dRILL~ N~; BRONNESTAM~ R~ Immuno-elactrophoretic studv of

1960~

-:::AMPBELL, i'1~J~A~ ri light and ele-ctron m1crosc:ope studv of

blood vessels +rom the gengival tissues of non-diabetlc and diabetic patien~s~ ~ust .. der:r!;_d~, 16:235-239~ 1971.

:ARNEIRO,

^C:AVA DE MORAES. Radioautographic visualization of the c::ollagen metabolis the periodontal tis-sues of the mouse~

lQ_:833-48'1" 1965.

CHALLACOMBE~ S~ J.; RUSSEL~ MM W~; HAWI<ES~ .J. E.; BERGMEIER~

L.A.; LEHNER~ T. Passaqe of

immunoqlobulins

from plasma

to the oral cavitv in ^rhesus

Immunologv~35:923-31~ 1978~

-:HESLER, A~; TISLOWITZ, R~ Effect of allox:an diabetes on the qrowing rat~ Science,1Q1:468, 1945.

COHEN, D~W .. ; FRIEDMAN~ L~A~; SHAPIRO~ J.; KYLE, G.C.:

FRANKLIN~ S~ Oiabetes mellitus and periodontal disease:

two-vear longitudinal observations~ p:art

r.

J~_Periodont~ ~::!1.:709-12~ 1970~

COTRAN~ R~S.; SUTER~ E~R.; MAJNO, G~ The use of colloidal carbon -as: a tracer for 'lasc:ular injury~ A review~

Vascular Di seases, ::!-,! 107-27 ~ 1967.

COV!AN~ i=. G~ Aspectos fisiológicos de la diabetes

exper1mental qor aloxana~ 263-90~

1946.

CRESKOFF~ A. J. ; FITTZ-HUCH~,Jrw ~ FARRIS~ E.J.

;;ematologigy of the rat - Nethods and standards. In:

1\!ew York~ Mafner ?ublishtng Company~

1971. cap.14~ p~ 411-2.

OUFF, G.L.; STARR, H.

E;-:perimental :::tlloxan diabetes in

h.ooded

r-ats.

1944.

DULIN. W.E~; SORET~ i"LG~ Sheancally and hormonally induced diabetes. In: The diabetic a~~· Eds. 8.W.Volk~

i<.F.Wellmann. New York eand London. Plenum Press~ 1977.

-o~ 425-65.

Necrosis of the islets of Lanqerhans oroduced exper1mentally. J. Path~Bact .. ""55!245-57~ 1943.

EGELBERG • .J. The blood vessels o+ the dento-qlngtval ,tuncti on~ ~rtodont. Res~ ~ _!_: 163-74, 1966a~

EGELBERB. J. Permeabilitv of the dento-gingíval blood vessels-., I~ Appic:ation o+ the vascular labelling method and g1nqival fluid measurements4

1:180-91~ 196ób.

,J ~ periodont ~ Res.

•

79

EGELBERG. J.

Permeabilitv o+ the dento-qingival blood

vesse!s-~ I I. Clinicallv health ginq1vae.

EGELBERG~ J. Permeabilltv of the dento-gingival blood

vessel~h .ti I. ChronicallY inflamed ging1vae~

~GELBERG, ,l. ?ermeabi li ty of the dento-gi nqi val bl oad vessels. IV. Effect of histamine on vessels i.n c.l1n1cally healthy and chronically inflamed g1nqivae.

J.geriodonj:.Res$ ~_t:297-302~ 1966e.

EGELBERG,

J.;

ATTSTROM, R.

Comparison between orifíce and intracrevic:ular methods of samoling gingival

,J~ eeriodont~ Res~ ~

ª:

^{384-8, 1973~}

flui d.

E:LGENEIDY~ A-~K.; STALLARD. R~E.; FILLIOS~ L.C~; GOLDMAN, H.M. Per1odontal anti vascular alteratíons: their relationship to the changes in ·tissue- glucose and ']lycogen in di.abetic m1ce ..

