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A novel 3D fibril force assay implicates src in tumor cell force generation in collagen networks.

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Academic year: 2017

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Figure 2. Localization of GFP-ca-Src at the plasma membrane and in focal adhesions and invadopodia core complexes associated with matrix degradation
Figure 3. 3D views of example fixed cells. Examples of each cell line fixed for the Src localization analysis (Fig
Figure 7. Assay results. MDA-MB-231 cells transfected with GFP-tagged constitutively active Src exert nearly twice as much total force on the gels via the collagen network as MDA-MB-231s transfected with GFP-tagged wild-type Src (A, p,0.005), while both ce
Figure 8. Cell Shape, Src location, and total Src. (A) MDA-MB-231 cells transfected with GFP-tagged constitutively active Src exert tended to have a higher surface area to volume ratio (p , .001) as well as tending to be larger in volume and have larger su

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