CONCLUSÃO

Com os resultados obtidos nesse trabalho foram possíveis as seguintes conclusões:  Nanoemulsões tipo O/A foram formadas pelo método de emulsificação por inversão de

fases utilizando NMP como co-solvente.

 NMP adicionada ao sistema SOR 25 permitiu a formação de sistemas newtonianos, viscosos, com tamanho nanométrico, isotrópicos, com gotículas esféricas. Estes sistemas aumentaram a solubilidade e a liberação do BNZ.

 A técnica de obtenção e a razão dos constituintes utilizada permitiu a estabilidade por 60 dias das nanoemulsões tipo O/A.

 Nanoemulsões biocompatíveis e com atividade tripanocida sobre epimastigotas e trimpomastigota foram superiores ao BNZ na sua forma pura.

 A validação e o desenvolvimento de um método analítico para o BNZ a partir do sistema nanoemulsionado, contendo fosfatidilcolina de soja, oleato de sódio, Miglyol e NMP, por cromatografia de alta eficiência demonstrou especificidade, linearidade, precisão, exatidão, robustez e baixo limite de detecção e quantificação. Além de utilizar baixas concentrações de solvente orgânico associadas a um tempo curto de ensaio.

PERSPECTIVAS

A partir dos resultados e conclusões obtidos neste trabalho, tem-se como perspectivas:

 O estudo da adição de outras moléculas com função de direcionamento do fármaco nas características físico-químicas do sistema formado, além de seu efeito sobre a liberação do BNZ e sua atividade tripanocida sobre diferentes formas de vida do parasito;

 A avaliação da atividade anti-tripomastigota in vitro dos sistemas contendo ácido siálico;

 A avaliação da atividade tripanocida in vitro sobre a forma amastigota do parasito dos sistemas nanoemulsionados contendo ou não moléculas de direcionamento, incluindo o ácido siálico ;

 Análise do mecanismo de morte celular envolvida com os sistemas de interesse por diferentes técnicas;

 O estudo da eficácia in vivo dos sistemas nanoemulsionados por via oral e parenteral em camundongos conforme protocolo já aprovado pelo CEUA da UFRN (n° 16/2015).

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