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GAS EXCHANGE OF THE UMBU TREE UNDER SEMI-ARID CONDITIONS
1JOSÉ MOACIR PINHEIRO LIMA FILHO 2
ABSTRACT - A study was carried out at Embrapa Semi-Árido, Petrolina-PE, Brazil, aiming to understand the gas exchange process of the umbu tree
(Spondias tuberosa Arr. Cam.) in the dry and rainy seasons. Stomatal conductance, transpiration, photosynthesis and internal CO2 concentration
were obtained with a portable infrared gas analyzer (IRGA). During the dry season the umbu tree showed a much lower stomatal conductance early
in the morning, as soon as the vapor pressure deficit increased, apparently affecting CO2 assimilation more than transpiration. The highest values were
detected around 6:00 am but decreased to the lowest points between 10:00 am and 2:00 pm. During the rainy season, however, stomatal conductance, transpiration and photosynthesis were significantly higher, reaching the highest values between 8:00 and 10:00 am and the lowest around 2:00 pm. It was also observed at 4:00 pm, mainly during the rainy season, an increase on these variables indicating that the umbu tree exhibits a two-picked daily course of gas exchange.
Index terms: Spondias tuberosa, stomatal conductance, transpiration, photosynthesis, CO2 internal concentration1
TROCAS GASOSAS DO UMBUZEIRO SOB CONDIÇÕES SEMI-ÁRIDAS
RESUMO - Realizou-se um trabalho na Embrapa Semi-Árido, em Petrolina-PE, com o objetivo de estudar as trocas gasosas do umbuzeiro (Spondias
tuberosa Arr. Cam.), durante as estações seca e chuvosa. Os resultados obtidos basearam-se em observações da condutância estomática, transpiração,
fotossíntese e concentração interna de CO2, mensuradas com auxílio de um analisador portátil de gás infravermelho. Durante a estação seca, o
umbuzeiro apresentou baixa condutividade estomática logo no início do dia, decrescendoà medida que aumentou o déficit de pressão de vapor,
aparentemente afetando mais a assimilação de CO2 que a transpiração. Os valores mais altos foram observados em torno de 6 h, decrescendo até atingir
os valores mais baixos entre 10 h e 14 h. Entretanto, durante a estação chuvosa, a condutividade estomática, transpiração e fotossíntese foram significativamente maiores, atingindo os valores mais altos entre 8 h e 10 h, e os mais baixos em torno das 14 h. Constatou-se também, principalmente durante a estação chuvosa, um aumento destas variáveis às 16 h, indicando que o umbuzeiro exibe dois picos diários de trocas gasosas.
Termos para indexação: Spondias tuberosa, condutividade estomática, transpiração, fotossíntese, concentração interna de CO2.
INTRODUCTION
The umbu tree (Spondias tuberosa Arr. Cam.) belongs to the Anacardiceae family which grows naturally in the ¨Caatinga¨ vegetation throughout the semi-arid Northeast Brazil. During the dry season, the plant shed their leaves to avoid transpiration. The flowering process begins in the dry season, when the first leaves appear at the base of the inflorescence. However, leaf flushing is enhanced after the first rains. The survival of the species in such hash environment is assured by a specialized root system bearing tubers, which function is storing water, minerals and other solutes. These resources are used during the dry season for maintaining plant normal metabolism and the beginning of the flowering process. The gathering of fruits is a very important activity for complementing the familiar income of small farmers (Mendes, 1990; Cavalcante et al., 1996).
Due to its social and economic importance, the umbu tree has been studied in relation to dispersion (Santos, 1997), propagation (Oliveira et al., 1989; Nascimento et. al, 1993), morphology (Braga, 1976), root anatomy (Lima, 1994) and fruit chemical composition (Narain et al, 1992). Regarding its physiological behavior and interaction with the environment, very few reports are found in the literature. In this context, Ferri & Laboriau (1952) and Ferri (1953), studied the water balance of this species during the dry season, based on stomatal behavior. They observed that maximum transpiration occurred between 7:00 am and 9:00 am.
Lima Filho & Silva (1988), showed that the umbu tree exerts a rigid control of transpiration through the stomata, even under good soil moisture conditions, assuring a significant water economy. More recently Lima Filho (2001) studied the internal water relations of this species and suggested that under dry condition the diurnal water balance would be maintained at the expenses of water stored in the tubers and by restricted transpiration. During the rainy season, the balance may have been mediated by a short term osmotic adjustment.
