. Dissertação (Mestrado Acadêmica em Geografia, do Centro de Ciências e Tecnologia) Universidade Estadual de Ceará, Fortaleza, 2011.
BERNARDO, M.S.M.V. Comparação dos métodos aplicados na detecção de bactérias coliformes, Escherichia coli e Enterococcus sp. em águas para fins recreativos.121f. Dissertacao (Mestrado em
Qualidade em
Análises. Especialização em Análises de Águas). Faculdade de Ciências
e Tecnologia. Universidade de Algarve, Faro, 2007.
BINGEN, E.; BIDET, P.; MIHAILA-AMROUCHE, L.; DOIT, C.; FORCET, S.; BRAHIMI, N.; BOUVET, A.; COHEN, R. Emergence of Macrolide-Resistant Streptococcus pyogenes Strains in French Children. Antimicrobial Agents and Chemotherapy. Washington, v. 48, n. 9, p. 3559–3562, 2004.
BITTON, G. Microbial indicators of fecal contamination application to microbial source tracking. Department of Environmental Engineering Sciences. University of Florida. Report submitted to the Florida Stormwater Association.
Gainesville/FL, 71 p., 2005.
BOEHM, A.B.; SASSOUBRE, L.M. Enterococci as indicators of environmental fecal contamination.2014, In: Gilmore MS, Clewell DB, Ike Y, et al., editors. Enterococci: From Commensals to Leading Causes of Drug Resistant Infection [Internet]. Boston: Massachusetts Eye and Ear Infirmary; California, 17p., Fevereiro 2014-. Disponível em:
http://www.ncbi.nlm.nih.gov/books/NBK190421/. Acesso em: 18 FEV. 2015. BOEHM, A.B. Enterococci concentrations in diverse coastal environments exhibit extreme variability. Environmental Sciences and Technology. Washington, v. 41, n. 24, p. 8227–8232, 2007.
BOEHM, A.B.; WEIBERG, S.B. Tidal forcing of enterococci at marine recreational beaches at fortnightly and semi-diurnal frequencies.
Environmental Sciences and Technology. Washington, v.39, n.15, p. 5575– 5583, 2005.
BONILLA, N.; SANTIAGO, T.; MARCOS, P.; URDANETA, M.; SANTO
DOMINGO, J.; TORANZOS, G.A. Enterophages, a group of phages infecting Enterococcus faecalis, and their potential as alternate indicators of human faecal contamination. Water Science and Technology. Amsterdam, v.61, n.2, p. 293–300, 2010.
BORDEAUX, M.; FLORES, A.;GAINA, R.; ONG, D.; PATEL, N. WONG, C. Fecal indicator bacteria concentrations after rainfall events: Evaluating the 72- hour rain advisory period. In: UCLA Institute of the Environment and
Sustainability. Environmental Science Senior Practicum. Califórnia, 17 p., 2012.
BOSCH-MESTRES, J; MARTÍN-FERNÁNDEZ, R.M; ANTA-LOSADA, M.T.J. Estudio comparativo de tres medios de cultivo para detectar la colonización por estreptococo del grupo B en la mujer embarazada. Enfermedades Infecciosas y Microbiología Clínica. Amsterdam, v. 21, n.7, p. 346-349, 2003.
BOSSHARD, P.P.; ABELS, S.; ALTWEGG, M.; BÖTTGER, E.C.; ZBINDEN, R. Comparison of Conventional and Molecular Methods for Identification of Aerobic Catalase-Negative Gram-Positive Cocci in the Clinical Laboratory. Journal of Clinical Microbiology. Washington, v.42, n.5, p. 2065–2073, 2004.
BRAGA, M.P.; PIOVESAN, A.; VALARINI, N.; MACIEL, S.M.; ANDRADE, F. B.; POLI-FREDERICO, R.C. Genotypic diversity and virulence factors of
Streptococcus mutans in caries-free and caries-active individuals. Brazilian Archives of Biology and Technology. Paraná, v.56, n. 2, p. 241-248, 2013. BRASIL. Resolução CONAMA No. 274, de 29 de novembro de 2000, Diário Oficial da República Federativa do Brasil, Brasilia, 2000.
