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Supplementary data
Table S1. Distribuition and frequencies of the haplotypes (cpDNA) in all Dyckia populations sampled Haplotype Code Haplotype composition* Population Frequencies
Haplo-1 97 92 76 85 78 66 79 98 Brejo 0.70 Haplo-2 97 92 76 86 78 66 79 98 Brejo 0.30 Haplo-3 98 91 75 86 79 68 79 99 Mr 0.11 Haplo-4 98 91 75 86 79 68 81 99 Ca/Mr 0.74 / 0.44 Haplo-5 98 91 75 86 79 72 80 99 Lajes 0.11 Haplo-6 98 91 75 86 79 73 79 96 Lajes 0.22 Haplo-7 98 91 75 86 80 72 79 100 Ca/Lajes 0.21 / 0.22 Haplo-8 98 91 75 86 80 72 81 100 Lajes 0.05 Haplo-9 98 91 75 86 81 73 79 99 Lajes 0.11 Haplo-10 98 91 76 85 78 67 79 98 Pico 0.10 Haplo-11 98 91 76 86 79 72 79 99 Mr 0.11 Haplo-12 98 91 76 86 79 72 81 99 Mr 0.11 Haplo-13 98 91 76 86 79 73 79 96 Lajes 0.22 Haplo-14 98 91 76 86 79 73 79 99 Lajes 0.11 Haplo-15 98 91 76 86 80 72 79 99 Mr 0.11 Haplo-16 98 91 76 86 80 72 81 99 Mr 0.11 Haplo-17 98 91 77 85 78 67 79 98 Pico 0.40 Haplo-18 98 91 77 86 78 67 79 98 Pico 0.40 Haplo-19 98 92 77 85 78 67 79 98 Pico 0.10 Haplo-20 99 91 76 85 78 62 79 98 Cat 0.20 Haplo-21 99 91 76 85 78 62 80 98 Cat 0.10 Haplo-22 99 91 76 85 78 66 79 98 Cat 0.70 Haplo-23 99 91 76 85 78 67 79 98 Srita 1.00 Haplo-24 99 91 77 85 78 66 79 97 Pesq 0.70 Haplo-25 99 91 77 86 78 66 79 97 Pesq 0.30
* The haplotypes are characterized by their fragments sizes, in base pairs, across the eight plastid loci Ca, “Cachoeira” population; Mr, “Morrão” population; Cat, “Catimbau” population
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Figure S1. Weighted Neighbour joining dendrogram based on genetic distance of 15 nuclear SSR loci in populations of the three Dyckia species. Numbers are bootstrap support values.
Figure S2. Indication of the most likely number of clusters after Evanno et al. (2005) in the STRUCTURE analisys. Results from 10 replicates for each 1≤ ∆K ≤10 values with both AFLP (A) and nSSRs (B). ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! !
Conclusões Gerais
• Os resultados, quanto à reconstrução filogenética aqui apresentados, forneceram os
primeiros insights em nível infra-genérico no gênero Dyckia, reconhecido pela sua extrema diversidade morfológica e diversificação recente. Além disso, mesmo apresentando-se como um grupo claramente monofilético, o baixo suporte nos clados basais indica que, possivelmente, as barreiras reprodutivas inter-específicas ainda não estão inteiramente definidas. Desta forma, eventos de hibridizações e introgressões parecem ser comum em Dyckia, principal causa, portanto, do baixo sinal filogenético encontrado nas reconstruções realizadas. !
• O conjunto de primers desenvolvidos (nucleares e plastidiais) mostraram-se promissores candidatos nas análises populacionais tanto dentro do gênero Dyckia como em outros grupos dentro da família Bromeliaceae, tendo em vista os elevados indices de amplificação heterólogas observados.
• Considerando os resultados populacionais apresentados, mediante a análise agregada de marcadores dominantes (AFLP) e co-dominantes (microssatélites), com particular histórias evolutivas dentro do genoma, notificou-se a significativa eficácia de tais marcadores em revelar padrões de conectividade e diversidade em populações de
Dyckia !
• Os dados moleculares em conjunto com as características morfológicas apresentadas pelas populações de Dyckia, ocorrentes nos inselbergs de Pernambuco, não puderam confirmar D. limae como uma unidade taxonômica definida, em relação as demais populações de D. pernambucana. Por ser micro-endêmica do PARNA do Catimbau, localizado na cidade de Buíque, e dada a natureza intrínseca do ambiente (única formação sedimentar do Planalto da Borborema), as distinções morfológicas encontradas possivelmente podem ser explicadas pelas particularidades adaptativas a tal formação rochosa elevados indices de diferenciação genetica.!
! ! !
Anexos