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Anexo 1: Normas da revista PLoS ONE, a qual o artigo será encaminhado para publicação.
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Related information for authors
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- Submission system
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- Journal scope and publication criteria
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- Getting started guide
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- Guidelines for revisions
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- Publication fees
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- Chinese translation of PLOS policies: PLOS编辑与出版规定
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Style and Format
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File format
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Manuscript files can be in the following formats: DOC, DOCX, or RTF. Microsoft Word
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documents should not be locked or protected.
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LaTeX manuscripts must be submitted as PDFs. Read the LaTeX guidelines.
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Length
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Manuscripts can be any length. There are no restrictions on word count, number of figures, or
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amount of supporting information.
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We encourage you to present and discuss your findings concisely.
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Font
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Use a standard font size and any standard font, except for the font named “Symbol”. To add
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symbols to the manuscript, use the Insert → Symbol function in your word processor or paste in the
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appropriate Unicode character.
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Headings
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Limit manuscript sections and sub-sections to 3 heading levels. Make sure heading levels are
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clearly indicated in the manuscript text.
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Layout and spacing
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Manuscript text should be double-spaced. Do not format text in multiple columns.
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Page and line numbers
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Include page numbers and line numbers in the manuscript file. Use continuous line numbers (do
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not restart the numbering on each page).
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Footnotes
Footnotes are not permitted. If your manuscript contains footnotes, move the information into the
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main text or the reference list, depending on the content.
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Language
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Manuscripts must be submitted in English.
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You may submit translations of the manuscript or abstract as supporting information. Read the
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supporting information guidelines.
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Abbreviations
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Define abbreviations upon first appearance in the text.
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Do not use non-standard abbreviations unless they appear at least three times in the text. Keep
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abbreviations to a minimum.
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Reference style
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PLOS uses “Vancouver” style, as outlined in the ICMJE sample references. See reference
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formatting examples and additional instructions below.
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Equations
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We recommend using MathType for display and inline equations, as it will provide the most
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reliable outcome. If this is not possible, Equation Editor or Microsoft's Insert→Equation function is
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acceptable.
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Avoid using MathType, Equation Editor, or the Insert→Equation function to insert single variables
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(e.g., “a² + b²= c²”), Greek or other symbols (e.g., β, Δ, or ′ [prime]), or mathematical operators
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(e.g., x, ≥, or ±) in running text. Wherever possible, insert single symbols as normal text with the
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correct Unicode (hex) values.
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Do not use MathType, Equation Editor, or the Insert→Equation function for only a portion of an
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equation. Rather, ensure that the entire equation is included. Equations should not contain a mix of
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different equation tools. Avoid “hybrid” inline or display equations, in which part is text and part is
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MathType, or part is MathType and part is Equation Editor.
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Nomenclature
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Use correct and established nomenclature wherever possible.
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Units of measurement: Use SI units. If you do not use these exclusively, provide the SI value in
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parentheses after each value. Read more about SI units.
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Drugs: Provide the Recommended International Non-Proprietary Name (rINN).
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Species names: Write in italics (e.g., Homo sapiens). Write out in full the genus and species, both in
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the title of the manuscript and at the first mention of an organism in a paper. After first mention, the
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first letter of the genus name followed by the full species name may be used (e.g., H. sapiens).
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Genes, mutations, genotypes, and alleles: Write in italics. Use the recommended name by
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consulting the appropriate genetic nomenclature database (e.g., HUGO for human genes). It is
sometimes advisable to indicate the synonyms for the gene the first time it appears in the text. Gene
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prefixes such as those used for oncogenes or cellular localization should be shown in roman
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typeface (e.g., v-fes, c-MYC).
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Allergens: The systematic allergen nomenclature of the World Health Organization/International
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Union of Immunological Societies (WHO/IUIS) Allergen Nomenclature Sub-committee should be
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used for manuscripts that include the description or use of allergenic proteins. For manuscripts
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describing new allergens, the systematic name of the allergen should be approved by the
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WHO/IUIS Allergen Nomenclature Sub-Committee prior to manuscript publication. Examples of
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the systematic allergen nomenclature can be found at the WHO/IUIS Allergen Nomenclature site.
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Copyediting manuscripts
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Prior to submission, authors who believe their manuscripts would benefit from professional editing
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are encouraged to use language-editing and copyediting services. Obtaining this service is the
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responsibility of the author, and should be done before initial submission. These services can be
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found on the web using search terms like “scientific editing service” or “manuscript editing
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service.”
