ISSN: 1804-1884 (Print) 1805-5014 (Online) Volume 5, 2011, pp. 115-125
The paper discusses results of screening research made in vegetable extracts of Uzbekistan’s flora in order to expose new, effective antiatherosclerotic drugs. The hypolipidemic feature in 106 vegetable extracts were researched in vivo mice with endogenous and ethanol hyperlipidemia with the definition of the content of overall cholesterol, triglycerides, general lipids and cholesterol lipoproteins in high concentration.
1
A
DOLATZ
IYAEVA,
2
N
OSIRY
ULDASHEV1
Republican Specialized Centre of
Cardiology,
2Tashkent Pediatric
Medical Institute, Uzbekistan
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Flora
1represents a rich opportunity for developing highly active preparations, including
those for treatment of cardiovascular diseases. In territory of the former Soviet Union 20
thousand species of medical plants grow. About 30% of known plants in the CIS grows in
Uzbekistan. Many of them are widely used in cardiology practice for the prevention and
treatment of atherosclerosis, myocardial infarction, as antiarrhythmic agents.
High morbidity and mortality from atherosclerosis makes one of the most significant the
policy of the disease effective prevention and pharmacotherapy (Akopov, 1990).
Meanwhile modern antiatherosclerotic (mainly lipid/lowering) drugs are likely to provoke
a number of side effects and complications, even stimulation of carcinogenesis (Аripov
and Fesenko, 2000). Taking into account the necessity of durational appliance of this type
of drugs it was considered reasonable to conduct the search of hyperlipidemic agents
among the plants, since they are close to the organism of mammals in terms of evolution
and that is why they are well tolerated by the patients on durational appliance
(Devidenkova and Shafran, 1998).
The experiments were performed on 659 white mongrel male rats. During the series of
the experiments which lasted five days, Plant extracts were administered per os at a dose
of 10 mg/kg. On the 6th day hyperlipidemia (endogenous) was provoked by fasting
animals for 24hours without limiting consumption of water (Mesherskaya and Sonina,
1972). Ethanol hyperlipidemia was induced by injecting 50% ethanol per os in doses: on
the first day of the experiment 2g/kg, on the second day / 4g/kg, on the third day / 6g/kg
body weight (Leontiva and Rijenkov, 1979).
Animals which were under etheric narcosis were slaughtered by decapitation. The research
was conducted on the blood serum and erythrocytic mass. The concentrations of overall
cholesterol (OChS), triglycerides (TG), cholesterol high/density lipoprotein (ChS HDL)
were assessed by enzymatic method on auto analyzer “Daytona” (Randon, UK); the
content of overall lipid (OL) / via the colorimetric method using panel “Lechama” (Czech
Republic); the concentration of ceruloplasmin (CP) was found by the Ravin method, the
rate of peroxide haemolysis erythrocyte (PHE) was defined as well.
Statistical processing of numerical data was conducted by standard methods of variation
statistics using Student’s t/test for assessing reliability of differences in Excel/2000
program. Mean values were represented as M ± m (mean ± average error of the mean).
The research revealed the range of considerable changes in lipidic spectrum of blood of
animals on experimental endogenous hyperlipids (Table 1). The obtained data prove that
under the endogenous hyperlipidemia the level of OChS and TG in blood serum makes
up to 1.84 ± 0.15 mmol/l and 1.03 ± 0.05 mmol/l, respectively, that on 71.9 and 43,1%
higher in comparison with such indices of intact animals (1.07 ± 0.03 mmol/l and 0.73 ±
0.041 mmol/l).
T
ABLE1.
S
CREENING OF VEGETABLE EXTRACTS ON PRESENCE OF ANTIOXIDANT AND MEMBRANE STABILIZER EFFECT UNDER ENDOGENOUS HYPERLIPIDEMIAName of Extracts ОСhS, mmol/l TG, mmol/l СhS HDL, mmol/l OL, g/l
1 2 3 4 5
Intact group 1.07+0.03 0.72+0.041 0.6+0.077 4.35+0.17
Controlled group 1.84+0.15 1.03+0.05 0.47+0.033 6.61+0.26
Plantago lanceolata (R) 1.69±0.15* 1.03±0.12* 0.39±0.04* 7.35±0.18*.**
Rosa canina (Sk) 1.59±0.25* 1.08±0.05* 0.35±0.08* 7.28±0.49*
Cratalgus songarica (R) 1.56±0.13* 1.11±0.19* 0.35±0.06* 8.07±0.17*.**
Cynodon dactulon (R) 1.90±0.61* 1.07±0.12* 0.33±0.11* 5.82±0.31
Prunus sogdiana (R) 1.55±0.26* 0.97±0.12* 0.39±0.03* 8.34±0.96*.**
Rosa canina (R) 1.65±0.14* 0.94±0.1* 0.37±0.11* 5.3±0.22
Crataegus pontica (L) 1.55±0.2* 1.06±0.06* 0.4±0.08* 7.68±0.65*.** Iuniperus zeravshanica (Fr) 1.32±0.25 0.96±0.03* 0.39±0.09* 7.3±0.72*
