Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; – Novosibirsk: ICG SB RAS, 2019. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
The second step of the restructuring of the Federal Research Center Institute of Cytology and Genetics was completed in May 2017. Study of the lines of common wheat of breeding of National Center of Grain named after P.P. Assessment of the genetic diversity of barley landraces maintained in the Vavilov Institute for Plant Genetic Resources (VIR) on a global scale.
174 Systems Biology study on the WOX5 role in the distal part of the root meristem. Study of the genetic diversity of maize samples with dark colored kernels from the gene pool of Azerbaijan.
Screening sugar beet samples for the presence of bolting gene
Genetic resources in creating sustainable diseases of introgressive spring wheat forms
Wheat genetic resources as a raw material for healthy food
Triticale-wheat hybrid lines with the vaviloid type of spike branching
Wheat–alien introgression breeding
Analysis of out of the reference transcripts from RNA-seq libraries in crops
Genome composition and divergence
Anatomo-morphological stem features of spring bread wheat varieties
Efficient eradication of potato viruses by induction
Antioxidant enzyme activities of plants
Short-stemmed hybrid lines derived from crosses of triticale with the synthetic wheat
Haploid biotechnology in the selection of Triticum aestivum L
Genetic resources of water caltrop Trapa L
Breeding value of partial waxy wheat samples in Tatarstan
Sources of high protein and gluten content in grain in some wheat species
Development of sweet pepper F 1 hybrids based
Evolution of the S-genome in Triticum and Aegilops
Zinc finger A20/AN1 stress-associated genes, HvSAP, are differentially expressed under drought, salinity
Identification of the stem rust resistance genes in the introgression lines of spring bread wheat
Genomic analysis in soybean breeding
Plant VLP production system based on bacteriophage MS2 coat protein
The influence of heavy metalions on proline accumulation and resistance of plants to saline stress
Expression of sheep pox viral A27L and L1R proteins in prokaryotic and eukaryotic systems
Breeding of spring bread wheat for resistance to fungal pathogens in Western Siberia
Intraspecific variability and mechanisms of pea (Pisum sativum L.) tolerance to toxic metals
The study of state transitions in phyA and phyB mutants of Arabidopsis thaliana
Pollen parent transfer mitochondria to offspring
Creation of early maturing productive forms of cereals using the cell biotechnology and physiologically active compounds
Alterations of differential gene expression during the morphogenesis induction in wheat tissue culture (transcriptome analysis)
Mutagenic effect of phosphemide for induction of mutations of Hordeum vulgare L. and Linum usitatissimum L
The maintenance and exploitation of plant genetic resources – state of the art
Study of the leaf rust resistance gene Lr52 by targeted sequencing
Drought resistance in some Prunus persica (L.) Batsch cultivars damaged with Plum Pox Virus
Microbial RNases are potential therapeutic agents, so the assessment of plant protection against viruses by endophytic bacteria is becoming an important practical problem. In addition, treatment of potato plants with Bacillus bacteria significantly reduced virus infection of potato plants. Disease prevalence in potato plants was significantly reduced from 75% in the control to 18% (B. subtillis 26D) and 33% (B. thuringiensis) in the inoculated plants.
Similarly, the index of virus infection development decreased from 5–10 % in the control to 1–5 % in the inoculated plants. The further study of molecular mechanisms related to bacterial induction of plant defense responses in response to virus infections will lead to a better understanding of stress resistance problems. The endophytic microorganisms studied in this report can become the basis for the creation of biological agents for plant protection.
Phenotypic effects of the Rht-17 dwarfing gene in spring wheat under two climatic conditions
Cultivated sunflower high-throughput genotyping and lipidomic profiling
The effect of various dominant VRN alleles and their combinations on the duration of development phases and productivity
Study of the lines of common wheat of breeding
Use of a synthetic form Avrodes for modification of the genome of common wheat
The collection of stone fruit cultures of the SBI SO SRI “Zhigulevskiye sady” –
Clusters of transcription factor binding sites in plant genomes
Preventive role of Tomato bushy stunt virus RNA-interference suppressor protein in plant immune response
Quantitative real-time PCR as a supplementary tool for molecular cytogenetics
Developmental pathways regulating wheat inflorescence architecture
The study of the localization, structure and expression
Evaluation of leek (Allium porrum) genomic polymorphism using the AFLP method
New antimicrobial gene promoters from chickweed (Stellaria media) for biotechnology of cultivated plants
Biotechnological approaches in breeding and genetic research of soybean
Genetic effects of alien chromosome substitution or translocation in common wheat
Decreasing wild potato toxicity by targeted modification of glycoalkaloid metabolism genes
Modern biotechnological approaches for improvement of nutritional value of grain sorghum
The nature of bilateral symmetry
Identification of DNA markers associated with starch granules morphology of Solanum tuberosum L
Molecular, сytogenetic, and morphological features of primary octoploid triticale
Bioresource collections of vegetable plants as an initial material for breeding cultivars with high biochemical value
Influence of molybdenum and tungsten on the enzymatic activity of molybdenum enzymes
Tissue-dependent transcription of the rye centromeric histone CENH3 variants
Male sterility in potato – perspectives for developing hybrid seed breeding
The shape and structure of the wheat ear is one of the most important characteristics of the grain, related to their economically valuable properties, such as productivity, absence of ear fragility and ease of threshing. The study of the genes that control these traits will allow us to purposefully create new varieties with improved traits in terms of yield, ease of threshing and resistance to environmental factors [1]. The evaluation of wheat ear characteristics in most modern studies is carried out by an expert based on visual analysis and measurement practices, which requires a considerable investment of time, despite the fact that tens of thousands of plants are analyzed in modern experiments.
