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Bioactive potential of Rosa micrantha Borrer ex Sm. and Castanea sativa Mill. flowers as functional food ingredients: antioxidant and antifungal activity

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aiOLOGII

-·y-L.R. Bioressources, Biolosie

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BIOLIUAL

L' Association Tunisienne

Pour la

Ualorisation des Bioressources

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JOURNÉES SCIENTIFIQUES INTERNATIONALES

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Ses Journées Scientifiques lnternationales sur la Valorisation des Bioressources 02- 04 mai 2014, Hôtel FRAMISSIMA Rcgcncy****, Monastil·, Tl/NISIE

Liste des conférenciers aux Cinquiemes Journées

CONFÉRENCE I: Pr Mohamcd HAMMAMI

Lab. Nutrition- Aliments Fonctionnels et Santé Vasculaire, Faculté de Médecine, Monastir, TUNISIE

Apport de la valorisation des co-et sous-produits dans !e secteur oléicole

CONFÉRENCE II Pr. Isabel FERREIRA

Laborato1y of Applied Chemistry and Biochemistry (LQBA), School ofAgriculture ofthe Polytechnic lnstitute ofBragança, PORTUGAL.

Bioactivity of mushrooms: antioxidant, antitumor and antimicrobial properties.

CONFÉRENCE III : Professc111·. GRIZEAlJ Dominiquc

LUNAM Université, CNRS, GEPEA, Université de Nantes, UMR 6144, Saint-Nazaire, France Caractérisation de cultures continues de cyanobactéries en mode diazotrophique

CONFÉRENCE fV: Profcsseur David MAZl/RAIS

IFREMER, Lab. Adaptation Reproduction et Nutrition des poissons, Brest FRANCE. Sensibilité et acclimatation des larves de poissons marins à leur environnement

CONFÉRENCE V: Professeur Nicolas RABET UMR 7208 BOREA MNHN, Paris FRANCE

Les grands branchiopodes du Maghreb: biodiversité et ressources biologiques

CONFÉRENCE VI: Profcsscur Laura GASCO

Department of Agricultura!, Fores! and Food Sciences, University ofTorino, !TAL Y

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:;cs Journécs Scicntifiqucs Intcrnationales sur la Valorisation dcs Biorcssourccs 02-04 mai 2014, Hôtcl FRAMISSIMA Rcgency*"*", Monastir, TUNISIE

SESSION III Modérateurs 1-11130 I 51115 151-115 151-130 151-130 15H45 151-145 161100 161-100161-115 16111516HJO 161130 171-130

BIOTECHNOLOGIE VEGETALE & ENVIRONNEMENT

Pr Zohra MARZOUK & P1·. Isabel FERREIRA CONF[RENCE II Pr. Isabel FERREIRA Laboratory of Applied

Chemistry and Lliochemistry (LQBA)_ School of Agriculture ofthe Polytechnic

lnsutute o!' r3ragança_ PORTUGAL.

"Bioactivity of mushrooms: antioxidant, antitumor and antimicrobial properties".

C.O.II Bioactive potential o r Rosa micrantlw Borre r ex Sm. and ( 'astanea saliva

Mill. flowers as functional food ingredients: antioxidant and antifungal activity

BARROS Lillian & FERREIRA Isabel C.F.R.

C.O.I2 Valorisation desplantes aromatiques et médicinales dans I'activité

anticorrosion. Cas: lnhibition de conosion du cuivre dans une Solution d'acide nilrique par I'Huile Essentielle d'Artemisiu

HOUBAIRI Sara, ESSAHLI Mohamed et LAMIRI Abdeslam

C.O.I3 Phytochemical constituents and antioxidant activity of stems and leaves of Purslane (J>ortulaca oleraceu L.) growing in Tunísia

DABBOU FEKIH Sam ia, DABBOU Sihem, GUIDO Flamini,

CHAHDOURA Hassiba et H ELA L Ahmed Noureddine

C.O.I4 Valorisation desplantes aromatiques et médicinales dans l'agriculture biologique: étude de l'activité insecticide d'une huile essentielle sur les ravageurs des céréales

