[PDF] Top 20 Dengue virus type 3 in Brazil: a phylogenetic perspective

... detected in the Americas dur- ing dengue fever/dengue hemorrhagic fever (DF/DHF) outbreaks in Nicaragua and Panama, in 1994 (Anony- mous ...this virus has spread to many ... See full document

... of Dengue virus type 2 (DENV-2) was introduced into the Americas in the ...into Brazil, it was first detected in Brazil in ...the virus spread throughout the ... See full document

... and 3 have been causing yearly outbreaks in Brazil. In this study, we report the re- introduction of DENV2 in the coast of Sa˜o Paulo ...with dengue fever during the 2010 ... See full document

... obtained in this study raise some intrigu- ing questions: how can a recent clinical viral isolate from South America display ...patient in China? Will it be possible to trace the route by which these ... See full document

... serotype 3 was identified by indirect immunofluorescence assays performed with type-specific monoclonal ...new dengue serotype in a susceptible population is a real threat for the occurrence ... See full document

... In Brazil, DEN-3 virus was isolated in São Paulo in 1998 from an imported case and since then no more cases were detected in that region, showing no DEN-3 ... See full document

... antigen in C6/36 cells was achieved by the indirect immunofluorescence technique, using a type-specific monoclonal antibody for DENV serotype 3 (3H5) ... See full document

... 1994). Phylogenetic studies focusing on the envelope pro- tein proposed the further classification of DENV-3 into four genotypes (genotypes I, II, III, and ...identified in the Caribbean and Puerto ... See full document

... 2, 3 and 4 (Heinz et ...called dengue haemorrhagic fever/ dengue shock syndrome ...prevalent in over 100 tropical and sub-tropical countries, threatening the lives of 2,5 thousand million ... See full document

... an in-house real-time PCR ...performed in duplicate, with an input of 7.5 ␮l of an RNA template in a final reaction volume of 10 ...for 3 s (annealing), and 72°C for 10 s (extension), run on ... See full document

... time in a singleplex format, with a type-specific primer (DENV-1 to DENV-4) was performed for ...sequenced in both directions by using the BigDye Terminator Cycle Sequence Kit (Applied Biosystems, ... See full document

... found in tropical regions around the ...increase in migratory flow between countries have led to large epidemics and severe clinical out- comes, such as dengue haemorrhagic fever and dengue ... See full document

... outcome in a primary infection case where no individual risk factors were reported suggesting that the virulence of the strain might be responsible for the out- ...using phylogenetic analysis of ... See full document

... 10 in Venezuela serves as a good ex- ample of correlation on the serotypes circulating in the human population and the ones detected in mosquito ...DENV-4 in mosquitoes when four serotypes ... See full document

... Dengue virus (DENV) is an arbovirus transmitted to humans through the bite of infected Aedes aegypti ...mosquitoes. Dengue disease is endemic in several countries of Africa, the Amer- icas, ... See full document

... Global dengue virus spread in tropical and sub-tropical regions has become a major international public health ...role in the immunopathology of the disease and that the identification of ... See full document

... outbreak in Taiwan in 2000, and more recently by Wenming et ...with dengue 2 and 3 viruses in a Chinese patient returning from Sri ... See full document

... isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the ...variant. ... See full document

... Our phylogenetic analysis further shows that virus genomes recovered from the 2019 cases all belong to genotype III (also known as American/Asian ...from Brazil (18 of the 19 sequences col- lected ... See full document

... Amostras do fígado dos animais foram coletadas no 7º dia após a infecção e foram imersos em formol tamponado a 10% em PBS por 24 horas e em seguida, colocados em solução de álcool 70% até o processamento das amostras. ... See full document