CURRICULUM VITAE EDUARDO PINHO E MELO

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CURRICULUM VITAE EDUARDO PINHO E MELO

1. GENERAL

Name: Eduardo José Xavier Rodrigues de Pinho e Melo Date of Birth: April 17^th, 1964

Nationality: Portuguese

Address: Av. do Parque, aldeamento Vila Flor, nº 22, 8125-404 Quarteira, Portugal E-mail: emelo@ualg.pt

2. UNIVERSITY DEGREES

B.Sc. in Biological Sciences, University of Lisbon, 1989 Ph.D. in Biotechnology, Instituto Superior Técnico, 1995 Aggregation in Biotechnology, Instituto Superior Técnico, 2007

3. PROFESSIONAL HISTORY

2003-present Associate Professor, University of Algarve, Portugal

1995-present Head of the Protein Biochemistry and Biophysics Lab, Centre of Marine Sciences, University of Algarve, Portugal

2020-2023 Steering Committee, PhD in Biological Sciences, University of Algarve, Portugal

2019-2022 Steering Committee, BSc and Master in Biotechnology, University of Algarve, Portugal

2019 (June-July) Visiting Professor, UK Dementia Research Institute (Edward Avezov lab), University of Cambridge, UK

2017-2018 Sabbatical leave, Cambridge Institute for Medical Research (David Ron Lab), University of Cambridge, UK

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2016-2018 Vice-director of the Centre for Biomedical Research (CBMR), Univ.

of Algarve

2016 (June-July) Visiting Professor, Cambridge Institute for Medical Research (David Ron lab), University of Cambridge, UK

2013 (June-July) Visiting Professor, Institute of Metabolic Sciences (David Ron lab), University of Cambridge, UK

2012 (June-July) Visiting Professor, Institute of Metabolic Sciences (David Ron lab), University of Cambridge, UK

2013-2015 Director of BSc in Biochemistry, University of Algarve, Portugal 2011-2013 Director of the Master in Biological Engineering, University of

Algarve, Portugal

2010 Visiting Professor, New York Univ. School of Medicine, Skirball Institute of Biomolecular Medicine (David Ron lab), USA

2007 Aggregation in Biotechnology, Instituto Superior Técnico, University of Algarve, Portugal

1995-2003 Assistant Professor, Universidade of Algarve, Portugal

2003 (Feb-Aug) Visiting Professor, Institute of Neuropathology (Adriano Aguzzi lab), University Hospital of Zurich, Switzerland

2000 (Oct-Nov) Visiting Professor, Aalborg University, Denmark

1998 (August) Visiting Researcher, Protein Engineering Lab. (Steffen Petersen lab), Department Biotechnology, University of Aalborg, Denmark

1998 Visiting Researcher, Lab. of Cell Physiology & NMR (Helena Santos lab), Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal

1992-1993 Visiting Researcher, Lab. of Lipolyse Enzymatique (Robert Verger lab), Centre National Recherche Scientifique (CNRS), Marselha, France

1991-1995 Ph.D. Student, Biological Engineering Research Group, Instituto Superior Técnico, Portugal, (Supervisors: J. Sampaio Cabral and Sílvia Brito Costa)

1990-1991 M.Sc. Student in Biotechnology, Instituto Superior Técnico, Portugal (Credits full filled)

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4. TEACHING ACTIVITIES

2019 PhD Program in Molecular Biosciences (MolBiolS) from Instituto de Tecnologia Química e Biológica (ITQB), New University of Lisbon.

Topic: “Live-cell imaging of fluorescent probes track oxidative stress and protein aggregation in the endoplasmic reticulum”

Topics: “Prions as infectious diseases” and “The Yin and Yang of protein aggregation”

Topics: “Protein oxidative folding and proteotoxicity” and “Prions as infectious diseases”

2014-2015 PhD Program in Mechanisms of Diseases and Regenerative Medicine (Pro-RegeM) a joint collaboration between University of Algarve and New University of Lisbon. Topic: Prion Diseases

2013-2015 Director of the BSc in Biochemistry, University of Algarve 2012-2015 Coordinator of the Biochemistry area, University of Algarve

2011-2013 Director of the Master in Biological Engineering, University of Algarve 2012-2016 Responsible for the units: Structure and Function of Proteins, and

Proteomics from the Master in Biological Engineering, and Techniques in Molecular and Cell Biology from the Master in Marine Biology and Fisheries and Aquaculture, University of Algarve. Teacher at the units Functional Genomics and Structural Biochemistry from the BSc in Biochemistry.

2012-2015 Responsible for the units: Structure and Function of Proteins, and Proteomics from the Master in Biological Engineering, and Techniques in Molecular and Cell Biology from the Masters in Marine Biology and Fisheries and Aquaculture, University of Algarve.

2010-2012 Responsible for the units: Enzyme Engineering, Structure and Function of Proteins, Recovery of Biological Products and Proteomics from the Master in Biological Engineering, and Techniques in Molecular and Cell Biology from the Masters in Marine Biology and Fisheries and Aquaculture, University of Algarve

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2007-2008 Responsible for the Enzyme Engineering, Structure and Function of Proteins and Proteomics units from the Master in Biological Engineering, University of Algarve

2007-2008 Responsible for the Physical Biochemistry unit from the BSc in Biochemistry, University of Algarve

1996-2007 Responsible for the Downstream I and Enzyme Technology units from the BSc in Biological Engineering, University of Algarve

2005-2006 Responsible for the Function and Structure of Proteins unit of the Master in Biotechnology, University of Algarve

2000-2001 Teacher of the Enzyme Production unit of the degree in Food Engeneering, University of Algarve

1998-2000 Lecture on Folding and Stability of Proteins of the Master in Biotechnology, Instituto Superior Técnico

1996-1999 Responsible for the Downstream II unit of the degree in Biological Engineering, University of Algarve

1997-1998 Teacher of practical classes on Biochemistry and Methods on Analytical Chemistry of the degree in Biological Engineering, University of Algarve 4.1. Thesis Supervisions

Supervision of Post-Doctoral studies:

2010-2016 Joana Macedo, “Function and Infectivity of Prions during Neural Differentiation of Embryonic Stem Cells”, Universidade do Algarve (supervisor)

2006-2007 Chen LuYang, “Protein Folding and Stabilization by Molecular and Chemical Chaperones”, Instituto Superior Técnico (supervisor)

2005-2008 Catarina Madeira, “A Murine Neuroblastoma Cell-Based Approach to Study and Prevent Prion Diseases”, Instituto Superior Técnico (supervisor)

Supervision of Ph.D. thesis:

2012-2016 PhD in Chemical Engineering Mónica Rosa, “Development of Technology for cryopreservation and formulation of therapeutic proteins”, Instituto Superior Técnico (co-supervisor)

