Levando em consideração que não foi possível obter as células knockout para o gene da TcMPx e que a expressão da Cas9 não era tóxica para as células, a TcMPx parece ser essencial para a sobrevivência do parasita. Entretanto, não é possível se descartar também algum problema na técnica utilizada que impossibilitou a geração do knockout do gene.
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8. Anexos
DECLARAÇÃO
Em observância ao §5º do Artigo 1º da Informação CCPG-UNICAMP/001/15, referente a Bioética e Biossegurança, declaro que o conteúdo de minha Dissertação de Mestrado, intitulada “Avaliação da importância funcional da triparedoxina peroxidase mitocondrial para o
Trypanosoma cruzi”, desenvolvida no Programa de Pós-Graduação em
Biologia Funcional e Molecular do Instituto de Biologia da Unicamp, não versa sobre pesquisa envolvendo seres humanos, animais ou temas afetos a Biossegurança.
Assinatura:
Nome do(a) aluno(a): Giulia De Andrade Marinho
Assinatura:
Nome do(a) orientador(a): Fernanda Ramos Gadelha