BRENCIANI, A.; TIBERI, E.; TILI, E.; MINGOIA, M.; PALMIERI, C.; VARALDO, P.E.; GIOVANETTI, E. Genetic determinants and elements associated with antibiotic resistance in viridans group streptococci. Journal of Antimicrobial Chemotherapy. Oxford, v.69, p. 1197–1204, 2014.
BRENCIANI, A.; TIBERI, E.; MORCI, E.; ORYANS, E.; GIOVANETTI, E.; VARALDO, P.E. ICESp1116, the genetic element responsible for erm(B)- mediated, inducible resistance to erythromycin in Streptococcus pyogenes. Antimicrobial Agents Chemotherapy. Washington, v. 56, n.12, p. 6425–6429, 2012.
BRENCIANI, A.; TIBERI, E.; BACCIAGLIA, A.; PETRELLI, D.; VARALDO, P.E.; GIOVANETTI, E. Two distinct genetic elements are responsible for erm(TR)- mediated erythromycin resistance in tetracycline-susceptible and
tetracycline-resistant strains of Streptococcus pyogenes. Antimicrobial Agents Chemotherapy. Washington, v. 55, n.5, p. 2106–2112, 2011.
BRENCIANI, A. ; BACCIAGLIA, A.; VIGNAROLI, C.; PUGNALONI, A.;
VARA D , P.E. GI VANE I, E. Фm46.1, the main Streptococcus pyogenes element carrying mef(A) and tet(O) genes. Antimicrobial Agents
Chemotherapy. Washington, v. 54, n.1, p. 221–229, 2010.
BRENCIANI, A. ; BACCIAGLIA, A.; VECHI, M.; VITALI, L.A.; VARALDO, P.E.; GIOVANETTI, E. Genetic Elements Carrying erm(B) in Streptococcus pyogenes and Association with tet(M) Tetracycline Resistance Gene. Antimicrobial
Agents Chemotherapy. Washington, v. 51, n.4, p. 1209–1206, 2007. BRITO, D.A.; RAMIREZ, M.; LENCASTRE, H. Serotyping Streptococcus
pneumoniae by Multiplex PCR. Journal of Clinical Microbiology. Washington, v. 41, n. 6, p. 2378–2384, 2003.
BRYSKIER, A. Viridans group streptococci: a reservoir of resistant bacteria in oral cavities. Clinical Microbiology and Infection. Amsterdam, v. 8, n.2, p. 65-69, 2002.
BUGANO, D.D.; CAVALCANTI, A.B.;GONCALVES, A.R.; ALMEIDA, C.S.; SILVA, E. Cochrane meta-analysis: teicoplanin versus vancomycin for
proven or suspected infection. Einstein, São Paulo, v. 9,n. 3, p. 265-282, 2011. BUCHANAN, J.T.; COLVIN, K.M.; VICKNAIR, M.R.; PATEL, S.K.; TIMMER, A.M.; NIZET, V. Strain-associated virulence factors of Streptococcus iniae in hybrid-striped bass. Veterinary Microbiology. Amsterdam, v. 13, p. 145- 153, 2008.
BYAPPANAHALLI, M.N; NEVERS, M.B; KORAJKIC, A; STALEY, Z.R; HARWOOD, V.J. Enterococci in the Environment. Microbiology and Molecular Biology Reviews. Washington, v. 76, n.4, p. 685–706, 2012. BYAPPANAHALLI, M.N; WHITMAN, R.L; SHIVELY, D.A; EVERT, W .T; TING, W.T.E.; TSENG, C.C; NEVERS, M.B. Seasonal persistence and population characteristics of Escherichia coli and enterococci in deep backshore sand of two freshwater beaches. Journal of Water and Health. London, v. 4, n.3, p. 313-320, 2006.
