[PDF] Top 20 Genet. Mol. Biol. - vol.24 número1-4

... The 149 Arabidopsis thaliana gene products used in the search resulted in 146 matches with E-value ≤ 10 -10 and 55 apparently full-length cDNA clones while the 261 human sequences result[r] ... See full document

... way (A). The enzymes are listed by numbers: 1, dolichol kinase (EC 2.7.1.108); 2, UDP-GlcNAc N-acetylglucosaminephosphotransferase (EC 2.7.8.15); 3, GDP-GlcNAc:GlcNAc beta-1,4-N-acetylglucosaminyltransferase (EC ... See full document

... Chromosome individuality had already been widely discussed. Edmund Beecher Wilson (1856-1939), an American cytological expert working at Columbia Uni- versity supported that idea, and pointed out several favor- able ... See full document

... The cells were cultured as monolayers on 24 x 36- mm glass coverslips attached to a small drop of siliconized grease (Merck) to the bottom of 90-mm diameter Petri dishes. Three coverslips were placed in each Petri ... See full document

... The Brazilian population is known to be genetically diverse, because of which we investigated the allele frequency of the CCR5-m303 mutation in three different Brazilian ethnic groups co[r] ... See full document

... Adrenoleucodistrofia (X-ALD) é uma desordem peroxis- somal com padrão de herança ligada ao X, fenotipicamente heterogênea, caracterizada por uma progressiva desmielinização da substância branca do sistema nervoso central ... See full document

... We report on a 14-year-old girl who presented a multiple congenital anomaly pattern: ablepharon, hypertelorism, telecanthus, macrostomia, helix agenesis of both ears, redundant thick skin and severe hirsutism, the 5th ... See full document

... When cells from the explants present adequate growth (growth halos start to approach one another), fi- broblasts are detached from the bottom of the flask with a 0.25% trypsin-EDTA solut[r] ... See full document

... In the present study we report the first Brazilian CS family in which diagnosis was confirmed by the demonstration of decreased RNA synthesis in cultured fibroblasts exposed to UV-C radi[r] ... See full document

... Testes were squashed in a drop of absolute ethanol- acetic acid, 3:1 (v/v). After removal of the coverslip, the preparations were immersed in 70% ethanol for 5 min and air-dried. One testis of the pair was stained by the ... See full document

... Cloning of EcoRI representative fragments To clone rDNA fragments, we digested genomic DNA and λ EMBL-4 DNA using EcoRI endonuclease. Digested DNAs were mixed in different ratios and ligated using T4 DNA ligase ... See full document

... Blood samples of 100 Brangus Ibagé animals (70 fe- males and 30 males) were collected from the jugular vein using ACD as anticoagulant. DNA was extracted from to- tal blood according to Plante et al. (1992). Four STRs ... See full document

... 0.33, 0.5, 0.67 or 1.0 µ M). Amplification reactions were optimized for 125 maize MapPairs  primers (Research Genetics, USA), that amplify microsatellite loci from all maize chromosomes. Primer sequences were obtained ... See full document

... F 2 seeds were sown in a greenhouse in 2.5-liter pots containing substrate. Ten days after sowing, when the pri- mary leaves had reached approximately two-thirds of their full development, the plants were inoculated with ... See full document

... for 24 h and further fixed in Carnoy (3:1, v/v) for 1-24 ...of 4% (v/ v) cellulase and 40% (v/v) pectinase, and squashed in a drop of 45% acetic ... See full document

... The number of flowers treated was small (Table III), but the hypothesis that this species is an in- breeder that needs mechanical stimulation cannot be ruled out.. Apparently A.[r] ... See full document

... An aliquot of blood (0.3 ml) was added to 5 ml of standard supplemented RPMI 1640 medium containing 20% fetal calf serum and phytohemagglutinin (PHA, 0.2%). The flasks were cultured at 37 ° C. After 44 h, 4 µ g of ... See full document

... In the present study, the drugs tested were cyclophos- phamide and vinblastine sulfate. Cyclophosphamide, an alkylating agent used in chemotherapy, requires metabolic activation before it can act as a mutagenic agent to ... See full document

... Vitavax, the active principle of which is carboxin (2,3 dihydro-5-carboxanilido-6-methyl-1,4-oxathiin), is a fungicide that is particularly toxic to basidiomycetes (Edginton and Barron, 1967; Snel et al., 1970). Azevedo ... See full document

... Senhora da Conceição (Porto Alegre, RS, Brazil). The Eth- ics Committee approved this project. All samples were ob- tained after informed consent according to institutional guidelines. Within 24 h of collection, ... See full document