Cynodon dactulon (O) 1.43±0.32 1.07±0.05* 0.35±0.05* 5.9±0.6
Rosa canina (Fr) 1.63±0.2* 0.92±0.09* 0.45±0.01* 5.89±0.39
Picnomon acarna (O) 1.91±0.11* 0.96±0.13* 0.43±0.05* 6.97±0.91*
Alhagi pseudalhagi (R) 1.84±0.16* 1.01±0.09* 0.48±0.08 7.46±0.75*.**
Rosa canina (L) 1.78±0.06* 0.95±0.13* 0.44±0.04 7.79±0.96*.**
Prunus sogdiana (Fr) 1.83±0.16* 0.95±0.15* 0.44±0.08 7.43±0.9*.**
Chenopodium album (Fr) 1.96±0.23* 1.07±0.02* 0.42±0.06 5.37±0.85
Rubus caesius (L) 1.77±0.13* 0.89±0.09 0.45±0.09 7.3±1.4*
Chenopodium album (R) 1.99±0.2* 0.94±0.05* 0.38±0.02* 6.3±0.39*
Amaranthus retroplexus (L) 1.9±0.04* 1.01±0.04* 0.35±0.06* 6.74±0.97*
Prunus sogdiana (L) 1.72±0.15* 0.89±0.1 0.47±0.09 7.23±2.08*
Amaranthus retroplexus (St) 1.78±0.15* 0.89±0.07 0.41±0.07 5.23±1.15 Amaranthus retroplexus (Fr) 1.74±0.12* 0.9±0.06 0.49±0.17 6.94±1.17*
Prunus sogdiana (Sk) 1.85±0.11* 0.98±0.05* 0.47±0.08 5.42±1.35
Prunus sogdiana (R) 1.95±0.11* 1.01±0.08* 0.48±0.07 5.9±1.59*
Prunus sogdiana (O) 1.68±0.14* 0.97±0.14* 0.47±0.07 7.32±0.59*
Pheum Fedtshenokovi (F) 1.86±0.19* 0.98±0.11* 0.43±0.07 5.54±1.41 Atraphaxis pyrifolia (Sk) 1.89±0.1* 1.06±0.09* 0.45±0.02 5.26±0.5 Atraphaxis pyrifolia (St) 1.96±0.28* 1.04±0.04* 0.51±0.1 6.08±0.55* Atraphaxis pyrifolia (R) 2.14±0.08* 0.89±0.08 0.39±0.11* 7.79±1.03* Amygdalis spinosissima (S) 1.75±0.05* 0.95±0.18* 0.43±0.06 6.32±1.1* Cerasus erythrocapra (L) 1.86±0.12* 0.96±0.06* 0.35±0.03* 7.89±0.69*
Avena sativa (S) 0.97±0.06** 0.66±0.08** 0.54±0.05** 4.21±0.13**
Amygdalis bucharica (L) 1.97±0.23* 1.04±0.19* 0.35±0.04* 6.89±0.96*
Trifolium pratense (F) 1.88±0.04* 0.94±0.11* 0.43±0.03 5.87±1.35
Amygdalis bucharica (Sk) 1.87±0.2* 0.86±0.08 0.43±0.11 4.22±0.57** Pheum Fedtshenokovi (L) 1.72±0.1* 0.93±0.06* 0.42±0.02 4.87±0.89**
Amygdalis bucharica (L) 1.65±0.18* 0.9±0.17 0.45±0.04 7.47±0.86*
Solidago dahurica Kitag (O) 1.72±0.1* 0.96±0.08* 0.45±0.08 7.4±0.49* Acroptilon repens ( L)Op.(O) 1.73±0.06* 0.95±0.07* 0.36±0.03* 6.458±0.8* Melandrium turkestanicus (Rgl)Vved (R) 1.72±0.14* 0.94±0.07* 0.46±0.04 7.55±0.79*
Inula helenium (R) 1.81±0.1* 1.03±0.11* 0.45±0.02 7.42±1.26*
Artemisia vulgaris h. (O) 1.87±0.03* 0.98±0.04* 0.37±0.06* 7.75±1.67* Berberis integerrima (Bge) (Fr) 1.71±0.12* 0.93±0.1* 0.35±0.06* 7.89±0.94* Juniperus turkestanica Kom (L) 1.01±0.1** 0.72±0.05** 0.56±0.03 4.59±0.22**
Solonum nigra h. (Fr) 2.0±0.21* 0.98±0.11* 0.42±0.06 5.28±1.39
Solidago dahurica kitag ® 1.77±0.14* 0.98±0.12* 0.4±0.02* 6.51±1.31* Melandrium turkestanicus (Rgl)Vved (O) 1.9±0.06* 0.98±0.08* 0.42±0.06 5.09±1.73
Acroptilon repens (F) 1.85±0.14* 0.83±0.06 0.35±0.07* 5.1±0.95
Trifolim pratense h.(F) 1.67±0.05* 1.01±0.09* 0.42±0.06 7.03±1.2* Cousinia saawertziwii Regel (O) 1.92±0.11* 1.05±0.21* 0.44±0.06 7.09±0.49* Anaphalis Velutina Krasch (O) 1.87±0.13* 0.94±0.06* 0.43±0.02 4.96±0.98**
Astragalus sp. (O) 1.75±0.08* 1.12±0.05* 0.44±0.06 7.68±0.34*
Berberis oblonga (Rgl) Schneid ® 1.84±0.05* 0.96±0.03* 0.39±0.01* 6.29±0.48* Hieracium procerum Fr ® 1.83±0.16* 0.92±0.11* 0.46±0.07 6.98±1.31* Stachys betoniciflora rupr. ® 1.84±0.11* 0.96±0.2* 0.46±0.04 4.96±1.49** Acantholimon erythraeum Bge (A) 1.0±0.07** 0.79±0.06** 0.55±0.05** 4.64±0.48** Pulicaria salviifolia Bunge (R) 1.76±0.06* 1.02±0.1* 0.45±0.09 7.85±2.16* Psoralea drupacea Bunge (S) 1.78±0.18* 1.01±0.2* 0.47±0.03 7.91±1.43* Trifolim pratense h.(O) 1.74±0.06* 0.99±0.09* 0.41±0.05 6.48±0.48* Melandrium turkestanicus (Rgl)Vved (Fr) 1.91±0.13* 1.1±0.17* 0.38±0.07* 5.48±0.57 Artemisia vulgaris h. (R) 1.91±0.1* 1.13±0.25* 0.43±0.05 6.94±0.37* Descurania Sophia (L) Schur (Fr) 0.98±0.08** 0.73±0.06** 0.53±0.04** 4.39±0.39** Xantium strumarium h. (St) 1.78±0.17* 0.85±0.07 0.45±0.04 7.8±0.27* Conum maculatum h. (St) 1.78±0.16* 0.91±0.05* 0.44±0.05 6.56±0.49* Cortusa turkestanica A.Los (O) 1.81±0.09* 0.89±0.06 0.38±0.04* 6.2±1.0* Tanasetopsis mucronatun S kovalev (O) 1.85±0.18* 0.9±0.06* 0.5±0.1 6.43±0.1* Xantium strumarium h. (S) 1.79±0.08* 0.81±0.1 0.37±0.09* 7.8±0.3* Herniaria glabra h. (O) 1.76±0.13* 0.84±0.05 0.37±0.04* 7.98±1.04* Leonurus turkestanicusV.Krecr et Kupr (O) 1.88±0.09* 0.91±0.1* 0.44±0.12 6.57±0.91* Arctium tomentisum Mill (R) 1.76±0.18* 1.1±0.04* 0.46±0.07 4.57±0.87** Ziziphora pedicillate Parii et Vved (R) 1.74± 0.12* 0.93±0.11* 0.39±0.07* 8.38±1.2* Solidago dahurica Kitag (F) 1.7±0.14* 0.93±0.1* 0.47±0.03 7.2±0.6* Hierasiun procerum Fr (O) 1.56±0.06* 0.91±0.06* 0.37±0.18* 6.17±0.51*
Solonum nigra h. (St) 1.91±0.08* 0.98±0.13* 0.44±0.03 7.45±0.82*
