Tendo em conta os objectivos definidos para este estudo pretendemos observar que a marcação de Hsp27 e Hsp70 em testículos normais e criptorquídeos de cães apresenta intensidades diferentes em diferentes tipos de células.
A marcação de Hsp27 é de uma maneira geral mais fraca nos dois grupos de testículos, quando comparado com a marcação da Hsp70.
Para o marcador Hsp27, as células de Sertoli e células mióides apresentaram uma manutenção da intensidade de marcação entre os dois tipos de amostras, enquanto nas células germinativas (SPG) e células de Leydig, esta intensidade decresceu nas amostras de testículos criptorquídeos.
Quanto ao marcador Hsp70, as células de Sertoli apresentaram um aumento na intensidade de marcação nas amostras criptorquídeas, e uma manutenção dos níveis altos de intensidade na marcação das células germinativas (SPG). As células de Leydig também apresentaram manutenção dos níveis de intensidade de marcação, mas desta feita em níveis intermédios. As células mióides decresceram os seus níveis de marcação em amostras criptorquídeas. Será do maior interesse, em futuros estudos, estudar melhor as características das Hsp27, Hsp70 e outras Hsp, incluindo as suas isoformas, no testículo, uma vez que se trata de um local com uma população celular que apresenta características próprias, relativamente ao que se verifica noutras localizações, e que permitirá vislumbrar com maior clarividência a totalidade de papéis funcionais das Hsp’s.
Dever-se-ão aprofundar os resultados obtidos neste trabalho com outros posteriores que abordem estas temáticas, assim como ampliar a amostra de forma a tornar estes estudos mais fidedignos.
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Bibliografia
Acland, M.H.; Carlton, W.W., Mcgavin, M.D. 1998: Patologia veterinária especial de Thompson, 2ª Ed. Artmed, Porto Alegre, pp.575-576
Adly, Mohamed A.; Assaf, Hanan A.; Hussein, Mahmoud Rezk A. 2008: Heat shock protein 27 expression in the human testis showing normal and abnormal
spermatogenesis, Cell Biology International 32, 1247-1255
Amann, R.P.; Veeramachaneni, D.N.R. 2007: Cryptorchidism in common eutherian mammals, Reproduction 133, 541-561
Amann, R.P., Veeramachaneni, D.N.R. 2006: Cryptorchidism and associated problems in animals, Animal Reproduction and Biotechnology 3(2), 108-120
Amann, R.P. 1986: Reproductive physiology and endocrinology of the dog, Current therapy in theriogenology, Vol.2; Saunders Company, pp.536-537
Amann, Rupert P. 2008: The Cycle of the Seminiferous Epithelium in Humans: A Need to Revisit?, Journal of Andrology 29(5), 469-487
Arya, Richa; Mallik, Moushami; Lakhotia, Subhash 2007: Heat shock genes – integrating cell survival and death, Journal Bioscience 32(3), 595-610
Billig, Håkan; Chun, Sang-Young; Eisenhauer, Karen; Hsueh, Aaron J.W. 1996: Gonadal cell apoptosis: hormonal-regulated cell demise, Human Reproduction Update 2(2), 103-117
Brancho, Deborah; Tanaka, Nobuyuki; Jaeschke, Anja; Ventura, Juan-Jose; Kelkar, Nyaya; Tanaka, Yoshinori; Kyuuma, Masanao; Takeshita, Toshikazu; Flavell,
Richard A.; Davis, Roger J. 2003: Mechanism of p38 MAP kinase activation in vivo, Genes & Development 17,1969-1978
Calderwood, Stuart K.; Mambula, Salamatu S.; Gray Jr, Philip J.; Theriault, Jimmy R. 2007: Extracellular heat shock proteins in cell signaling, FEBS Letters 581, 3689- 3694