EL-MIZIANI lnaam, LHALOUI Saadia et LAMIRI Abdeslam C.O.IS Élude de l'effet de la résistance génétique à la cécidomyie: Mayetivlu

destructur Say., sur le compot1ement agronomique d'une population de blé

dur au Maroc

OURINICI-IE Siham, NSARELLAH Naserlhaq, LHALOUI Saadia el HILALI Abderraouf

I'Al'SE CM~: et

Ses,iun e-I'OSTER (II) d11 l'ostcr N° 37 au l'oster N° 72

Laboratoire de recherche Bioressources : Biologie lntégrative & Valorisation "BIOLIV AL"-Institui Supérieur de

Biotechnologie de Monastir-.\~·l):_,y_j]i;l_l_>y_nU:_ç,_,_,, TéL (+216) 73 465 405; Fax. (+216) 73 465 404 Page 3

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Bioactive potential of Rosa micrantha Borrer ex Sm. and Castanea sativa Mill. flowers as functional food ingredients: antioxidant and antifungal activity

Lillian Barros*, Isabel C.F.R. Ferreira

Mountain Research Center (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1172, 5301-855 Bragança, Portugal

*[email protected]

Natural products represent a rich source of biologically active compounds and are an example of molecular diversity, with recognized potential in drug discovery and development. Particularly, the plant kingdom offers a wide range of natural antioxidants, recognized by having remarkable medicinal properties [1,2]. These beneficial effects could be related to the high content in phenolic compounds, known for being strong scavengers of free radicals, which have key roles in ageing and various diseases, such as coronary heart disease, cancer or neurodegenerative diseases [3]. Phenolic compounds of Castanea sativa Mill. and Rosa micrantha Borrer ex Sm. flowers from Northeastern Portugal were characterized by our research group using HPLC-DAD-ESI/MS. Furthermore, it was performed a screening of their antifungal potential against Candida species (C. albicans, C. glabrata, C. parapsilosis and C.

tropicalis) and the antioxidant activity was accessed by four in vitro assays: scavenging

effects on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals, reducing power (ferricyanide Prussian blue assay), inhibition of β-carotene bleaching and inhibition of lipid peroxidation in brain cell homogenates by TBARS (thiobarbituric acid reactive substances) assay. The detailed results and all the authors that contributed to the studies are available in references [1-3].

Hydrolysable tannins (e.g. tri and digalloyl HHDP glucose) were the main group of phenolic compounds in C. sativa, while flavonoids (e.g. (+)-catechin and procyanidin dimers and trimers) were the most abundant group in R. micrantha. Thus, the stronger effect showed by this latter against all the Candida species and, particularly its fungicide effects in C. glabrata, might be related to the mentioned flavonoids that were inexistence in C. sativa sample. Otherwise, C. sativa showed the highest antioxidant

activity (EC50 values between 30-110 µg/mL of extract).

Therefore, these flowers may be incorporated as functional ingredients in food matrices to promote health benefits, but also to increase the products shelf life, through the observed antioxidant and antifungal properties.

Acknowledgements: PRODER project nº 46576- PlantLact for financial support; FCT (Portugal) for financial support to the research centre CIMO (PEst-OE/AGR/UI0690/2011) and L. Barros contract “Programa Compromisso com Ciência-2008”; Ana Maria Carvalho for flowers identification; Celestino Santos-Buelga for supervision of phenolic compounds identification and Mariana Henriques for coordination of antifungal assays.

References:

[1] Guimarães, R., Barros, L., Carvalho, A.M., Ferreira. I.C.F.R. J. Agric. Food Chem., 2010, 58, 6277-6284.

[2]Barros, L., Oliveira, S., Carvalho, A.M., Ferreira. I.C.F.R. Ind. Crops Prod., 2010, 32, 572-579. [3] Barros, L., Alves, C.T., Dueñas, M., Silva, S., Oliveira, R., Carvalho, A.M., Henriques, M., Santos-Buelga, C., Ferreira, I.C.F.R. Ind Crops Prod., 2013, 44, 104-110.

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Cinquiemes Journées Scientifiques sur la Valorisation des Bioressources

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Avenue Tahar Hadded, B.P. 74, 5000, Monastir, TUNISIE. Tél.: +(216) 73 463 711, Fax: +(216) 73 465 404

Referências

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