BYAPPANAHALLI, M.N.; SHIVELY, D.A.; NEVERS, M.B.; SADOWSKY, M.J.; WHITMAN, R.L. Growth and survival of Escherichia coli and enterococci opulations in the macro-alga Cladophora (Chlorophyta). FEMS Microbiology Ecology. Amsterdam, v. 46, p. 203-211, 2003.
CAI, Y.; KONG, F.; GILBERT, G.L. Three new macrolide efflux (mef) gene variants in Streptococcus agalactiae. Journal of Clinical Microbiology. Washington, v. 45, p. 2754–2755, 2007.
CAMILA, K. Esgoto na Beira-Mar alerta para poluição da orla. Diário do Nordeste Online, Fortaleza, 8 jul. 2010. Caderno Cidade. Disponível em: < http://diariodonordeste.globo.com/materia.asp?codigo=811184>. Acesso em: 18 FEV. 2015.
CAMILLI, R.; GROSSO, M.; IANNELLI, F.; PANTOSTI, A. New genetic element carrying the erythromycin resistance. Antimicrobial Agents and
Chemotherapy. Washington, v.52, n.2, p. 619-625, 2008.
CARR, M.R.; WANG, T.I.; McLEAN, T.I.; FLOOD, C.J.; ELLENDER, R.D. Salmonella rarely detected in Mississippi coastal waters and sediment. Journal of Applied Microbiology. Oxford, v. 109, p. 2191–2199, 2010.
CARVALHO, E., M., R.; COSTA, A., R.; ARAÚJO, A., J., G.; SOUSA, O., V.; VIEIRA, R., H., S., F. Multiple antibiotic-resistance of Enterococcus insolated from coastal water near an outfall in Brazil. African Journal of Microbiology. Oxford, v. 8, n. 17, p. 1825-1831, 2014.
CARVALHO, M.G.S. ; SHEWMAKER, P.L.; STEIGERWALT, A.G.; MOREY, R.E.; SAMPSON, A.J.; JOYCE, K.; BARRETT, T.J.; TEIXEIRA, L.M.;
FACKLAM, R.R. Enterococcus caccae sp. nov., isolated from
human stools. International Journal of Systematic and Evolutionary Microbiology. Cambridge, v.56, p. 1505-1508, 2006.
CASTILLO-ROJAS, G.; MAZARI-HIRÍARTE, M.; LEÓN, S.P.; AMIEVA- FERNÁNDEZ, R.I.; AGIS-JUÁREZ, R.A.; HUEBNER, J.; LÓPEZ-VIDAL, Y. Comparison of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Water and Clinical Samples: Antimicrobial Susceptibility and Genetic Relationships. PLoS ONE. Califórnia, v. 8, issue 4, e59491, 2013. CETESB - Companhia Ambiental do Estado de São Paulo. Enterococos – Determinação pela técnica de membrana filtrante: método de ensaio. Norma técnica L5.212. Versão Junho/2012. Segunda edição. Diário Oficial do Estado de São Paulo, Brasil, 19 páginas, 2012.
CHAMBERS, H.F. Penicillin-binding protein-mediated resistance in
pneumococci and staphylococci. Journal of Infectious Diseases. Oxford, v.179, (Suppl 2), p. S353-359, 1999.
CHAMPAGNE, J.; DIARRA, M.S.; REMPEL, H.; TOPP, E.; GREER, W.; HAREL, J.; MASSON, L. Development of a DNA Microarray for Enterococcal species, virulence, and antibiotic resistance gene determinations among
isolates from poultry. Applied and Environmental Microbiology. Washington, v. 77, n.8, p. 2625-2633, 2011.
CHIU, C-H.; SU, L-H.; HUANG, Y-C.; LAI, J-C.; CHEN, H-L.;WU, T-L.; LIN, T-Y. Increasing Ceftriaxone Resistance and Multiple Alterations of Penicillin-Binding Proteins among Penicillin-Resistant Streptococcus pneumoniae Isolates in Taiwan. Antimicrobial Agents and Chemotherapy. Washington, v. 51, n.9, p. 3404–3406, 2007.