Chen, Min; Yuan, Jin-Xiang; Shi, Yu-Qiang; Zhang, Xue-Sen; Hu, Zhao-Yuan; Gao, Fei; Liu, Yi-Xun 2008: Effect of 43ºC treatment on expression of heat shock
proteins 105, 70 and 60 in cultured monkey Sertoli cells, Asian Journal of Andrology 10(3), 474-485
36
de Kretser D. M.; Kerr, J,B,; Paulsen, C.A. 1975: The Peritubular Tissue in the Normal and Pathological Human Testis. An Ultrastructural Study, Biology of Reproduction 12, 317-324
Dix, David J. 1997: Stress proteins in reproductive toxicology, Environ Health Prospect 105(4), 436-438
Dix, David J.; Allen, James W.; Collins, Barbara W.; Mori, Chisato; Nakamura, Noriko; Poorman-Allen, Patricia; Goulding, Eugenia H.; Eddy, E.M. 1996: Target gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility, Proceedings of the National Academy of Sciences 93, 3264-3268
Eddy, Edward M. 1999: Role of heat shock protein HSP70-2 in spermatogenesis, Reviews of Reproduction 4, 23-30
Efthimiou, Ioannis; Mamoulakis, Charalampos; Papageorgiou, George; Kazoulis, Sabbas; Prevedorou, Despina; Kontogiorgos, George; Christoulakis, Ioannis 2009: Unilateral Malignant Leydig Cell Tumor of Testis in a Patient With Contralateral Cryptorchidism, Urology Journal 6(1),60-62
Gabai, Vladimir L.; Sherman, Michael Y. 2002: Interplay between molecular chaperones and signaling pathways in survival of heat shock, Journal of Apllied Phisiology 92, 1743-1748
Garrido, Carmen; Mehlen, Patrick; Fromentin, Annie; Hammann, Arlette; Assem, Mahfoud, Arrigo, André-Patrick; Chauffert, Bruno 1996: Inconstant association between 27-kDa heat-shock protein (Hsp27) content and doxorubicin-protecting effect of Hsp27, European Journal of Biochemistry 237, 653-659
Govender D.; Sing Y.; Chetty, R. 2007: Sertoli cell nodules in the undescended testis: a histochemical, immunohistochemical, and ultrastructural study of hyaline deposits, Journal of Clinical Pathology 57, 802-806
Griswold, Michael D. 1995: Interactions between Germ Cells and Sertoli Cells in the Testis, Biology of Reproduction 52, 211-216
Hess, Rex A., Chen, Paul 1992: Computer tracking of germ cells in the cycle of the seminiferous epithelium and prediction of changes in cycle duration in animals commonly used in reproductive biology and toxicology, Journal of Andrology 13(3), 185-190
37
Hess, Rex A.; Cooke; Paul S.; Hofmann, Marie-Claude; Murphy, Kenneth M. 2006: Mechanistic Insights into the Regulation of the Spermatogonial Stem Cell Niche, Cell Cycle 5(11), 1164-1170
Hettinga, Judith V.E.; Lemstra, Willy; Meijer, Coby; Los, Gerrit; de Vries, Elisabeth G.E.; Konings, Antonius W.T.; Kampinga, Harm H. 1996: Heat-shock protein expressionin ciplatin-sensitive and –resistant human tumor cells, International Journal of Cancer 67, 800-807
Hughes, Ieuan A.; Acerini, Carlo L. 2008: Factors controlling testis descent, European Journal of Endocrinology 159, S75-S82
Hutson, John M.; Hasthorpe, Suzanne; Heyns, Chris F. 1997: Anatomical and Functional Aspects of Testicular Descent and Cryptorchidism, Endocrine Reviews 18(2), 259-280
Ivell, Richard; Hartung, Stefan 2003: The molecular basis of criptorchidism, Molecular Human Reproduction 9(4), 185-181
Johnston, J.H. 1965: The undescended testis, Archives Disease in Childhood 40, 113-122
