Catarina Isabel Pereira Nunes, PhD student

1 Curriculum Vitae

Catarina Isabel Pereira Nunes, PhD student

Departamento de Química

Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa. Monte da Caparica 2829-516

Caparica, Portugal.

Cell Phone: 00351 92 530 509 3 E-mail: [email protected]

Education:

2005-2009 Degree course in Pharmacy at Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, Lisboa, Portugal

Research Experience:

2011-2013: Team member Project CAPES/FCT 2011-2013: “Genomic and transcriptomic prospection of Exiguobacterium antarcticum. Biotechnological Potential: New proteins related to cold adaptation”.

2010-present: PhD. Dissertation work: Subject: “Arsenic detoxification mechanism in

Desulfovibrio desulfuricans G20”. Advisor: Prof. Dr. Isabel Maria Andrade Martins

Galhardas de Moura, PhD., and Dr. Marta Sofia Peixe Carepo, Ph.D., Chemistry Department, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.

2009-present: Team member REQUIMTE – Chemistry and Tecnology Network, Laboratório Associado para a Química Verde – Tecnologias e Processos Limpos.

2009-2013: Invited Assistant Professor (Pharmaceutical Biotechnology and Praticle Techniques in Pharmacy) at Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, Lisboa, Portugal.

2009: Final Degree Thesis work: Subject: “Arsenate Reductase – Structural and Funtional Biochemistry”. Advisor: Dr. Marta Sofia Peixe Carepo, Ph.D., Chemistry Departament, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal. 2008: Member of organization committee of the degree course. Subject: "The elderly and Medicines", promoted by the students of 3rd year of the degree course in Pharmacy at Escola Superior de Tecnologia da Saúde de Lisboa in partnership with the Câmara Municipal de Loures, Lisboa, Portugal.

2

Laboratory and Informatic Skills:

Experience in most of the basic molecular biology and microbiology methods, such as PCR, DNA extraction and purification, DNA electrophoresis, cloning, protein expression and purification, analytical biochemistry and bacteria culture (aerobic and anaerobic). Also have experience in bioinformatics analysis and genome annotation (Artemis).

Area of scientific activity - Mechanisms of resistance to heavy metals in sulphate reducing bacteria with potential biotechnological application. Biochemical, spectroscopic and functional characterization of proteins involved in arsenic detoxification. Study of the regulation mechanisms of arsenic resistance operons as well as of operons involved in arsenic methylation.

Study Scholarships:

• Graduate Student Scholarships Awarded:

February 2010 to 2014: Fundação para a Ciência e Tecnologia (FCT) PhD fellow (SFRH/BD/62051/2009).

Prizes and Awards:

January 2010: Best Student of degree course of Pharmacy from the Higher School of Health Technology of Lisbon, integrated in the Polytechnic Institute of Lisbon, in 2009/2010 financed by Caixa Geral de Depósitos.

June 2005: School Merit Certificate, Escola Secundária de Bocage, Setúbal, Portugal.

Main Publications:

• Peer-reviewed Journals

December 2012: Carneiro AR, Ramos RT, Dall’agnol H, Pinto AC, de Castro Soares S, Santos AR, Guimarães LC, Almeida SS, Baraúna RA, das Graças DA, Franco LC, Ali A, Hassan SS, Nunes CI, Barbosa MS, Fiaux KK, Aburjaile FF, Barbosa EG, Bakhtiar SM, Vilela D, Nóbrega F, Dos Santos AL, Carepo MS, Azevedo V, Schneider MP, Pellizari VH, Silva A. Genome Sequence of Exiguobacterium antarcticum B7, Isolated from a Biofilm in Ginger Lake, King George Island, Antarctica. J. Bacteriol. 194(23-9):6689-90.

• Final Degree Thesis

September 2009: Thesis to achieve the Degree in Pharmacy from the Higher School of Health Technology of Lisbon, integrated in the Polytechnic Institute of Lisbon. Supervisor: Dr. Marta Sofia Peixe Carepo, Ph.D. Title: “Arsenate Reductase – Structural and Funtional Biochemistry”.

3 • Conference proceedings:

Internacional meetings:

June 2013: 5th Chemistry for Life Science Conference, Barcelona, Spain. Poster presentation: “Proteins involved in heavy metal resistance (Mo and As) in Sulfate-reducing bacteria”. N.S.S. Castro, C.I.P. Nunes, M. Raposo, C.A. Laia, M. Ansaldi, J.J.G. Moura, I. Moura, M.S.P. Carepo.