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Submissions are not copyedited before publication.
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Submissions that do not meet the PLOS ONE publication criterion for language standards may be
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rejected.
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Manuscript Organization
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Manuscripts should be organized as follows. Instructions for each element appear below the list.
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Beginning section
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The following elements are required, in order:
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- Title page: List title, authors, and affiliations as first page of manuscript
668 - Abstract 669 - Introduction 670 Middle section 671
The following elements can be renamed as needed and presented in any order:
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- Materials and Methods
673 - Results 674 - Discussion 675 - Conclusions (optional) 676 Ending section 677
The following elements are required, in order:
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- Acknowledgments
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- References
- Supporting information captions (if applicable)
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Other elements
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- Figure captions are inserted immediately after the first paragraph in which the figure is cited.
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Figure files are uploaded separately.
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- Tables are inserted immediately after the first paragraph in which they are cited.
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- Supporting information files are uploaded separately.
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Please refer to our downloadable sample files to ensure that your submission meets our formatting
687
requirements:
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- Download sample title, author list, and affiliations page (PDF)
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- Download sample manuscript body (PDF)
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- Viewing Figures and Supporting Information in the compiled submission PDF
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The compiled submission PDF includes low-resolution preview images of the figures after the
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reference list. The function of these previews is to allow you to download the entire submission as
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quickly as possible. Click the link at the top of each preview page to download a high-resolution
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version of each figure. Links to download Supporting Information files are also available after the
695 reference list. 696 Parts of a Submission 697 Title 698
Include a full title and a short title for the manuscript.
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Title Lenght Guidelines Examples Full title 250
characters
Specific, descriptive, concise, and
comprehensible to readers outside the field
Impact of cigarette smoke exposure on innate immunity:
A Caenorhabditis elegans model Solar drinking water disinfection (SODIS) to reduce childhood diarrhoea in rural Bolivia: A cluster- randomized, controlled trial
Short title 100 characters
State the topic of the study
Cigarette smoke exposure and innate immunity
SODIS and childhood diarrhoea
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Titles should be written in sentence case (only the first word of the text, proper nouns, and genus
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names are capitalized). Avoid specialist abbreviations if possible. For clinical trials, systematic
702
reviews, or meta- analyses, the subtitle should include the study design.
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Author list
- Authorship requirements
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All authors must meet the criteria for authorship as outlined in the authorship policy. Those who
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contributed to the work but do not meet the criteria for authorship can be mentioned in the
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Acknowledgments. Read more about Acknowledgments.
708
The corresponding author must provide an ORCID iD at the time of submission by entering it in the
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user profile in the submission system. Read more about ORCID.
710
Author names and affiliations
711
Enter author names on the title page of the manuscript and in the online submission system. On the
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title page, write author names in the following order:
713
- First name (or initials, if used)
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- Middle name (or initials, if used)
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- Last name (surname, family name)
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Each author on the list must have an affiliation. The affiliation includes department, university, or
717
organizational affiliation and its location, including city, state/province (if applicable), and country.
718
Authors have the option to include a current address in addition to the address of their affiliation at
719
the time of the study. The current address should be listed in the byline and clearly labeled “current
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address.” At a minimum, the address must include the author’s current institution, city, and country.
721
If an author has multiple affiliations, enter all affiliations on the title page only. In the submission
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system, enter only the preferred or primary affiliation. Author affiliations will be listed in the
723
typeset PDF article in th same order that authors are listed in the submission.
724 725 726
- Author names will be published exactly as they appear in the manuscript file. Please double-
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check the information carefully to make sure it is correct.
728
Corresponding author
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The submitting author is automatically designated as the corresponding author in the submission
730
system. The corresponding author is the primary contact for the journal office and the only author
731
able to view or change the manuscript while it is under editorial consideration.
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The corresponding author role may be transferred to another coauthor. However, note that
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transferring the corresponding author role also transfers access to the manuscript. (To designate a
734
new corresponding author while the manuscript is still under consideration, watch the video tutorial
735
below.)
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Only one corresponding author can be designated in the submission system, but this does not
737
restrict the number of corresponding authors that may be listed on the article in the event of
738
publication. Whoever is designated as a corresponding author on the title page of the manuscript file
will be listed as such upon publication. Include an email address for each corresponding author
740
listed on the title page of the manuscript.
