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Alteration of cellular behavior and response to PI3K pathway inhibition by culture in 3D collagen gels.

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Academic year: 2017

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Figure 1. Simplified PI3K/AKT pathway map. In this study we examine the levels of PI3K, mTOR and phosphorylated Protein S6 as indicators of cellular response to PI3K/mTOR inhibition.
Figure 3. Proliferation of U2OS in 3D collagen gels with different concentration of live cell dye CMRA
Figure 6. Migration of U2OS cells on standard 2D tissue culture plastic compared to 3D collagen gels
Figure 7. Protein expression blots from U20S cells grown on tissue culture plastic or in 3D collagen gels
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