Agrimonia asiatica Ius (R) 1.83±0.05* 0.92±0.12* 0.33±0.07* 6.41±1.24* Xantium strumarium h.(R) 1.82±0.06* 0.91±0.11* 0.34±0.07* 5.29±1.3 Cortusa turkestanica A. Los (F) 1.99±0.08* 1.04±0.14* 0.52±0.06 5.88±1.44 Artemisia vilgaris L.(F) 1.67±0.04* 0.93±0.06* 0.42±0.06 6.5±0.21* Chamenerium angustibolium (L) Scop (R) 1.82±0.13* 1.04±0.12* 0.4±0.17* 6.91±0.51* Stachyopsys oblongata M. Popet Vved (O) 1.84±0.05* 0.9±0.09* 0.51±0.1 8.02±0.79* Stachys betoniciflora Rupr (F) 1.9±0.18* 1.0 ±0.1* 0.54±0.15** 6.11±0.82* Ziziphora pedicillate Parii et Vved (St) 1.73±0.1* 0.96±0.11* 0.49±0.08 7.56±0.82* Spiraea pilosa Franch (O) 1.93±0.05* 0.94±0.11* 0.38±0.05* 7.83±1.02*
Acroptilon repens (R) 1.65±0.12* 0.84±0.04 0.36±0.07* 7.39±1.3*
Solonum nigra h. (R) 1.78±0.16* 1.03±0.1* 0.52±0.08 6.01±1.1*
Phragmites communis Trin (St) 1.89±0.07* 1.14±0.09* 0.51±0.1 6.78±0.23* Cortusa turkestanica A. hos (R) 1.8±0.03* 0.86±0.08* 0.4±0.02* 4.29±0.97** Trigonella pamirica Boriss (A) 1.0±0.12** 0.76±0.74** 0.54±0.08 4.54±0.57**
Astragalus sp. (R) 1.86±0.17* 0.98±0.13* 0.34±0.08* 7.53±0.76*
Trifolium pratense L. (R) 1.73±0.13* 0.98±0.24* 0.42±0.11 7.98±0.62*
Solonum nigra h. (L) 1.79±0.2* 0.96±0.15* 0.41±0.05 6.7±0.41*
Serophularia umbrosa Dumort (O) 1.69±0.13* 0.85 ±0.18 0.54±0.15** 8.04±0.7* Siderities montana L (St) 1.89±0.12* 0.94±0.1* 0.43±0.06 6.01±0.73* Phlomis olgae Rgl (R) 1.01±0.1** 0.74±0.07** 0.54±0.06** 4.5±0.22** Serratula alatavica c.A.M. (F) 1.83±0.11* 1.03±0.16* 0.38±0.07* 4.84±0.52** Serratula alatavica c.A.M.(O) 1.89±0.08* 0.97±0.08* 0.37±0.07* 7.15±0.68*
Inula helenium L (F) 1.66±0.04* 0.95±0.13* 0.43±0.04 7.6±1.18*
Psoralea drupacea Bunge (R) 1.77±0.2* 0.96±0.17* 0.45±0.11 8.99±0.4*
Inula helenium L (L) 1.8±0.19* 1.06±0.08* 0.43±0.1 7.05±1.09*
Phlomis sewerzowii Rgl (O) 1.38±0.17* 0.96±0.16* 0.47±0.01 6.24±0.5* Note: F - flower; R- root; S - seed; FR - fruit; L - leaves; St - stem; O - overground part; Sk - skin; E - entire plant; I - inflorescence; A - upper part of overground part. Authentic difference Р<0,05: * - from intact group; ** - from controlled group
On the contrary, the content of ChS HDL decreased to 21.7% and composed 0.47 ±
0.033 mmol/l opposite to 0.6 ± 0.036 mmol/l in norm. Endogenous hyperlipidemia
resulted in increasing the rate of OL in blood serum by 52.0% which made up to 6.61 ±
0.26 g/l, in intact animal group analogous accounted for 4.35 ± 0.17 g/l.
The great number of works focusing on lipids peroxidation (LPO) processes in aetiology
and pathogenesis of atherosclerosis has emerged recently. It is well known that control of
LPO in organism processes is accomplished by antioxidative systems (АОS) (Vidal, 2002;
Tertov, 1999). CP is considered to be the most important antioxidant in blood serum.
This made reasonable to study the effect which the extract delivers on the level of serum
in the blood. The findings showed that under experimental endogenous hyperlipidemia
the content of CP in blood serum drops 1.2 times in comparison with the indices of intact
animal group (Table 2). Thus, level of blood serum of intact animals and controlled group
accounted for 16.82 ± 2.24 ml/dl and 13.08 ± 1.38 ml/dl, respectively. Decrease in the
level of antioxidants including CP can lead to increase of auto/oxidation of lipidic
membrane erythrocyte, which reduces the resistance to haemolysis. In connection with
this, we studied PHE in erythrocytic mass. Under endogenous hyperlipidemia the
tendency to increase for PHE was observed: in the intact group given index made up
13.87 ± 0.66 %, and in controlled / 16.18 ± 0.12%.
T
ABLE2.
T
HE SCREENING OF VEGETABLE EXTRACTS ON PRESENCE OF HYPOLIPIDEMIC EFFECT UNDER ENDOGENOUS HYPERLIPIDEMIAName of Extracts CP, mg/100 ml PHE, %
1 2 3
Intact group 16.82+2.24 13.87+0.66
Controlled group 13.08+1.38 16.18+0.12
Plantago lanceolata (R) 11.75±2.19* 15.4±0.11
Rosa canina (Sk) 13.69±3.3 14.0±0.08
Cratalgus songarica (R) 10.96±2.84* 16.0±0.1
Cynodon dactulon (R) 12.82±2.35* 12.13±0.1
Prunus sogdiana (R) 8.4±1.74*.** 13.2±0.13
Rosa canina (R) 8.91±1.39* 14.9±0.1
Crataegus pontica (L) 10.17±2.69* 15.2±0.13
Iuniperus zeravshanica (Fr) 12.37±3.48* 17.1±0.1
Cynodon dactulon (O) 17.37±3.7** 18.1±0.09*
Rosa canina (Fr) 15.99±2.7** 17. 0±0.11
Picnomon acarna (O) 15.89±2.38** 13.1±0.21
Alhagi pseudalhagi (R) 8.25±1.98*.** 13.2±1.05
Rosa canina (L) 10.59±1.59* 14.7±1.0
Prunus sogdiana (Fr) 16.08±3.08** 17.0±0.15
Chenopodium album (Fr) 14.19±2.26 18.3±0.71*
Rubus caesius (L) 6.73±2.34*.** 19.2±0.12*
Chenopodium album (R) 13.15±2.01* 14.3±0.45
Amaranthus retroplexus (L) 9.46±1.99* 13.3±0.6
