001. Adelino Vicente Mendonca Canario (Cat.: Professor Catedrático, Gr. Acad.: Agregação) 002. Deborah Mary Power (Cat.: Professor Catedrático, Gr. Acad.: Agregação)
003. Eduardo Nuno Picoto Lopes Barata (Cat.: Professor Auxiliar, Gr. Acad.: Doutoramento)
004. Teresa Isabel Mendonca Modesto (Cat.: Professor Auxiliar, Gr. Acad.: Doutoramento)
005. Maria Dulce da Mota Antunes de Oliveira Estêvão (Cat.: Professor-Adjunto, Gr. Acad.:
Doutoramento)
006. João Carlos dos Reis Cardoso (Cat.: Investigador Auxiliar, Gr. Acad.: Doutoramento) 007. Peter Colin Hubbard (Cat.: Investigador Auxiliar, Gr. Acad.: Doutoramento)
008. Christophe Haond (Cat.: Não aplicável (bolseiro), Gr. Acad.: Doutoramento)
010. Maria Begona Redruello Trelles (Cat.: Não aplicável (bolseiro), Gr. Acad.: Doutoramento) 011. Maria del Mar Huertas Pau (Cat.: Não aplicável (bolseiro) Gr. Acad.: Doutoramento )
012. Patrícia Isabel Silvestre Pinto (Cat.: Não aplicável (bolseiro), Gr. Acad.: Doutoramento)
013. Vanessa Schein (Cat.: Não aplicável (bolseiro), Gr. Acad.: Doutoramento)
2. Other researchers in the group (Include here collaborators with PhD. only) 001. Josep Rotllant (Cat.: Investigador Auxiliar Gr. Acad.: Doutoramento)
3. Other researchers in the group (non PhD.)
001. Ana Cristina da Silva Gomes (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
002. Ângela Alexandra Martinho Ramos (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
003. Bruno Emanuel Pereira Louro (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura) 004. Cristina Maria Simões de Jesus Rocha (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
005. Elsa Maria (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
006. Helena Rita de Carvalho Ferraz Pedrosa Teodósio (Cat.: Não aplicável (bolseiro), Gr. Acad.:
Licenciatura)
007. Isabel Maria Sena Morgado (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
008. Liliana Isabel Tome Dos Anjos Guerreiro (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura) 009. Marco António do Nascimento Sequeira de Jesus Campinho (Cat.: Não aplicável (bolseiro), Gr.
Acad.: Licenciatura)
010. Nádia Margarida Rosário Silva (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
011. Natalia Paola Moncaut (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
012. Nikolay Kolmakov (Cat.: Não aplicável (bolseiro), Gr. Acad.: Mestrado)
013. Olinda Gomes de Almeida (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
014. Pedro Luís Martins de Castro Pinheiro (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
015. Rita Alves Costa (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
016. Rute Sofia Tavares Martins Brazona (Cat.: Não aplicável (bolseiro), Gr. Acad.: Licenciatura)
6c. Objectives & achievements 1. Objectives (2000 ca.)
The main aim of the group is study the role of hormones and their metabolites on physiology and the evolution of the endocrine system, using fish as the principal model together with other key invertebrate and vertebrate models. The research follows four main interrelated themes which have developed from earlier work: 1) Hormonal control of Reproduction and Development; 2) Calcitropic hormones, mineral metabolism and environmental adaptation, 3) Hormones, pheromones and behavior, 4) The impact of Genotype on phenotype. Specific objectives are:
Hormonal control of Reproduction and Development
a) To establish the identity, function and mechanisms of action of key regulatory factors in relation to sex differentiation, puberty and gonad maturation.
b) To understand the process of metamorphosis and skeletal development in fishes, and the role of thyroid and metabolic hormones.
Calcitropic hormones, mineral metabolism and environmental adaptation
c) To understand the evolution of mineral homeostasis and the role of endocrine regulating factors, their site of action and the molecular mechanisms responsible for their biological actions.
d) To understand the process of ossification and regeneration in fishes and the role of the extracellular matrix, minerals and endocrine factors.
Hormones, pheromones and behavior
e) To understand the underlying mechanisms regulating the interaction between hormones and behaviour in fishes.
f) To understand how fish detect biologically important environmental chemical cues - pheromones, food-related odorants and inorganic cations (Ca2+ and Na+) and how this olfactory input is centrally processed to evoke the appropriate physiological and behavioural responses.