741
Consortia and group authorship
742
If a manuscript is submitted on behalf of a consortium or group, include the consortium or group
743
name in the author list, and provide the full list of consortium or group members in the
744
Acknowledgments section. The consortium or group name should be listed in the manuscript file
745
only, and not included in the online submission form. Please be aware that as of October 2016, the
746
National Library of Medicine’s (NLM) policy has changed and PubMed will only index individuals
747
and the names of consortia or group authors listed in the author byline itself. Individual consortium
748
or group author members need to be listed in the author byline in order to be indexed, and if
749
included in the byline, must qualify for authorship according to our criteria.
750
- Read about the group authorship policy.
751
Author contributions
752 753
Provide at minimum one contribution for each author in the submission system. Use the CRediT
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taxonomy to describe each contribution. Read the policy and the full list of roles.
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Contributions will be published with the final article, and they should accurately reflect
756
contributions to the work. The submitting author is responsible for completing this information at
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submission, and we expect that all authors will have reviewed, discussed, and agreed to their
758
individual contributions ahead of this time.
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PLOS ONE will contact all authors by email at submission to ensure that they are aware of the
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submission.
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Cover letter
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Upload a cover letter as a separate file in the online system. The length limit is 1 page. The cover
763
letter should include the following information:
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- Summarize the study’s contribution to the scientific literature
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- Relate the study to previously published work
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- Specify the type of article (for example, research article, systematic review, meta-analysis, clinical
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trial)
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- Describe any prior interactions with PLOS regarding the submitted manuscript
769
- Suggest appropriate Academic Editors to handle your manuscript (see the full list of Academic
770
Editors)
771
- List any opposed reviewers
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IMPORTANT: Do not include requests to reduce or waive publication fees in the cover letter. This
773
information will be entered separately in the online submission system.
Read about publication fee assistance.
775
Title page
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The title, authors, and affiliations should all be included on a title page as the first page of the
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manuscript file.
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Download our sample title, author list, and affiliations page (PDF)
779 780
Abstract
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The Abstract comes after the title page in the manuscript file. The abstract text is also entered in a
782
separat field in the submission system.
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The Abstract should:
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- Describe the main objective(s) of the study
785
- Explain how the study was done, including any model organisms used, without methodological
786
detail
787
- Summarize the most important results and their significance
788
- Not exceed 300 words
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Abstracts should not include:
790 - Citations 791 - Abbreviations, if possible 792 Introduction 793
The introduction should:
794
- Provide background that puts the manuscript into context and allows readers outside the field to
795
understand the purpose and significance of the study
796
- Define the problem addressed and why it is important
797
- Include a brief review of the key literature
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- Note any relevant controversies or disagreements in the field
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- Conclude with a brief statement of the overall aim of the work and a comment about whether that
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aim was achieved
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Materials and Methods
802
The Materials and Methods section should provide enough detail to allow suitably skilled
803
investigators to fully replicate your study. Specific information and/or protocols for new methods
804
should be included in detail. If materials, methods, and protocols are well established, authors may
805
cite articles where those protocols are described in detail, but the submission should include
806
sufficient information to be understood independent of these references.
807
Protocol documents for clinical trials, observational studies, and other non-laboratory investigations
808
may be uploaded as supporting information. Read the supporting information guidelines for
formatting instructions. We recommend depositing laboratory protocols at protocols.io. Read
810
detailed instructions for depositing and sharing your laboratory protocols.
811
Human or animal subjects and/or tissue or field sampling
812
Methods sections describing research using human or animal subjects and/or tissue or field
813
sampling must include required ethics statements. See the reporting guidelines for human research,
814
clinical trials, animal research, and observational and field studies for more information.
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Data
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PLOS journals require authors to make all data underlying the findings described in their
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manuscript fully available without restriction, with rare exception.
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Large data sets, including raw data, may be deposited in an appropriate public repository. See our
819
list of recommended repositories.
820
For smaller data sets and certain data types, authors may provide their data within supporting
821
information files accompanying the manuscript. Authors should take care to maximize the
822
accessibility and reusability of the data by selecting a file format from which data can be efficiently
823
extracted (for example, spreadsheets or flat files should be provided rather than PDFs when
824
providing tabulated data).
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For more information on how best to provide data, read our policy on data availability. PLOS does
826
not accept references to “data not shown.”