Amaranthus retroplexus (St) 13.72±3.48* 17.0±0.71
Amaranthus retroplexus (Fr) 6.72±1.81*.** 14.3±0.19
Prunus sogdiana (Sk) 12.8±2.95* 15.3±0.21
Prunus sogdiana (R) 13.3±1.54 18.0±1.3*
Prunus sogdiana (O) 9.45±2.18* 17. 0±0.43
Pheum Fedtshenokovi (F) 6.89±2.92*.** 13.2±0.47
Atraphaxis pyrifolia (Sk) 6.36±1.31*.** 15.0±0.53
Atraphaxis pyrifolia (St) 6.56±1.15*.** 13.7±0.79
Atraphaxis pyrifolia (R) 8.73±4.59* 17.2±0.42
Amygdalis spinosissima (S) 7.89±1.6* 12.3±0.24
Cerasus erythrocapra (L) 10.62±1.48* 14.2±0.73
Avena sativa (S) 17.46±1.04** 17.0±0.15
Amygdalis bucharica (L) 6.31±2.17*.** 13.7±0.79
Trifolium pratense (F) 10.62±1.84* 19.2±0.71*
Amygdalis bucharica (Sk) 14.53±2.89 14.2±0.48
Pheum Fedtshenokovi (L) 11.72±1.84* 17.9±1.24
Amygdalis bucharica (L) 11.52±3.26* 15.0±0.15
Trifolium repens (O) 7.71±1.49* 18.4±0.13
Solidago dahurica Kitag (O) 12.44±1.69* 12.3±0.72
Acroptilon repens ( L)Op.(O) 6.48±1.71*.** 17.2±0.49 Melandrium turkestanicus (Rgl)Vved (R) 14.31±4.31 12.3±0.73
Inula helenium (R) 13.9±2.7 15.0±0.28
Artemisia vulgaris h. (O) 8.24±1.18* 16.3±0.39
Berberis integerrima (Bge) (Fr) 14.1±3.13 14.4±0.47
Juniperus turkestanica Kom (L) 15.03±2.59** 17.2±0.78
Solonum nigra h. (Fr) 16.88±1.31** 15.0±0.43
Solidago dahurica kitag ® 15.02±3.88** 12.3±0.47
Melandrium turkestanicus (Rgl)Vved (O) 7.5±1.27* 17.4±0.78
Acroptilon repens (F) 8.0±2.77* 13.9±0.49
Trifolim pratense h.(F) 13.51±1.34 14.2±0.17
Cousinia saawertziwii Regel (O) 6.07±1.93*.** 15.3±0.79
Anaphalis Velutina Krasch (O) 14.71±2.16 17.3±0.48
Astragalus sp. (O) 9.67±2.02* 14.3 ±0.48
Berberis oblonga (Rgl) Schneid ® 10.35±1.85* 14.7±0.79
Hieracium procerum Fr ® 12.49±3.59* 12.3±0.47
Stachys betoniciflora rupr. ® 9.58±0.6* 17. 3±0.13
Acantholimon erythraeum Bge (A) 14.55±1.96 18.4±0.49* Pulicaria salviifolia Bunge (R) 14.62±1.69 18.2±0.74*
Psoralea drupacea Bunge (S) 15.57±1.49 20.5±0.95*
Trifolim pratense h.(O) 8.32±2.85* 14.2±0.24
Melandrium turkestanicus (Rgl)Vved (Fr) 8.84±2.65* 12.3±0.71
Artemisia vulgaris h. (R) 13.47±1.84 11.7±0.83
Descurania Sophia (L) Schur (Fr) 15.79±1.95 17.3±0.49
Xantium strumarium h. (St) 17.37±2.51** 12.9±0.55
Conum maculatum h. (St) 13.47±3.42 11.8±0.67
Cortusa turkestanica A.Los (O) 9.68±3.0* 16.2±0.79
Tanasetopsis mucronatun S kovalev (O) 14.9±2.61 17.8±0.97
Xantium strumarium h. (S) 18.1±5.48** 13.2±0.42
Herniaria glabra h. (O) 13.4±3.55 10.0±0.85
Leonurus turkestanicusV.Krecr et Kupr (O) 11.85±1.52* 11.2±1.35
Arctium tomentisum Mill (R) 11.62±2.92* 12.1±0.67
Ziziphora pedicillate Parii et Vved (R) 7.8±0.69* 19.7±1.43
Solidago dahurica Kitag (F) 12.84±1.61* 14.7±0.81
Hierasiun procerum Fr (O) 10.5±3.06* 15.6±0.67
Solonum nigra h. (St) 9.48±11.13* 12.6±0.24
Agrimonia asiatica Ius (R) 9.33±0.15* 13.6±0.42
Xantium strumarium h.(R) 9.33±2.15* 14.7±0.81
Cortusa turkestanica A. Los (F) 6.65±1.88*.* 11.8±0.3
Chamenerium angustibolium (L) Scop (R) 7.16±2.11* 18.3±0.95 Stachyopsys oblongata M. Popet Vved (O) 8.63±2.84* 12.4±0.79 Stachys betoniciflora Rupr (F) 11.37±2.17* 17.1±1.24 Ziziphora pedicillate Parii et Vved (St) 17.58±2.28** 18.9±0.29*
Spiraea pilosa Franch (O) 12.63±2.47* 17.0±1.23
Acroptilon repens (R) 10.10±2.11* 18.4±0.45*
Solonum nigra h. (R) 5.79±1.01*.** 12.5±0.17
Phragmites communis Trin (St) 15.2±0.66 17.6±0.79
Cortusa turkestanica A. hos (R) 14.2±2.92 18.8±0.97* Trigonella pamirica Boriss (A) 20.74±1.11*.** 13.4±0.48
Astragalus sp. (R) 13.4±2.11 15.5±0.57
Trifolium pratense L. (R) 15.72±2.54 18.7±0.49*
Solonum nigra h. (L) 15.07±2.24 14.3±0.74
Pulicaria salviibolia Binge (St) 7.09±1.26* 14.7±0.85 Phragmites communis Tren (L) 5.64±1.19*.** 16.3±0.23
Phragmites communis Trin (R) 8.83±1.85* 12.4±0.43
Oberna beechen (L) Ikonn (Fr) 7.38±2.02* 12.4±0.75
Agrimonia ariatica Ius (L) 16.69±2.45** 18.3±0.13*
Serophularia umbrosa Dumort (St) 8.25±2.2* 11.9±0.47 Serophularia umbrosa Dumort (O) 7.86±1.93* 10.9±0.49
Siderities montana L (St) 12.21±1.66* 18.0±0.12*
Phlomis olgae Rgl (R) 16.54±1.87** 17.1±0.18
Serratula alatavica c.A.M. (F) 7.62±3.53* 16.3±0.94
Serratula alatavica c.A.M.(O) 12.36±1.59* 12.4±0.17
Inula helenium L (F) 12.12±1.75* 13.1±0.74
Psoralea drupacea Bunge (R) 12.11±1.96* 12.4±0.19
Inula helenium L (L) 5.82±1.73*.** 17.3±0.89
Phlomis sewerzowii Rgl (O) 11.24±3.41* 16.6±0.62
Note: F - flower; R- root; S - seed; FR - fruit; L - leaves; St - stem; O - overground part; Sk - skin; E - entire plant; I - inflorescence; A - upper part of overground part. Authentic difference Р<0,05: * - from
intact group; ** - from controlled group.
