REFERÊNCIAS ADELBERG, J.W.; RHODES B.B.; SKORUPSKA, H.T.; BRIDGES, W.C. Explant Origin Affects the Frequency of Tetraploid Plants from Tissue Cultures of Melon. Hortscience, v. 29, n.6, p.689-692, 1994. ALVAREZ-PARDO, V.; FERREIRA, A.G. Armazenamento de sementes de orquídeas. Revista Brasileira de Sementes, v. 28, n. 2, p. 92-98, 2006. ARRIGONI-BLANK, M. F.; SANTOS, M. S.; BLANK, A. F.; RABBANI, A. R. C.; SILVA-MANN, R.; SANTOS, J. B.; COSTA, A. S.; MENEZES, T. S. A. Analysis of genetic diversity of Laeliinae (Orchidaceae) in the State of Sergipe using ISSR markers. Genetics and Molecular Research, v. 15, n. 2, p. 1-9, 2016. BHATIA, R.; SINGH, K. P.; SHAMA, T. R.; JHANG, T. Evaluation of the genetic fidelity of in vitro-propagated gérbera (Gerbera jamesonii Bolus) using DNA-based markers. Plant Cell Tissue Organ Culture, v. 104, p. 131–135, 2011. BHATTACHARYYA, P.; KUMARIA, S.; NIKHIL, J.; R, TANDON P. Phyto-molecular profiling and assessment of antioxidant activity within micropropagated plants of Dendrobium thyrsiflorum: a threatened, medicinal orchid. Plant Cell Tiss Organ Culture, v.122, p.535-550, 2015. BUYUN, L.; LAVRENTYEVA, A.; KOVALSKA, L.; IVANNIKOV, R. In vitro germination of seeds of some rare tropical orchids. Acta Universitatis Latviensis, Biology, v. 676, p. 159– 162, 2004. CORDEIRO, G.M.; MORAES, C. P.; MASSARO, R.; CUNHA, C. Desenvolvimento in vitro de Cattleya amethystoglossa Lindley x (Cattleya dupreana x Laelia purpurata Lindley) em diferentes meios de cultura. Revista Científica Eletrônica de Agronomia, v. 18, n. 1, p. 22-28, 2011. CHAPLA, P. I.; BESSON, J. C. F.; OLIVEIRA, L. K.; SILVA, J. M. da; ROCHA, A. C. S.; STEFANELLO, S. pH, carvão ativado e agentes geleificantes do meio de cultura no crescimento in vitro de Miltonia flavescens Lindl. Plant Cell Culture & Micropropagation, v. 5, n. 2, p. 87-93, 2009. CHEN, C.; CHEN, W-H. Somaclonal Variation in Orchids. In.: CHEN, W-H., CHEN, F.-C. (Eds.) Orchid Biotechnology. World Scientific: Taiwan. Pp. 65-76., 2007. CHA-UM, S.; KIRDMANCE, C. Minimal growth in vitro culture for preservation of plant species. Fruit, Vegetable and Cereal Science and Biotecnology, v. 1, n. 1, 13-25, 2007 CRUZ, D. T. D.; BORBA, E. L.; VAN DEN BERG, C. O gênero Cattleya Lindl. (Orchidaceae) no Estado da Bahia, Brasil. Sitientibus: série Ciências Agrárias, 3 (1,2), p. 23-34, 2003. DE, L. C.; PATHAK, P.; RAO, A. N.; RAJEEVAN, P. K. Commercial Orchids. De Gruyter Open Ltd. 2015. DOYLE, J.J.; DOYLE, J.L. A rapid isolation procedure for small quantities of fresh tissue. Phytochemistry Bulletin, v. 19, p. 11-15, 1987. FERREIRA, D. F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, v. 35, n. 6, p. 1039-1042, 2011. GIRI, L.; JUGRAN, A.; RAWAT, S.; DHYANI, P.; ANDOLA, H.; BHATT, I.D.; RAWAL, R.S.; DHAR, U. In vitro propagation, genetic and phytochemical assessment of Habenaria edgeworthii: an important Astavarga plant. Acta Physiologiae Plantarum, v.34, p. 869-875, 2012. GALDINIANO-JUNIOR, R. F. Criopreservação, indução de poliploidia e avaliação da estabilidade genética de orquídeas. 