CHO, J.W. Aminoglycoside Resistance in Enterococci. Clinical Infectious Diseases. Oxford, v.31, p. 586-590, 2000.
CLAVERYS, J-P.; PRUDHOMME, M.; MORTIER-BARRIÈRE, I.; MARTIN, B. Adaptation to the environment: Streptococcus pneumoniae, a paradigm for recombination-mediated genetic plasticity?. Molecular Microbiology. Hoboken/Nova Jersey, v. 35, n.2, p. 251-259, 2000.
CLEWELL, D.B.; WEAVER, K.E. Sex. Pheromones and Plasmid Transfer in Enterococcus faecalis. Plasmid. Amsterdam, v.21, p. 175-184, 1990.
CLSI. CLINICAL AND LABORATORY STANDARDS INSTITUTE. Performance Standards for Antimicrobial Susceptibility Testing. Twenty-third
Informational Supplement: Supplement M100-S20, v. 33, n.1, 205 p., Wayne, PA, USA, 2013.
COBO, A.; ABRIOUEL, H.; OMAR, N.B.; LÓPEZ, R.L.; GALVEZ, A. Detection of ebp (endocarditis- and biofilm-associated pilus) genes in enterococcal isolates from clinical and non-clinical origin. International Journal of Food Microbiology. Amsterdam, v. 15, n.1-2, p. 123-126, 2008.
COCHETTI, I.; VECCHI, M. MINGOIA, M.; TILI, E.; CATANIA, M.R.; MANZIN, A.; VARALDO, P.E.; MONTANARI, M.P. Molecular characterization of
pneumococci with efflux-mediated erythromycin resistance and Identification of a novel mef gene subclass, mef(I). Antimicrobial Agents and Chemotherapy. Washington, v.49, n.12, p. 4999-5006, 2005.
COENYE, T.; GEVERS, D.; PEER, Y.V.; VANDAMME, P.; SWING, J. Towards a prokaryotic genomic taxonomy. FEMS Microbiology Reviews. Amsterdam, v. 29, p. 147-167, 2005.
COFFEY, T.J.; DANIELS, M.; McDOUGAL, L.K.; DOWSON, C.G.; TENOVER, F.C.; SPRATT, B.G. Genetic analysis of clinical isolates of Streptococcus pneumoniae with high-level resistance to expanded-spectrum cephalosporins. Antimicrobial Agents of Chemotherapy. Washington, v. 39, n.6, p. 1306– 1313, 1995.
COHAN, F.M.; KOEPPEL, A.F. The origins of ecological diversity in Prokaryotes. Current Biology. Amsterdam, v.18, p. 1024-1034, 2008. COLFORD, J.M.; SCHIFF, K.; GRIFFITH, J.F.;YAU, V. ARNOLD, B.F.;
WRIGHT, C.C.; GRUBER, J.S.; WADE, T.J.; BURNS, S.; HAYES, J.; McGEE, Ch.; GOLD, M.;CAO, Y.; NOBLE, R.T.;HAUGLAND, R.; WEISBERG, S. B. Using rapid indicators for Enterococcus to assess the risk of illness after exposure to urban runoff contaminated marine water. Water Research. Amsterdam, v. 46, p. 2176 - 2186, 2012.
COLLINS, M.D.; FARROW, J.A.; JONES, D. Enterococcus mundtii sp. nov. International Journal of Systematic Bacteriology Cambridge, v.36, n.1, p. 8- 12, 1986.
CONCEIÇÃO, N. Detecção da resistência às penicilinas por testes fenotípicos e à vancomicina por testes fenotípicos e genotípicos em amostras de Enterococcus. 90 f. Dissertação (Mestrado em Patologia Clínica), Universidade Federal do Triângulo Mineiro, Uberaba-Minas Gerais, 2008.