The impact of Genotype on phenotype
g) To apply an integrative and systems approach, from the genome to individuals and populations, to the study of the evolution of endocrine hormones/systems and their impact on phenotype.
2. Main achievements (2000 ca.) Scientific discoveries:
1) Established that in fishes the parathyroid family is composed of 5 peptides originating from different genes, three of which are present in all vertebrates. The target organs and calciotropic activity of the members of this family has been characterised.
2) Identified parathyroid hormone-related protein responsive genes and that this peptide mediates hypercalcemia during vitellogenesis and influences intestinal calcium fluxes, cortisol production in the interrenal and scale metabolism.
3) Proposed evolutionary model of family 2 G protein coupled receptors and established that 5 genes encode gonadotrophin releasing hormone receptors in fish.
4) Identified novel estrogen signaling molecules in fish testis and their transcriptional response to estrogen receptor agonists and antagonists.
5) Discovered a new aquaporin which controls egg hydration and floatability.
6) Discovered that chemical signalling of dominance status occurs via controlled urination in tilapia and other freshwater fish
7) Established in tilapia that unlike in dyadic interactions, fighting against its own image in a mirror does not modify endogenous androgens.
8) Established that androgens control the development of an external gland (anal gland) in male blennies, which produces a species-specific pheromone which attracts spawning females and enhance male reproductive success.
9) Established olfactory sensitivity to Ca2+ and Na+ as a real biological phenomenon in fish possibly related to rapid environmental adjustments.
Technology development /transfer:
10) Contributed to European aquaculture genetics and breeding through consortia which generated genomics resources for sea bass and sea bream: cDNA and BAC libraries, EST and genome sequences, microarrays, linkage and radiation hybrid maps.
11) Produced a dozen fish specific recombinant proteins as well as antisera and immunoassays for newly discovered peptides proteins, including parathyroid hormones and transthyretin.
12) Introduced transcriptome analysis technologies: SuperSAGE (I. Matsumura, Iwate Biotechnology Research Center, Japan); cell transfection, RNAi and transgenics (J Rotllant, Baltimore Biotechnology Centre).
6d. Productivity
1. Publications in peer review journals (3000 ca.) (Up to a max of 10. Always indicate at the end of the citation, impact factor of the journal (IF=) and number of citations (nº C=).
Give title and full citation in original language. DO NOT translate)
Between 2003-2006 the CME group published 96 SCI articles, of which 4 reviews: 24 in journals of IF>3;
1. Barata, EN, Hubbard, PC, Almeida, OG, Miranda, A, Canario, AVM. 2007. Male urine signals social rank in the Mozambique tilapia (Oreochromis mossambicus). Bmc Biology 5: 54. IF
2. Oliveira, RF, Carneiro, LA, Canario, AVM. 2005. No hormonal response in tied fights. Nature 437: 207-208. IF 2007=28.751, nºC=12.
3. Redruello, B, Estevao, MD, Rotllant, J, Guerreiro, PM, Anjos, LI, Canario, AVM, Power, DM.
2005. Isolation and characterization of piscine osteonectin and downregulation of its expression by PTH-related protein. Journal of Bone and Mineral Research 20: 682-692. IF 2007=6.004, nºC=9.
4. Rotllant, J, Guerreiro, PM, Anjos, L, Redruello, B, Canario, AVM, Power, DM. 2005.
Stimulation of cortisol release by the N terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro. Endocrinology 146: 71-76. IF 2007=5.045, nºC=14.
5. Fuentes, J, Figueiredo, J, Power, DM, Canario, AVM. 2006. Parathyroid hormone-related protein regulates intestinal calcium transport in sea bream (Sparus auratus). American Journal of Physiology-Regulatory Integrative and Comparative Physiology 291: R1499-R1506. IF 2007=3.661, nºC=6.
6. Canario, AVM, Rotllant, J, Fuentes, J, Guerreiro, PM, Teodosio, HR, Power, DM, Clark, MS.
2006. Novel bioactive parathyroid hormone and related peptides in teleost fish. Febs Letters 580:
291-299. IF 2007=3.263, nºC=7.