September 2012: EUROBIC 11, Granada, Spain. Poster presentation: “Arsenate reductases in Desulfovibrio desulfuricans G20”. C.I.P. Nunes, J.J.G. Moura, I. Moura, M.S.P. Carepo. April 2012: International Polar Year 2012 Conference, at Montréal, Canada. Poster

presentation: “Proteomics of the psychrotrophic bacterium Exiguobacterium antarcticum

B7”. R. A. Baraúna, F. N. Pereira, H. P. M. Barbosa, A.R. Carneiro, C.I.P. Nunes, M.S.P. Carepo, V.H. Pellizari, M.P.C. Schneider, A. Silva.

April 2012: International Polar Year 2012 Conference, at Montréal, Canada. Poster

presentation: “Analysis of the genomic architecture of the psychrotrophic bacteria

Exiguobacterium antarcticum B7 strain to in silico reconstruction of the metabolic

pathways of adaptation cold”. A.R. Carneiro, R.A. Barauna, H.P.M. Barbosa, F. Nóbrega, C.I.P. Nunes, M.S.P. Carepo, V.H. Pellizari, M.P.C. Schneider, A. Silva.

Abril 2012: International Polar Year 2012 Conference, at Montréal, Canada. Poster

presentation: “Microbial thermal adaptation revealed by transcriptomic analysis of the

psychrotrophic bacterium Exiguobacterium antarcticum B7”. H.P.M. Barbosa, A. Carneiro, R.A. Baraúna, F. Pereira, P.H. Sá, C.I.P. Nunes, M.S.P. Carepo, M.P.C. Schneider, V.H. Pellizari, A. Silva.

June 2011: 36th FEBS Congress “Biochemistry for Tomorrow´s Medicine”, at Turin, Italy. Poster presentation: “Arsenic Detoxification in Desulfovibrio desulfuricans G20” C.I.P Nunes, J.J.G. Moura, I. Moura, M.S.P Carepo.

May 2011: Combined Practical and Lecture Course “Chemistry of Metals in Biological Systems” by Federation of European Biochemical Societies (FEBS), at Louvain-la-Neuve, Belgium. Poster presentation: “Arsenic Resistance in Desulfovibrio desulfuricans G20” C.I.P Nunes, J.J.G. Moura, I. Moura, M.S.P Carepo.

National meetings:

January 2011: REQUIMTE BIENNIAL MEETING FÁTIMA 2011, at Fátima, Portugal. Poster presentation: “Bacterial Stress Responses”. Nathália Castro, Cristiano S. Mota, Luís M. Nobre, Catarina Nunes, Cecilia Pinto, Isabel R. Fernandes, Iris L. Ziober, Rashmi Nair, Biplab K. Maiti, Cristina Costa, Raquel Grazina, Maria Gabriela Rivas, Marta S. P. Carepo, Sofia R. Pauleta, Isabel Moura.

June 2010: International School in Sustainable Chemistry at Caparica, Portugal. Poster presentation: “Importance of Thioredoxin in Anaerobic Systems”. C. Nunes; I. Moura, M. Carepo.

4 2008: XXII National Meeting of the Pharmacy Technicians at Faro, Portugal. Poster presentation “Medicinal Plants - Weight-Lost Therapy”. Nunes, C; Farinha, F; Coelho, A; Gato, S; Graça, A.

Courses Attended

Combined Practical and Lecture Course “Chemistry of Metals in Biological Systems”. Federation of European Biochemical Societies (FEBS), Louvain-la-Neuve, Belgium. 15-22/05/2011.

Online Course in Nanotechnology for Health – Applications in Laboratory Diagnosis. Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, Lisboa, Portugal. 18/12/2010 – 18/02/2011.

2nd Short Course on Electrochemical Biosensors. Chemistry Department, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal. 20-22/10/2010. 5th Short Course on Electrochemistry of Proteins. Chemistry Department, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal. 18-20/10/2010. 9th Short Course of the Portuguese Biophysical Society – Protein folding, misfolding and disease. Santarém, Portugal. 9-10/10/2010.

International School in Sustainable Chemistry, PhD. Program in Sustainable Chemistry. Chemistry Department, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal. 28/06-2/07/2010.

Entrepreneurship School, PhD. Program in Sustainable Chemistry. Faculdade de Farmácia, Universidade do Porto, Porto, Portugal. 12-16/04/2010.

School in Scientific Communication, PhD. Program in Sustainable Chemistry. Chemistry Department, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal. 18-22/01/2010.

Language Skills

• English: Speak, write and read fluently.

References

Dr. Marta Sofia Peixe Carepo, Ph.D.

BioIn & BioProt– Grupo de Bioinorgânica e Bioquímica Física de Proteínas Departamento de Química

5 Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa.

2829-516 Monte da Caparica Caparica, Portugal.

[email protected]

Dr. Isabel Maria Andrade Martins Galhardas de Moura, Ph.D. Professor BioProt – Grupo de Bioquímica Física de Proteínas

Departamento de Química

Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa. 2829-516 Monte da Caparica

Caparica, Portugal. [email protected]