Initial screening in the vegetable extracts found that the most expressed hyperlipidemic
and antioxidative effect were observed in the following extracts: leaves of Juniperus
turkestanica Kom, aboveground part of Acantholimon erythraeum Bge, fruit of
Descurania Sophia (L) Schur, aboveground part of Trigonella pamirica Boriss, seeds of
Avena sativa, roots of Phlomis olgae Rgl. When injecting the extracts from Juniperus
turkestanica Kom, the decrease was observed in concentration of OChS and TG in blood
serum by 45.7 and 30.1% in comparison with the control value of this parameter. With
this process the content ChS HDL increased by 19.1%. Under influence on this extract
the drop of OL for 1.4 times was observed and the increase in content of CP for 15%.
Extract from the leaves of Juniperus turkestanica Kom practically did not affect on the
level of PHE.
Extract which was elicited from the aboveground part of Acantholimon erythraeum Bge
was similar to Juniperus turkestanica Kom in terms of the influence.
Extracts of Avena sativa seeds and roots of Phlomis olgae Rgl supported significant
decrease in OL, OChS, TG, as well as increase in content of Ch HDL and CP in blood
serum of experimental animals. The changes in PHE were statistically insignificant.
No other extracts either revealed hyperlipidemic, antioxidational and membrane stabilizer
effects or showed sufficient effectiveness, or were effective in all the studied parameters.
Primary screening of the 106 extracts for presence of hyperlipidemic, antioxidational and
membrane stabilizer effects under ethanol hyperlipidemia showed that on given models of
hyperlipidemia there are also changes in lipidic blood spectrum (Table 3). Ethanol
hyperlipidemia brings to the increase in OChS and TG by 75.6 and 72.1%, respectively in
comparison with indices of the intact animals group. The content of ChS HDL reduced
by 39.1% and made up 0.28 ± 0.04 mmol/l, in contrast to 0.46 ± 0.9 mol/l in norm. The
given model led to the increase in OL concentration in the blood serum by 49.6%, which
accounted for 6.3 ± 0.43 g/l, whereas the indices of intact animal analogues came up to
4.21 ± 0.36 g/l.
T
ABLE3.
T
HE SCREENING OF VEGETABLE EXTRACTS ON PRESENCE OF HYPOLIPIDEMIC EFFECT UNDER ETHANOL HYPERLIPIDEMIAName of Extracts ОСhS, mmol/l TG, mmol/l СhS HDL, mmol/l OL, g/l
1 2 3 4 5
Intact group 0.99±0.07 0.68±0.09 0.46±0.9 4.21±0.36
Controlled group 1.74±0.1 1.17±0.16 0.28±0.04 6.3±0.43
Plantago lanceolata ® 1.96±0.28* 0.94±0.1* 0.28±0.02* 10.47±0.86*
Rosa canina (Sk) 1.85±0.11* 0.92±0.09* 0.23±0.03* 6.94±1.17*
Cratalgus songarica ® 1.75±0.05* 1.07±0.02* 0.28±0.02* 8.26±0.5*
Cynodon dactulon ® 1.83±0.16* 0.89±0.07 0.29±0.02* 8.9±1.59*
Prunus sogdiana ® 1.72±0.15* 0.98±0.11* 0.25±0.04* 7.22±0.57*
Rosa canina ® 1.91±0.11* 0.9±0.06* 0.27±0.04* 7.4±0.49*
Crataegus pontica (L) 1.78±0.15* 1.04±0.04* 0.25±0.03* 8.4±1.74* Iuniperus zeravshanica (Fr) 1.55±0.2* 1.07±0.19* 0.26±0.04* 12.37±3.48* Cynodon dactulon (O) 1.69±0.15* 0.94±0.09* 0.24±0.03* 7.84±0.52*
Rosa canina (Fr) 1.8±0.03* 0.94±0.1* 0.19±0.04* 5.97±1.03
Picnomon acarna (O) 1.65±0.12* 1.03 ±0.2* 0.2±0.03* 5.7±0.89
Alhagi pseudalhagi ® 1.84±0.1* 0.97±0.1* 0.26±0.02* 7.8±0.25*
Rosa canina (L) 1.96±0.2* 0.95±0.1* 0.32±0.05 7.01±0.92*
Prunus sogdiana (Fr) 1.78±0.2* 0.96±0.2* 0.24±0.07* 5.77±0.41
Chenopodium album (Fr) 1.84±0.1* 0.98±0.2* 0.26±0.06* 5.94±0.74
Rubus caesius (L) 1.69±0.2* 0.96±0.1* 0.25±0.09* 6.18±0.87*
Chenopodium album (R) 1.91±0.1* 0.98±0.15* 0.32±0.1 7.13±0.24* Amaranthus retroplexus (L) 1.75±0.1* 0.78±0.1 0.24±0.06 5.36±0.85 Prunus sogdiana (L) 1.66±0.04* 0.98±0.1* 0.29±0.08* 6.01±0.55* Amaranthus retroplexus (St) 1.38±0.17* 0.85±0.2 0.25±0.06* 6.29±1.1* Amaranthus retroplexus (Fr) 1.89±0.1* 0.94±0.1* 0.28±0.05* 7.33±0.2*
Prunus sogdiana (Sk) 1.8±0.2* 0.98±0.1* 0.2±0.02* 7.34±0.6*
Prunus sogdiana (R) 1.79±0.2* 1.07±0.1* 0.34±0.06 7.06±0.69*
Prunus sogdiana (O) 1.69±0.15* 1.02±0.1* 0.33±0.06 9.0±0.23*
Pheum Fedtshenokovi (F) 1.55±0.3* 0.86±0.075 0.2±0.05 9.6±0.57* Atraphaxis pyrifolia (Sk) 1.63±0.2* 1.0±0.1* 0.3±0.04 9.9±1.13* Atraphaxis pyrifolia (St) 1.89±0.1* 1.04±0.1* 0.24±0.08* 8.4±1.69* Atraphaxis pyrifolia (R) 1.73±0.1* 0.99±0.15* 0.29±0.08* 10.1±0.67* Amygdalis spinosissima (S) 1.9±0.2* 1.04±0.1* 0.3±0.04 10.3±0.38* Cerasus erythrocapra (L) 1.8±0.01* 1.13±0.2* 0.25±0.09* 9.4±0.91* Avena sativa (S) 0.87±0.089** 0.67±0.05** 0.43±0.02** 4.1±0.13**
Amygdalis bucharica (L) 1.75±0.1* 1.35±0.22* 0.3±0.1 7.9±1.2*
Pheum Fedtshenokovi (L) 1.91±0.1* 0.8±0.07 0.24±0.01* 9.3±0.43* Amygdalis bucharica (L) 1.7±0.1* 1.12±0.06* 0.21±0.04* 10.6±0.55*
Trifolium repens (O) 1.79±0.2* 0.93±0.12* 0.2±0.05* 8.4±1.17*
