Biological Resource Center. Fundação Oswaldo Cruz CRB-SAÚDE

(1)

Biological Resource Center for Health

for Health

Fundação Oswaldo Cruz

(2)

The start of BRC at Fiocruz: CLIOC The start of BRC at Fiocruz: CLIOC

May 2005 – Workshop

CRBs and Conformity

Assessment for Biological

May 2005 – Workshop

CRBs and Conformity

Assessment for Biological

In loco audits in the

collections candidates to the accreditation (ISO 17025)

In loco audits in the

collections candidates to the accreditation (ISO 17025) Selection of collections able to accredited by INMETRO Material Material 17025)17025) INMETRO July 2005 - Internal Auditing Training on ISO/17025:2001 July 2005 - Internal Auditing Training on ISO/17025:2001 Establishement of the prototype of Brazilian CRB Network Establishement of the prototype of Brazilian

CRB Network ISO/17025:2001ISO/17025:2001

4 collections were selected:

CLIOC - FIOCRUZ CRB Network CRB Network CLIOC - FIOCRUZ CBMAI - UNICAMP CENARGEN - EMBRAPA Banco de Células - UFRJ Banco de Células - UFRJ

(3)

CLIOC: Model for the BRC at Fiocruz

Activities developed within the BRC project

• Conclusion of the physical infra-structure (space remodelling)

• Establishment of the Quality Management System (NBR ISO 17025)

• Adequacy to the OECD guidelines

• Inventory process

• Definition of the ideal method for preservation and molecular techniques

• Definition of the ideal method for preservation and molecular techniques developed to determine the levels of intra-specific biodiversity

Research

• Microsatelites

• Multi-locus sequencing typing

• Comparative genome

• Taxonomic markers

• Drug resistance markers

• Functional studies: growth curve, gene expression

(4)

CLIOC: Model for the BRC at Fiocruz Year of 2009 (Strains)

CHARACTERIZED DEPOSITED DONNATED

90 156 123

TOTAL = 369

Services offered by CLIOC to

TOTAL = 369

Services offered by CLIOC to

• research

• development of vaccine and diagnostic kits projects

(5)

Bacteria Collections

I. Coleções de Bactérias

Bacteria Collections

1.1. Coleções de Bactérias do IOC:

- Coleção de Culturas de Bactérias de Interesse em Saúde:

- Enteropatógenos Bacterianos - Enteropatógenos Bacterianos - Bactérias de Origem Hospitalar - Gênero Listeria

- Gênero Campylobacter - Gênero Campylobacter

- Coleção de Culturas do Gênero Bacillus e Gêneros Correlatos - Coleção de Bactérias da Mata Atlântica

1.2. Coleções de Bactérias do IAM:

- Coleção de Culturas de Yersinia - Coleção de Bactérias NB2 do IAM - Coleção de Bactérias NB2 do IAM

1.3. Coleção de Microorganismos de Referência do INCQS – Domínio Bactérias

(6)

Fungi Collections Fungi Collections

II. Coleções de Fungos

2.1. Coleções de Fungos do IOC: 2.1. Coleções de Fungos do IOC:

- Coleção de Culturas de Fungos do IOC – CCF/IOC - Coleção Micológica de Trichocomaceae

2.2. Coleção de Microorganismos de Referência do INCQS – Domínio Fungos

2.3. Coleção de Fungos Patogênicos do IPEC 2.3. Coleção de Fungos Patogênicos do IPEC 2.4. Coleção de Fungos da Amazônia do ILMD

(7)

Protozoa and Virus Collections Protozoa and Virus Collections

III. Coleções de Protozoários

3.1. Coleções de Protozoários do IOC: 3.1. Coleções de Protozoários do IOC:

- Coleção de Trypanosoma de Reservatórios Silvestres, Domésticos e Vetores

- Coleção de Leishmania do IOC – CLIOC/IOC - Coleção de Leishmania do IOC – CLIOC/IOC

3.2. Coleções de Protozoários do IGM

IV. Coleções de Vírus

4.1. Banco de Vírus do LAVITE do IAM 4.1. Banco de Vírus do LAVITE do IAM

(8)