Automating this time-consuming process through the introduction of digital image analysis technologies is relevant to modern science. We propose the method of wheat tip morphometry based on the analysis of digital images. This method makes it possible to extract a number of characters of the tip, such as tip length, width, area projected on the image, color, marquee volume, etc.
The proposed approach makes it possible to analyze the shape of the ear, which is an important characteristic closely related to the species of the plant, which in turn can be used to identify cultivars. The obtained morphometric data were loaded into the SpikeDroidDB database ([2], http://spikedroid.biores.cytogen.ru). The method showed high accuracy in determining the qualitative and quantitative characteristics of wheat ears.
Identification and characterization of a barley gene controlling cuticle wax formation
Study of genetic basis of the melanin biosynthesis in barley grain
Study of the role of Arabidopsis thaliana RNA-polymerase with dual-targeting RPOTmp in plant early development
Marker-based development of wheat near-isogenic
Assessment of the genetic diversity of barley landraces maintained in the Vavilov Institute of Plant Genetic Resources (VIR)
Association mapping of agronomically important traits in Russian collection of rapeseed
Determination of loci associated with potato starch resistivity to hydrolysis by α-amylase
Direct shoot organogenesis in vitro from mature embryos of maize
Influence of viral suppressor expression on the activity of molybdoenzymes
Study of Sorghum bicolor L. for bioethanol production in the conditions of the South-East of Kazakhstan
WildPetotaDB – a database for genotype and phenotype of wild tuber-bearing species of the genus Solanum.
WildPetotaDB – a database for genotype and phenotype of wild tuber-bearing species of the genus Solanum
Creation and characterization of the soft wheat line with centric translocation T2R.2D
Redesign of starch biosynthetic pathway in rice
Reduced ethylene production in tomato fruits
Study of the introduction collection of the Miscanthus
Expression in potato plants of phosphomimetically mutated gene AteIF2α, coding for alpha subunit of translation initiation
MIGREW database: typical use cases
Assessment of genetic diversity among Siberian stem rust isolates using SSR markers
Genetic diversity of genes involved in fatty acid biosynthesis in a collection of flax cultivars
Expression analysis of CaRab-GTP intracellular vesicle trafficking superfamily genes in response to drought CaRab-GTP superfamily genes in response to drought.
DNA barcodes from four loci provides poor resolution on phylogenetic relationships between the Triticum species
Identification of grain and flour quality determinants in common wheat using GWAS
High-throughput genotyping and transcriptome analysis reveals candidate genes associated with wheat heading time
Evaluation of genetic diversity of Fagopyrum esculentum Moench variety using method of ISSR-analysis
Molecular screening of wheat entries for resistance to tan spot toxins Ptr ToxA and Ptr ToxB Pyrenophora tritici-repentis
Cereal signs analysis associated with color on digital images
Metabolic factors of resistance of bread wheat Triticum aestivum L
Differences in the genetic mechanism of the response to stress between wheat varieties
Identification of the molecular markers linked to the chosen genes in cereals
A transcriptome-base analisys of lilac apical complexes in vivo and in vitro
Postgenomic technologies in practical forestry
Perspectives of using Illumina MiSeq for identifying obligate symbionts of plants – arbuscular mycorrhiza fungi
Marker-assisted selection of new barley genotypes accumulating anthocyanins in grain
The role of expansin and xyloglucan endotransglycosylase genes in the regulation of plant growth under changing environmental
Effect of combined temperature-drought stresses on antioxidant activity of plants
WOX and KNOX transcription factors in symbiotic nodule development
Knockout of abscisic acid (ABA)-dependent transcription factor gene OsVP1 using CRISPR/Cas9 system improves germination
Fine mapping of rice bacterial leaf blight resistance loci to major Korean races of Xoo (Xanthomonas oryzae)
Polyphenolics compound variation in foxtail millet (Setaria italica) germplasm and establish a core collection
Genome-wide association study of powdery mildew resistance in collection of common wheat varieties (T. aestivum L.) in collection of common wheat varieties (T. aestivum L.). One of the most efficient and environmentally important ways of wheat protection against Bgt is the cultivation of the varieties with genetic resistance. The aim of this work was to study the genetic diversity of collection of common wheat varieties and breeding line on resistance to Bgt.