.1974~

,J. Per i odont. ~ 45! 394-401 .

ERVASTif

T.~ KNUUTTILA~

M.;

POHJAMG~

L.; HAUKIPURO,

K~

Relation between control of diabetes dnd " gingival bleeding. J.Periodont~,~6:154-57~ 1985.

FRANTZIS, LG.; REEVE. G.M.:; BROWN~Jr •• A.L~

~:1 trastructure of c ao i l l ary basement membranes tn +:.h e

attached gi ngi v a o-i di abeti c ;an nondi abetic pat lents Wl th periodontal disease~ J~~rtoqont~~42:406-11, 1971.

3ANDA. O. P; ROSSINI ~ 3tudies on

strentozotocin diabetes* Diabet~?z~25_:595-603~ ~976~

3ANDA. CI.P. Pathoqenesis of macrovasc:ular disease 1n the :,uman diabetíc. A review. Diabetes,29:931-42~ 1980.

GARCIA-LEME, J.; HAMAMURA, L.; t1IGL!OR!Nl, R. H.; LEITE, !1. P.

Influenc:e of diabetes upon the inflammatorv response o-f the rat. A pharmacoloqical analysís. Eur. J. Phar-m., 23:74-81' 1973.

GARCIA-LEME~ J ~; BÜHN~ G~ M~; MIGLIORINI ~ R. H.~ DE SOUZA.

-,'1~LA. PossJ.ble- participation af l.nsulin in the contrai ,::Jf '-.tasct.Jlar permeabi 1 i ty.

1974.

Sur. J. Pharm., 29:298-306~

GLICKMAN~ L The periodontal st·ructures in J?}4PEr1mental diabetes. N. Y .. ^{J~dent~ ~} 16:226-51, 1946.

GL!CKMAN~ !.: SMULOW, J.B.; MOREAU. J.

periodontal healing in alloxan diabetes.

1967.

Postsurgical J ^~ Per1 odont ^{~ ~}

ill

SdEB~ M.F. Q_Q,g_~olvimento do Qroc:esso inflamatório ....!.lQ.

tec:tdo__g_englval do rato. Pi.racícaba-SP~ 1981 .. 67p~ <.Tese de mestrado Faculdade de Odontologia de Piracicaba UN!CAMP>.

-JOES~ M.F.; ALMEIDA~ O~P.; BOZZO, L.; V!ZlOL!, r1. R~

Iascular cilanqes durinq the- •jeveiopment o-f the rat c:;pmp v a. ^,j. per1odon1:. Re-s~ ~ 18:402-11, 1983.

i·Hlox:an diabetes in the doq.

1943.

GOLUB~ L.M.~ GARANT~ P.R~; RAMAMURTHY~

N.S. In-tlammatorv

<:hanges in qing1val collagen 1n the- .allm:an-diabetic rat~

J.gerlodont.~Res.,12:402-18. 1977.

GOMORI~ G~; GOLDNER~ M.G. Production o+ diabetes mellitus :n rats 41th ""llaxan~ Proc. Soe. exp ~ Bi ol. ~54: 287-,::m,

80TH~ A.~ NASH~ W~ L.; NAGLER, M .. ; HOLMAN, J., lnhibition o.f h i staon ne rele-ase e>~perl mental

Am~ ,J ^» Physi ol ~ ~ 191: 25-8~ 1957.

GRANT~ R~Tv Direct observations of ske1etal muscle bload

---te-ssels (rat cremaster). ^123-37.

t964~

The effec:t of

topical insulin on infected cutaneous ulcerations tn d:iabetic and nondiabetic míc:e~ J~of

Foot

Surg~,22:

298-301' 1983 ..

~OLMBERG,

K.; KILLANDER, J.

Quantitative determination of

tmunoglobuline \IgG. IgA and IgMl and

identification

of

IgA type in the ging1val fluid.

t 971.