7. Santos, CRA, Estevao, MD, Fuentes, J, Cardoso, JCR, Fabra, A, Passos, AL, Detmers, FJ, Deen, PMT, Cerda, J, Power, DM. 2004. Isolation of a novel aquaglyceroporin from a marine teleost (Sparus auratus): function and tissue distribution. Journal of Experimental Biology 207:
1217-1227.IF 2007=2.972, nºC=12.
8. Fabra, M, Raldua, D, Power, DM, Deen, PMT, Cerda, J. 2005. Marine fish egg hydration is aquaporin-mediated. Science 307: 545-545. IF 2007=26.372, nºC=15.
9. Cardoso, JCR, Pinto, VC, Vieira, FA, Clark, MS, Power, DM. 2006. Evolution of secretin family GPCR members in the metazoa. Bmc Evolutionary Biology 6: 108. IF 2007=4.091, nºC=7.
10. Cavaco, JEB, Santos, CRA, Ingleton, PM, Canario, AVM, Power, DM. 2003. Quantification of prolactin (PRL) and PRL receptor messenger RNA in gilthead Seabream (Sparus aurata) after treatment with Estradiol-17 beta. Biology of Reproduction 68: 588-594. IF 2007=3.670, nºC=14.
2. Other publications national (3000 ca.) (Include only Books, chapters or full papers published in conference proceedings up to max of 10. Give title and full citation in original language)
1. Louro, BEP, Passos, ALS, Power, DM. 2006. Transcriptome Analysis of the gilthead sea bream (Sparus auratus) Pituitary gland: type I markers for molecular genetics. Revista Portuguesa Zootecnia, XII (II): 91-104.
3. Other publications international (3000 ca.) (Include only Books, chapters or full papers published in conference proceedings up to max of 10. Give title and full citation in original language)
Between 2003-2006 the CME group published 19 book chapters/proceedings internationally:
1. Ingleton, PM, Power, DM. 2004. Evolution of Prolactin. In: Luciano, M (ed) Encyclopedia of Endocrine Diseases. New York: Elsevier, pp. 104-110. ISBN 978-0-12-475570-3.
2. Canário, AVM, Fuentes, J, Guerreiro, PM, Power, DM. 2007. Parathyroid Hormone and Related Peptides in Fish: From Sequence to Function. In: Novel Aspects of PTHrP Physiopathology. Luparello, C (Ed.), pp. 27-40. Nova Publishers, ISBN:1-60021-857-1.
Guerreiro, PM, Fuentes, J. 2007. Control of calcium balance in fish. In: Fish Osmoregulation. B Baldisserotto, JM Mancera, BG Kapoor (Eds.), pp. 427-495, Science Publisher, Inc., USA, ISBN 978-1-57808-447-0/ 2007.
3. Mancera, JM, Fuentes, J. 2006. Osmoregulatory action of hypophyseal hormones in Teleosts.
In: Fish Endocrinology. BG Kapoor, G Zaccone, M Reinecke (Eds), pp. 393-417. Science Publishers, Inc. Enfield (NH). USA; Plymouth, U.K. & Oxford & IBH Publishing Co. Pvt. Ltd., ISBN 1-57808-415-6.
4. Redruello, B, Rotllant, J, Guerreiro, PM, Estêvão, D, Anjos, L, Power, DM, Canário, AVM.
2003. Osteonectin from sea bream: phylogeny, ontogeny and hormonal regulation. In: Avances en Endocrinlogía Comparada, vol. II. JP Castaño, MM Malagon, S Garcia-Navarro (Eds), pp.
125-128. Universidad de Córdoba: Córdoba, España.
5. Rotllant, J, Fernandes, H, Anjos, L, Guerreiro, PM, Redruello, B, Fuentes, J, Canário, AVM, Power, DM. 2003. Studies on truncated forms of the (1-34) N-terminal peptide of parathyroid hormone-related protein defines regions important for activity. In: Avances en Endocrinlogía Comparada, vol. II. JP Castaño, MM Malagon, S Garcia-Navarro (Eds), pp. 107-112. Uni.
Córdoba: Córdoba, España.
6. Guerreiro, PM, Canário, AVM, Fuentes, J, Rotllant, J, Redruello, B, Power, DM, Renfro, JL.
2003. Possible actions of PTHrP on calcium and phosphate transport by winter flounder (Pseudopleuronectes americanus) renal proximal tubule cells. . En “Avances en Endocrinlogía Comparada” vol. II. JP Castaño, MM Malagon, S Garcia-Navarro (eds). pp. 113-117.