CONVERSE, R.R.; GRIFFITH, J.F.; NOBLE, R.T.; HAUGLAND, R.A.;
SCHIFFT, K.C.; WEISBERG, S.B. Correlation between Quantitative PCR and Culture-Based Methods for Measuring Enterococcus spp. over Various
Temporal Scales at Three California Marine Beaches. Applied and
Environmental Microbiology. Washington, v. 78, n. 4, p. 1237–1242, 2012. CORRÊA, A.B.; OLIVEIRA, I.C.; PINTO, T.C.A.; MATTOS, M.C.;
BENCHETRIT, L.C. Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 104, n.4, p. 599-603, 2009.
COSTA, P.M.; LOUREIRO, L.; MATOS, A.J.F. Transfer of Multidrug-Resistant Bacteria Between Intermingled Ecological Niches: The Interface Between Humans, Animals and the Environment. International Journal of
Environmental Research and Public Health. Washington, v. 10, p. 278-294, 2013.
COX, C.R.; GILMORE, M.S. Native Microbial Colonization of Drosophila melanogaster and Its Use as a Model of Enterococcus faecalis Pathogenesis. Infection and inmmunity. Washington, v.75, n.4, p. 1565–1576, 2007. CRAIG, W.A. Pharmacokinetic/pharmacodynamic parameters: rationale for antibacterial dosing of mice and men. Clinical Infectious Diseases.Oxford, v. 26, p.1–12, 1998.
CRETI, R.; IMPERI, M.; BERTUCCINI, L.; FABRETTI, F.; OREFICI, G.; Di ROSA, R.; BALDASSARRI, L. Survey for virulence determinants among
Enterococcus faecalis isolated from different sources. Journal of Medical
Microbiology. Amsterdam, v.53, p. 13–20, 2004.
D A EVED , P.A.; DIAS, C.A.G.; TEIXEIRA, L.M. Genetic dibversity and
antimicrobial of enterococcal isolates from southern region of Brazil. Revista do Instituto de Medicina Tropical. São Paulo, v. 48, n. 1, p. 11-16, 2006.
DADA, A.Ch.; AHMAD, A.; USUP, G.; HENG, L.Y. Antibiotic Resistance and Virulence Among Enterococci Isolated from Teluk Kemang Beach, Malaysia. Water Quality, Exposure and Health. Washington, v. 4, p. 113-122, 2012. DAVIES, C. M.; LONG, J.A.H.; DONALD, M.; ASHBOLT, N.J. Survival of Fecal Microorganisms in Marine and Freshwater Sediments. Applied and
Environmental Microbiology. Washington, v. 61, n.5, p. 1888-1896, 1995. DAVIES-COLLEY, R.J.; BELL, R.G.; DONNINSON, A.M. Sunlight inactivation of enterococci and fecal coliforms in sewage effluent diluted in seawater. Applied and Environmental Microbiology. Washington, v.60, n.6, p. 2049- 2058, 1994.
DAVIES, .A. SHANG, W. B SH, K. Activities of Ceftobiprole and ther β- Lactams against Streptococcus pneumoniae Clinical Isolates from the United States with Defined Substitutions in Penicillin-Binding Proteins PBP 1a, PBP 2b, and PBP 2x. Antimicrobial Agents of Chemotherapy. Washington, v. 50, n.7, p. 2530–2532, 2006.
De GRAEF, E.M.; DEVRIESE, L.A.; VANCNNEY, M.; M. BAELE.; COLLINS, M.D.; LeFEVRE, K.; SWINGS, J.; HAESEBROUK, F. Description of
Enterococcus canis sp. nov. From dogs and reclassification of Enterococcus porcinus Teixeira et al. 2001 as a junior synonym of Enterococcus villorum Vancanneyt et al. 2001. International Journal of Systematic and
Evolutionary Microbiology. Cambridge, v. 53, p. 1069–1074, 2003. DENAMIEL, G.; PUIGDEWALL, T.; TESTORELLI, F.; ALBARELLOS, G.; GENTILINI, E. Resistencia de Streptococcus bovis aislados de mastitis bovina frente a penicilina y macrólidos-lincosamidas. Investigación Veterinaria. Buenos Aires, v. 15, n.1-2, p. 137-141, 2013.