HOUSE. ^{E.L~ ~} histolog1cal study of the ^pancreas~ liver and i< i dne-v both ,juri ng and a f ter recovery from all oxan diabetes. ~ndoc:rinalogy,62:189-2úO. 1958 ..

HOVE~ JC A~; STALLARD, R~ E.

Diabetes and the periodontal

1970.

JACOBS. Hypogl i cemH.: o1ction o f ""lloxan~

f'roc~Soc.exp.Biol.Med«~37:407-9, 1937.

JOHNSON~ R~B. Effects of exper1mental diabetes mellitus on alveolar bone loss in periodontal disease-susceotlble mice. J~geriodont~Res~,20:307-16,. 1985~

<ASS. E.H.;

WA!SBREN.

B.A. A method for c:onsi stent :nduction of chr-onu: hyperglicemia with' alloxan.

F'rnc~Soc~exp .. Biol ~Med~ ,~:303-6, 1945~

83

KEENE, Jr~ ~ J.J ...

Observations of small blood vessels

1n

;-tuman nondiabetic -and diabetic gingiva~

1969.

L~;

VICTOR..-

J ~ Alloxan diabetes ln rabbits~ Product1on of hypercholesterolemia~

hvperlipemla 2.nd ~drenai c::ortical 1 esi ons~

:::•roc. ^Soe~ exg. Bi ol. Med. ^~ 60: ^190-5~ 1945~

GILL~ A .. J.; HARRIS~

L. C.

Gl ycogen ::.n alloxan-treated rats~ ?roc. Soe. exP-.•

1944~

The ;:woduction and course of allo,tan diabetes in the rat~

1004-15~ 1946~

LAZAROW. A~ Spontaneous recovery from alloxan diabetes 1n

the- rat. Diabetes, .V 363-72. ^1952~

LEWIS, L.A.l MOSES, .J.; SCHNEIDER, R.W. Plasma proteins~

ti~Alteration in alloxan diabetic rabbitts especially in relation to ocular damage~

!947.

LISTGARTEN~

M.A; RICKER,

Jr~~ F.H~;

LASTER, L.;

SHAPIRO~ J~;

COHEN~

D.W.

Vascular basement lamina thickness in the normal and in-flamed gingi v a o f diabetics and

non-oiabetics. J~eriodont~~45:676-84, 1974.

~Ul<ENS. ~'.D.W. ~lloxan diabetes. _304-30~

1948.

MAJNO, G~; PALADE~ Studies wn in-flamation. I. The -effect

ot

h i stam1 ne and serotonin on ·.rasc:ul ar oermeabi 1 i tv: ~lectron :n1 croscop1 c study.

MAJNO.

_-::l ^~ _{* •}

.

_{G. E~ ; SCHOEFL, Studies C)(l.}

inflammat1on~ IIQThe site of action of histamine and

.:erotonín along the vascular

tree: a topographrc study.

J ~ Bi oohys. Bi ochen:t~ Cvtol_. ~LV 607-·26~ 1961.

MAJNO, G.: GILMORE, V.; LEVENTHAL,

A technique for the

·nicroscopic study of blC<od vessels 1n liv1.ng ;;;triated

:nuscle (cremasterJ. 1967-a.

~AJNO. G~~ G!LMORE~

V.{

LEVENTHAL~ On the mechant sm of vascular l eakaqe caused by h i stamine-type medi dtors~ ""~

mi croscop1 c studv ''in vivo''. Circulation Re~ .• 21:

933-47. 1967b~

MANSFORD. ^{OPIE. L.}

Compari son of metabolic

-~bnormal i t i es in d1abets mellitus induced streptozotoc2n or by alloxan~ Lancet, 1.:670-1 ~ 1968.

MC DOUGALL1 Penetration pathways of :a tooicallv

"'pplied foretqn prote:tn Lnto q1nqiva.