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Cell lines
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Methods sections describing research using cell lines must state the origin of the cell lines used. See
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the reporting guidelines for cell line research for more information.
830
Laboratory Protocols
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To enhance the reproducibility of your results, we recommend and encourage you to deposit
832
laboratory protocols in protocols.io, where protocols can be assigned their own persistent digital
833
object identifiers (DOIs).
834
To include a link to a protocol in your article:
835
1.Describe your step-by-step protocol on protocols.io
836
2.Select Get DOI to issue your protocol a persistent digital object identifier (DOI)
837
3.Include the DOI link in the Methods section of your manuscript using the following format
838
provided by protocols.io: http://dx.doi.org/10.17504/protocols.io.[PROTOCOL DOI]
839
At this stage, your protocol is only visible to those with the link. This allows editors and reviewers
840
to consult your protocol when evaluating the manuscript. You can make your protocols public at
841
any time by selecting Publish on the protocols.io site. Any referenced protocol(s) will automatically
842
be made public when your article is published.
843
New taxon names
Methods sections of manuscripts adding new taxon names to the literature must follow the reporting
845
guidelines below for a new zoological taxon, botanical taxon, or fungal taxon.
846
Results, Discussion, Conclusions
847
These sections may all be separate, or may be combined to create a mixed Results/Discussion
848
section (commonly labeled “Results and Discussion”) or a mixed Discussion/Conclusions section
849
(commonly labeled “Discussion”). These sections may be further divided into subsections, each
850
with a concise subheading, as appropriate. These sections have no word limit, but the language
851
should be clear and concise.
852
Together, these sections should describe the results of the experiments, the interpretation of these
853
results, and the conclusions that can be drawn.
854
Authors should explain how the results relate to the hypothesis presented as the basis of the study
855
and provide a succinct explanation of the implications of the findings, particularly in relation to
856
previous related studies and potential future directions for research.
857
PLOS ONE editorial decisions do not rely on perceived significance or impact, so authors should
858
avoid overstating their conclusions. See the PLOS ONE Criteria for Publication for more
859
information.
860
Acknowledgments
861
Those who contributed to the work but do not meet our authorship criteria should be listed in the
862
Acknowledgments with a description of the contribution.
863
Authors are responsible for ensuring that anyone named in the Acknowledgments agrees to be
864
named.
865
- Do not include funding sources in the Acknowledgments or anywhere else in the manuscript
866
file. Funding information should only be entered in the financial disclosure section of the
867
submission system.
868
References
869
Any and all available works can be cited in the reference list. Acceptable sources include:
870
- Published or accepted manuscripts
871
- Manuscripts on preprint servers, providing the manuscript has a citable DOI or arXiv URL. Read
872
the Preprint Policy.
873
Do not cite the following sources in the reference list:
874
- Unavailable and unpublished work, including manuscripts that have been submitted but not yet
875
accepted (e.g., “unpublished work,” “data not shown”). Instead, include those data as
876
supplementary material or deposit the data in a publicly available database.
877
- Personal communications (these should be supported by a letter from the relevant authors but not
878
included in the reference list)
References are listed at the end of the manuscript and numbered in the order that they appear in the
880
text. In the text, cite the reference number in square brackets (e.g., “We used the techniques
881
developed by our colleagues [19] to analyze the data”). PLOS uses the numbered citation (citation-
882
sequence) method and first six authors, et al.
883
Do not include citations in abstracts or author summaries.
884
Make sure the parts of the manuscript are in the correct order before ordering the citations.
885
Formatting references
886 887
- Because all references will be linked electronically as much as possible to the papers they
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cite, proper formatting of the references is crucial.
889
PLOS uses the reference style outlined by the International Committee of Medical Journal Editors
890
(ICMJE) also referred to as the “Vancouver” style. Example formats are listed below. Additional
891
examples are in the ICMJE sample references.
892
A reference management tool, EndNote, offers a current style file that can assist you with the
893
formatting of your references. If you have problems with any reference management program,
894
please contact the source company's technical support.
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Journal name abbreviations should be those found in the National Center for Biotechnology
896
Information (NCBI) databases.
897
Source Format
Published articles
Hou WR, Hou YL, Wu GF, Song Y, Su XL, Sun B, et al. cDNA, genomic sequence cloning and overexpression of ribosomal protein gene L9 (rpL9) of