O presente estudo in vitro demonstrou que a adição de DHA e EPA ao diluente de criopreservação, nas concentrações testadas, não demonstrou efeitos deletérios ao sêmen de garanhões da raça Crioula.
Entretanto, os estudos publicados relatando os efeitos da adição destas substâncias na criopreservação de sêmen são escassos e controversos, especialmente na espécie equina. Investigações futuras são importantes e devem ser realizadas com intuito de demonstrar se a adição de PUFAs, em particular de DHA e EPA, é um método útil para melhorar a congelabilidade do sêmen equino ou não. Desta forma, avaliação de diferentes períodos e temperaturas de incubação do sêmen equino com lipídeos exógenos, além de diferentes concentrações e realização de quantificação dos lipídeos para constatar se os mesmos foram ou não incorporados à membrana plasmática do espermatozoide podem auxiliar em tal questão. Adicionalmnte, estudos in vivo podem ser importantes para avaliar a fertilidade do sêmen equino criopreservado, quando adicionado DHA e EPA ao diluente.
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