Solidago dahurica Kitag (O) 1.9±0.11* 1.05±0.2* 0.19±0.01* 10.8±1.29* Acroptilon repens ( L)Op.(O) 1.84±0.16* 1.12±0.1* 0.22±0.034* 10.5±0.89* Melandrium turkestanicus (Rgl)Vved (R) 1.9±0.04* 0.79±0.17 0.2±0.029* 10.1±1.74*
Inula helenium (R) 1.78±0.15* 1.0±0.2* 0.28±0.06* 11.1±0.63*
Artemisia vulgaris h. (O) 1.95±0.11* 1.02±0.1* 0.24±0.06* 10.4±0.44* Berberis integerrima (Bge) (Fr) 1.74±0.12* 0.96±0.2* 0.17±0.04* 9.5±1.39* Juniperus turkestanica Kom (L) 1.48±0.06* 0.97±0.1* 0.27±0.02* 5.63±0.3 Solonum nigra h. (Fr) 1.75±0.05* 0.91±0.06* 0.37±0.04 10.4±1.01* Solidago dahurica kitag ® 1.96±0.28* 0.89±0.1* 0.19±0.02* 10.6±0.61* Melandrium turkestanicus (Rgl)Vved (O) 1.95±0.11* 0.84±0.06* 0.28±0.06* 11.0±1.21* Acroptilon repens (F) 1.86±0.12* 0.91±0.1* 0.27±0.03* 9.1±1.19* Trifolim pratense h.(F) 1.72±0.1* 0.98±0.1* 0.26±0.077* 10.5±1.26* Cousinia saawertziwii Regel (O) 1.63±0.38* 0.93±0.1* 0.23±0.022* 8.9±0.2* Anaphalis Velutina Krasch (O) 1.65±0.18* 0.91±0.1* 0.28±0.035* 9.9±0.69* Astragalus sp. (O) 1.73±0.1* 1.07±0.067* 0.19±0.016* 8.6±0.62* Berberis oblonga (Rgl) Schneid ® 1.87±0.03* 0.98±0.1* 0.29±0.032* 8.7±1.59* Hieracium procerum Fr ® 2.0±0.2* 0.9±0.087* 0.27±0.078* 9.7±1.29* Stachys betoniciflora rupr. ® 1.9±0.06* 0.97±0.14* 0.28±0.022* 7.9±0.56* Acantholimon erythraeum Bge (A) 1.73±0.12* 1.1±0.01* 0.28±0.04* 6.1±0.4* Pulicaria salviifolia Bunge (R) 1.73±0.1* 1.02±0.12* 0.31±0.04 9.2±1.15* Psoralea drupacea Bunge (S) 1.7±0.1* 1.01±0.09* 0.18±0.026* 7.4±0.49* Trifolim pratense h.(O) 1.85±0.1* 0.84±0.14 0.25±0.034* 10.5±0.49* Melandrium turkestanicus (Rgl)Vved (Fr) 1.75±0.1* 1.0±0.11* 0.31±0.07* 10.6±0.48* Artemisia vulgaris h. (R) 1.87±0.1* 1.03±0.13* 0.27±0.035* 10.2±1.14* Descurania Sophia (L) Schur (Fr) 1.76±0.27* 1.1±0.16* 0.25±0.026* 6.4±0.65* Xantium strumarium h. (St) 1.78±0.2* 1.08±0.27* 0.23±0.026* 8.9±0.62* Conum maculatum h. (St) 1.75±0.1* 1.03±0.078* 0.21±0.03* 9.9±0.46* Cortusa turkestanica A.Los (O) 1.67±0.05* 0.9±0.082* 0.27±0.067* 10.8±0.68* Tanasetopsis mucronatun S kovalev (O) 1.65±0.07* 0.96±0.09* 0.3±0.075 7.1±0.53* Xantium strumarium h. (S) 1.47±0.32* 0.95±0.04* 0.24±0.038* 7.6±0.53* Herniaria glabra h. (O) 1.45±0.31* 0.81±0.86* 0.21±0.012* 7.8±0.84* Leonurus turkestanicusV.Krecr et Kupr (O) 1.46±0.18* 0.95±0.044* 0.26±0.044* 8.6±0.82* Arctium tomentisum Mill (R) 1.54±0.2* 0.96±0.044* 0.3±0.085 7.7±0.73* Ziziphora pedicillate Parii et Vved (R) 1.86±0.03 0.95±0.11* 0.24±0.045* 7.9±0.55* Solidago dahurica Kitag (F) 1.72±0.15* 0.95±0.05* 0.32±0.071 6.6±0.59* Hierasiun procerum Fr (O) 1.76±0.05* 1.0±0.12* 0.34±0.043 9. 0±0.15* Solonum nigra h. (St) 1.68±0.22* 0.88±0.044 0.3±0.018 8.4±0.63* Agrimonia asiatica Ius (R) 1.5±0.16* 1.02±0.06* 0.23±0.035* 6.8±1.49* Xantium strumarium h.(R) 1.85±0.11* 0.97±0.08* 0.3±0.09 6.5±0.6* Cortusa turkestanica A. Los (F) 1.68±0.23* 1.01±0.08* 0.32±0.07 6.4±0.5* Artemisia vilgaris L.(F) 1.83±0.18* 0.98±0.11* 0.23±0.03* 7.5±1.04* Chamenerium angustibolium (L) Scop (R) 1.63±0.35* 1.04±0.07* 0.3±0.041 7.3±0.23* Stachyopsys oblongata M. Popet Vved (O) 1.73±0.15* 1.04±0.15* 0.26±0.056* 7.0±0.31* Stachys betoniciflora Rupr (F) 1.85±1.33* 0.96±0.057* 0.3±0.075 7.0±0.17* Ziziphora pedicillate Parii et Vved (St) 1.92±0.09* 0.93±0.1* 0. 23±0.018* 5.3±0.075 Spiraea pilosa Franch (O) 1.73±0.16* 0.98±0.052* 0.19±0.018* 6.4±0.87* Acroptilon repens (R) 1.94±0.1* 0.97±0.071* 0.28±0.04* 6.2±0.4* Solonum nigra h. (R) 1.91±0.02* 1.01±0.051* 0.32±0.11 7.4±1.07* Phragmites communis Trin (St) 1.62±0.21* 1.0±0.094* 0.3±0.024 6.9±0.29* Cortusa turkestanica A. hos (R) 1.53±0.31* 1.1±0.053* 0.21±0.057* 8.0±0.46* Trigonella pamirica Boriss (A) 1.58±0.01* 0.96±0.054* 0.24±0.075* 6.9±0.76*
Astragalus sp. (R) 1.62±0.21* 0.96±0.14* 0.31±0.029 7.2±0.55*
Phragmites communis Trin (R) 1.76±0.7* 1.03±0.2* 0.31±0.1 10.6±1.1* Oberna beechen (L) Ikonn (Fr) 1.81±0.22* 1.18±0.15* 0.32±0.02 9.8±1.0* Agrimonia ariatica Ius (L) 1.74±0.12* 0.9±0.1* 0.31±0.1 8.43±1.0* Serophularia umbrosa Dumort (St) 1.78±0.17* 1.05±0.13 0.3±0.04 8.63±0.6* Serophularia umbrosa Dumort (O) 1.92±0.17* 0.98±0.34* 0.3±0.07 9.87±0.8* Siderities montana L (St) 1.85±0.13* 0.85±0.05 0.25±0.04* 8.43±1.0* Phlomis olgae Rgl (R) 1.86±0.05* 1.11±0.09* 0.36±0.035 10.17±1.0* Serratula alatavica c.A.M. (F) 1.89±0.14* 0.99±0.1* 0.3±0.03* 8.41±1.6* Serratula alatavica c.A.M.(O) 1.81±0.12* 0.89±0.1 0.23±0.04* 10.14±1.13*
Inula helenium L (F) 1.91±0.24* 0.77±0.1 0.28±0.01* 11.3±0.6*
Psoralea drupacea Bunge (R) 1.86±0.16* 1.01±0.1* 0.25±0.04* 8.9±1.1* Inula helenium L (L) 1.89±0.09* 0.92±0.14* 0.29±0.05* 8.1±0.8* Phlomis sewerzowii Rgl (O) 1.8±0.35* 0.97±0.06* 0.3±0.08 8.3±0.9* Note: F - flower; R- root; S - seed; FR - fruit; L - leaves; St - stem; O - overground part; Sk - skin; E - entire plant; I - inflorescence; A - upper part of overground part. Authentic difference Р<0,05: * - from intact group; ** - from controlled
group.