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2009-2013 PhD in Biological Sciences: Evandro Tavares, “An Animal Cell-Based Approach to Study Prion Diseases: Cellular Trafficking, Conformational Changes and Prion Infectivity”, Universidade do Algarve (supervisor) 2005-2011 PhD in Biological Sciences: Nídia Estrela, “Prion-Diseases: A Biophysical

approach to the pathological protein conformer” Universidade do Algarve (supervisor)

2005-2009 PhD in Biochemistry: André T. Fernandes, “Structure-Function relationships and stability of extremophilic lacases” Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (co-supervisor)

2003-2007 Ph.D. in Biotechnology: Ricardo Vidal Pimenta Baptista, “Protein Stabilization by media engineering and directed evolution”, Instituto Superior Técnico (supervisor)

2001-2005 Ph.D. in Biotechnology: Chen Luyang, “Folding and Stability of Proteins in the presence of Low Molecular Weight Solutes”, Instituto Superior Técnico (supervisor)

2000-2004 Ph.D. in Biotechnology: Ana Sofia Leitão Carvalho, “Conformational Stability Studies of Two Anionic Horseradish Peroxidases Isoenzymes”, Instituto Superior Técnico (co-supervisor)

Supervision of Master thesis:

2019 Master in Biological Engineering: Joana Silva, “Estudos de refolding oxidativo de proteínas”, University of Algarve, Portugal

2013 Master in Biological Engineering: Fernando Rodrigues, “Catálise de pontes dissulfureto pelas vias enzimáticas que actuam no retículo endoplasmático usando como substrato a proteína fluorescente HyPer”, University of Algarve, Portugal

2013 Master in Biological Engineering: Jaqueline Custódio, “Neurotoxicidade de fibrilhas da proteína priónica”, University of Algarve, Portugal

2012 Master in Biological Engineering: Cátia Pires, “Fibrillation and neurotoxicity of the prion protein”, University of Algarve, Portugal

2012 Master in Biological Engineering: Inês Teixeira, “Oxidative protein folding in the endoplasmic reticulum”, University of Algarve, Portugal

2009 Master in Biological Engineering: Carlos Lopes, “Prião Murinico: Produção, separação, mutagenese e estabilidade à autólise”, University of Algarve, Portugal

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2001 Master in Biotechnology: Charlotte Hejl, “Improving thermal/structural stability of cutinase in the presence of low molecular weight solutes and by directed evolution”, Aalborg University, Denmark

Supervision of Undergraduate thesis:

2017 B.Sc. in Biomedical Sciences: Mariana Barbosa dos Santos, “The isomerase activity of protein disulfide isomerase”, University of Algarve

2011 B.Sc. in Biomedical Sciences: João Diogo Almeida, “Folding oxidativo de proteínas no Retículo Endoplasmático”, University of Algarve

2007 B.Sc. in Biotechnology: Evandro Tavares, “A cell-based infectivity assay for mouse prions”, University of Algarve

2007 B.Sc. in Biotechnology: Carlos Lopes, “Expression and purification of recombinant mouse prion protein”, University of Algarve

2004 B.Sc. in Biotechnology: Nídia Estrela, “Biophysical studies on prion proteins”, University of Algarve

2003 B.Sc. in Biotechnology: Luísa Pedro, “Thermodynamic stability of thermophilic and mesophilic ribosomal protein S6”, University of Algarve 2001 B.Sc. in Biochemistry: Claire L. Middleton, “A comparison of the

chemically induced unfolding patterns of the wild-type ribosomal protein S6 and point mutants of S6”, University of St. Andrews, Scotland

2000 B.Sc. in Biotechnology: Ricardo Vidal Pimenta Baptista, “Mechanisms of cutinase stabilization by trehalose”, University of Algarve

2000 B.Sc. in Biotechnology: João Miguel Faria Raposo Encarnação, “Biosorption of heavy metals”, University of Algarve

4.2. Examinations

2020 Diana Catarina Santana Gomes, “Understanding and improving the stability of therapeutic proteins in cold and frozen solutions”, PhD thesis in Chemistry, Instituto Superior Técnico, Portugal

2019 Odete Sofia Lopes Gonçalves, “Functional and structural effects of UV light on biomacromolecules”, PhD thesis, Faculty of Medicine, Aalborg University, Denmark

2019 Ana Elisabete Fernandes, “Towards cooperative organometalic and Enzymatic catalysis to develop new value chains from organic waste”, PhD in Chemistry, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa

2016 Lúcia Fátima Garcia Dias, “Amavadina e homólogos como promotores de aplicações tecnológicas”, Master in Chemical Engineering, Instituto Superior Técnico, Universidade de Lisboa

2016 Mónica Filipa da Silva Rosa, “Development of technology for cryopreservation and formulation of therapeutic proteins”, PhD in Chemical Engineering, Instituto Superior Técnico, Universidade de Lisboa

2015 Ana Rita Fragoso Nascimento, “Recombinant proteins in differentiation of stem cells”, Master in Biomedical Sciences, Universidade do Algarve

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2015 Fábio Miguel Vicente Anastácio, “Estudo do papel desempenhado pela proteína anexina A2 na regulação de vias de transdução de sinal activadas por Ras”, Master in Molecular and Microbial Biology, Universidade do Algarve

2015 Joaquim Paulo Curre Madeira, “Bacterial Hydrogen Peroxide forming enzymes”, Master in Biological Engineering, Universidade do Algarve 2014 Leonardo Mendes Silva, “Screening of process conditions for the production

of biogas from diluted organic waste streams using microreactors”, Master in Biological Engineering, Universidade do Algarve

2011 Ana Catarina Parreira Peres Matias, “Dissection of the early steps in the assembly of the 20S proteosome in Saccharomyces cerevisiae”, PhD in Biochemistry, Universidade do Algarve

2011 Liliana Isabel Tomé dos Anjos Guerreiro, “Biochemical and biological characteristics of novel piscine forms of Parathyroid Hormone and Parathyroid Hormone related Peptide: A proteomic approcah”, PhD in Biology, Universidade do Algarve

2010 Ana Sofia Fraga, “Conformational and functional alterations on an aspartic proteinase promoted by trifluoroethanol”, PhD in Biochemistry, Faculdade de Ciências e Tecnologia, Universidade de Coimbra.

2008 Sónia Cristina Alves Dickson Leal Solano, “Protein Folding, metal ions and conformational states: The case of a di-cluster ferredoxin”, PhD in Biochemistry, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa.

2008 Maria Teresa Gonçalves de Macedo Matamá, “Enzymatic treatement of acrylic and cellulose acetate fibres”, PhD in Textile Enginnering, Universidade do Minho.

2008 Patrícia da Silva Guerreiro, “Parkinson’s Disease: Substrate proteins and E2 enzymes involved in Parkin pathway”, Master in Biotechnology, Universidade do Algarve.

