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8 – Anexo
Tabela 11: Meio WC (Guillard & Lorenzen, 1972).
Nutrientes Concentração (mg/L) Macronutrientes CaCl2 .2H2O 36,76 MgSO4 .7H2O 39,97 KH2PO4 8,71 NaSiO3 .9H2O 28,42 FeCl3 .6H2O 3,15 NaHCO3 12,60 fonte nitrogenada* Micronutrientes Na2EDTA 4,36 CuSO4 .5H2O 0,01 ZnSO4 .7H2O 0,022 CoCl2 .6H2O 0,01 MnCl2 .4H2O 0,18 Na2MO4 .2H2O 0,006 H3BO3 1,00 NiSO4 . 6H2O** 0,198 Vitaminas Tiamina 0,10 Biotina 0,0005 Cianocobalamina 0,0005 *Fonte nitrogenada NaNO3 85,01 NH4CL 27,00 C6H14N4O2 43,50 C2H5NO2 75,00 NH2CONH2 30,00
(*) apenas uma das fontes utilizada em cada tratamento. (**) apenas adicionado em um dos tratamentos (ver texto).