Arcobacter butzleri and A. cryaerophilus in human, animals and food sources, in southern Chile

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Arcobacter butzleri and A. cryaerophilus in human, animals and food sources,

in southern Chile

Heriberto Fernandez

1

, Maria Paz Villanueva

1

, Ingrid Mansilla

2

,

Mario Gonzalez

1

, Fadua Latif

1

Instituto de Microbiologia Clinica, Universidad Austral de Chile, Valdivia, Chile. 2

Instituto de Salud Pública de Chile, Santiago, Chile

Submitted: February 1, 2014; Approved: June 6, 2014.

Abstract

The isolation frequency of Arcobacter species in children with diarrhea, fowls, mammals and food of avian and marine origin was established. In all the samples it was possible to isolate Arcobacter spe-cies corresponding 201 (39.4%) to A. butzleri and 24 (4.7) to A. cryaerophilus. Both spespe-cies were si-multaneously isolated in 19 (3.7%) being A. butzleri the most frequently isolated species.

Key words: Arcobacter diarrhea, reservoirs, food.

Introduction

Arcobacter sp. belongs to Campylobactereaceae fam-ily as was proposed by Vandamme et al. (1991). This genus included 18 species with A. butzleri, A. cryaerophilus and A. skirrowii been considered the only emerging entero-pathogens of relevance for animals and humans (Lehner et al., 2005; Wesley and Miller, 2010; Collado and Figueras, 2011; Euzéby, 2014). A. skirrowii has been isolated from sheep and cattle with diarrhea, aborted pig fetus, and chronic and acute diarrhea in humans. A. cryaerophilus and A.butzleri have been associated with abortion and enteritis in animals as well as with diarrhea and bacteremia in chil-dren and adults. The transmission of these organisms oc-curred by fecal-oral, waterborne, by food of animal origin or by direct contact with animals (Lehner et al., 2005; Wes-ley and Miller, 2010; Collado and Figueras, 2011).

In Chile there is scarce information regarding this group of bacteria. Fernández et al. (2004) reported the first isolates in this country. Due to the clinical relevance of Arcobacter as foodborne pathogen and the need to get more information related to its ecological distribution and preva-lence, we present this communication. We are reporting the prevalence of Arcobacter in children with diarrhea, in ani-mal reservoirs and in food of aniani-mal origin. The study was performed in the south part of Chile (Los Rios Region,

39º16' and 40º41' latitude South and 71º35' longitude West until the Pacific Ocean).

Material and Methods

We studied a total of 510 samples. The type and ori-gin of the samples are described in Table 1. Human feces (approx. 2 g) were inoculated into Arcobacter broth (20 mL) and food samples (approx. 25 g previously vortex) were added into 125 mL of Arcobacter broth (Houf et al., 2001). After 48 h of incubation at 30 °C under aerobic con-ditions, 100 microliters aliquots were streaked onto Arcobacter agar plates (Houf et al., 2001). Additionally, 400 microliters of enrichment broth were filtrated onto blood agar plates using a 0.45 mm filter (Fernández et al., 2004). All the plates were incubated under the same condi-tions described above. Colonies with 2 to 4 mm diameter and smooth borders were tested for Gram stain, wet prepa-ration for contrast microscopy, and oxidase and catalase tests. Those colonies with oxidase and catalase positive of rods gram negative with characteristic motility spinning around their long axes under the contrast microscope were considered presumptively Arcobacter sp.. Those colonies were streaked in blood agar plates for confirmatory testing using multiplex PCR as proposed by Houf et al. (2000) for the confirmation of the three main species of Arcobacter.

ISSN 1678-4405 www.sbmicrobiologia.org.br

DOI: http://dx.doi.org/10.1590/S1517-838246120140095

Send correspondence to H. Fernandez. Instituto de Microbiologia Clinica, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile. E-mail: hfernand@uach.cl.

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Results

As indicated on Table 1, Arcobacter was isolated from all the sample types. A total of 201 (39.4%) strains of A. butzleri, and 24 (4.7%) of A. cryaerophilus were iso-lated. In 19 (3.7%) of the samples studied both species were recovered simultaneously.

From children with diarrhea and from mussels, only A. butzleri was isolated (3.6% and 22.7%, respectively).

In chicken feces, A. cryaerophilus was most fre-quently isolated (20%) than A. butzleri (10%) but in pig and bovine feces occurred the opposite. The frequency of A. butzleri was 40.7% in pigs and 26.7% in bovines while for A. cryaerophylus they were 9.6 and 6.7%, respectively.

High frequencies of Arcobacter were found in poultry meat ready for human consumption (chicken meat 92%, muscular stomach 32%, and liver 92%).