In relation to gas exchange, however, nothing was found in the literature suggesting there is a need for insights on this subject. Therefore, our objective was to study the gas exchange of the umbu tree in order to understand its physiological behavior under two distinct environmental
situations naturally experienced by the species.
MATERIAL AND METHODS
The experiment was carried out at the Embrapa Semi-Árido, Petrolina, Pernambuco State, Brazil, at the end of the dry season of 1994 (October) and during the rainy season of 1995 (March), after an accumulated precipitation of 510 mm. Leaf gas exchange was monitored with a portable photosynthesis system (LI-6200 - LICOR, USA), using a ¼ liter assimilation chamber. The study was carried out on four nearby trees, grown under natural conditions and data collected on four sun exposed leaves per tree, located at the canopy midpoint. A completely randomized experimental design with four replicates was used. During the dry season, the trees had already initiated the flowering process when the first leaves appeared at the base of the inflorescence. Photosynthetic active radiation, air temperature and humidity were obtained with respective sensors connected to the LI-1000 datalogger (Licor, USA). Data on temperature and relative humidity were used to calculate the vapor pressure deficit. All data were monitored from 6:00 am to 6:00 pm during three cloudless days, on both seasons. The statistical analysis (ANOVA) was applied to compare the hourly means between seasons for each physiological variable using the SAS software.
RESULTS AND DISCUSSION
The environmental variables recorded during physiological data measurement are presented in Figure 1. During the dry season, the photosynthetic photon flux density was higher in the morning but lower in the afternoon than during the rainy season as a result of differences in solar inclination between seasons. However, the highest value occurred
at noon time, reaching around 2000 µmol m-2s-1, in both seasons.
Despite the small differences in solar radiation, air temperature and vapor pressure deficit were significantly higher during the dry season. Early in the morning, temperature and vapor pressure deficit were,
respectively, 25 oC and 0.59 kPa in the dry and 22.5 oC and 0.36 kPa in the
rainy season .The highest values were observed around 2:00 pm, reaching 1 (Trabalho 149/2003). Recebido: 09/10/2003. Aceito para publicação: 05/08/2204.
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0 4 8 12 16 20 24 T ran sp ir atio n (mmo lm· ² s· ¹) 0 4 8 12 16 Phot os ynt hes is (µ m ol m ·² s· ¹) 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 dry rainy 100 150 200 250 300 350 400 450 6 8 10 12 14 16 18 Day time ( h ) In te rc ellu la r C O2 (ppm ) C onduct ance (m ol m ·² s· ¹) 0 5 10 15 20 25 PP FD (µ mol m ·² s· ¹) x 100 dry rainy 0 10 20 30 40 A ir te m pe ra tu re ( °C ) 0.0 0.5 1.0 1.5 2.0 2.5 Day time ( h ) DP V (KP a) 6 8 10 12 14 16 18
37 oC and 2.35 kPa in the dry and 32 oC and 1.92 kPa in the rainy season.
It is evident that the differences on data observed between seasons or long term changes (Schulze & Hall, 1982), were mostly related to the environmental differences experienced by the plants. Indeed, the statistical data analysis detected that the interaction measurement time x seasons was significant (P<0.01), indicating that the umbu tree behaved differently, within the time of measurement, regarding the distinct environmental conditions studied.
It is known that during the dry season this species maintains a favorable internal water balance at the expenses of water stored in the root tubers (Lima Filho, 2001). This adaptation should buffer the transpiration losses. However, due to the high evaporative demand, the stored water was not enough to keep the stomata wide opened. Thus,
stomatal conductance, transpiration, photosynthesis but internal CO2
concentration were significantly lower and presented different hourly trend in relation to the rainy season (Figure 2).
Early in the morning, stomatal conductance was 0.16 mol m-2s
-1,decreasing to 0.027 mol m-2s-1 as soon as the air temperature and VPD
increased from 25 oC to 31 oC and from 0.59 kPa to 1.14 kPa, respectively,
at 8:00 am, when radiation was below 1,000 µmol m-2s-1 (Figure 1). This
tendency was observed until 2:00 pm when maximum values of solar radiation, air temperature and VPD were detected. At this point,
FIGURE 1- Diurnal fluctuation of photosynthetic active radiation, air
temperature and vapor pressure deficit, observed in the end of the dry season of 1994 and in the rainy season of 1995.
FIGURE 2 - Diurnal courses of stomatal conductance, transpiration,
photosynthesis and intercellular CO2 concentration of the
umbu tree, observed in the end of the dry season of 1994 and in the rainy season of 1995. Bars represent the standard deviation.
conductance reached its lowest value, around 0.01 mol m-2s-1. A slight
recovery to 0.02 mol m-2s-1 was observed at 4:00 pm as a function of the
decreasing evapotranspiratory demand. Stomatal responses to humidity may reflect a mechanism, which prevents extreme rates of water loss and extreme water deficit when plants are subjected to high evaporative demands (Schulze & Hall, 1982).