The wheat panel consists of 100 Russian wheat cultivars and 60 breeding lines containing introgressions from species of the tribe Triticeae. The results of the assessment of the level of resistance of wheat cultivars showed that no more than 10% of the varieties have a low level of sensitivity to the population of Bgt, specific for the West Siberian region. Association mapping, performed on the basis of SNP genotyping and phytopathological assessment during three environmental seasons, identified ten loci on chromosomes 1AL, 1DS, 2AL, 2BL, 5AS, 5DS, 6AL, 6DL, 7AL and 7BL.
A major impact on the phenotypic manifestation of the trait was observed for genetic factors located in chromosomes 5AS, 6AL and 6DL. Two loci were mapped in the long arm of chromosome 6D that provide effective protection against powdery mildew. Based on comparative analysis of the chromosomal loci of the known Pm resistance genes and loci mapped in this work, an assumption was made that the QTLs on chromosomes 1DS, 2BL, 5AS and 6DL are novel, previously undescribed resistance loci.
Acknowledgments: GWAS of wheat cultivars was supported by the Russian Science Foundation (project No P); Association mapping of QTLs in introgression lines was supported by the Russian Foundation for Basic Research (project No.
Application of biotechnological approaches in genetic and pre-breeding studies of bread wheat
Polyphenol oxidase gene family in barley (Hordeum vulgare L.)
Genomics of non-photosynthetic plants
Features of the effect of winter wheat selection on grain quality under the conditions of the southeastern region.
Features of the effect of winter wheat selection on grain quality in the conditions of the South-Eastern region
Study of the genetic diversity of oat varieties cultivated in the Tyumen region, by means of aven coding loci.
The study of the genetic diversity of oat varieties cultivated in the Tyumen region, by avenin-coding loci
Molecular-genetic analysis of DNA plasmotype
Genetic resources of Durum wheat in Russia on the content of yellow pigment in grain
Occurrence and variability of polyembryonic seedlings in triticale-wheat hybrid line
Testing safety of genetically modified products of rice
Case study on Sprague Dawley rats
Genetic diversity of rare iris species in the Southern Urals
DNA methylation as a sensitive biomarker of environmental abiotic factor exposure
Characteristics of the interaction of the effector genes ToxA and ToxB with the susceptibility genes Tsn1 and Tsc2.
Features of the interaction of the effector genes ToxA and ToxB with the susceptibility genes Tsn1 and Tsc2
Modern biotechnologies for the targeted modification of wheat genome
Increasing the protein and gluten content in the grain of bread wheat using marker-assisted selection
The gene expression level of enzymatic and non-enzymatic antioxidant system of potato plants under chloride salinity
Duplication of the dominant Vrn-A1b.2 allele in Triticum dicoccum lineage
Fieldwork was carried out at the Absheron Research Station of the Institute of Genetic Resources of ANAS. Pollen mother cells (PMCs) for meiotic chromosome behavior studies were prepared by the standard Carnoy and pumpkin acetocarmine fixation method and observed 35 chromosomes in F1 plants, as expected. Significant differences in chromosome conjugation of reciprocal hybrids were not observed, so they had approximately the same amount of bi- and univalents.
Genetic diversity of hexaploid wheat accessions conserved ex situ in the Japanese NBRP-Wheat gene bank.
Genetic diversity of hexaploid wheat accessions conserved ex situ at the Japanese gene bank NBRP-Wheat
Ecological strain testing of breeding lines of soft spring wheat in Bagan created on the basis of distant hybridization
Phylogenetic analysis of high-throughput sequencing data
Functional characterization
Reactivation of VaSTS1 expression
Association mapping for physio-biochemical traits under salt stress in wheat RILs population developed
Polymorphism of flax pathogens assessed using deep sequencing
Flax (Linum usitatissimum L.) response
Molecular mechanisms of the drought tolerance in common wheat – a transcriptomic approach
Nanocomposite selenium – containing substances and effect on ring rot of potatoes
Sequencing and iterative assembly of Ixiolirion tataricum plastome from total DNA using 2nd and 3rd generation HTS platforms
Databases and computer resources on plant miRNA to study its role in abiotic stress response
The virulence of isolates of Ustilago tritici (Pers.) Jens
Molecular-cytogenetic analysis of common wheat lines with T. kiharae genetic material
To live in genetic diversity: wild emmer in Fertile Crescent and its use for plant breeding
Gene pool of common beans in Western Siberia
Analysis of the evolution of gene expression patterns in flowering plants
Bioinformatics analysis of the structures of CRISPR/Cas-systems in the genomes of phytopathogenic bacteria
Polymorphism of CAD and CESA genes in flax (Linum usitatissimum L.)