Microbial Collections – FINEP/Modernit (2007)

– Coleção de Culturas do Gênero Bacillus e Gêneros Correlatos do IOC

– Coleção de Culturas de Bactérias de Interesse em Saúde do IOC

– Coleção de Culturas de Fungos do IOC

– Coleção Micológica de Trichocomaceae do IOC

– Coleção de Leishmania do IOC

– Coleção de Bactérias da Amazônia do ILMD

(9)

Quality Environmental Project: “Cleaner Research” P+L ↓ energy consumption ↓ energy consumption water consumption water consumption ↓ residues consumption ↓ residues consumption

Quality Environmental Project: “Cleaner Research” P+L

consumption consumption consumption

consumption consumptionconsumption

• Calibration of equipments equipments • Equipment substitution • Sensitization Chemical management, storage Reverse osmosis • Sensitization process to optimize the use of management, storage and discard

Reverse osmosis the use of equipments

• Paper recycling Partnership with

(10)

Microbial Collections – FINEP/CRB-FIOCRUZ (2008)

– Coleção de Culturas do Gênero Bacillus e Gêneros Correlatos do

Microbial Collections – FINEP/CRB-FIOCRUZ (2008)

IOC

– Coleção de Culturas de Bactérias de Interesse em Saúde do IOC

– Coleção de Culturas de Fungos do IOC

– Coleção Micológica de Trichocomaceae do IOC

– Coleção de Bactérias da Amazônia do ILMD

– Coleção de Fungos da Amazônia do ILMD

– Coleção de Bactérias da Mata Atlântica do IOC

– Coleção de Trypanosoma de Reservatórios Silvestres, Domésticos e Vetores do IOC

e Vetores do IOC

– Coleção de Fungos Patogênicos do IPEC

– Coleção de Microrganismos de Referência do INCQS

(11)

Fiocruz Biological Fiocruz Biological Collections

(12)

Collection webpage s

visibility

on-line access to the

on-line access to the service: strain

identification, deposit

http://clioc.ioc.fiocruz.br

identification, deposit and distribution

Strain catalogue http://clioc.ioc.fiocruz.br

(13)

Science & technology in health Science & technology in health

– Biological and biomedical research: basic laboratory

research; traditional disciplines (such as parasitology, microbiology, immunology, genetics, etc.) and new microbiology, immunology, genetics, etc.) and new disciplines (such as genomics and pos-genomics), re-organized in complex scenario

organized in complex scenario

– Public health research: health systems and services; epidemiology and surveillance

epidemiology and surveillance

– Clinical research: studies on individual and group population; clinical assays

population; clinical assays

– Technological research: innovation in medicines, vaccines and diagnostic

(14)

Problems in C&T in health (1)

– National fragility on health innovation

– High dependency of knowledge generated on other

– High dependency of knowledge generated on other countries

– Industry vulnerability of the health industrial complex

– Disarticulation among industrial, technological and

– Disarticulation among industrial, technological and health policies

(15)

Problems in C&T in health

– Dissociation between research and burden of diseases

Problems in C&T in health

– Dissociation between research and burden of diseases (particularly concerning neglected diseases)

– Individual health prevails over collective health;

– Knowledge produced is scarcely utilized for policies and health program definition

program definition

– Deficient structure for biotechnology

– Weakness of the system for scientific production assessment

Fiocruz must contribute to overcome this technological and economical gap with R&D and production

(16)

Worldmapper

: area of countries proportional to

Worldmapper

physicians working

(17)

Worldmapper

HIV/AIDS prevalence

(18)

Worldmapper

malaria cases

(19)

The Sisyphus challenge in Chile The Sisyphus challenge in Chile

• “Developing countries share disbelief

about the benefits of the endogenous

production of science as a tool for

economical growth. Hence, public policies

to strengthen science and technology and

promote the culture of innovation are, in

general, weak and sometimes incoherent”

Scientometrics 72(1):93-103, 2007

(20)