2007 Pedro Vidinha, “Strategies for the enhancement of the catalytic performance of cutinase in nonaqueous media”, PhD in Chemical Engineering, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa.

2007 Anabela de Oliveira Pereira, “Cardosina A como modelo para o estudo da estabilidade conformacional de proteinases aspàrticas”, PhD in Biology, Universidade de Aveiro.

2007 Teresa Margarida Pedrosa Cardoso, “Conformational Stability of the Archetypal Aspartic Proteinase, Pepsin A”, PhD in Biochemistry, Universidade de Aveiro.

2007 Isabel Maria Sena Morgado, “Transthyretin and thyroid hormone transport in fish and the effect of endocrine disruptors on this process”, PhD in Biology, Faculdade de Ciências do Mar e do Ambiente, Universidade do Algarve.

2006 Vânia Sofia Soares Pereira Brissos, “In vitro evolution of lipolytic enzymes.

Stabilization of cutinase against surfactants”, PhD in Biotecnologia, Instituto Superior Técnico, Universidade Técnica de Lisboa.

2006 Teresa Isabel Gonçalves Carvalho, “Transições Conformacionais da Proteína Bovina β-Lactoglobulina: Estudos em Equílibrio”, Master in Biotecnologia

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(Engenharia Bioquímica), Instituto Superior Técnico, Universidade Técnica de Lisboa.

2005 Sandra Cristina Pinto da Rocha, “Interface Controlled Secondary Structure Changes of the Fibril Forming Peptides B18 and β Amyloid”, PhD Thesis in Engineering Sciences, Universidade do Porto.

2004 Shona Pedersen, “Protein Stability, Folding and the Interaction with Ultra Violet Light”, PhD Thesis, Aalborg University, Denmark.

2004 Ana Sofia Leitão Carvalho, “Conformational Stability of two anionic Horseradish peroxidase isoenzymes”, PhD in Biotechnology, Instituto Superior Técnico, Universidade Técnica de Lisboa.

2003 Chen Zhenjia, “Morphocultural and Pathogenic Comparisons between Colletotrichum kahawae and Colletotrichum gloeosporioides isolated from Coffee Berries”, PhD in Engenharia Agronómica, Instituto Superior de Agronomia, Universidade Técnica de Lisboa.

2003 Daniela Cipreste Vaz, “Estabilidade Conformacional da Interleucina-4 Humana: Papel das Pontes de Enxofre”, Master in Biologia Celular, Faculdade de Ciências e Tecnologia, Universidade de Coimbra.

2002 Ana Maria Domingues Gonçalves, “Study of the Interactions between Reverse Micellar Components and Cutinase”, Ph.D. in Biotecnologia, Instituto Superior Técnico, Universidade Técnica de Lisboa.

2001 Patrícia Rodrigues Saavedra Leirião, “Derivatização de Suportes para Imobilização de Péptidos e Proteínas com diferentes Aplicações Biotecnológicas”, Master in Biotecnologia (Engenharia Bioquímica), Instituto Superior Técnico, Universidade Técnica de Lisboa.

2001 Fernando Sérgio Rodrigues Ribeiro Teles, “Desengorduramento de Resíduos da Indústria de Curtumes por Lipases”, Master in Biotecnologia (Engenharia Bioquímica), Instituto Superior Técnico, Universidade Técnica de Lisboa.

1999 Ana Margarida Nunes da Mata Pires de Azevedo, “Estudos de Estabilidade da Penicillina Acilase em Meio Acquoso e em Micelas Invertidas”, Master in Biotecnologia (Engenharia Bioquímica), Instituto Superior Técnico, Universidade Técnica de Lisboa.

5. SCIENTIFIC ACTIVITIES

5.1. Research Interests

My PhD degree obtained at Instituto Superior Técnico in Biotechnology has addressed structural-functional relationships of enzymes in non-conventional media such as reverse micelles. These low water content systems offer several advantages for the biotechnological exploitation of enzymes. Part of the PhD work was done at the lab. of Lipolyse Enzymatique from the Centre National Recherche Scientifique (CNRS) in Marselha, France. My interest on structure-function of proteins was then shifted to one of

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the most important issues in Biology: the folding of proteins. Protein folding and stability was since then one of the main focus of my research, using several proteins as models (the enzyme cutinase, the ribosomal protein S6 from Thermus thermophilus and multi-copper oxidases) and biophysical techniques as experimental tools. Studies on protein folding and stability were strengthened by leaves to the Aalborg University, Denmark, where collaborations with Steffen Petersen and Daniel Otzen were consolidated and fruitful. In 2003, a sabbatical leave to Adriano Aguzzi’s lab, at the Institute of Neuropathology, University Hospital of Zurich, was the opportunity to initiate work on prions. From this point, protein aggregation, in vitro and in cells, has become my main interest. Several neurodegenerative diseases, including Alzheimer and prion diseases, result from the misfolding of proteins that traffic through the endoplasmic reticulum (ER) to the cell membrane. One of the key features of protein folding in the endoplasmic reticulum is the formation of disulfide bonds, the so-called oxidative protein folding. Studies on oxidative protein folding and protein aggregation were pursuit in 2010 with a sabbatical leave to the lab of David Ron at the New York Univ., now affiliated to the Cambridge Institute for Medical Research, UK. More recently, in collaboration with Edward Avezov from the UK Dementia Research Institute, Cambridge University, we have been focusing on the effects of oxidative and thermal stress on protein aggregation in the ER. With my recent join to CCMAR I am planning to focus on proteostasis, i.e., the maintenance of protein folding and function in cells, and how stressors, such as ocean warming and acidification, may disturb cell homeostasis and accelerated proteostasis collapse resulting from protein misfolding and aggregation.

5.2. Invited Seminars

2017 E.P. Melo

"The Yin and Yang of Protein Aggregation"

Distinguished Seminars, Universidade do Algarve

2017 E.P. Melo

"Yin & Yang e Agregação de Proteínas: Dogmas da Biologia"

Universidade do Algarve

2017 E.P. Melo

"Priões: Proteínas Canibais"

Escola Secundária de Vila Real de Santo António, Vila Real de Santo António

2017 E.P. Melo

"Protein misfolding and Prion Diseases"

Instituto Superior Técnico, Universidade Técnica de Lisboa

2016 E.P. Melo

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2015 E.P. Melo

"Priões: Proteínas Canibais"

Escola Secundária Francisco Fernandes Lopes, Olhão

2015 E.P. Melo

2014 E.P. Melo

"Priôes: Reminiscências negras de um passado brilhante?"

