CONCLUSÃO GERAL

Mostramos que proteínas das famílias PR-1 e Taumatina-like, inicialmente descritas e encontradas apenas em plantas, também são encontradas no genoma do M. perniciosa, indicando uma evolução e diversificação da função dessas proteínas.

Experimentos de análise de exportação de lipídeos indicam que as proteínas MpPR-1d, MpPR-1k, MpPR-1g, MpPR-1h e MpPR-1j apresentaram a capacidade de complementar o fenótipo da S. cerevisiae de se ligar e exportar colesterol. Por outro lado, as proteínas MpPR-1a, MpPR-1b, MpPR-1c, MpPR-1e, MpPR-1f e MpPR-1i não apresentaram a capacidade de complementar o fenótipo indicado diversificação funcional. Corroborando com isso, tivemos o experimento de ligação in vitro, no qual observamos que a MpPR-1d se ligou ao colesterol enquanto que a MpPR-1i não. Entretanto, a MpPR-1i se ligou ao palmitato assim como observado com outras duas proteínas SCP/TAPS, a SmVAL4 e a Tablilisina-15, nos mostrando mais uma vez a diversificação na função das MpPR-1.

MpPR-1s, inibem a toxicidade de fitoanticipinas como a alfa-tomatina. Isso indica a grande importância dessas proteínas para o sucesso do estabelecimento do fungo.

Acreditamos que, com a doença praticamente estabelecida na planta, o fungo produz três MpTLPs (MpTLP2, MpTLP4 e MpTLP13) no estágio de vassoura seca com o intuito de inibir o desenvolvimento de fungos oportunistas que podem competir com o M. perniciosa por alimento. Acreditamos ainda que a sTLP MpTLP12, expressa na fase de necrose da doença, possa estar antecipando essa competição com os fungos oportunistas antes mesmo da morte do tecido infectado do cacau.

Este trabalho demonstra um interessante caso em que proteínas primeiramente identificadas como relacionadas com a defesa vegetal contra patógenos são utilizadas por um fungo justamente para este sobrepujar o sistema imune inato vegetal.

A partir do exposto, demonstramos que o presente trabalho pode contribuir para o melhor entendimento sobre os mecanismos das funções das MpPR1s e MpTLPs na Vassoura de Bruxa. Além de agregar novas informações aos esforços que vêm sendo empreendidos no Projeto Vassoura de Bruxa. Estas podem se converter em conhecimentos capazes de gerar possíveis alvos para o controle da Vassoura de Bruxa, além de agregar novas informações aos esforços que vêm sendo empreendidos no Projeto Vassoura de Bruxa. Estas informações, especialmente a resolução estrutural das MpPR-1, podem se converter em conhecimentos capazes de gerar possíveis alvos para o tão sonhado controle da Vassoura de Bruxa.

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ANEXO I

The Fungal Pathogen Moniliophthora perniciosa Has Genes Similar to Plant PR-1 That Are Highly Expressed during Its Interaction with Cacao.

Paulo J.P.L. Teixeira, Daniela P.T. Thomazella, Ramon O. Vidal, Paula F.V. do Prado, Osvaldo Reis, Renata M. Baroni, Sulamita F. Franco, Piotr Mieczkowski, Gonçalo A.G. Pereira, Jorge M.C. Mondego.