1971 ~

85

1C MULLEN, ,J.;<.; LEGG, M.; GOTTSEGEN, R.; CAMER!NI-OAVALDS,

.R~ Microanqiooathv within the cpnqival tissue-s of diabetic -subjec:ts Wlth spec1al reference to the

orediabetic

state~ Periodontics~~:61-9~ 1967.

''4C NAMARA. T. F.; RAMAMURTHY,. N. S .. ; MULVIHILL~ J ~E~; GOLUB, The ,:fevel opmem: :Jf an al tered gi ngi val c:revicul ar -1ncroflora 1n the alloxan-diabetic rat. Hrchs. oral

1982.

1ER!NG e MlNr:OWSK! i 1889) apud F.G. COVIAN 1n "Aspectos .;:lsioiàgicos de la diabetes experimental por la aloxana''.

o. 263. Cadern-~ C i ent. ~

1:

263-90~ 1946~

''iOWAT ~ A.G.; BAUM~ J ~ ⁰ olymorohonuclear leucocvtes from pa:tients with diabetes mellitusw

b21-?~ 1971.

:_esties oeriodontals em ratos diabéticos

Rev.Paul.Odontol~,ª:S-18~ 1985.

NICOLAU" J.;

^ROSA~

R ... ; FAVA-DE-MORAES,.

^F~

aloxàn1cos~

The effect of

::..lloxan

diabetes

^~~1pon n-acetylneuraminic acid Acta -::oncentration tn the submaxillarv glands of ra'ts~

1969.

the teeth and other tissues following alloxa.n tnjection~

1948.

PETTIGREW, Jr ~ ~

SNYDER. M.B.

RABINOWITZ.

J~L-~ 4 bitewing radiographic studv o+

oopulatíon of aiabetic and nondiabetic rnesus monkeys.

J.P~_odontol~ .51;693-9~ 1980~

c;;AMAMURTHY~

N.S.; ZEBROWSI<V, E.J.; GOLUB, L.M. _{The effect}

of allo~an diabetes on g1ng1val collagen metabolism 1n rats. -4rrhs.oral Biol~.t7:1551-60, 1972.

RAMAMURTHY. ^N~S~; lEBROWSKI, E.J.; BAI<ER.

Hll m: an ,ji abetes and reduced

bane density in rat

1973.

RAMAMURTHY~ N.S.~ Insulin

re-versal of alloxan-diabe-tes induced changes in gingival collagen metabo1ism of the rat~ ,] ~per 1. odont. Res~ ,

2.!

199-206, 1974.

RAMAMURTHY~ N.S~; GOLUB. L.M~ Collaqen bicsynthetic enzime i!.ctivitv 1n diabetic rat ginqiva. J.dent.Res.~ s-oec:ial

lSSUE' A~ 311. 1978.

87

RAMAMURTHY, N.S.; SIEGEL, M.; !ACDNO, Y.J.; GOLUB, L.M.

Leuc:ocvte response 1n the gimp val crevice- o+ the

1979~

RAMAMURTHY, N.S.;

GOLUB, L.M. Diabetes 1nc:reases ,:ollaqenase activity 1n e-xtracts of rat ging1va and skin,.

hp&rlodont~ Res.~, 18: 2::5-30~ 1983v

:::AMAMURTHY~ N.S.~ GREENWALD~ R~A.; SCHNEIR~ M.; GOLUB, L.M.

The e-ffect of alloxan diabetes on prolyl and lvsil hvdrox:ylase activity in uninflamed and inflamed rat 91ng1va. Archs~oral Biol~~·30:679-83~ 1985~

RENOLD, A~E.; BURR~ I.M.; STAUFFACHER. W,. Experimental and spontaneus diabetes in animals: what is their relevance to human diabetes mellitus? ?roc~~ov.Soc.Med.,64:

613-17~ 1971.

qERUP.

c.;

^{TARDING~ F.} Streptozotocin and alloxan-diabetes

1969~

REUTERVING~ C.O~; HAGG~ E~: GUSTAFSON~ G~T~ Root surface caries and periodontal disease 1n long-term alloxan diabetic rats. J.dent~Res~~65:689-94, 1986.