On-going initiatives in the country On-going initiatives in the country

National Conferences on Science, Technology and

National Conferences on Science, Technology and Health Innovation (2000, 2004, 2010)

National Agenda of Priorities on Health Research

Private-Public Partnership (PPP) and Innovation Laws

Innovation policy: pharmaceutic (BNDES)

Creation of ABDI and INPI re-organization

Competitiveness forum of Biotechnology

Expansion of offer and quality of pos-graduation programs on health

(21)

Fiocruz Response Fiocruz Response

Production of:

– Vaccines of the National Immunization Program, in collaboration with Instituto Program, in collaboration with Instituto Butantan (SP)

– Diagnostic kits for the main infectious disease

– Anti-retrovirals and drugs for controlling and treating the main endemies (malaria, TB, schistosomiasis, leishmaniasis, etc)

schistosomiasis, leishmaniasis, etc)

– Bio-drugs: α−interferon, erythropoietin

– Medicines of continual use (hypertension,

– Medicines of continual use (hypertension, diabetes)

Health-BRC

(22)

CRB-Saúde (Health-BRC) CRB-Saúde (Health-BRC)

VISION VISION

To build a collection – unique in the world – of biological To build a collection – unique in the world – of biological material related mainly to neglected diseases of Latin America in order to support scientific research, epidemiological studies, as well as the development and epidemiological studies, as well as the development and production of bioproducts directed to diagnosis vaccine and drugs.

(23)

MISSION

To collect, preserve, indentify and catalogue, following international standards of Quality, a representative collection of microrganisms which are relevant to epidemiology surveillance, particularly those related to neglected diseases, and to the technological development of products with significance to human Health.

(24)

CRB-Saúde (Health-BRC) CRB-Saúde (Health-BRC) (Primary) scope Virus Virus Bacterial cultures Fungi cultures Protozoa cultures Protozoa cultures

(25)

Staff

Specialists in the following fields: microbiology, parasitology, genetics, cellular biology, molecular biology, and/or tropical medicine medicine Topics of interest Taxonomy Evolution Molecular phylogeny Microbial diversity Molecular epidemiology

(26)

Working Plan for the BRC Network: Health-BRC Working Plan for the BRC Network: Health-BRC

Meta Etapa

1. Elaboração do Projeto CRB-Saúde 1.1. Definição de parcerias nacionais e estrangeiras

1.2. Participação em reuniões estratégicas

1.3. Chamada institucional para definir a composição do acervo

acervo

1.4. Visita e avaliação das coleções proponentes 1.5. Estruturação do projeto

2. Construção do prédio do CRB- 2.1. Licitação e contratação de empresa para elaboração dos

2. Construção do prédio do CRB-Saúde

2.1. Licitação e contratação de empresa para elaboração dos projetos básico e executivo

2.2. Elaboração do projeto básico 2.3. Elaboração do projeto executivo 2.3. Elaboração do projeto executivo 2.4. Licitação para a construção do prédio 2.5. Construção do Prédio

3. Aparelhamento do CRB-Saúde 3.1. Elaboração do Termo de Referência para licitação para

aquisição dos equipamentos

3.2. Licitações para aquisição dos equipamentos 3.3. Aquisição e recebimento dos equipamentos 3.3. Aquisição e recebimento dos equipamentos 3.4. Instalação dos equipamentos

(27)

Proposal of a model for biological material management

Health Centers , Outpatients units, Hospitals, universities

Proposal of a model for biological material management

BIOLOGICAL MATERIAL Hospitals, universities A N A L Y T IC A L MATERIAL RECEPTION CENTER COLLECTIONS RESEARCH & TDI

P R E -A N A L Y T IC A L Lab COLLECTIONS RESEARCH & TDI

A N A L Y T IC A L A N A L Y T IC A L Lab BRC BRC Lab _Lab Lab Lab Lab A N A L Y T IC A L Epidemiological surveillance Epidemiological surveillance SVS SVS P O S T -A N A L Y T IC A L FINAL RESULT surveillance surveillance SVS SVS P O S T A N A L Y T IC A L FINAL RESULT QUALITY CONTROL