Universidade do Algarve, IV Simpósio de Ciências Biomédicas 2014 E.P. Melo and L. O. Martins

"Insight into the stability of multi-copper oxidases"

University of Maribor, Slovenia

2014 E.P. Melo

2013 E.P. Melo

" Prion Diseases"

2012 E.P. Melo

" Protein fibrillation, osmolytes and prion diseases "

Faculdade de Ciências, Universidade de Lisboa, organizado pela Sociedade Portuguesa de Biofísica

2012 E.P. Melo

"Spots Fluorescentes no Centro de Química Estrutural"

Instituto Superior Técnico, Jubilação da Profª Sílvia Brito Costa

2011 E.P. Melo

"Protein Fibrillation, prion diseases and the origin of life"

Instituto Superior Técnico, Portugal, Master on Biomedical Engineering.

2010 E.P. Melo

"Prions"

2009 E.P. Melo

"Prions"

2009 E.P. Melo

"Protein Fibrillation and Prion Diseases"

Universidade da Beira Interior, Portugal, Degree on Medicine.

5.3. Oral Communications 2019 Melo, E. P.

“Stability of protein formulations at sub-zero temperatures by isochoric cooling”

Biological and pharmaceutical complex fluids III: Protein self-assembly, rheology and interfacial properties, Hernstein, Austria

2018 Melo, E. P., I. Farace, C. Correia, E. Tavares, X. Zhang, E. Avezov and D. Ron

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“Live-cell imaging of protein disaggregation in the endoplasmic reticulum”

7th Iberian congress on prions, Laguardia, Spain 2016 Melo, E. P.

“The Yin and Yang of Protein Aggregation”

ProtStab 2016, 11th International Conference on Protein Stabilisation, Istambul, Turkey

2015 Estrela, N., C. Lopes, J. A. Macedo, E. Tavares, H. G. Franquelim, G.

Christiansen, D. E. Otzen and E. P. Melo

“Getting proteins more compact prevents protein amyloidosis”

4^th Iberian Congress on Prions, Lisbon, Portugal

2015 Macedo, J. A., D. Schrama, P. M. Rodrigues, I. Duarte, E. Tavares, J.

Renaut and E. P. Melo

“Membrane-enriched proteomic changes associated with prion protein expression during neural differentiation and in neuroblastoma cells”

4^th Iberian Congress on Prions, Lisbon, Portugal

2014 J. A. Macedo, D. Schrama, P. M. Rodrigues, J. Renaut, E. Tavares and E. P. Melo

“Membrane proteome and prion protein profiling during neural differentiation of stem cells”

3^rd Iberian Congress on Prions, Zaragoza, Spain

2014 N. Estrela, C. Lopes, J. A. Macedo, E. Tavares, H. G. Franquelim, G.

Christiansen, D. E. Otzen, M. Rosa, V. Geraldes, M. A. Rodrigues, S. K.

Singh and E. P. Melo

“Osmolyte induce compaction of the protein tertiary structure preventing amyloid fiber formation and protein aggregation below the freezing temperature”

10^th International Conference on Protein Stabilization, Stresa, Italy 2014 M. Rosa, C. Lopes, E. P. Melo, S. K. Singh and M. A. Rodrigues

“Accelerated Formulation Studies for Frozen Storage of Proteins”

9^th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, Lisboa, Portugal”

2013 E. Tavares, J. A. Macedo, P. M. R. Paulo, C. Tavares, C. Lopes and E.

P. Melo

“Live-Cell imaging FRET reveals clustering of the prion protein at the cell surface induced by infectious prions”

2^nd Iberian Congress on Prions, Faro, Portugal

2013 V. Brissos, N. Gonçalves, A. M. Gonçalves, I. Bento, E. P. Melo and L.

O. Martins

“Stabilization of FMN-dependent NADPH:dye/quinone reductase from Pseudomonas putida MET94 by directed evolution”

9^th European Biophysical Congress, Lisboa, Portugal.

2012 E. Tavares, C. Tavares, J.A. Macedo and E.P. Melo

"Detection and Neurotoxicity of Prions"

1st Iberian Congress on Prions, Santiago de Compostela, Spain.

2012 E.P. Melo, N.L. Estrela, V. Ochoa-Mendes, C. Lopes, E. Tavares and J.

Macedo

"Protein fibrillation, osmolytes and prion diseases"

9^th International Conference on Protein Stabilization, Lisboa, Portugal.

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2011 E.P. Melo

"Global approach to proteomics"

Three days Proteomics course: From 2DE to protein identification by mass spectrometry, Algarve, Portugal.

2011 E.P. Melo

"Protein Fibrillation, Prion Diseases and the Origin of Life"

I Ciclo de Conferências – Núcleo de Estudantes de Ciências Biomédicas, Algarve, Portugal.

2010 E.P. Melo

"Folding kinetics and Stability"

Protein folding, misfolding and disease, 9^th short course of the Portuguese Biophysical society, Santarém, Portugal.

2010 A.T. Fernandes, E.P. Melo, L.O. Martins

"Stability of CotA – laccase from Bacillus subtilis and Metallo-Oxidase McoA from Aquifex aeolicus to thermal and chemical stability"

Oxidative Enzymes as Sustainable Industrial Biocatalysts, Santiago de Compostela, Spain.

2010 Fernandes, A.T., E. P. Melo, L. O. Martins

"Insight into the stability of multicopper oxidases”

Protein-Protein Interactions and Protein folding. FCT-UNL. Lisbon.

Journées Jeunes Chercheurs. Bourdeaux. France.

Oxizymes & 9th International Symposium of Peroxidases. Leipzig, Germany.

3rd Annual World Congress of Industrial Biotechnology – IBIO 2010.

Dalian, China.

2009 Estrela, N.L., L.Y. Chen, S.M. C. Gunna, J.M.S. Cabral, D.E. Otzen and E.P. Melo

"Protein Fibrillation is prevented by Osmolytes"

8^th International Conference on Protein Stabilisation, Graz University of Technology, Austria.

2008 Estrela, N.L., L.Y. Chen, S.M. C. Gunna, G.J.M. Cabrita, D.E. Otzen and E.P. Melo

"Folding and amyloidosis of proteins: The prevention of amyloidosis by osmolytes"

XVI^th National Congress of Biochemistry, Universidade dos Açores, Portugal

2008 Madeira, C., J.A.B. Ferreira, S.M. Andrade, G.J.M. Cabrita, S.M.B.

Costa and E.P. Melo

"Fluorescence studies of the pair CFP-YFP: FLIM-FRET in vitro studies and expression in neuronal cells to probe the prion protein at the cell surface"

Focus on Microscopy, Osaka University, Japan

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2008 Chen, Z., P. Durão, A. T. Fernandes, V. Brissos, L. Pereira, S.

Todorovic, M. M. Pereira, I. Bento, E. P. Melo, P. F. Lindley, L. O.

Martins

"Copper incorporation into recombinant CotA-laccase from Bacillus subtilis”

Oxizymes, 4th European meeting in Oxizymes. Helsinki, Finland.