Discussion

A. butzleri has been described as the Arcobacter spe-cies more frequently isolated from clinical and environ-mental specimens, as well as from food of animal origin (Lehner et al., 2005; Wesley and Miller, 2010; Collado and Figueras, 2011), and it was confirmed as the most prevalent specie in this study. A. butzleri was the only Arcobacter specie isolated from children with diarrhea with 3.6% prev-alence. The prevalence of Arcobacter in this study was higher to the prevalence found by Prouzet-Mauleon et al. (2006) in France (1%) and to the prevalence previously re-ported by Collado et al. (2013) in Valdivia, Chile (1.7%). However, the prevalence of Arcobacter in this study was similar to the prevalence observed by Vandenberg et al. (2004) in Belgium (3.5%). Similar to the reports of Prouzet-Mauleon et al. (2006) and Collado et al. (2013), we were unable to recovery A. skirrowii or A. cryaerophilus from feces of children with diarrhea. The only study in which A. cryaerophilus was isolated from feces of children with diarrhea was the report of Vandenberg et al. (2004) but

as in other studies included the present one, A. skirrowii was not recovered.

In Europe, the reports indicated that Arcobacter spe-cies represent the 4thplace in frequency of isolation among the member of the Campylobactereaceae family in samples of fecal origin (Vandenberg et al., 2004; Prouzet-Mauleon et al., 2006). The high prevalence of A. butzleri found in di-arrhea, similarly at is occurs with Campylobacter jejuni/coli, might be an indicator of similar risk factors in the environment (Wesley and Miller, 2010, Fernández, 2011).

In chicken feces, A. butzleri was isolated (10%) as well as A. cryaerophilus (20%). The prevalence found in this study is similar to previous studies (Fernández et al., 2007; Houf, 2010). However, the prevalence of Arcobacter species in pig and bovine feces was lower that the preva-lence of Campylobacter species in the same type of sam-ples (Table 1). The findings in this study were similar to results previously reported in the bovine and pig samples (Fernández, 2011; Fernández et al., 2007; Houf, 2010).

Some investigators have proposed that the low preva-lence of Arcobacter species in chickens is an indicator that this organism is not a natural intestinal commensal in chicken and represent only a transient organism incapable to colonize the gut chicken. A potential explanation for this phenomenon is that the normal corporal temperature in chicken (40.5 to 42 °C) represents a limiting factor for the colonization of chicken with Arcobacter species because their optimal growth temperature range is from 26 to 30°C, contrasting with Campylobacter that is recovered in high prevalence in chicken due to its optimal growth tempera-ture that is around 42°C (Wesley and Miller, 2010; Houf, 2010). However, in contrast from the animal samples, the recovery of Arcobacter from poultry meat ready for human consumption is high (92% in chicken meat, 32% in muscu-lar stomach, and 92% in liver). These results of prevalence of Arcobacter in poultry meat are in agreement with those previously reported (Fernández et al., 2001; Collado and Figueras, 2011). According to Wesley and Miller (2010)

146 Fernandez et al.

Table 1 - Prevalence of Arcobacter species among human, animal and food samples.

Origin Type Number A. butzleri A. cryaerophilus A. butzleri + A. cryaerophilus

N° % N° % N° % Children Feces 83 3 3.6 0 0 0 0 Pig Feces 135 55 40.7 13 9.6 12 8.9 Bovine Feces 75 20 26.7 5 6.7 3 4.0 Chicken Feces 20 2 10.7 4 20.0 0 0 Chicken Meat 125 90 72.0 2 1.6 3 2.4 Chicken Stomach 25 8 32.0 0 0 0 0 Chicken Liver 25 18 72.0 0 0 1 4.0 Seafood Mussels 22 5 22.7 0 0 0 0 Total 510 201 39.4 24 4.7 19 3.7

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and Houf (2010), the explanation for this discrepancy is that poultry may be contaminated in processing plants and the temperature used for storage of the product (4°C and/or room temperature) may favor the colonization by external sources.

The frequency of recovery Arcobacter species was higher in feces from bovine, pigs, and poultry meat sam-ples. Among those samples, A. butzleri and A. cryaerophilus were recovered simultaneously. This ob-servation has been previously reported (Lehner et al., 2005; Collado and Figueras, 2011; Houf, 2010).

The prevalence of A. butzleri in mussels was 22.7%, this prevalence was different from previous reports by Fernandez et al. (2001) and Collado et al. (2009) who re-ported higher prevalence of A. butzleri and the recovery of additional Arcobacter species in the same type of seafood. In summary, this report confirmed the presence of Arcobacter species in children with diarrhea in the south re-gion of Chile. In addition, this report confirmed the pres-ence of Arcobacter in animal reservoirs and in food of animal origin such as poultry meat and seafood. We believe that the presence of Arcobacter may be an indicator of fecal contamination in the human environment and food sup-plies. Therefore, it is imperative to establish a constant sur-vey to determine its environmental distribution, its presence in other animal sources as well as the epidemio-logical relationship between Arcobacter strains isolated from different sources and their mode of transmission. This information will contribute to clarify and a better under-standing of the epidemiology of this emerging entero-pathogen.

Acknowledgments

The study was financially support by projects DID-UACH S-2004-01 and FONDECYT 1110202. Thanks are due to Dr. Guillermo Pérez-Pérez (New York University Langone Medical Center) for their critical review and sci-entific advice.

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Associate Editor: Nilton Erbet Lincopan Huenuman

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