CHAPTER III. Tubular fibrous scaffold functionalized with tropoelastin as a small-diameter vascular
3.6 Conclusions
We have successfully developed a biofunctional eTF scaffold for vascular applications with appropriate uniaxial tensile properties within the same range of those of native blood vessels. By tailoring the surface functionality, different interactions between the biofunctional substrate and endothelial cells can be achieved. Indeed, exposing tropoelastin -COOH groups to cells seemed to influence the endothelial cells behaviour towards enhancing its viability and activity. Otherwise, exposing its -NH2 groups stimulated endothelial cells to produce higher amounts of protein. After 7 days of culture, a confluent endothelial cell monolayer was present on the eTF scaffolds surface which promoted a rapid endothelialization. This study suggests that by combining the fibrous and porous structure, and the mechanical properties obtained from eTF scaffolds with biochemical cues provided by tropoelastin, it is possible to design a biofunctional and bioactive tubular scaffold as a valid alternative to functional engineered vascular grafts.
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