2007 Melo, E.P.

"Proteins at the Cell Surface Probed by Fluorescence Lifetime Imaging Microscopy"

Portugal-MIT program on Bioengineering, Instituto Superior Técnico, Portugal

2007 Melo, E.P., A.T. Fernandes, L.O. Martins

"Insight Into the Stability of Multi-Copper Oxidases"

7^th International Conference on Protein Stabilization, University of Exeter, England

2006 Melo, E.P.

"Priões "

Forum da Química, Universidade Nova de Lisboa, Portugal 2006 Melo, E.P.

"Enzyme Stabilization: different strategies for different enzymes "

6^th European Symposiun on Biochemical Engineering Science, Salzburg, Austria

2006 Durão, P., I. Bento, A. T. Fernandes, E. P. Melo, P. F. Lindley, L. O.

Martins

“Perturbations of the T1 copper site in the CotA-laccase from Bacillus subtilis: Structural, Biochemical, Enzymatic and Stability Studies”

Eurobic8. 8th European Biological Inorganic Chemistry Conference.

Aveiro, Portugal.

2006 Fernandes, A.T., C. M. Soares, M. M. Pereira, R. Huber, G. Grass, E.

P. Melo, L. O. Martins

“Enzymatic properties, structural insights and stability of a highly efficient metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus”

Copper 06. 5th International Copper Meeting. Alghero, Sardinia-Italy.

2005 Melo, E.P., L. Chen, R. P. Baptista, G. J. M. Cabrita, D. E. Otzen

"Protein Folding and Stabilization by Osmolytes "

International Conference on Enzyme Technology, RELATENZ 2005, Cuba 2004 Luyang Chen, M. Mateus, D. Otzen, E.P. Melo

"Effect of Trehalose on Folding and Stability of the Ribosomal Protein S6 "

6th International Conference on Protein Stabilization, Bratislava, Slovakia

2001 E.P. Melo

"Cutinase Folding and Stabilization by Trehalose"

10th European Congress on Biotechnology, Madrid, Spain

1999 E.P. Melo

"Lipase interactions with Micelles"

Structure Function and Stability of Lipases. A Ph.D. School, Aalborg, Denmark

1997 E.P. Melo

"Deactivation Equilibria for Cutinase in Reverse Micelles"

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8th European Congress on Biotechnology, Budapeste, Hungary 5.4. Advisory PhD Committee

Diogo Silva, Microbial & Enzyme Technology Lab., supervisor Lígia Martins, ITQB, Univ. Nova, 2018-2021

Patrícia Manuela Areias da Silva, PhD in Biomedical Sciences, Univ. of Algarve, supervisor Hassan Bousbaa, IBMC, Instituto de Biologia Molecular e Celular, Univ.

do Porto, 2013-2017

Ana Sofia Lourenço, PhD in Biomedical Sciences, Univ. of Algarve, supervisor Inês Araújo, Univ. of Algarve, 2012-2016

5.5. Other scientific activities

Scientific Committee Member of the 9^th Iberian Congress on Prions, Corunha, Spain,2020 Chairman in the 7^th Iberian congress on prions, Laguardia, Spain, 2018.

Scientific Committee Member of the 7^th Iberian Congress on Prions, Laguardia, Spain, 2018

Organizing Committee Member of Prion 2018, Santiago de Compostela, Spain, 2018 Scientific Committee Member of 5^th Iberian Congress on Prions, Barcelona, Spain, 2016 Scientific Committee Member of 4^th Iberian Congress on Prions, Lisboa, Portugal, 2015 Head of the organizing committee of the 2^nd Iberian Congress on Prions, 2-3 December, 2013, University of Algarve, Faro

Chairman of the “Protein Folding” session in the 9^th European Biophysical Congress, Lisboa, 2013.

Scientific Committee Member of the 1^st Iberian Congress on Prions, Santiago de Compostela, Spain, 2012

Chairman in the 9^th International Conference on Protein Stabilisation, Lisboa, 2012.

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Chairman of the International Conference on Enzyme Technology, RELATENZ 2005, Varadero, Cuba, 2005.

Chairman of the 6^th International Conference on Protein Stabilization, Bratislava, Slovakia, 2004.

Chairman of the “Protein Folding” session in the 2^nd International Conference on Protein Stabilization, Lisboa, 2000.

Chairman of the “Biocolloids” session in the 14^th Conference of the European Colloid and Interface Society, Patras, Grécia, 2000.

Reviewer of the journals:

Archae J. Chem. Technol. Biotechnol.

Applied Microbiol. Biotechnol. J. Biotechnol.

Austin Neurol. Neurosci. J. Phys. Chem.

Biotechnol. Bioeng. J. Biol. Inorg. Chem.

Biocat. Biotrans. Langmuir

Biomacromolecules Lipids

Biotechnol. Prog. Microbial Cell Fact.

BBA - Proteins and Proteomics Nanomedicine

Biophys. J. Peer J

BMC Biochemistry PLOS One

ChemBioChem Polym. Int.

Chem. Phys. Lipids Process Biochem.

Int. Electronic J. Biotechnology Protein Sci.

Eur. J. Biochem Proteomics

FEBS J. Spectroscopy Lett.

Int. J. Biol. Macromolecules Scientific Reports Int. J. Molec. Sci.

6. GRANTS

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1. Team Member (Cryocube – Portugal 2020) “Desenvolvimento de tecnologia criogénica para substâncias biológicas” 2017-2018

2. Team Member (PTDC/CTM-NAN/2700/2012) “Nano-Dímeros Metálicos para Biosensores de Proteína Individual com Resposta Plasmónica Intensificada”

3. PI (PTDC/QUI-BIQ/119677/2010) “A biophysical approach to oxidative folding in the Endoplasmic reticulum”

4. Team Member (PTDC/MAR/122296/2010) “Bioprospecting function of an ancient gene (CRTAC) encoding a novel protein in a marine teleost by comparative analysis”

5. Team Member (PTDC/EQU-EQU/104318/2008) “Controlled freezing to improve the stability and delivery of therapeutic proteins”

6. PI (PTDC/QUI/73027/2006) “Single-Molecule Detection of the Prion Protein:

Characterization of Conformational Change Involved on Prion Diseases”

7. Team Member (PTDC/QUI/64112/2006) “Photoinduced electron transfer by artificial hemoproteins in carbon nanotubes”

8. Team Member (PTDC/QUI/64658/2006) “Single Molecule Signalling of Peptide Metal Binding”

9. PI (POCI/CVT/56668/2004) “A Murine Neuroblastoma Cell-Based Approach to Study and Prevent Prion Diseases”

10. Team Member (POCI/QUI/58816/2004) “Lipid-Lipid and Protein-Lipid Interactions in Model Systems of Cell Membranes”.

11. Team Member (POCI/QUI/57387/2004) “Molecular Organization of Functionalized Porphyrins in Giant Vesicles and Supramolecules”.

