Mota, Fátima A. R., LAQV, REQUIMTE, Escola Superior de Saúde do Instituto Politécnico da Guarda, Portugal
Pereira, Sarah A. P., LAQV, REQUIMTE, Faculdade de Farmácia da Universidade do Porto, Portugal Çay, Ipek, LAQV, REQUIMTE, Faculty of Pharmacy of Anadolu University, Turkey
Passos, Marieta L. C., LAQV, REQUIMTE, Faculdade de Farmácia da Universidade do Porto, Portugal Araujo, André RTS, LAQV, REQUIMTE, Escola Superior de Saúde do Instituto Politécnico da Guarda, Portugal
Saraiva, M. Lúcia M. F. S. , LAQV, REQUIMTE, Faculdade de Farmácia da Universidade do Porto, Portugal
Lactate is generally present in human blood at very low concentrations and its production is traditionally an anaerobic event [1]. However, lactate is considered a signalling molecule promoting specific immune inflammatory responses since its levels increases according to the grade of inflammation. In physiological conditions, lactate concentrations are between 1.5-3 mM in blood and healthy tissues. However, in inflammatory pathologies, lactate levels rise to 10 mM and in cancer, tissues can increase until 30-40 mM [2].
To determine the lactate concentrations in human samples it was developed a sequential injection analysis system. The reactions take place in a confined space and the computer control assure the precise reaction conditions, in the solutions mixture and in the detection process [3]. The reaction for determine lactate concentrations is based on the conversion of lactate in pyruvate by lactate dehydrogenase. This enzymatic reaction occurs in the presence of nicotinamide adenine dinucleotide that is converted in nicotinamide-adenine dinucleotide reduced which upon excitation at 390 nm emits a fluorescence signal at 470 nm.
The effects of several parameters on the reactions were tested, such as reagents concentration, volumes, and aspiration order, pH, reaction time and selected the best ones to get accuracy and quickness in the execution of the enzymatic assay.
References
[1] Phypers, B.; Pierce J.M.T. Continuing Education in Anaesthesia, Critical Care & Pain, 2006, Volume 6 (3).
[2] Pucino, V.; Bombardieri, M.; Pitzalis, C.; Mauro, C. Eur. J. Immunol. 2017. 47: 14-21 [3] Hansen, E. H.; J. Environ. Sci. Heal. 2005, 40, 1507-1514.
Acknowledgments
Financial support from the European Union (FEDER funds POCI/01/0145/FEDER/007265) and National Funds (FCT/MEC, under Partnership PT2020UID/QUI/50006/2013, FEDER Funds through the COMPETE and by FCT - within the scope of the project POCI-01-0145-FEDER-030163. M.P. thanks FCT for the financia support.
15130 | Strong Magnetoelectric Effect and Spin-Phonon Coupling in Fe-substituted
TbMnO3
Maia, André, Faculdade de Ciências da Universidade do Porto, Portugal Vilarinho, Rui, Faculdade de Ciências da Universidade do Porto, Portugal
Moreira, J. Agostinho, Faculdade de Ciências da Universidade do Porto, Portugal Almeida, Abílio, Faculdade de Ciências da Universidade do Porto, Portugal
Magnetoelectric multiferroics, such as orthorhombic rare-earth manganites, where both magnetic and ferroelectric orders are coupled, have attracted a great interest as they are crucial to magnetoelectric devices processing. In the case of TbMnO3, an incommensurate sinusoidal collinear order of the Mn spins occurs at TN = 41K, wherein the Mn spins lie in the bc-plane (Pbnm setting). Below Tlock=28K, a magnetic transition occurs into a commensurate cycloidal spin order with Mn spins rotating in bc-plane, compatible with the stabilization of an improper ferroelectric polarization along the c-axis [1, 2]. Furthermore, it is possible to magnetically control the polarization, as a magnetic field along the b-axis rotates the cycloidal spin order to the ab-plane, and thus the electric polarization to the a-axis [1].
One way to tune the magnetoelectric coupling is by chemical substitution in TbMnO3. The work done in ceramics shows that the substitution of Mn3+ by small amounts of Fe3+ profoundly changes the magnetic structure, altering the magnetoelectric coupling [3]. However, these studies were done in ceramics, and thus anisotropic effects cannot be ascertained, such as the flop of the cycloidal plane with an applied magnetic field.
In this work, we used oriented single crystals of TbMn1-xFexO3 with x = 2, 4 and 6% to measure polar and dielectric properties versus temperature and magnetic field along the crystallographic directions, as well as lattice dynamics, magnetic excitations and spin-phonon coupling through Raman spectroscopy. The obtained results will be presented emphasizing the effect of temperature and magnetic field on the physical properties for the different x-values. One major outcome of this study is the invariance of the electric polarization direction with increasing iron concentration.
[1] T Kimura et al, Phys. Rev. B, 71(22), 224425 (2005) [2] M Mochizuki et al, Phys. Rev. B, 80(13), 134416 (2009) [3] R. Vilarinho et al, JMMM, 439, 167 (2017)
15132 | Families in Museums: Proposal for the Casa-Museu Marta Ortigão
Sampaio
Diogo, Andreia S., Faculdade de Letras da Universidade do Porto, Portugal
Museums are places with an increasingly strong educational and social function. At the same time, it is necessary to develop connections with the public through strategies that support learning in their spaces. Although they have a significant influence on the number of visitors in leisure facilities and museums, families are a group for which there aren´t many studies in the national context. Similarly, very few museums have programmes specifically for this audience. The Report aims to present a summary review of the bibliography on this topic and a reflection on the curricular internship at the Casa-Museu Marta Ortigão Sampaio. It´s also proposed a family activity to be applied in the Casa-Museu, and the creation of a fold-out to support family visits. To do so, the methodology contemplated the review of the bibliography on this topic, the data collection about educational programs for families in certain international and national museums (including Museus Municipais do Porto), and a case study that took form as the internship at the Casa-Museu. This permitted to explore issues such as What are families? What characteristics do they have? Why do they visit museums? What kind of programmes are planned to families? What difficulties do they feel during the visit? What are the implications of family characteristics in museum practices? How do Museus Municipais do Porto see families? What do Museus Municipais do Porto offer to families who visit them?
As main results, families are presented as a multigenerational group with a variable composition, allowing to find different family types in contemporary society. The internship was essential to know the space, public, dynamics and potential of the Casa-Museu, showing that families aren´t the large slice among the visiting public, but that the desire for changing the panorama is present. Keywords: families; educational sector; Museus Municipais do Porto; education policies and practices