DISCUSSÃO GERAL

Nanopartículas de maguemita recobertas com citrato foram sintetizadas via co-precipitação aquosa – seguida pela oxidação e funcionalização com citrato. A camada de ouro foi depositada sobre o núcleo magnético pela redução dos íons Au3+ na presença de nanopartículas de γ-Fe2O3

durante dois ciclos de semeamento com boroidreto. Aumentando a concentração de Au3+ durante em cada semeamento, aumentando a razão Au/Fe2O3 (e logo a espessura da camada de ouro). O

recobrimento com ouro pode ser confirmado por vários ensaios. Particularmente, medidas ópticas mostraram um deslocamento para o azul dependendo da espessura da camada de ouro. Todas as amostras apresentaram uma longa estabilidade coloidal em pH fisiológico, confirmado por medidas de DSL, mesmo depois da funcionalização com ftalocianina de alumínio. Além disso, este estudo mostrou que a camada de ouro fornece mais biocompatibilidade para as nanopartículas de γ-Fe2O3

de acordo com a evolução dos testes de citotoxicidade in vitro. Diferentemente de outros trabalhos, em que a ftalocianina precisa de vários passos recobrimentos extras e/ou moléculas cruzadas para serem covalentemente ligadas as nanopartículas, aqui os resultados indicam um forte acoplamento entre PTC e a superfície da nanopartícula, por interações eletrostáticas, em um processo de um único passo. Adicionalmente as nanopartículas recobertas mostraram respostas como agentes de contraste para CT dependendo da razão Au/Fe2O3. Possíveis aplicações destas nanopartículas

núcleo-camada irão derivar da combinação das propriedades magnéticas do núcleo (maguemita) e das propriedades ópticas da camada (ouro). Do ponto de vista terapêutico, estas amostras podem oferecer uma plataforma para realizar hipertermia magnética (núcleo) combinada ou não com terapia fototérmica (camada) e/ou terapia fotodinâmica (+ftalocianina). Levando em consideração a abordagem para diagnóstico, os materiais aqui sintetizados podem ser utilizados concomitantemente como sondas e agentes de contraste em técnicas de imagens médicas in vivo.

Futuramente, algumas novas abordagens serão realizadas na tentativa de se aprimorar, testar e confirmar propriedades e aplicações de novos materiais e/ou materiais já produzidos. Neste contexto, pode-se abordar nanopartículas em formatos elipsoidais, testes de toxidade in vivo dos materiais adsorvidos com ftalocianinas em presença e ausência de luz, novas condições de síntese para controlar a aglomeração das moléculas de ftalocianinas adsorvidas e também testa-los em abordagens como micoCT, terapia fotodinâmica, fluorescência, magnetohipertermina, dentre outras mais.

ANEXO I

Anexo II

Anexo III

Comprovante de envio (submissão) do artigo para a revista Journal of Material Chemistry B.

Anexo IV

Artigo enviado no template da revista Journal of Material Chemistry B.

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