12. Team Member (Sapiens 2002) “Relation Between CYP3A4 Gene Polymorphism, Protein Structure and Activity in Disease Setting”

13. Team Member (Sapiens 2002) “Design of Stabilizing Affinity Molecular Probes: A Practical Approach to Assess and Enhance Protein Stability”

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7. PUBLICATIONS AND PATENTS

7.1.Patents

1. Patente de Invenção Nacional nº 105405, 2011 (Sistema e método de preservação, armazenamento e transporte de materiais biológicos)

7.2. Publications

1. Botequim, D., I. I. R. Silva, S. G. Serra, E.P. Melo, D. M. F. Prazeres, S. M. B.

Costa, P. M. R. Paulo (2020) Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective plasmon-enhanced emission

Nanoscale 12, 6334-6345.

2. Correia, C., E. Tavares, C. Lopes, J.G. Silva, A. Duarte, V. Geraldes, M.A.

Rodrigues, E.P. Melo^* (2020) Stability of protein formulations at sub-zero temperatures by isochoric cooling

J. Pharm. Sci. 109: 316-322.

3. Plufck, A.C.D., D.P.C. de Barros, L.P. Fonseca, E.P. Melo^* (2018) Stability of lipases in miniemulsion systems: Correlation between secondary structure and activity

Enz. Microbiol. Technol. 114: 7-14.

4. Melo, E.P., C. Lopes, P. Gollwitzer, S. Lortz, S. Lenzen, I. Mehmeti, C. F.

Kaminski, D. Ron, E. Avezov (2017) Triper, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2

BMC Biology 15: 24

5. Macedo, J.A., D. Schrama, I. Duarte, E. Tavares, J. Renault, M.E. Futschik, P.M.

Rodrigues, E.P. Melo^* (2017) Membrane-enriched proteome changes and prion protein expression during neural differentiation and in neuroblastoma cells.

BMC Genomics 18: 319

6. Anjos, L., I. Morgado, M. Guerreiro, J. C. Cardoso, E. P. Melo, D. M. Power (2017) Cartilage Acidic Protein 1 (CRTAC1), a new member of the beta-propeller protein family with amyloid propensity

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Proteins 85: 242-255.

7. Konno, K., E. P. Melo, C. Lopes, C. F. Kaminski, I. Mehmeti, S. Lenzen, D. Ron, E. Avezov (2015) ERO1-independent production of H2O2 within the endoplasmic reticulum fuels Prdx4 mediated oxidative protein folding

J. Cell Biol. 211: 253-259.

8. Estrela, N., H. G. Franquelim, C. Lopes, E. Tavares, J. A. Macedo, G. Christiansen, D. E. Otzen, E. P. Melo^* (2015) Sucrose prevents protein fibrillation through compaction of the tertiary structure but hardly affects the secondary structure

Proteins 83: 2039-2051.

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9. Avezov, E., T. Konno, A. Zyryanova, W. Chen, R. Laine, A. Crespillo-Casada, E. P.

Melo, R. Ushioda, K. Nagata, C. F. Kaminski, H. P. Harding, D. Ron (2015) Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum

BMC Biology 13: 2.

10. Tsunoda, S., E. Avezov, A. Zyryanova, T. Konno, L. Mendes-Silva, E. P. Melo, H.

P. Harding, D. Ron (2014) Intact protein folding in the glutathione-depleted endoplasmic reticulum implicates alternative protein thiol reductants

eLife 3: e03421.

11. Tavares, E., J. A. Macedo, P. M. R. Paulo, C. Tavares, C. Lopes, E. P. Melo* (2014) Live-cell FRET imaging reveals clustering of the prion protein at the cell surface induced by infectious prions

Biochim. Biophys. Acta – Molecular Basis of Disease 1842: 981-991.

12. Brissos, V., N. Gonçalves, E. P. Melo, L. O. Martins (2014) Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution

PLOS One 9: e87209.

13. Rosa, M., C. Lopes, E. P. Melo, S. K. Singh, V. Geraldes, M. A. Rodrigues (2013) Measuring and modelling hemoglobin aggregation below freezing temperature

J. Phys. Chem. B 117: 8939-8946.

14. Avezov, E., B. C. S. Cross, G. S. K. Schierle, M. Winters, H. P. Harding, E.P. Melo, C. F. Kaminski, D. Ron (2013) Lifetime imaging of a fluorescent protein sensor tracks ER thiol redox revealing its surprising stability

J. Cell Biol. 201: 337-349.

15. Anjos, L., A. S. Gomes, E.P. Melo, A. V. M. Canário, D. M. Power (2013) Cartilage acidic protein 2 a hyperthermostable, high affinity calcium-binding protein

Biochim. Biophys. Acta – Proteins and Proteomics 1834: 642-650.

16. Fernandes, A.T., C. Lopes, L. O. Martins and E.P. Melo* (2012) Unfolding pathway of CotA-laccase and the role of cooper on the prevention of refolding through aggregation of the unfolded state

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Biochem. Biophys. Res. Commun. 422: 442-446.

17. Fernandes, A.T., M. M. Pereira, C. S. Silva,P. F. Lindley, I. Bento, E. P. Melo* and L. O. Martins*(2011)The removal of a disulfide bridge of CotA-laccase changes the slower motion dynamics involved in copper binding but has no effect on the thermodynamic stability

J. Biol. Inorg. Chem. 16: 641-651.

18. Zito, E., E.P. Melo, Y.Yang, Å. Wahlander, T.A. Neubert and D. Ron (2010) Oxidative protein folding by an endoplasmic reticulum localized peroxiredoxin Molec. Cell 40: 787-797.

19. Melo, E.P.^*, N. Estrela, C. Lopes, A. C. Matias, E. Tavares and V. Ochoa-Mendes (2010) Compacting proteins: Pros and Cons of osmolyte-induced folding

Curr. Prot. Pep. Science 11: 744-751.

20. Fernandes, A.T., E.P. Melo and L.O. Martins (2010) Stability studies in the CotA- laccase from Bacillus subtilis and in the metallo-oxidase McoA from Aquifex aeolicus

Proceedings: Workshop on oxidative enzymes as sustainable industrial biocatalysts, Santiago de Compostela, Spain.

21. Madeira, C., N. Estrela, J.A.B. Ferreira, S.M. Andrade, S.M.B. Costa and E.P.

Melo* (2009) Fluorescence lifetime imaging microscopy and fluorescence resonance energy transfer (FLIM-FRET) from cyan to yellow fluorescent protein validates a novel method to cluster proteins on solid surfaces

J. Biomed. Opt. 14: 044035.

22. Fernandes, A.T., L.O. Martins and E.P. Melo* (2009) The Hyperthermophilic Nature of the Metallo-Oxidase from Aquifex aeolicus

Biochim. Biophys. Acta– Proteins and Proteomics 1794: 75-83.