Kersten, W,; Molenaar, G.J.; Emmen, J.M.A.; Schoot, P. van der 1996: Bilateral cryptorchidism in a dog with persistent cranial testis suspensory ligaments and inverted gubernacula: report of a case with implications for understanding normal and aberrant testis descent, Journal of Anatomy 189, 171-176
Kimmins, Sarah; Kotaja, Noora; Davidson, Irwin; Sassone-Corsi, Paolo 2004: Testis-specific transcription mechanisms promoting male germ-cell diffentiation Reproduction 128, 5-12
Klonish, T.; Fowler, P.A.; Hombach-Klonish, H. 2004: Molecular and genetic regulation of testis descent external genitalia development, Development Biology 270, 1-18
Koeva, Y.; Delchev, S.; Georgieva, K; Atanassova, P 2005: Heat Shock protein-70 expression in testis Following Endurance Training of Rats, Proceedings of the Balkan Conference of Biology in Plovdiv (Bulgaria) from 19th till 21st of May 2005, 288-294
Landry, Jacques; Chrétien, Pierre; Lambert, Herman; Hickey, Eileen; Weber, Lee A. 1989: Heat Shock Resistance Conferred by Expression of the Human HSP27 Gene in Rodent Cells, The Journal of Cell Biology 109, 7-15
38
Lanneau, David; Thonel, Aurelie de; Maurel, Sebastien; Didelot, Celine; Garrido, Carmen 2007: Apoptosis Versus Cell Differentiation Role of Heat Shock Proteins HSP90, HSP70 AND HSP27, Prion 1(1), 53-60
Mattos, Marcos; Simões-Mattos, Lucilene; Domingues, Sheila Farhayldes Souza 2000: Cryptorchidism in Dogs, Ciência Animal 10(1), 61-70
Mayer, M.P.; Baku, B. 2005: Hsp70 Chaperones: Cellular functions and molecular mechanism, Cellular and Molecular Life Sciences 62, 670-684
Meistrich, Marvin L. 1986: Critical components of testicular function and sensitivity to disruption, Biology of Reproduction 34, 17-28
Mialot, J.P. 1988: Patologia do aparelho genital masculino; Patologia da reprodução dos carnívoros domésticos, Ed. 1; Metrópole. Porto Alegre, pp.69-71
Mournier, Nicole ; Arrigo, André-Patrick 2002: Actin cytoskeleton and small heat shock proteins: how do they interact?, Cell Stress & Chaperones 7(2), 167-176
Mruk, Dolores D.; Cheng, C. Yan 2004: Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis, Endocrine Reviews 25(5), 747-806
Neuer, A.; Spandofer, S.D.; Giraldo, P.; Dieterle, S.; Rosenwaks, Z.; Witkin, S.S. ,2000: The role of heat shock proteins in reproduction, Human Reproduction Update 6(2), 149-159
Pauli, Daniel; Tonka, Chia-Hwa; Tissieres, Alfred; Arrigo, André-Patrick 1990: Tissue-specific Expression of the Heat Shock Protein HSP27 during Drosophila melanogaster Development, The Journal of Cell Biology 111, 817-828
Payan-Carreira, R.1990: Evolução morfo-funcional do testículo. Determinação da idade á puberdade no macho Maronês, Provas de Aptidão Científica e Capacidade Pedagógica – Trabalho de síntese, UTAD
Pirkkala, Lila; Nykänen, Päivi; Sistonen, Lea 2001: Roles of the heat shock transcription factors in regulation of the heat shock response and beyond, The FASEB Journal 15, 1118-1131
Richards, E.H.; Hickman, J.A.; Masters, J.R.W. 1995: Heat shock protein expression in testis and bladder cancer cell lines exhibiting differential sensitivity to heat, British Journal of Cancer 72, 620-626
39
Richards, Elaine H.; Hickey, Eileen; Webber, Lee; Masters, John R.W. 1996: Effect of Overexpression of the Small Heat Shock Protein HSP27 on the Heat and Drug Sensitivities of Human Testis Tumor Cells, Cancer Research 56, 2446-2451
Ritossa, Ferruccio 1996: Discovery of the heat shock response, Cell Stress & Chaperons 1(2), 97-98