DESMARAIS, T.R.; SOLO-GABRIELE, H.M.; PALMER, C.J. 2002. Influence of soil on fecal indicator organisms in a tidally influenced subtropical environment. Applied and Environmental Microbiology. Washington, v. 68, p.1165–1172, 2002.
DHANARANI, T.S.; SHANKAR, C.; PARK, J.; DEXILIN, M.; KUMAR, R; THAMARAISELVI, K. Study on acquisition of bacterial antibiotic resistance determinants in poultry litter. Poultry Science. Oxford,v. 88, n.7, p. 1381-1387, 2009.
DHANDA, V.; VOHRA, H.; KUMAR, R. Group A Streptococcus virulence factors genes in north India & their association with emm type in pharyngitis. Indian Journal Medical Research. Nova Delhi, v.133, p. 110-115, 2011. DÍAZ, L.M.; SÁNCHEZ, M.J.T.; MARTÍN, J.A. Prevalence and mechanisms of erythromycin and clindamycin resistance in clinical isolates of b-haemolytic streptococci of Lancefield groups A, B, C and G in Seville, Spain. Clinical Microbiology and Infectious Diseases. Amsterdam, v.14, p. 85-87, 2008. DÍAZ, T.M; NIEVES, B.M. Comparación de medios de cultivo y procedimientos para detectar colonización por Streptococcus agalactiae en mujeres
embarazadas. Revista Chilena de Infectología. Santiago de Chile, v. 25, n. 2, p. 108-113, 2008.
DI LUCA, M.C.; D’ERC E, S. PE RE I, D. PRENNA, M. RIPA, S. VI A I, L.A. Lysogenic Transfer of mef(A) and tet(O) Genes Carried by Фm46.1 among Group A Streptococci. Antimicrobial Agents and Chemotherapy.
Washington, v. 54, n. 10, p. 4464-4466, 2010.
DIONISIO, L.P.; GARCIA-ROSADO, E.; LOPEZ-CORTES, L.; CASTRO, D.;BORREGO, J.J. Microbiological and sanitary quality of recreational
seawaters of southern Portugal. Water, Air and Soil Pollution. Washington, v. 138, p. 319-334, 2001.
DOERN, G.V.; FERRARO, M.J.; BRUEGERMANN, A.B.; RUOFF, K.L. Emergence of High Rates of Antimicrobial Resistance among
Viridans Group Streptococci in the United States. Antimicrobial Agents of Chemotherapy. Washington, v. 40, n.4, p. 891-894, 1996.
DORFMAN, M.; ROSSELOT, K.S. Testing the waters - A Guide to Water Quality at Vacation Beaches. Natural Resources Defense Council. Nineteenth Edition, 453p., 2009.
DORSEY, J.H.; CARTER, P.M.; BERGQUIST, S.; SAGARIN, R. Reduction of fecal indicator bacteria (FIB) in the Ballona Wetlands saltwater marsh (Los Angeles County, California, USA) with implications for restoration actions. Water Research. Amsterdam, v. 44, p. 4630-4642, 2010.
DSMZ- DEUTSCHE SAMMLUNG von MIKROORGANISMEN UND ZELLKULTUREN. Disponível em: http://www.dsmz.de/support/bacterial- nomenclature-up-to-date-downloads/readme.html. Acesso em: 18 FEV. 2015. DUFOUR, A.; WADE, T.J; KAY, D. Epidemiological studies on swimmer health effects associated with potential exposure to zoonotic pathogens in bathing beach water- a review. In: Animal Waste, Water Quality and Human Health – WHO- IWA publishing, London, UK, 2012.
DUNNE, E.M.; ONG, E.K.; MOSER, R.J.; SIBA, P.M.; PHUANUKOONNON, S.; GREENHILL, A.R.; ROBINS-BROWNE, R.M.; MULHOLLAND, E.K.; SATZKE, C. Multilocus sequence typing of Streptococcus pneumoniae by use of
mass spectrometry. Journal of Clinical Microbiology. Washginton, v.49, n.11, p. 3756–3760, 2011.