23. Morgado, I., E.P. Melo, E. Lundberg, N.L. Estrela, A.E. Samer-Eriksson and D.

Power (2008) Piscine transthyretin hormone affinity and fibril formation: The role of the N-terminal

Mol. Cell. Endocrinol. 295: 48-58.

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24. Brissos, V., E.P. Melo, J.M.G. Martinho and J.M.S. Cabral (2008) Biochemical and Structural Characterisation of Cutinase Mutants in the Presence of the Anionic Surfactant AOT

Biochim. Biophys. Acta– Proteins and Proteomics 1784: 1326-1334

25. Baptista, R.P., S.H. Pedersen, G.J.M. Cabrita, D.E. Otzen, J.M.S. Cabral and E.P.

Melo* (2008) Thermodynamics and Mechanism of Cutinase Stabilization by Trehalose

Biopolymers 89: 538-547

26. Durão, P., Z. Chen, A.T. Fernandes, M.M. Pereira, E.P. Melo, and L.O. Martins (2008) Copper Incorporation Into Recombinant CotA-laccase from Bacillus subtilis:

Characterization of Cu Full-Loaded Enzymes J. Biol. Inorg. Chem. 13: 183-193

27. Melo, E.P.*, A.T. Fernandes, P. Durão and L.O. Martins (2007) Insight into Stability of Cot-A laccase from the spore coat of Bacillus subtilis

Biochem. Soc. Trans. 35: 1579-1582

28. Viseu, M.I., E.P. Melo, T.I. Carvalho, R.F. Correia and S.M.B. Costa (2007) Unfolding kinetics of β-Lactoglobulin induced by surfactant and denaturant: A stopped-flow/fluorescence study

Biophys. J. 93: 3601-3612

29. Chen, Z., C.F. Franco, R.P. Baptista, J.M.S. Cabral, A. V. Coelho, C.J. Rodrigues Jr and E.P. Melo* (2007) Purification and identification of cutinases from Colletotrichum kahawae and Colletotrichum gloeosporioides

Appl. Microbiol. Biotechnol. 73: 1306-1313

30. Neves-Petersen, M.T., S. Klitgaard, A.S.L. Carvalho, S.B. Petersen, M.R. Aires- Barros, E.P. Melo (2007) Photophysics and photochemistry of horseradish peroxidase A2 upon UV illumination

Biophys. J. 92: 2016-2027.

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31. Otzen, D.E., L. Giehm, R.P. Baptista, S.R. Kristensen, E.P. Melo, S. Pedersen (2007) Aggregation as the basis for complex behaviour of cutinase in different denaturants

Biochim. Biophys. Acta– Proteins and Proteomics 1774: 323-333.

32. Carvalho A.S.L., B.S. Ferreira, M.T. Neves-Petersen, S.B. Petersen, M.R. Aires- Barros and E.P. Melo* (2007) Thermal denaturation of HRPA2: pH-dependent conformational changes.

Enz. Microbiol. Technol. 40: 696-703.

33. Pedersen, S., L. Nesgaard, R.P. Baptista, E.P. Melo, S.R. Kristensen and D.E. Otzen (2006) pH-dependent aggregation of cutinase is efficiently suppressed by 1,8-ANS Biopolymers 83: 619-629

34. Durão, P., I. Bento, André T. Fernandes, E.P. Melo, P. F. Lindley and L. O. Martins (2006) Perturbation of the T1 copper site in CotA-laccase from Bacillus subtilis:

Structural, Biochemical, Enzymatic and Stability Studies J. Biol. Inorg. Chem. 11: 514-526.

35. Chen, L.Y., J.A.B. Ferreira, S.M.B. Costa, G.J.M. Cabrita, D.E. Otzen and E.P.

Melo* (2006) Compaction of ribosomal protein S6 by sucrose occurs only under native conditions

Biochemistry 45:2189-2199.

36. Rodrigues, M.L., P. Martel, M. Archer, S. Miranda, M. Thomaz, F.J. Enguita, R.P.

Baptista, E.P. Melo, N. Sousa, A. Cravador and M.A. Carrondo (2006) Crystal structures of the free and sterol-bound forms of β-cinnamomin

Biochim. Biophys. Acta 1746: 110-121.

37. Chen, L.Y., G.J.M. Cabrita, D.E. Otzen and E.P. Melo* (2005) Stabilization of the ribosomal protein S6 by trehalose is counterbalanced by the formation of a putative off-pathway species

J. Mol. Biol. 351: 402-416.

38. Carvalho A.S.L., M.T. Neves-Petersen, S.B. Petersen, M.R. Aires-Barros and E.P.

Melo* (2005) Formation of a misfolded conformation during refolding of HRPA1 in the presence of calcium

Biochim. Biophys. Acta 1747: 99-107.

39. Carvalho A.S.L., A.M. Santos, M.T. Neves-Petersen, S.B. Petersen, M.R. Aires- Barros and E.P. Melo* (2004) Conformational states of HRPA1 induced by thermal unfolding: Effect of low molecular weight solutes

Biopolymers 75: 173-186.

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40. Carvalho, A.S.L., E.P. Melo*, M.T. Neves-Petersen, S.B. Petersen and M.R. Aires- Barros (2003) Heme and pH-Dependent Stability of an Anionic Horseradish Peroxidase.

Arch. Biochem. Biophys. 415: 257-267.

41. Melo E.P.*, L.Y. Chen, J.M.S. Cabral, P. Fojan, S.B. Petersen and D.E. Otzen (2003) Trehalose favors a cutinase compact intermediate off-folding pathway.

Biochemistry 42: 7611-7617.

42. Melo E.P.*, S.M.B. Costa, J.M.S. Cabral, P. Fojan and S.B. Petersen (2003) Cutinase-AOT interactions in reverse micelles: The effect of 1-hexanol.

Chem. Phys. Lipids 124: 37-47.

43. Melo E.P.*, R.P. Baptista and J.M.S. Cabral (2003) Improving Cutinase Stability in Aqueous Solution and in Reverse Micelles by Media Engineering.

J. Molec. Catalysis B: Enzymatic 22: 299-306.

44. Baptista R.P., L.Y. Chen, A. Paixão, J.M.S. Cabral and E.P. Melo* (2003) A novel pathway to enzyme deactivation: The cutinase model.

Biotechnol. Bioeng. 82: 851-857.

45. Melo E.P.*, T.Q. Faria, L.O. Martins, A.M. Gonçalves and J.M.S. Cabral (2001) Cutinase unfolding and stabilization by trehalose and mannosylglycerate.

Proteins 42: 542-552.

46. Baptista R.P., J.M.S. Cabral and E.P. Melo* (2000) Trehalose delays the reversible but not the irreversible thermal denaturation of cutinase.

