A terapia fotodinâmica mediada pela Curcumina como corante nas concentrações de 15μg/ml e 30 μg/ml e com o Diodo Emissor de Luz (LED) (455nm, 600mW/cm2) nas dosimetrias de 36J/cm2 e 72 J/cm2 promoveu completa inativação das espécies de Candida albicans avaliadas.
Não houve diferença na sensibilidade aos efeitos da TFD entre as espécies considerando as dosimetrias 36J/cm2 e 72 J/cm2.
Ocorreu redução no crescimento da Candida albicans quando do uso da curcumina 30 μg/ml na ausência de LED mostrando significância estatística quando comparado ao grupo controle (solução salina) e aos grupos LED
Não houve inativação de Candida albicans quando foi usado a irradiação com LED na ausência de Curcumina.
48
REFERÊNCIAS
Akpan A, Morgan R. Oral candidiasis. Postgrad Med J. 2002;78:455-9.
Alexiades-Armenakas M. Laser-mediated photodynamic therapy. Clin Dermatol. 2006;24:16-25.
Alves OA. Avaliação da eficiência do diodo emissor de luz (led) emitindo em
460 nm associado à curcumina na fotossensibilização letal de candida albicans e de aggregatibacteractinomycetemcomitans estudo “in vitro” [dissertação].
Belo Horizonte: Universidade Federal de Minas Gerais; 2011.
Andrade MC, et al. Effect of different pre-irradiation times on curcuminmediated photodynamic therapy against planktonic cultures and biofilms of candida spp.
Archives of oral biology. 2013;58:200-10.
Breskey JD, et al. Photodynamic therapy: occupational Hazards and preventative recommendations for clinical administration by healthcare providers.
Photomedicine and Laser Surgery. 2013;31(8):398-407.
Bruzell EM, Morisbak E, Tonnesen HH. Studies on curcumin and curcuminoids. XXIX. Photoinduced cytotoxicity of curcumin in selected aqueous preparations.
Photochem Photobiol Sci. 2005;4:523-30.
Buytaert E, Dewaele M, Agostinis P. Molecular effectors of multiple cell death pathways initiated by photodynamic therapy. Biochim Biophys Acta. 2007;1776: 86-107.
Campos MLG, Barbosa VS, Ruiz KGS, Sallum EA, Sallum AW. Curcumina como promissor agente fotossensibilizador para uso em periodontia. Perionews. 2013; 7(4):367-82.
Conover WJ. Practical nonparametric statistics. 2a. ed. New York: Editora John Wiley & Sons; 1980. 495 p.
Dai T, et al. Concepts and principles of photodynamic therapy as an alternative antinfungal discovery platform. Frontiers in Microbiology. 2012;3:120.
Dovigo LN, Pavarina AC, Mima EGO, Giampaolo ET, Vergani CE, Bagnato VS. Fungicidal effect of photodynamic therapy against fluconazole-resistant candida albicans and candida glabrata. Mycoses. 2011;54:123-30.
Dovigo LN, Pavarina AC, Ribeiro AP, Brunetti IL, Costa CA, Jacomassi DP, et al. Investigation of the photodynamic effects of curcumin against candida albicans.
49
Donnelly RF, Mccarron PA, Tunney MM. Antifungal photodynamic therapy. Microbiol
Res. 2008;163:1-12.
Douglas LJ. Candida biofilms and their role in infection. Trends Microbiol. 2003;11: 30-6.
Eggimann P, et al. Epidemiology of candida species infections in critically ill non-immunocompromised patients. The Lancet Infectious Diseases. 2003;3(11):685– 702.
Eptein J, Samderson IR, Macdonald TT. Curcumin as a therapeutic agent: the evidence from in vitro, animal and human studies. Br j Nutr. 2010;103:1545-57. Garcia-Gomes AS, Curvelo JA, Soares RM. Ferreira-Pereira A. A curcumin acts synergistically with fluconazole to sencitize a clinical isolat of candida albicans showuing a MDR phenotype. Med Mycol. 2012;20:251-60.
Giroldo LM, et al. Photodynamic antimicrobial chemotherapy (PACT) with methylene blue increases membrane permeability in candida albicans. Lasers Med. Science. 2009;24(1):109-12.
Grice EA, Segre JA. The human microbiome: our second genome. Annu Rev
Genomics Hum Genet. 2012;13:151-70.
Haukvik T, Bruzell E, Kristensen S, Tønnesen HH. Photokilling of bacteria by curcumin in different aqueous preparations: studies on curcumin and curcuminoids XXXVI. Pharmazie. 2009;64:666-73.
Hegge AB, Ardersen T, Melvik AG, Sik RH, Dahl TA. Formulation and bacterial phototoxity of curcumin loaded alginate foams for wound treatment applications: studies o curcumin and curcuminoides XLII. J Pharm Sci. 2011;100(1):174-85. Hilf R. Mitochondria are targets of photodynamic therapy. J Bioenerg Biomembr. 2007;39(1):85-9.
Jabra-Rizk MA, Falkler WA, Meiller TF. Fungal biofilms and drug resistance. Emerg
Infect Dis. 2004;10:14-9.
Karkowska-Kuleta J, et al.
Fungi pathogenic to humans: molecular bases of virulence of candida
albicans, cryptococcus neoformans and aspergillus fumigatus. Acta Biochimica
Polonica. 2009 Jun 56(2):211-24.