DUTRA, V.G.; ALVES, V.M.N.; OLENDZKI, A.N.; DIAS, C.A.G.; BASTOS, A.F.A.; SANTOS, G.O.; AMORIN, E.L.T.; SOUSA, M.Â.B.; SANTOS, R.; RIBEIRO, P.C.S.; CLEUBER, F.F.; ANDREY, M.; MAGALHÃES, K.; ARAUJO, A.A.; PAFFADORE, L.F.; MARCONI, C.; MURTA, E.F.C.; FERNANDES, P.C.; RADDI, M.S.G.; MARINHO, P.S.; BORNIA, R.B.G.; PALMEIRO, J.K.; DALLA- COSTA, L.M.; PINTO, T.C.A.; BOTELHO, A.C.N.; TEIXEIRA, L.M.;
FRACALANZZA, E.L. Streptococcus agalactiae in Brazil: serotype distribution, virulence determinants and antimicrobial susceptibility. BMC Infectious
Diseases. Washington, v. 14, 9p., 2014.
DWIGHT, R.H.; CAPLANS, J.S.; BRINKS, M.V.; CATLIN, S.N.; BUESCHER, G.; SEMENZA, J.C. Influence of variable precipitation on coastal water quality in southern California. Water Environment Research. Washington v. 83, n.12, p. 2121-2130, 2011.
EBRAHIMI, A.; NIKOOKHAH, F.;NIKPOUR, S.;MAJIIAN, F.; GHOLAMI, M. Isolation of Streptococci from milk samples of normal, acute, subclinical mastitis cows and determination of their antibiotic susceptibility patterns. Pakistan Journal of Biological Sciences. Camberra, v.11, n.1, p. 148-150, 2008. ELMIR, S.M; WRIGHT, M.E; ABDELZAHER, A; SOLO-GABRIELE, H.M; FLEMING, L.E; MILLER, G; RYBOLOWIKC, M; SHIHD, M-T. P; PILLAI, S.P; COOPER, J.A; QUAYED, E.A. Quantitative evaluation of bacteria released by bathers in a marine water. Water Research. Amsterdam, v. 41, n. 1, p. 3-10, 2007.
ENNS, A.A; VOGEL, l.j; ABDELZAHER, A.M; SOLO-GABRIELE, H.M;PLANO, L.R.W; GIDLEY, M.L; PHILLIPS, M.C; KLAUS, J.S;PIGGOT, A.M; FENG, Z; RENIERS, A.J.H; HAUS, B.K; ELMIR, S.M; ZHANG, Y; JIMENEZ, N.H;
ABDEL-MOTTALEB, N; SCHOOR, M.E; BROWN, A; KHAN, S.Q; DAMERON, A.S; SALAZAR, N.C; FLEMING, L.E. Spatial and temporal variation in indicator microbe sampling is influential in beach management decisions. Water
ERGIN, A.; ERCI, S.; HASÇELIK, G. Macrolide resistance mechanisms and in vitro susceptibility patterns of viridans group streptococci isolated from blood
cultures. Journal of Antimicrobial Chemotherapy. Oxford, v. 57, p.139–141,
2006.
FACKLAM, R. What Happened to the Streptococci: Overview of Taxonomic and Nomenclature Changes. Clinical Microbiology Reviews. Washington, v. 15, n.4, p. 613-630, 2002.
FACKLAM, R.R.; ELLIOT, J.A. Identification, Classification, and Clinical Relevance of Catalase-Negative, Gram-Positive Cocci, Excluding the
Streptococci and Enterococci. Clinical Microbiology Reviews. Washington, v.8, n. 4, p. 479–495, 1995.
FACKLAM, R.R.; COLLINS, M.D. Identification of Enterococcus Species Isolated from Human Infections by a Conventional Test Scheme. Journal of Clinical Microbiology. Washington, v.27, n.4, p. 731-734, 1989.
FACKLAM, R.R.; HOLLIS, D.; COLLINS, M.D. Identification of Gram-Positive Coccal and Coccobacillary Vancomycin-Resistant Bacteria. Journal of Clinical