2013. 111f. Tese (Doutorado em Agronomia - Genética e Melhoramento de Plantas) – Universidade do Estado de São Paulo, Jaboticabal. GAO, X.; YANG, D.; CAO, D.; AO, M.; SUI, X.; WANG, Q.; KIMATU, J.N.; WANG, L. In Vitro Micropropagation of Freesia hybrida and the Assessment of Genetic and Epigenetic Stability in Regenerated Plantlets. Journal of Plant Growth Regulation., v.29, p.257-267, 2010. GIRI, L.; JUGRAN, A.; RAWAT, S.; DHYANI, P.; ANDOLA, H.; BHATT, I. D.; RAWAL, R. S.; DHAR, U. In vitro propagation, genetic and phytochemical assessment of Habenaria edgeworthii: na importaant Astavarga plant. Acta Physiologiae Plantarum, v. 34, n. 3, p. 869-875, 2011. GROUT, W.V. Conservation invitro. Acta Horticulturae. n. 289, p.171-178, 1991. HAMMER, Ø.: HARPER, D.A.T.: RYAN, P. D. PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica v. 4, n.1, 9pp., 2001. HINSLEY, A.; BOER, H. J.; FAY, M. F.; GALE, S. W.; GARDINER, L. M.; GUNASEKARA, R. S.; KUMAR, P.; MASTERS, S.; METUSALA, D.; ROBERTS, D. L.; VELDMAN, S.; WONG, S.; PHELPS, J. A review of the trade in orchids and its implications for conservation. Botanical Journal of the Linnean Society, v. 186, n. 4, p. 435-455, 2018. HIROCHIKA, H.; SUGIMOTO, K.; OTSUKI, Y.; TSUGAWA, H. KANDA, M. Retrotransposons of rice invloved in mutations induced by tissue culture. PNAS, v.93, p. 7783-7788, 1996. HOSSOMI, S. T.; CUSTODIO, C. C.; SEATON, P. T.; MAARKS, T. R.; MACHADO-NETO, N. B. Improved assessment of viability and germination of Cattleya (Orchidaceae) seeds following storage. In Vitro Cellular & Developmental Biology – Plant, v. 48, p.127–136, 2012. JACCARD, P. Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles, v. 44, p. 223-270, 1908. JALIGOT, E.; RIVAL, A.; BEULÉ, T.; DUSSERT, S.; VERDEIL, J.L. Somaclonal variation in oil palm (Elais guianeensis Jacq.): the DNA methylation hypothesis. Plant Cell Reports, v.19, p.684-690, 2000. KANWAR, K.; DEVI, V.; SHARMA, S.; SONI, M.; SHARMA, D. Effect of physiological age and growth regulators on micropropagation of Aloe vera followed by genetic stability assessment. National Academy Science Letters, v. 38, n.1, p. 29-35, 2015. KISHOR, R.; DEVI, H.S. Induction of multiple shoots in a monopodial orchid hybrid (Aerides vandarum Reichb.f x Vandastangeana Reichb.f) using thidiazuron and analysis of their genetic stability. Plant Cell Tiss Organ Culture, v. 97, p. 121-129, 2009. KUMAR, N.; REDDY, M. P. In vitro plant propagation: A review. Journal of Forest Science, v. 27, n. 2, p. 61-72, 2011. LARKIN, P. J.; SCOWCROFT, W. R. Somaclonal Variation - a Novel Source of Variability from Cell Cultures for Plant Improvement. Theoretical and Applied Genetics, v.60, p.197-214, 1981. LIU, F.; HUANG, L.L.; LI, Y. L.; REINHOUD, P.; JONGSMA, M. A.; WANG, C. Y. Shoot organogenesis in leaf explants of Hydrangea macrophylla “Hyd1’ and assessing genetic stability of regenerates using ISSR markers. Plant Cell Tissue Organ Culture. v. 104, p. 111– 117, 2011. MIGUEL, C.; MARUM, L. An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond. Journal of Experimental Botany, v.62, n.11, p. 3713-3725, 2011. LOPEZ- PUC, G. An effective in vitro slow growth protocol for conservation of the orchid Epidendrum shlorocorymbos SCHLTR. Tropical and Subtropical agroecosystems, v. 16, p. 61-68, 2013. MORAES, L. M.; FARIA, R. T.; CUQUEL, F. L. Activated charcoal for in vitro propagation of brazilian orchids. Acta Horticulture, v. 683, p. 383-390, 2005. MURASHIGE, T.; SKOOG, F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, v. 15, p. 473-497, 1962. OSENA, G.; AMUGUNE, N.O.; NYABOGA, E.N. Genetic Stability of Cassava Plants Regenerated Through Organogenesis Using Microsatellite Markers. Journal of Plant Sciences, v. 5, n. 1, p.19-28, 2017. PINTO, J. S. R.; FREITAS, R. M. O.; PRAXEDES, S. C. Stimulation of in vitro development of Cattleys granulosa by sucrose. General and Applied Plant Physiology, v. 36, p. 183-188, 2010. PIVETTA, K. F. L.; MARTINS, T. A.; GALDIANO-JUNIOR, R. F.; GIMENES, R.; FARIA, R. T.; TAKANE, R. J. Crescimento in vitro de plântulas de Caularthron bicornutum em diferentes concentrações de sacarose. Ciência Rural, v. 40, n. 9, 2010. PESCHKE, V.M.; PHILLIPS, R.L. Genetic implications of somaclonal variation in plants. Advances in Genetics, v. 30, p. 41-75, 1992. PEAKALL, R.; SMOUSE, P.E. GenAlex 6.5: genetic in Excel. Population genetic software for teaching and research-an update. Bioinformatic, n. 29, v. 19, p.2537-2639, 2012. REDDY, M. P.; SARLA, N.; SIDDIQ, E. A. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, n. 128, p. 9- 17, 2002. RESENDE, S. V. Micropropagação e conservação in vitro de Melocactus glaucescens Buining & Brederoo e Melocactus paucispinus G. Heimen & R. Paul (Cactaceae), espécies endêmicas da Bahia e ameaçadas de extinção. 2010.140 f. Tese (Doutorado de Botânica) – Programa de Pós-Graduação em Botânica, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia. SAMARFARD, S.; KADIR, M. A.; KADZIMIN, S. B.; HALIMI. M. S. Genetic stability of in vitro multiplied Phalaenopsisgigantea protocorm-like bodies as affected by chitosan. Notulae Botanicae Horti Agrobotanici, v.41, n.1, p.177-183, 2013. SAMARFARD, S.; KADIR, M.A.; KADZIMIN, S.B.; SAUD, H.M.; RAVANFAR, S.A. In Vitro Propagation and Detection of Somaclonal Variation in Phalaenopsisgigantea as Affected by Chitosan and Thidiazuron Combinations. Hortscience, v. 49, n.1, p. 82-88, 2014. SCATENA, V. L.; NUNES, A. C. Anatomia de Pleurothallisrupestris Lindl. (Orchidaceae) dos campos rupestres do Brasil. Boletim de Botânica da Universidade de São Paulo, v. 15, p. 35-43, 1996. SCHNEIDERS, D.; PESCADOR, R.; BOOZ, M. R.; SUZUKI, R. M. Germinação, crescimento e desenvolvimento in vitro de orquídeas (Cattleya spp., Orchidaceae). Revista Ceres, v. 59, n. 2, p. 185-191, 2012. SHERIF, N.A.; BENJAMIN, J.H.F.; KUMAR, T.S.; RAO, M.V. Somatic embryogenesis, acclimatization and genetic homogeneity assessment of regenerated plantlets of Anoectochilus elatus Lindl., an endangered terrestrial jewel orchid. Plant Cell Tiss Organ Cult., v. 132, p.303-316, 2018. SREEDHAR, R.V.; VENKATACHALAM. L.; BHAGYALAKSHMI, N. Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanillaplanifolia Andrews) as assessed by molecular markers. Biotechnology Journal, v. 2, n.8, 1007-1013, 2007. SOLANO, M.C.P.; RUÍZ, J.S.; ARNAO, M.T.G.; CASTRO, O.C.; TOVAR, M.E.G.; BELLO, J.J.B. Evaluation of in vitro shoot multiplication and ISSR marker based assessment of somaclonal variants at different subcultures of vanilla (Vanilla planifolia Jacks). Physiology and Molecular Biology of Plants, 7p. Published online 02 February. https://doi.org/10.1007/s12298-019-00645-9 SUZUKI, R. M.; ALMEIDA, V.; PESCADOR, R.; FERREIRA, W. M. Germinação e crescimento in vitro de Cattleya bicolor Lindley (Orchidaceae). Hoehnea, n. 37, v. 4, p. 731-742, 2010. TORRES, A. C.; CALDAS, L. S.; BUSO, J. A. (Eds.) Cultura de tecidos e transformação genética de plantas. Brasília: Embrapa-SPI/Embrapa- CNPH. p. 183-260, 1998. TORRES, A. C.; FERREIRA, A. T.; SÁ, F. G. de; BUSO, J. A.; CALDAS, L. S.; NASCIMENTO, A. S.; BRIGIDO, M. de M.; ROMANO, E. Glossário de Biotecnologia Vegetal. Brasília, DF: Embrapa Hortaliças, 2000. 128 p. VALLEDOR, L.; MEIJÓN, M.; HASBÚN, R.; JESUS CAÑAL, M.; RODRÍGUEZ, R. Involvement of DNA methylation in tree development and micropropagation. Plant Cell Tissue Organ Culture, v.91, p. 75-86, 2007. VIANA, C. M. Propagação e conservação in vitro de Cattleya elongata Barb. Rodr. (Orchidaceae Juss.). 2013. 82 f. Dissertação (Mestrado em Genética e Biodiversidade) - Instituto de Biologia, Universidade Federal da Bahia, Bahia, 2014. VIEIRA, T. L.; BARROS, F; ROQUE, N. Orchidaceae no município de Jacobina, estado da Bahia, Brasil. Hoehnea, v. 41, n.3, p. 469-482, 2014. WOLFE, A. D.; XIANG, Q. Y.; KEPHANT, S. R. Assessing hybridization in natural population of Penstemon (Scropulariaceae) using hypervariable Inter Simple Sequence Repeat (ISSR) band. Molecular Ecology, v. 7, p. 1107-1125, 1998. ZABICKI, P.; SLIWINSKA, E.; MITKA, J.; SUTKOWSKA, A.; TULEJA, M.; MIGDALEK, G.; ZABICKA, J.; SLOMKA, A.; KWIATKOWSKA, M.; KUTA, E. Does somaclonal variation play advantageous role in conservation practice of endangered species? comprehensive genetic studies of in vitro propagated plantlets of Viola stagnina Kit. (Violaceae). Plant Cell, Tissue and Organ Culture, v. 136, n. 2 p. 339-352, 2019. ZHANG, F.; LV, Y.; DONG, H.; GUO S. Analysis of genetic stability through inter simple sequence repeats molecular markers in micropropagated plantlets of Anoectochilus formosanus No documento UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS PROGRAMA DE PÓS-GRADUAÇÃO EM RECURSOS GENÉTICOS VEGETAIS - PPGRGV (páginas 71-79)