Mapping of loci associated with drought tolerance in chromosomes 2A and 2D of bread wheat
The role of E-box, G-box and RY motif-binding proteins in the regulation of the ethylene response in Arabidopsis thaliana and in the regulation of the ethylene response in Arabidopsis thaliana. Ethylene signaling proceeds through a linear pathway that activates EIN3 and EIL1 transcription factors (TF) – the key regulators of the ethylene response. In addition to EIN3 binding to gene promoters, cofactors are often required to initiate gene expression after ethylene treatment.
In this work, we perform a genome-wide analysis of EIN3 binding to investigate complex mechanisms of EIN3-mediated gene expression upon ethylene treatment. Using the Tomtom tool, E-box, G-box and RY-like motifs were annotated as binding sites for bZIP family group I TFs, PIF family TFs and FUS3 binding sites, respectively. Enrichment of the motifs annotated as the binding sites of EIN3-unrelated TFs indirectly supports a possible role for these TFs as EIN3 co-factors.
To further investigate this possibility, we performed an analysis of EIN3 and bZIP29/PIF4/FUS3 binding co-occurrence in gene promoters and found it statistically significant (p <2.2e-16). It is noteworthy that FUS3, PIF family and bZIP family group I TFs are known to participate in various ethylene-regulated processes. Therefore, according to our results, we suggest that FUS3, PIF family and bZIP family group I TFs may influence EIN3 functioning to regulate gene expression at the genome-wide level.
Sex-associated genome region of poplar
Assembling of the Siberian larch mitochondrial genome using long nucleotide sequence reads, the largest currently known mitogenome
Introducing CGMS genes to the commercial and hopeful cotton cultivars of Iran
Development of new methods for obtaining hybrid forms
Intragenomic polymorphism of internal transcribed spacer ITS1 in the locus 35S rRNA of polyploid Avena species
The association mapping of quantitative resistance loci to net blotch and spot blotch in barley
The introgression peculiarities of the wheatgrass 6Ai chromosome in various varieties of common wheat
Patterns of durum wheat response to favorable environments
Major approaches in improving wheat resistance to the crucially dangerous diseases in Kazakhstan
The resistance of different wheat species to greenbug aphid Schizaphis graminum Rond
What we know about vernalization process in wheat
Towards genome-based and environment-informed breeding intensification
Identification and numeration of the univalent chromosomes for cotton monosomic lines by means the tester translocations
Identification and numbering of the univalent chromosomes for monosomic cotton lines by tester translocations. Systems biology study on the role of WOX5 in the distal part of the root meristem in Arabidopsis thaliana.
Systems biology study on the WOX5 role in the distal part of the root meristem in Arabidopsis thaliana
Genome-wide association and epistatic scan
Assessment genetic structure of Azerbaijan wild and cultivated barley genotypes by biochemical marker
The role of Eutrema salsugineum cold shock domain protein EsCSDP3 in the cold-acclimation
Effect of root exudates and rhizobacteria on colonization of barley roots by phytopathogenic fungi Fusarium culmorum
Application of Amplifluor-like SNP markers in plant genotyping
Structural peculiarities and polymorphism
Phenotypic and genotypic evaluation of bread wheat line with introgression from T. timopheevii into 2B chromosome
Genome-Wide Association Mapping of diverse set of spring wheat germplasm in Western Siberia
Radial plant growth – Cellular coordination during growth in two dimensions
Assessment of genetic diversity in the wheat genetic resources based on agricultural traits
Unified automated information system for the formation of highly structured plant genomes and proteomes
Transcriptomic changes underlying partial albinism in barley nearly isogenic line
Anthocyanin pigmentation in wheat and barley
The protective functions of progesterone system of hormonal regulation in higher plants
Analysis of chromosome structure in Musaceae using oligo painting
Development of the substitution lines of bread wheat with introgressed pubescence from T. timopheevii
Plant genetic resources in India: management and utilization
Barley alloplasmic lines – the spectra of peculiar plasmon types
Systems analysis of chilling stress induced transcriptomes in Arabidopsis thaliana
Polymorphism of the stem rust population on avirulence genes in Western Siberia
Application of genetic resources and markers in breeding of potato resistant to late blight
Biochemical, molecular and genetic aspects of fruit ripening in green-fruited and red-fruited tomato species
Stress-inducible and tissue-specific promoters in transgenic tomatoes
