Os fungos endofiticos têm mostrado um grande potencial na produção de metabolitos bioativos. Nos últimos 2 anos, mais de 250 novos metabolitos produzidos por fungos endofiticos foram identificado. A presente proposta de trabalho tem como objetivo geral avaliar o potencial antimicrobiano de fungos endofiticos isolados de folhas de oliveira, bem como identificar o efeito da planta hospedeira nesta propriedade. O perfil volátil das espécies fungicas será ainda avaliado com o intuito de correlacionar os compostos identificados com atividade antimicrobiana exibida pelos endófitos. As espécies fúngicas a estudar serão: Penicillium commune, Penicillium canescens,
Alternaria alternata.
Objetivos especificos:
1- Avaliar a atividade antimicrobiana dos fungos endofíticos com crescimento em meio de cultura com e sem extratos aquoso de folhas de oliveira.
2- Avaliar, nas espécies fúngicas que mostraram maior atividade antimicrobiana, a Concentração Mínima Inibitória (CMI) de extratos fúngicos e de meio de cultura obtidos a partir de solventes orgânicos com diferentes polaridades. Nesta análise pretende-se avaliar se a produção dos compostos antimicrobianos, se encontram intercelularmente no micélio ou se são excretados para o meio de cultura.
3- Identificar, nas espécies fúngicas com menor CMI, os compostos volatéis potencialmente responsáveis pela atividade antimicrobiana, recorrendo a análise GC/MS.
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