Universidad de Córdoba: Córdoba, España.
7. Campinho, MA, Silva, N, Sweeney, GE, Power, DM. 2007. Molecular, histological and morphological changes in skin of halibut during metamorphosis. In: Avanços em Endocrinologia Comparativa, Vol III. Canário AVM and Power DM. (Eds), pp. 155-158. Univ. Algarve, Faro.
8. Silva, N, Baptista, VM, Power, DM. 2007. Expression patterns of the main MLC isoforms during halibut (Hipoglossus hipoglossus L.) metamorphosis. In: Avanços em Endocrinologia Comparativa. Vol III, Canário AVM and Power DM (Eds), pp. 165-168. Univ. Algarve, Faro.
9. Louro, B, Power, DM. 2007. Identification of polymorphisms in endocrine related transcripts in the sea bream (Sparus auratus). In: Avanços em Endocrinologia Comparativa, Vol III. Canário AVM and Power DM (Eds), pp. 187-190. Univ. Algarve, Faro.
10. Cardoso, J, de Vet, E, Elgar, G, Power, DM. 2007. Functional Characterization of PACAP receptors from sea bream, Sparus auratus. In: Avanços em Endocrinologia Comparativa, Vol III.
Canário AVM and Power DM (Eds), pp. 79-82. Univ. Algarve, Faro.
4. Master and Ph.D. thesis completed (3000 ca.) PhD THESIS
Serrano, RM. 2008. Pheromones in the reproduction of Salaria pavo and S. fluviatilis (Pisces:
Blenniidae): a comparative study. University of Évora. Co-supervisors: EN Barata, PC Hubbard.
Brinca, LF. 2007. Characterization of prolactin from Sparus aurata L. and the mechanisms controlling its synthesis and secretion. Univ. Algarve, 2007. Supervisor: DM Power.
Campinho, M. 2007. The molecular and endocrine basis of finfish embryo development and metamorphosis. University of Wales Cardiff, UK. Co-supervisors: DM Power and Glen Sweeney (Univ Cardiff).
Cruz, M. 2007. Ciclo Hormonal Reprodutor, Clonagem e Expressão da Enzima P450 Aromatase na Tilápia, Oreochromis mossambicus (Teleostei: Cichlidae). University of Algarve. Supervisor:
AVM Canário.
Estêvão, MD. 2007. Comparative study of hormonal regulation of calcium in vertebrates.
University of Algarve. Supervisor: DM Power.
Ramos, P. 2007. Contribution to the study of thyroid hyperplasia in pleuronectids. Technical Univ. of Lisbon. Co-supervisors: DM Power and Maria da Conceição Peleteiro (Tech. Univ Lisbon).
Morgado, I. 2007. The role of transthyretin in thyroid hormone transport in fishes and the effect of endocrine deregulators in this process. University of Algarve. Supervisor: DM Power.
Moncaut, N. 2006. Isolation and functional characterization of gonadotrophin releasing hormone receptors. ICBAS, University of Porto. Supervisor: AVM Canário.
Pinto, P. 2006. Diversity, expression and mechanism of action of estrogen receptors in seabream, Sparus aurata. University of Algarve. Supervisors: AVM Canário and Glen Sweeney (Cardiff University, UK).
Modesto, T. 2003. Hormonal control of the reproductive cycle of the Lusitanian Toadfish, Halobatrachus didactylus. University of Algarve. Supervisor: AVM Canário.
MSc THESIS (out of >10)
Diogo, ML. 2003. Role of the interrenal in temperature-induced sex differentiation in tilapia, Oreochromis mossambicus. MSc Aquaculture, University of Algarve. Supervisor: AVM Canário.
Martins, R. 2003. Does the interrenal influence sex differentiation in sea bass, Dicentrarchus labrax? MSc Aquaculture, University of Algarve. Supervisor: AVM Canário.
Oléjua, MAP. 2007. Effects of the goitrogenic compounds methimazole, thiourea and propylthiouracil on the fish thyroid axis, in vivo: the sea bream (Sparus auratus) model. MSc Marine Biology, University of Algarve. Supervisors: AVM Canário, P Pinto.