SUGESTÕES PARA TRABALHOS FUTUROS

Sugere-se inicialmente como trabalho futuro uma análise mais aprofundada do efeito da influência do pH na produção dos biocombustíveis. Usualmente utiliza-se um pH médio de 7±0,10, mas há relatos na literatura de que outras algas verdes unicelulares como a Chlorella e Spirulina requerem pH alcalinos, em torno de 10, para que haja o crescimento da biomassa (KOTHARI et al., 2012). Neste contexto, a identificação de uma faixa de pH pode ser investigada em relação a produção de H2. Além de fornecer outras informações com relação ao

comportamento metabólico das algas para a síntese de inúmeros produtos do metabolismo. A avaliação do efeito de nutrientes como fosfato e traços de metais para a produção de H2 é recomendada. Alguns poucos estudos mostraram que o fosfato influência positivamente

na produção de hidrogênio (SUALI e SARBATLY, 2012). Enquanto que os traços de metais (ver Tabela 3.3) podem influenciar diretamente na atividade da enzima responsável pela evolução do hidrogênio através do sistema fermentativo no qual algas verdes são utilizadas. Uma vez que é sabido que alguns destes metais, como o ferro, tem a função de cofator na ativação de enzimas ([FeFe]-hidrogenase).

Reavaliar o sistema híbrido e co-cultura utilizado meio de fermentação com concentrações de enxofre de 10 mg de SO42- e os melhores resultados do planejamento fatorial

23 _{com fontes de carbono.}

Por fim, sugere-se ainda como atividade futura a avaliação da adição de outras fontes de carbono que possam potencializar a síntese isolada ou simultânea dos biocombustíveis avaliados neste estudo. Além da investigação de outras configurações de sistemas fermentativos como reatores de coluna de bolhas ou tanque agitado.

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