Biotechnol. Bioeng. 70: 699-703.

47. Melo E.P.*, M.A. Taipa, M.R. Castellar, S.M.B. Costa and J.M.S. Cabral (2000) A spectroscopic analysis of thermal stability of the Chromobacterium viscosum lipase.

Biophys. Chem. 87: 111-120.

48. Melo E.P.*, P. Fojan, J.M.S. Cabral and S.B. Petersen (2000) Dynamic light scattering of cutinase in AOT reversed micelles.

Chem. Phys. Lipids 106: 181-189.

49. Carvalho, C.M.L., E.P. Melo, S.M.B. Costa, J.M.S. Cabral and M.R. Aires-Barros (1999) A steady-state fluorescence study of cutinase microencapsulated in AOT reversed micelles at optimal stability conditions.

Biotechnol. Lett. 21: 673-681.

50. Melo, E.P.*, C.M.L. Carvalho, M.R. Aires-Barros, S. M. B. Costa and J. M.S.

Cabral (1998) Deactivation and conformational changes of cutinase in reverse micelles. Biotechnol. Bioeng. 58, 380-386.

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51. Melo, E.P.*, M.R. Aires-Barros, S. M. B. Costa and J. M.S. Cabral (1997) Thermal Unfolding of Proteins at High pH Range Studied by UV absorbance.

J. Biochem. Biophys. Methods 34, 45-59.

52. Carneiro-da-Cunha, M.G., E.P. Melo, J. M.S. Cabral and M.R. Aires-Barros (1996) Liquid-liquid extraction of a recombinant cutinase from fermentation media with AOT reversed micelles.

Bioprocess Eng. 15: 151-157.

53. Melo, E.P., S. M. B. Costa and J. M.S. Cabral (1996) Denaturation of a recombinant cutinase from Fusarium solani in AOT-iso-octane reverse micelles. A steady-state fluorescence study.

Photochem. Photobiol. 63: 169-175.

54. Setti, L., P. Fevereiro, E.P. Melo, P.G. Pifferi, J.M.S. Cabral and M.R. Aires-Barros (1995) Superactivity of peroxidase solubilized in reverse micellar systems.

Appl. Biochem. Biotechnol. 55: 207-218

55. Melo, E.P., M.G. Ivanova, M.R. Aires-Barros, J.M.S. Cabral and R. Verger (1995) Glyceride synthesis catalyzed by cutinase using the monomolecular film technique.

Biochemistry 34: 1615-1621

56. Melo, E.P., M.R. Aires-Barros and J.M.S. Cabral (1995) Tryglicerides hydrolysis and stability of a recombinant cutinase from Fusarium solani in AOT-iso-octane reversed micelles.

Appl. Biochem. Biotechnol. 50:45-56

57. Melo, E.P., S.M.B. Costa and J.M.S. Cabral (1995) Structural effect of reversed micelles of AOT over a recombinant cutinase from Fusarium solani pisi. A steady state fluorescence study.

Ann. N.Y. Acad. Sci. 750:85-88

58. Melo, E.P. and N.T. Rodeia (1992) Nitrogen sources influencing the synthesis of - glucosidase from Fistulina hepatica (Huds.) Fr.

Bol. Soc. Port. Ciências Naturais 25:47-54

59. Barreto, M.T.O., E.P. Melo, J.S. Almeida, A.M.R.B. Xavier and M.J.T. Carrondo (1991) A kinetic method for calculating the viability of lactic starter cultures.

Appl. Microbiol. Biotechnol. 34:648-652

60. Barreto, M.T.O., E.P. Melo, J.L. Moreira and M.J.T. Carrondo (1991) High Cell Density Reactors for the Production of Lactobacillus plantarum.

J. Industrial Microbiol. 7:63-70

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61. Almeida, J.S., M.T.O. Barreto, J.P.S.G. Crespo, L.M.D. Gonçalves, E.P. Melo, A.M.R.B. Xavier and M.J.T. Carrondo (1990) UF membrane Cell Recycle System for Continuous Culture of Acidogenic Bacteria.

Proceedings: 5_thWorld Filtration Congress, Nice.

62. Barreto, M.T.O., E. P. Melo and M.J.T. Carrondo (1989) Starter Culture production in Fluidized Bed Reactor with a Flocculent Strain of L. plantarum.

Biotechnol. Lett. 11:337-342

7.2. Book Chapters

1. Martins, L. O., E. P. Melo, A. Sanchez-Amat and M. P. Robalo

Bacterial Laccases: Some recent advances and applications. In: Laccases (and other multi-copper oxidases) in bioremediation of pollutants and xenobiotiocs.

Microbiology Monographs series, 2020, Springer, Heidelberg 2. Melo, E.P.

Estabilidade de Proteínas. In: Engenharia Enzimática (M. Gama, M.R. Aires-Barros and J.M.S. Cabral, eds), LIDEL, Portugal, 2003, 69-100.

3. Melo, E.P.*, M.R. Aires-Barros and J.M.S. Cabral

Reverse Micelles and Protein Biotechnology. Biotechnol. Ann. Rev., 2001, 7: 87-129.

4. Ransac, S., F. Carrière, E. Rogalska, R. Verger, F. Marguet, G. Buono, E.P. Melo, J.M.S. Cabral, M.P. Egloff, H. van Tilbeurgh, and C. Cambillau

The kinetics, Specificities and Structural Features of Lipases. In: Molecular Dynamics of Biomembranes (J. den Kamp, ed.), Springer-Verlag, Berlin, Heidelbergh, 1996, 265-304.

5. Ransac, S., F. Carrière, E. Rogalska, R. Verger, F. Marguet, G. Buono, E.P. Melo, J.M.S. Cabral, M.P. Egloff, H. van Tilbeurgh, and C. Cambillau

The kinetics, Specificities and Structural Features of Lipases. In: Engineering of/with Lipases (F.X. Malcata, ed.), Kluwer Academic Publishers, Dordrecht, Holanda, 1996, 143-182.

6. Carneiro-da-Cunha, M.G., E.P. Melo, M.J. Sebastião, M.R. Aires-Barros and J.M.S.

Cabral

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Separation and purification of lipases by liquid-liquid extraction processes. In:

Engineering of/with Lipases (F.X. Malcata, ed.), Kluwer Academic Publishers, Dordrecht, Holanda, 1996, 551-576.

6. Melo, E.P., A.P. Gonçalves, M.J. Sebastião, P.J. Cunnah, C.M.L. Carvalho, J.M.

Madeira Lopes, F. Lemos, M.R. Aires-Barros and J.M.S. Cabral

Kinetics and stability of cutinase immobilized in reverse micelles and zeolites. In:

Engineering of/with Lipases (F.X. Malcata, ed.), Kluwer Academic Publishers, Dordrecht, Holanda, 1996, 297-327.