Indirect Immunofluorescence assay
Performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples
... of indirect immunofluorescence assay (IFA), immunochromatography assay (ICA-BD) and reverse transcription-polymerase chain reaction (RT-PCR) for detecting human respiratory syncytial virus ...
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EFFICIENCY OF INDIRECT IMMUNOFLUORESCENCE ASSAY AS A CONFIRMATORY TEST FOR THE DIAGNOSIS OF HUMAN RETROVIRUS INFECTION (HIV-1 and HTLV-III) IN DIFFERENT AT RISK POPULATIONS
... the indirect immunofluorescence assay (IFA) with Western blot (Wb) as a confirmatory method to detect antibodies anti retrovirus (HIV-1 and ...
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Roseolovirus genus within the Betaherpesvirinae sub-
... by indirect immunofluorescence assay (IFA) revealed two main groups: one composed of 57 patients with recent primary HHV-6 infection and another group of 68 patients showing signs of past HHV-6 ...
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Antibodies to Leishmania spp. in domestic felines
... 2010). Indirect immunofluorescence assay (IFA), direct agglutination test (DAT), and enzyme-linked immunosorbent assay (ELISA) are the main serological diagnostic tests, and others include ...
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Comparative serology techniques for the diagnosis of Trypanosoma cruzi infection in a rural population from the state of Querétaro, Mexico
... Immunological diagnostic methods for Trypanosoma cruzi depend specifically on the presence of antibodies and parasitological methods lack sensitivity during the chronic and “indeterminate” stages of the disease. This ...
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Recombinant Yellow Fever Viruses Elicit CD8(+) T Cell Responses and Protective Immunity against Trypanosoma cruzi
... virus. Indirect immunofluorescence assay of Vero cells infected with YF 17DD vaccine virus (A and D), recombinant YF17D/ENS1/Tc viral (B and E), and recombinant YF17D/NS2B3/Tc virus (C and ...
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Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy
... The aim of this study was to evaluate an enzyme-linked immunoassay with recombinant rhoptry protein 2 (ELISA- rROP2) for its ability to detect Toxoplasma gondii ROP2-specific IgG in samples from pregnant women. The study ...
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ADENOVIRUS RESPIRATORY INFECTION: SIGNIFICANT INCREASE IN DIAGNOSIS USING PCR COMPARING WITH ANTIGEN DETECTION AND CULTURE METHODS
... Clinical specimens: We conducted a prospective, descriptive and cross-sectional study. Nasopharyngeal aspirates (NPA) collected from children and immunosuppressed patients with acute respiratory infections hospitalized ...
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Serological identification of Rickettsia spp from the spotted fever group in capybaras in the region of Campinas - SP - Brazil
... using indirect immunofluorescence assay in Pedreira city, São Paulo, located also at Campinas region, and found positive serology in 12 out of 33 dogs ...
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Detection of Cryptosporidium parvum oocysts in calf fecal samples by direct immunofluorescence assay
... The conjugate was used at a dilution of 1:80, in PBS (pH 7.4), with good levels of fluorescence of oocysts, such that the fluorescence only diminished at the dilution of 1:100. Stibbs and Ongerth (1986) standardized the ...
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Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil
... assay (ELISA) and the indirect immunofluorescence assay (IFA), in dogs from localities close to the border region of Foz do Iguaçu city, Paraná, in Argentina and Paraguay, 2012 and 2013[r] ...
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A case of human monocytic ehrlichiosis in Serbia
... subside. Indirect immunofluorescence assay was used to analyze three serum samples obtained from this patient for Ehrlichia chaffeensis antibodies, and peripheral blood smear was evaluated for the ...
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Int. Arch. . vol.17 número3
... Using indirect immunofluorescence, and according to the autoantibodies present, BSLE can be classified into 3 subtypes: type 1, which reacts against collagen VII; type 2, where the location of the antigen ...
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Cad. Saúde Pública vol.24 número2
... The present study discusses the identification of a dog with characteristic symptoms and diag- nosis of canine visceral leishmaniasis by indirect immunofluorescence (IIF) and polymerase chain reaction (PCR) ...
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Zymodeme and Serodeme Characterization of Leishmania Isolates Obtained from Costa Rican Patients
... Indirect immunofluorescence with monoclonal antibodies - The monoclonal antibodies, and their respective reactivity, used in this study are shown in Table ...
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Cad. Saúde Pública vol.22 número3
... American tegumentary leishmaniasis (ATL) was studied in 143 dogs in a rural area in the county of Mariluz, northwestern Paraná State, Brazil, using direct parasite search, indirect immuno- fluorescence (IIF), and ...
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Cad. Saúde Pública vol.33 número12
... the indirect immunofluorescence antibody test (IFAT), the IT LEISH rapid test, the parasitological examination of bone marrow aspirate, the direct agglutination test (DAT-LPC) standard- ized in the Clinical ...
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Partial Protection of Mice against Trypanosoma cruzi after Immunizing with the TcY 72 Antigenic Preparation
... by indirect immunofluorescence; (2) significant DTH after injection of epimastigotes in mice footpads; (3) peak parasitemia in immunized mice was significantly reduced and animals were negative by 13 days ...
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AN IMMUNOFLUORESCENCE TEST FOR DIAGNOSIS OF OPHTHALMIC HERPES IN A MOUSE CORNEAL MODEL
... of efficient anti-herpes therapy has increased the demand for quick and sensitive diagnostic methods. In the present study, we have used our murine model to establish an alternative method for detection of HSV-1 by ...
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Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients
... Antiviral therapy can provide signifi cant clinical benefi t and be life-saving, provided that therapy is initiated promptly. It is therefore es- sential to have an assay that allows the rapid and reliable ...
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