4 Material e método
6.3 Informaçoes adicionais
Apesar deste não ser um dos objetivos deste estudo, durante a execução dos experimentos com as soluções MTAD® e Tetraclean®, observamos a tendência destas substâncias alterarem sua
coloração quando armazenadas por períodos de tempo prolongados.
A partir desta observação, foi realizado um teste adicional, onde preparamos as soluções MTAD®, MTAC e Tetraclean®, e armazenamos os tubos em locais expostos à luz, ou protegidos de qualquer fonte de luz. A cada dia foi possível observar o escurecimento gradual das soluções, independente do local onde estavam armazenadas, sendo que o escurecimento foi levemente mais rápido nos tubos expostos à claridade. Após 2 semanas de preparação, as soluções apresentavam coloração em um tom castanho escuro.
Em um segundo momento, observamos que as soluções quando armazenadas em geladeira, a 4oC, não sofriam alteração de cor,
permanecendo límpidas mesmo após 1 mês de preparo. As mesmas soluções foram então armazenadas a 37oC e onde se observou o rápido
escurecimento das soluções. Após 48 horas as soluções já apresentavam um tom castanho escuro semelhante aos das soluções expostas à luz por duas semanas. Imagens das soluções com alteração em sua coloração estão expostas nos Anexos.
Clinicamente, a alteração de cor sofrida pelas soluções poderia determinar a pigmentação da dentina, uma vez que a doxiciclina adere às paredes dentinárias e é eliminada gradualmente7. Também Tay
et al.152 (2007) relataram a ocorrência de uma coloração arroxeada no tecido dentinário de canais radiculares irrigados com NaOCl seguido de MTAD®.
Os resultados obtidos neste estudo reforçam a dúvida quanto à validade do uso de soluções contendo antibióticos em sua formulação. Nossos achados deixaram claro que a ação antibacteriana das soluções Tetraclean® e MTAC decorre principalmente do detergente
cetramida, e não da doxiciclina.
Somado a isto, a alteração cromática sofrida pelas soluções contendo doxiciclina pode determinar uma contra-indicação ao emprego clinico do MTAD® e do Tetraclean®, justificando a continuidade de
pesquisas para o desenvolvimento de soluções irrigadoras com propriedades físico-químicas e biológicas satisfatórias.
7 Conclusão
De acordo com a metodologia empregada neste estudo podemos concluir que:
1. a solução Tetraclean® apresentou melhor ação antibacteriana in
vitro sobre a cepa de E. faecalis VP3-181 e sobre o biofilme que o MTAD®;
2. as variações da fórmula do MTAD®, contendo cetramida em sua composição apresentaram melhor ação antibacteriana in vitro sobre a cepa de E. faecalis VP3-181 e sobre o biofilme que o MTAD®em sua fórmula original;
3. as formulações contendo cetramida e sem conter Tween 80 em sua composição apresentaram melhor ação antibacteriana in vitro sobre a cepa de E. faecalis VP3-181 e sobre o biofilme.
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