A trifunctional dextran-based nanovaccine targets and activates murine dendritic cells, and induces potent cellular and humoral immune responses in vivo.

The BM microenvironment consists of various extracellular matrix proteins, and cell components as haematopoietic stem cells, progenitor and precursor cells, immune cells,

Mean IFN- ␥ ELISPOT response by spleen and LN cells in vaccinated and control C57BL/6 and IL-12p40 − / − mice after infection with L.. After undisturbed incubation for 20 h, cells

Graft receptor BALB/c mice received spleen cells (30, 90, 150 or 375 x 10 6 ) from donor C57BL/6 mice intragastrically on three successive days, starting seven days before the

(B) THP-1 cells were cultured (2 6 10 6 cells/ml) with DH-PS (100 m g/ml) or PBS and supernatants were collected in indicated time points for IL-1ra measurements..

neoformans cells were incubated with the appropriate photosensitizer (10 m M methylene blue, rose bengal, EtNBSe,.. and pL-ce6 for 30 min) as previously described, and the cells

Peripheral blood mononuclear cells (PBMC) plated at 1 6 10 6 cells/ml were isolated and cultured as previously described [50]. Purified CD3 + T-cells and monocytes with and without

1: innate and adaptive immune response of intestinal murine dendritic cells (DCs) infected with enteroinvasive Escherichia coli (EIEC) or Shigella flexneri.. Small

Two weeks after the last immunization with HIVBr18 or the empty vector pVAX1, spleen cells from 6 BALB/c mice were labeled with CFSE (1.25 m M) and cultured for 4 days in the