Rogalla, Thorsten; Ehrnsperger, Monika; Preville, Xavier; Kotlyarov, Alexey; Lutsch, Gudrun; Ducasse, Cécile; Paul, Catherine; Wieske, Martin; Arrigo, André- Patrick; Buchner, Johannes; Gaestel, Matthias 1999: Regulation of Hsp27
Oligomerization, Chaperone Function, and Protective Activity against Oxidative Stress/Tumor Necrosis Factor α by Phosphorilation, The journal of Biological Chemistry 274(27), 18947-18956
Romagnoli, S.E., Johnston, Sherley D. - Canine reproduction; The Veterinary Clinics of North America, Small Animal Reproduction, 1991. Vol.21(3) p.533-544
Schett, Georg; Steiner, Carl-Walter; Gröger, Marion; Winkler, Stefan; Graninger, Winfried; Smolen, Josef; Xu, Qingbo; Steiner, Günter 1999: Activation of Fas inhibits heat-induced activation of HSF1 and up-regulation of Hsp70, The FASEB Journal 13, 833-842
Sinsimer, Kristina S.; Gratacós, Frances M.; Knapinska, Anna M.; Lu, Jiebo; Krause, Christopher D.; Wierzbowski, Alexandria V.; Maher, Lauren R.; Scrudato, Shannon; Rivera, Yonaira M.; Gupta, Swati; Turrin, Danielle K.; Cruz, Mary Pauline de la; Pestka, SidneyM Brewer, Gary 2008: Chaperone Hsp27, a Novel Subunit of AUF1 Protein Complexes, Functions in AU-Rich Element-Mediated mRNA Decay, Molecular and Cellular Biology 28(17), 5223-5237
Souza, Fabiana Ferreira de; Leme, Denise Pereira; Uechi, Edilson; Trinca, Luzia Aparecida; Lopes, Maria Denise 2004: Evaluation testicular fine needle aspiration cytology and serum testosterone levels in dogs, Brazilian Journal of Veterinary Research and Animal Science 41(2), 98-105
Syntin, Patrick; Chen, Haolin; Zirkin, Barry R.; Robaire, Bernard 2001: Gene expression in Brown Norway Rat Leydig Cells: Effects of Age and of Age-Related Germ Cell Loss, Endocrinology 142(12), 5277-5285
Tähkä, Klaus M 1989: Local control mechanisms in the testis, International Journal of Developmental Biology 33, 141-148
40
Tavaria, Michael; Gabriele, Tim; Kola, Ismail; Anderson, Robin L. 1996: A
hitchhiker’s guide to the human Hsp70 family, Cell Stress & Chaperones 1(1), 23-28
Thonneau, Patrick F.; Candia, Pegia; Mieusset, Roger 2003: Cryptorchidism: Incidence, Risk Factors, and Potential Role of Environement; an Update, Journal of Andrology 24(2), 155-162
Virtanen, H.E.; Toppari, J. 2008: Epidmiology and pathogenesis of criptorchidism, Human Reproduction Update 14(1), 49-58
Vydra, Natalia; Winiarski, Boleslaw; Rak-Raszewska, Aleksandra; Piglowski, Wojciech; Mazurek, Agnieszka; Scieglinska, Dorota; Widlak, Wieslawa 2009: The expression pattern of the 70-kDa heat shock protein Hspa2 in mouse tissues, Histochemical Cell Biology 132, 319-330
Welsh, Michael J.; Wu, William; Parvinen, Martti; Gilmont, Robert R. 1996: Variation in Expression of hsp27 Messenger Ribonucleic Acid during the Cycle of the Seminiferous Epithelium and Co-Localization of hsp27 and Microfilaments in Sertoli Cells of the Rat, Biology of Reproduction 55, 141-151
Wolgemuth, Debra J.; Laurion, Erika; Lele, Karen M. 2002: Regulation of the Mitotic and Maiotic Cell Cycles in the Male Germ Line, Recent Progress in Hormone Research, The Endocrine Society 57, 75-101
Zakeri, Zahra F.; Welch, William J.; Wolgemuth, Debra J. 1990: Characterization and Inducibility of hsp70 proteins in the Male Mouse Germ Line, The Journal of Cell Biology 111, 1785-1792