Ethanol hyperlipidemia brought to the decrease in the content of CP by 35.6% in
comparison with normal indices. Thus, the level of CP in blood serum in intact animal
and controlled group accounted for 16 ± 0.66 and 10.3 ± 0.9 mg/dl, respectively. Under
ethanol hyperlipidemia there was insignificant rise in PHE: in the group of intact animal
indices running up to 11.3 ± 0.8%, and in the control group / 17.1 ± 0.8%.
The conducting primary screening on model of ethanol hyperlipidemia showed that not all
previously educed active extracts under endogenous hyperlipidemia possess identical
hyperlipidemic quality. Thus, the extract of Juniperus turkestanica Kom leaves had a
strong hyperlipidemic effect on endogenous hyperlipidemia, whilst on ethanol
hyperlipidemia there was a tendency to decrease in OChS, TG and OL; practically there
was not effect on ChS HDL. Under the influence of this particular extract the increase in
CP level by 54.5% was observed as well as an insignificant decrease of PHE in
comparison with the control (Table 4). The extracts which were taken from overground
part of Acantholimon erythraeum Bge, fruit of Descurania Sophia (L) Schur practically did
not effect on the spectrum of blood. However, when injecting these extracts to animals
there was significant rise in CP in blood but no effect on PHE. Extract from aboveground
part of Trigonella pamirica Boriss had weak hypolipidic effect under ethanol
hyperlipidemia. So, the decrease in OChS, TG and OL content was statistically
insignificant under influence of this extract.
T
ABLE4.
T
HE SCREENING OF VEGETABLE EXTRACTS ON PRESENCE OF ANTIOXIDANT AND MEMBRANE STABILIZER EFFECT UNDER ETHANOL HYPERLIPIDEMIAName of Extracts CP, mg/100 ml PHE, %
1 2 3
Intact group 16.0±0.66 11.3±0.8
Controlled group 10.3±0.9 17.1±0.8
Plantago lanceolata (R) 14.1±3.13 14.7±0.71
Rosa canina (Sk) 8.24±1.18* 16.7±0.5*
Cratalgus songarica (R) 6.36±1.31* 14.9±1.22*
Cynodon dactulon (R) 6.31±2.17* 16.1±0.5*
Prunus sogdiana (R) 8.84±2.65* 15.0±1.3*
Rosa canina (R) 8.63±2.84* 15.4±1.44*
Crataegus pontica (L) 5.79±1.01* 15.6±1.22*
Luniperus zeravshanica (Fr) 7.8±0.69* 18.0±1.1*
Cynodon dactulon (O) 6.65±1.88* 15.7±1.6*
Rosa canina (Fr) 6.1±1.14* 17.1±1.5*
Picnomon acarna (O) 8.43±0.87* 12.8±1.3**
Alhagi pseudalhagi (R) 8.1±2.2* 13.3±1.4
Rosa canina (L) 12.12±1.75* 12.0±3.3**
Chenopodium album (Fr) 8.81±1.8* 15.2±1.2*
Rubus caesius (L) 7.86±1.9* 14.1±1.91
Chenopodium album (R) 6.5±0.88* 13.2±1.07
Amaranthus retroplexus (L) 7.6±1.65* 15.6±2.36*
Prunus sogdiana (L) 5.36±0.6* 14.3±2.0
Amaranthus retroplexus (St) 10.9±1.4* 14.6±2.9
Amaranthus retroplexus (Fr) 7.8±1.9* 14.4±1.9
Prunus sogdiana (Sk) 9.7±1.08* 15.0±2.2*
Prunus sogdiana (R) 6.5±1.16* 15.0±1.47*
Prunus sogdiana (O) 9.4±2.28* 14.7±0.87
Pheum Fedtshenokovi (F) 10.9±2.1* 13.4±0.55
Atraphaxis pyrifolia (Sk) 15.3±0.56** 14.5±1.45
Atraphaxis pyrifolia (St) 13.6±2.84* 14.5±1.68
Atraphaxis pyrifolia (R) 15.9±1.88** 14.5±0.3
Amygdalis spinosissima (S) 12.3±2.05* 16.6±0.87*
Cerasus erythrocapra (L) 5.5±0.81* 15.4±0.41*
Avena sativa (S) 17.0±0.7** 11.1±1.2**
Amygdalis bucharica (L) 10.8±1.8* 14.1±1.35
Trifolium pratense (F) 16.6±1.5** 15.8±1.04*
Amygdalis bucharica (Sk) 13.9±1.96* 15.8±0.82*
Pheum Fedtshenokovi (L) 6.2±0.67* 16.5±0.43*
Amygdalis bucharica (L) 10.7±0.97* 15.9±0.81*
Trifolium repens (O) 9.1±2.36* 14.7±0.75
Solidago dahurica Kitag (O) 6.9±1.22* 15.7±2.44*
Acroptilon repens ( L)Op.(O) 5.3±0.9* 13.5±1.87
Melandrium turkestanicus (Rgl)Vved (R) 7.9±1.46* 13.3±1.06
Inula helenium (R) 4.6±1.59* 14.1±0.83
Artemisia vulgaris h. (O) 7.2±1.46* 14.1±0.87
Berberis integerrima (Bge) (Fr) 7.7±0.7* 16.3±1.06* Juniperus turkestanica Kom (L) 15.9±0.8** 15.4±0.88*
Solonum nigra h. (Fr) 5.3±1.51* 15.7±1.11*
Solidago dahurica kitag ® 6.98±1.61* 15.2±2.21*
Melandrium turkestanicus (Rgl)Vved (O) 10.1±1.8* 15.6±0.5*
Acroptilon repens (F) 7.2±2.61* 13.6±0.38
Trifolim pratense h.(F) 6.8±0.84* 17.7±0.93*
Cousinia saawertziwii Regel (O) 5.6±1.46* 11.0±0.75**
Anaphalis Velutina Krasch (O) 8.4±2.11* 14.8±1.31
Astragalus sp. (O) 8.9±1.77* 16.2±1.16*
Berberis oblonga (Rgl) Schneid ® 10.7±0.95* 14.8±2.18