ROBBINS, S~L- Patologia estrutural e funcionai~ Rio de

.Janeiro~ Interameric:ana~ 1974" 1422p ..

ROSE~ H. The relationship of hyperglycemia to periodontal di se a se. .J * Per1.odontol. , 44: 303-8~ 1973~

RUANGSlRl, C. Changes in i slets of Langerhans in li ^Vl nq mice after alloxan admini stration ... Anat.Rec.~!_05:

399-427, 1949.

RUBEN~ J. ^A.~ YARDUMIAN~ l<~ 'Jiabetes produced by +eeding

~lloxan to cats. 3cience~103:220-1~ "!.946.

RUSSEL. B. G. Gingival changes in diabetes mellitus~

1 *Vascular changes~ Acta ó8: 161-8. 1966~

3CHER, S.M.; LAWRENCE. A.M.

alloxan diabetic ^rats~

Functional micro.angiopathy :n Meta

1971.

Extensive degradation of recently '5ynthetized collagen in gingiv.a of normal and streptozotocin-induced diabetic ^rats,.

J~dent.Res. f63!23-7~ 1984.

SCHNEIR, M.L.; IMBERMAN, M.; RAMAMURTHY, N.S.< GOLUB, L.M.

The "in vivo" fracti onal r ate o f gi ngl vai coll agen production non-diabetic and di abectj_c rats~

1986.

SCHOEFL,. Studies

on

lnflammation~ ! 1! . Grovn ng caotllarles: theH" -structure a.nd permeat:n 11 t.v~

1962.

3CHWARTZ, J.; ST!NSON, F.L.; PARKER, R~B. The passaqe of tr1tiated bactertal endotox in acr-Clss 1 ntact ^~1 ng:t val

crev1cular eoithel1um~ t972.

3EIFERT, DIXIT~

m1gration inhibition 1n _diabetes~

3HAFER. iJJ.G. ~ HlNE, t1.K.; LEVY.

bucal. 4.etL Rio de Janeiro, Interameri.c:ana, 1985. ^-837o-~

:3HEPPARD, I. M. Oral marnfestation-s of diabetes mellitus:

study of one hundred cases.

~ 188-92~ 1942~

:3TAHL~ S~S. The periodontal struc:tures ln diabetic ,ntce~

1968~

3:ZNA-JDER ~ N. ; CARRARO, RUGNA. SEREDAY, ^M,

Per i odon tal ^findings in diabetic ^and nonoiabetic patients.

7HEILADE~ EGELBERG~ ATTSTRÔM, iasc:ul ar

oerme-aoility to collOldal r::arbon 1.n chron1cally inflamed 1971,

g:tngival flui d and olasma ^by crosserl

1mmunoelectrophoresis~

t980.

"OLO, K.J. ~ study e~f permeabi li ty oi gengiva! pocket ::?DlthelltJm to album1n tn '~Lunsa p1qs i<nd Norweqtan 01qs.

WATANABE~

G.; INGALLS,

T~H.

Congenital malformations in the

0ffsor1ng o~ ~lloxan-oiabetic m1ce.

t963~

WED~lORE.

c. v.

^~ >HLLIAMS~ T. J. Control o f vascul.a.r qermeability

o v

oolymorphonuclear ieukocvtes in

1r1flammation~ Nature,;289: 646-50~ 1981~

:MEEl<S. W.T~; '31MS~ M~R. 'he vasc:ulature of the rat molar g1nqival crevice. ,;I. per1. odont. Re!§.~ ~ 21! 177-85~

The vasct.üature of the r.at 'flol ar oer1odontal ^{ligament~ J ..} perx odont. Res. ~ 21: 186-94~

\986b.

i,.JQEHLER~ F~ (1838} apud F.D. LUkENS in "Alloxan diabetes~"

f'hVSlOl~Rev~!28!304-30. 1948.