Because stomatal conductance affects leaf gas exchange, transpiration and photosynthesis, data followed the same hourly trend. GAS EXCHANGE OF THE UMBU TREE UNDER SEMI-ARID CONDITIONS
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Therefore, leaf transpiration reached 3.22 mmol m-2s-1 at 6:00 am, when
stomatal conductance was maximum and decreased to 0.35 mmol m-2s-1
at 2:00 pm (Figure 2). Recovery was also observed at 4:00 pm, when
values were around 1.32 mmol m-2s-1. By the end of the light period,
transpiration was about zero. This pattern was also observed by Lima Filho & Silva (1988).
Although very low, transpiration was sustained in the dry season emphasizing the importance of root tubers as water reservoir (Lima Filho, 2001). Under this condition, leaf photosynthesis seemed to
be more negatively affected than transpiration. In fact, CO2 assimilation
dropped from 3.25 µmol m-2s-1, at 6:00 am, to around 0 µmol m-2s-1, from
10:00 am to 2:00 pm. Apparent recovery happened around 4:00 pm when
photosynthesis reached 0.38 µmol m-2s-1, but it decreased to zero, as
expected, in the end of the day. Because the umbu tree presents a C3 carbon fixation mechanism, it is possible that the high temperature, in addition to severe plant water stress, may have trigged the photorespiratory process and thus, playing a role on reducing assimilation (Taiz & Zeiger, 1991). This situation may have happened
because the intercellular CO2 concentration increased from around 250
ppm, early in the morning, to 370 ppm at 2:00 pm while assimilation
decreased to near 0 µmol m-2s-1 (Figure 2). In this case, part of the CO
2
evolved from respiration remained intercellular due to stomatal closure. In the rainy season the values of stomatal conductance was
about 0.72 mol m-2s-1 and increased to 1.17 mol m-2s-1 at 10:00 am when
temperature and vapor pressure deficit were 26.5 oC and 0.96 kPa,
respectively (Figure 2). From that point it dropped steadily to 0.14 mol m
-2s-1 under 32 oC and 1.92 kPa around 2:00 pm. Recovery was observed at
4:00 pm when 0.47 mol m-2s-1 was recorded.
Transpiration increased from 2.1 mmol m-2s-1 by the beginning
of the light period, to 17.2 mmol m-2s-1 around 10:00 am but it decreased
to 5.3 mmol m-2s-1 at 2:00 pm. It recovered at 4:00 pm to values around
10.7 mmol m-2s-1 but decreased again, as expected, at 6:00 pm. In the
same way, photosynthesis peaked from 1.6 µmol m-2s-1 immediately after
6:00 am, to about 15.0 µmol m-2s-1 at 10:00 am. Furthermore, it dropped
almost linearly to 8.6 µmol m-2s-1 around 2:00 pm. At 4:00 pm it increased
to 10.4 µmol m-2s-1, falling again to 0.6 µmol m-2s-1 by 6:00 pm.
The better performance of the umbu tree regarding assimilation during the wet season can be viewed by the course of the intercellular
CO2 concentration (Figure 2), which is typically 220 ppm in C3 species
(Wong et al. 1979). In the present study, the values observed were close to this number during most part of the day.
The results indicate that during the rainy season the umbu tree showed a two-peaked daily course of stomatal conductance and
consequently of water vapor and CO2 exchange. This pattern may have
been caused by changes in the atmospheric environment during the day (Schulze et al., 1975), such as low air humidity, even when plants were adequately irrigated (Tenhunen et al., 1981).
CONCLUSION
1. The environmental difference between the dry and the rainy seasons is the main factor influencing the course of gas exchange of the umbu tree under semi-arid conditions.
2. In the dry season the umbu tree maintains a very low transpiration and photosynthesis during the whole day due to low levels of stomatal conductance.
3. During the rainy season, transpiration and photosynthesis of the umbu tree are high during most part of the day but the values decrease in the early afternoon as consequence of a transient decrease on stomatal conductance.
4. The umbu tree exhibits a two-picked daily course on gas exchange.
ACKNOWLEDGEMENTS
We are thankful to Dr. Eduardo Caruso Machado for his comments on the manuscript, and to Mr. Genival Nunes Ferreira for his valuable help, during data collection.
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