Hieracium procerum Fr ® 8.6±2.05* 16.2±0.97*
Stachys betoniciflora rupr. ® 6.8±1.16* 15.6±0.85* Acantholimon erythraeum Bge (A) 15.6±0.9** 17.4±0.45* Pulicaria salviifolia Bunge (R) 10.7±1.72* 13.9±1.07
Psoralea drupacea Bunge (S) 11.6±2.97* 15.1±2.23*
Trifolim pratense h.(O) 9.4±0.63* 16.1±1.33*
Melandrium turkestanicus (Rgl)Vved (Fr) 6.4±2.4* 15.0±2.26*
Artemisia vulgaris h. (R) 9.4±2.4* 16.0±0.91*
Descurania Sophia (L) Schur (Fr) 15.9±1.23** 16.3±0.71*
Xantium strumarium h. (St) 8.6±0.33* 14.9±1.62
Conum maculatum h. (St) 9.8±2.02* 17.7±3.25*
Cortusa turkestanica A.Los (O) 6.8±1.22* 12.9±0.75** Tanasetopsis mucronatun S kovalev (O) 10.8±1.59* 14.9±1.19
Xantium strumarium h. (S) 9.6±1.29* 13.6±0.76
Herniaria glabra h. (O) 12.9±1.99* 13.5±0.85
Leonurus turkestanicusV.Krecr et Kupr (O) 12.4±3.76* 14.2±1.91
Arctium tomentisum Mill (R) 8.3±2.11* 15.6±1.14*
Ziziphora pedicillate Parii et Vved (R) 9.9±1.53* 11.9±0.53**
Solidago dahurica Kitag (F) 7.4±1.44* 14.4±2.02
Hierasiun procerum Fr (O) 12.1±2.65* 13.5±1.04
Agrimonia asiatica Ius (R) 5.9±2.09* 14.2±1.69
Xantium strumarium h.(R) 12.3±2.25* 16.6±0.53*
Cortusa turkestanica A. Los (F) 7.7±1.36* 16.1±1.07*
Artemisia vilgaris L.(F) 12.4±2.45* 14.9±0.64
Chamenerium angustibolium (L) Scop (R) 8.5±1.75* 12.3±0.7** Stachyopsys oblongata M. Popet Vved (O) 13.6±1.15 14.5±1.65 Stachys betoniciflora Rupr (F) 11.13±2.2* 13.7±0.98 Ziziphora pedicillate Parii et Vved (St) 12.2±1.66* 16.6±1.73*
Spiraea pilosa Franch (O) 11.8±1.44* 15.3±1.28*
Acroptilon repens (R) 11.6±0.82* 17.4±1.12*
Solonum nigra h. (R) 7.2±3.43* 14.2±1.64
Phragmites communis Trin (St) 6.3±1.4* 15.3±2.03*
Cortusa turkestanica A. hos (R) 10.9±1.16* 16.7±1.54* Trigonella pamirica Boriss (A) 11.0±2.31* 13.1±2.35
Astragalus sp. (R) 10.6±0.75* 14.2±1.95
Trifolium pratense L. (R) 6.2±0.39* 17.1±1.12*
Solonum nigra h. (L) 11.0±0.56* 16.3±2.66*
Pulicaria salviibolia Binge (St) 12.5±2.0* 16.3±1.05*
Phragmites communis Tren (L) 10.8±1.43* 14.3±0.37
Phragmites communis Trin (R) 13.5±2.9 16.5±1.65*
Oberna beechen (L) Ikonn (Fr) 9.4±2.2* 14.8±1.42
Agrimonia ariatica Ius (L) 6.8±2.0* 15.8±2.15*
Serophularia umbrosa Dumort (St) 13.5±2.6 16.0±2.38* Serophularia umbrosa Dumort (O) 11.9±2.12* 13.6±0.89
Siderities montana L (St) 12.7±3.3 15.1±2.35*
Phlomis olgae Rgl (R) 16.3±2.04** 16.4±1.69*
Serratula alatavica c.A.M. (F) 10.2±2.3* 14.8±1.35
Serratula alatavica c.A.M.(O) 12.3±3.64* 15.1±1.0*
Inula helenium L (F) 11.3±1.47* 15.1±2.5*
Psoralea drupacea Bunge (R) 9.8±1.68* 14.2±1.47
Inula helenium L (L) 12.8±1.5* 14.1±1.0
Phlomis sewerzowii Rgl (O) 12.7±4.89* 14.1±1.03
Note: F - flower; R- root; S - seed; FR - fruit; L - leaves; St - stem; O - overground part; Sk - skin; E - entire plant; I - inflorescence; A - upper part of overground part. Authentic difference Р<0,05: * -
from intact group; ** - from controlled group
From all the previously revealed active extracts, the seeds of Avena sativa had the most
marked hypolipidic, antioxidative and membrane stabilizer qualities. Researches of this
extract showed that under its influence the lipidic spectrum of the blood normalizes the
indices of antioxidant system and PHE improves. From the results of initial screening of
vegetable extracts in two models of hyperlipidemia, the most perspective extract Avena
sativa was chosen for further examination for its hypolipidic and antiatherosclerotic
features.
Akopov, I., 1990. Important domestic herbal medicine and their application [Vazhnejshie otechestvennie lekarstvennie rasteniya i ih primenenie], in Russian, Tashkent: Medicine..
Аripov, A., Fesenko, L., 2000. Clinical Biochemistry. Methods [Kilicheskay biohimia. Metodi], in Russian, Medical Literature Publishing House named after Abu Ali ibn Sino, Uzbekistan, Tashkent.
Devidenkova, E., Shafran, M., 1998. “Atherosclerosis and lipid peroxidation process,” Bulletin АМN USSR [Vestnik AMN SSSR], in Russian, No.3, pp.10/13.
Nasirov, Sh., Sobirov, D., 2005. “Ischemic heart disease (pathogeny, diagnostics и treatment): Science and technology”, Republic of Uzbekistan, Tashkent.
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