'.J1EIRA~ S .. Rio de

---.iane1ro~ Ed.Camous Ltda~. 1983. 294p.

Z1MMERMAN,. A. L~; 3ABLAY~

L.B .. ; riYNEDJIAN, H.S.;

BANK~

oer:t tom;;-al oermeao:tli.ty Wlth

T?O-G, :.984.

!O. AP,ND!CE.

Determinaç~o

da Glicemia.

--'1 glicem1a. foi medida oor dois métodos; com a utilizaió:ào Glic:o-Teste e através método da Jrto-Toluídina. Ta1s métodos e resultados

do

silo descrttos

•este -?.nexo.

:::!é todo ....:.';1..9,_ Aparei h o Gl i r o- Teste4

quant.i-ficaç~o da glicose ~:em o aparelho Glico-Teste

·ExataJ. se bese1a na ~ei-tura esoectrofotométr1ca de

LaboratOrios Miles) para 2nz1ma -glicose-O}{Ídase~ especifica --:::;li cose.

-:::at:al i s-a a glicose no sangue oelo

,;,;c:mosfértco, oroduzindo-se àc2do qlict'ln2t:0 e oeróxido

:"ll droq!õl:ni o~ A oeroxtdase catalisa a rea~~o do peróxido com

.:m s1stema cromoq~nio~ produzindo cromoqênios oxidados que

~oresentam cores características~ e que de acordo com qlicose oroduzem coloraç;:Cl'es ma1s tntensas.

estas de 1 idas pelo

?'Soectrofotometro do aparelho~ com a leitura dada em mg'l.~

:.'btidos oelo método do aoaretho Gl i co-Teste e o método ,ja ')r to-T ol ui di na~ em 10 r atos normais e ₁₀ diabéticos.

;;;:.ode-se observar nesta tabela~ que .aoesar dos resultados

t::.a1s dtferenças nào apr·esentar-:3-m-se sign1ficat1vas~

TABELA 1 i'1édi-as e Desvio-Padrào .:;ias concen-traçdes de qlicos.e no sangue de t-atos normais e diabéticos obt1dos pelo .nêtodo da Orto-TolLtidina e pelo aparelho Gllco-Teste~

"---·---,':\ATOS :JRTO- TOLUIDINA GLICO-TESTE

i soro} i sangue total)

\IOR!"1AIS ín=10) 119,15±14.36 108,30± 6,25 DIABéTICOS \n=10l 367.29'!"42.82 .350. 60:t20, 67

A tabela 2 apresenta a glicemia de õ r~tos normais e 6 .jlabêticos medidas oelo aparelho Glico-Teste:~ com difere-ntes períodos de jejum dos animais~ Nesta tabela~ para ~ medida :j-a gl.icosória, utilizou-se Glicc-Fita ~Llll,;l.

NORMAIS (n""6) DIA lCOS ( n""6

dlab~tlcus,

ent

dlfer&nLes

p=rloJos

de horas apÓs

a'lilllentação.

'0

'-"

::. !'jêtpdo da Orto-ToluidinQ.

Foi utilizado o conjt.mto de reagentes "bi o8RAS" no qual o fundamento do método se baseia em que a glicose em '1'\eno àcido e sob aquecimento" através de seu grupamento al dei di co~ condensa-se com o grupamento amina da orto-toluidina. levando á formac.êro da mistura em equilíbrio,

-::orr<?spondente base

qlicosll-orto-to.luidina. Esta apresenta color.a;:ào

azul-esverdeada~ cuja intensidade é diretamente proporcional á concentr?t:ii:o de qlicose presente na amostra e possu1.

de -3.bsor~~o mà}Offia em 630 nm~

pico

Utilizou-se para obtenç2fo das medidas glicêmic:as Q

plasma \fluon.'!'-to de sódio como anticoagulante) e o soro de

---minutos~

e

^as

No documento PERMEABILIDADE DOS VASOS GENGIVAIS EM DIABÉTICOS ALOXÂNICOS (páginas 86-106)