Kato IT, et al. Antimicrobial photodynamic inactivation inhibits candida albicans virulence factors and reduces in vivo pathogenicity. Antimicrob Agents Chemother. 2013 Jan 57(1):445-451.
konan YN, et al. State of the art in the delivery of photosensitizers for photodynamic therapy. Journal of Photochemistry and Photobiol B: Biology. 2002 Mar
50
Konopka K, Goslinski T. Photodynamic therapy in dentistry. J Dent Res. 2007;86:694-707.
Lambrechts SAG, Aalders MCG, Van Marle J. Mechanistic study of the
photodynamic inactivation of candida albicans by cationic porphyrin. Antimicrob
Agents Chemother. 2005;49:2026-34.
Leitão GLNC. Avaliação do efeito fotodinâmico da curcumina sobre candida
albicans: estudo in vitro [doutorado]. São Paulo: Universidade Cruzeiro do Sul; 2014.
LI DD, XU Y, et al. Fluconazole assists berberine to kill fluconazole-resistant candida albicans. Antimicrob Agents Chemother. 2013 Dez 57(12):6016-27.
Lyon JP, et al. Photodynamic therapy for pathogenic fungi. Mycoses. 2011 Set 54: 265-71.
Maisch T. A new strategy to destroy antibiotic resistant microorganisms: antimicrobial photodynamic treatment. Mini Rev Med Chem. 2009 Jul 9(8):974-83.
Martins CVB, et al. Curcumin as a promising antifungal of clinical interest. J
Antimicrobial Chemotherapy. 2009 Nov 63(2):337-9.
Maver-Biscanin M, et al. Fungicidal effect of diode laser irradiation in patients with denture stomatitis. Lasers Surg. Med. 2004;35:259-62.
Maver-Biscanin M, Stipetic-Mrvak M, Jerolimov V. Effects of low-level laser therapy on candida albicans growth in patients with denture stomatitis. Photomedicine and
Lase Surgery. 2005 Jun 23(3):328-32.
Meisel P, Kocher T. Photodynamic therapy for periodontal diseases: state of the art.
J Photochem Photobiol B. 2005;79(2):159-70.
Mitra S, et al. Effective photosensitization and selectivity in vivo of candida albicans by meso-tetra (N-methyl-4-pyridyl) porphine tetra tosylate. Lasers Surg Med. 2011 Abr 43(4):324-32.
Pereira CA, Romeiro RL, Costa AC, Machado AK, Junqueira JC, Jorge AO.
Susceptibility of candida albicans, staphylococcus aureus, and streptococcus mutans biofilms to photodynamic inactivation: an in vitro study. Lasers Med Sci. 2011;26: 341-8.
Perni S, et al. Nanoparticles: heir potential use in antibacterial photodynamic therapy.
Photochem Photobiol Sci. 2011 Maio 10(5):712-20.
Pfaller MA, Messer SA, Hollis RJ, Boyken L, Kroeger J, et al. Variation in
susceptibilitu of bloodstream isolates of candida glabrata to fluconazole according to patient age and geographic location in the United States in 2001 to 2007. J Clin
51
Priyadarsini KI. Photophysics, protochemistry and photobiology of curcumim: studies from organic solutions, bio-mimetics and living cells. J Photochem Photobiol C. 2009;10:81-95.
Queiroga AS, Trajano VN, Lima EO, Ferreira AFM, Queiroga AS, Limeira Jr FA. In vitro photodynamic inactivation of candida spp. by different doses of low power laser ligh. Photodiagnosis and Photodynamic Therapy. 2011;8:332-6.
Rajasekaran SA.Therapeutic potential of curcumin in gastrointestinal diseases.
World J Gastrointestinal Pathophysiol. 2011;15:1-4.
Ribeiro MS, et al. Terapia fotodinâmica antimicrobiana. In: CIOSP, 23., 2005, São Paulo. Livro Virtual. São Paulo: Netodonto; 2005. [citado 15 mar 2015]. Disponível em: http://www.netodonto.com.br/ciosp/index.php.
Rossoni RD, et al. Comparação da eficácia fotodinâmica do azul de metileno, azul de toluidine e verde de malaquita contra candida albicans In: ENCONTRO LATINO AMERICANO DE PÓS-GRADUAÇÃO, 8., 2008, São José dos Campos. Anais
eletrônicos... São João dos Campos: UNESP, 2008. [citado 13 jun 2015].
Disponível em:
http://www.inicepg.univap.br/cd/INIC_2009/anais/arquivos/0422_0113_01.pdf. Sharma RA, Gescher AJ, Steward WP. Curcumin: the story so far. Eur J Cancer. Oxfort. 2005 Set 41(13):1955-68.
Sousa GR. Análise comparative da emissão de luz por led e lasers emitindo no
vermelho do espectro eletromagnético na redução de bactérias periodontais:
estudo “in vitro” [doutorado]. Belo Horizonte: Universidade Federal de Minas Gerais; 2007.
Wilson M, Mia N. Sensitisation of candida albicans to killing by low-power laser light.
J Oral Pathol Med. 1993 Set 22(8):354-7.
Zenia B, et al. Killing of cutaneous microbial species by photodynamic therapy. The