Häufiger sind Niederschlagsprobleme während der Blüte und damit Fusarium-Probleme sowie Niederschläge vor der Ernte, die die Qualität (insbesondere die Fallzahlen) stark beeinträchtigen und daher zu geringeren Erträgen führen. Die deutsche Ex-situ-Genbank am Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) in Gatersleben ist eine der größten Sammlungen weltweit. Am Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung in Gatersleben befindet sich eine der größten Ex-situ-Genbanken der Welt.
In addition to the long-term storage and regular regeneration of the material, phenotypic characterization and evaluation data are collected as a prerequisite for gene identification and mapping. Based on that, the post-anthesis drought tolerance index was calculated from the performance of the stressed plants (S) relative to its respective non-stressed controls (C) within the same replicate and calculation. Nowadays, the utilization of the gene bank holdings for crop improvement, including abiotic stress tolerance, is one of the main challenges.
The successful exploitation of genetic resources requires (1) extensive characterization and evaluation and (2) genetic analysis and molecular mapping of the relevant traits.
CSTIS 100
The use of molecular markers facilitates the incorporation of the most important leaf rust resistance genes (Lr genes) responsible for resistance in new varieties and the pyramiding of these genes. Over the past 15 years, many efficient markers for leaf rust resistance genes have been described. The Martonvásár wheat breeding program uses molecular markers linked to leaf rust resistance genes for both of these purposes.
Application of molecular markers in breeding for leaf rust resistance in wheat Application of molecular markers in breeding for leaf rust resistance in wheat 66. In each combination, 10-15 plants were tested for leaf rust resistance in the greenhouse and field. The presence of five leaf rust resistance genes (Lr1, Lr10, Lr26, Lr34 and Lr37) was analyzed in the Martonvásár wheat pool.
Field leaf rust resistance of plants (36 Thatcher-based near-isogenic lines, 4 recurrent parents, donor parents, BC plants, control: Thatcher) was evaluated in an artificially inoculated nursery. Use of molecular markers in breeding for leaf rust resistance in wheat Use of molecular markers in breeding for leaf rust resistance in wheat 68. Use of molecular markers in breeding for leaf rust resistance in wheat Use of molecular markers in breeding for leaf rust resistance in wheat 70.
HELGUERA M, KHAN IA, DUBCOVSKY J, 2000: Development of PCR markers for the wheat leaf rust resistance gene Lr47. DGGE markers linked to leaf rust resistance genes Lr29 and Lr25 in wheat (Triticum aestivum L.). TAR M, PURNHAUSER L, CSÕSZ M, 2008: Identification and localization of molecular markers linked to the Lr52 leaf rust resistance gene in wheat.
Leaf rust resistance may be based on one or more major genes (Lr genes) and/or minor resistance genes that act quantitatively (quantitative trait loci, QTL). Molecular genetic analysis of durable leaf rust resistance of adult plants in the Austrian winter wheat cultivar 'Capo'.
Marker: YES3-3A
Markergestützte Selektion in der praktischen Kartoffelzüchtung – Erfahrungen und Perspektiven Markergestützte Selektion in der praktischen Kartoffelzüchtung – Erfahrungen und Perspektiven 82.
Marker: YES3-3B
PAAL J, HENSELEWSKI H, MUTH J, MEKSEM K, MENÉNDEZ CM, SALAMINI F, BALLVORA A, GEBHARDT C, 2004: Molecular cloning of the Gro1-4 gene conferring resistance to pathotype Ro1 of the root cyst nematode Globodera rostchiensis, based on a candidate gene approach. We want to develop tools for marker-assisted selection in any breeding population that can be bred using one of the two sources, MF-II and TPS67. Late blight, caused by Phytophthora infestans, is one of the most damaging diseases of the potato.
For the success of marker-assisted selection (MAS) in potato breeding programs, important features of the technology involve the relative ease of application, large diag-. Selected, COSII-specific primers targeting the R gene-bearing chromosomal regions were applied to genomic DNA from both parents of the population to amplify fragments. The fluorescence signal was read out on an iQ5 realtime PCR (BioRad) machine and analyzed to detect presence and absence of the target alleles.
When polymorphisms specific to either parent were found, this primer combination marker was used to genotype all offspring of the mapping population and the marker was mapped. The search for a methodology that would combine in one step the detection of the marker and the confirmation that the polymerase chain reaction (PCR) was successful, without the need for further manipulations, was focused on two innovative techniques; high resolution melting (HRM) and KBiosciences Allele-Specifi c PCR (KASPar). Alternative alleles at the 07960 locus indicating blight susceptibility were reliably detected, ensuring the performance of the KASPar assay.
In: Wenzel G, Wulfert I (eds), Conference of the Global Initiative on Late Blight, Hamburg, Germany, p. 339. The response of Bo7212 observed under infection pressure in field trials was different from that of Australian cv. This difference, as well as the divergent origins of the two accessions, suggest that Bo7212 carries an anthracnose resistance gene different from Lanr1 in Tanjil.
Sequenzinformationen aus den Modellgenomen von Medicago truncatula, Lotus japonicus und Pisum sativum werden verwendet, um eng verknüpfte Marker für Anthracnose-Resistenz bei Schmalblättrigen Lupinen zu entwickeln. Vereinfachung der markergestützten Selektion in der Pflanzenzüchtung durch Datenbanken der IT-Breeding GmbH. Implementierung der markergestützten Selektion in der Pflanzenzüchtung.
Malysheva-Otto 1* , Fabian Gerstenberg und Lars-Gernot Otto
Neben der Saatgutkontamination ist auch eine Kontamination über den Boden möglich (KILLERMANN et al. 2008, VOIT und KILLERMANN 2007). Nach der Ernte 2009 stieg das Sporenpotential im Boden für die anfällige Sorte (A) in Baden-Württemberg deutlich an. Die ATPase-Aktivität des gereinigten Proteins wurde durch einen nichtradioaktiven Phosphatfreisetzungstest basierend auf der Malachitgrünbildung gemessen (ROWLANDS et al. 2004).
Darüber hinaus scheinen lebende Pflanzen eine hohe Fähigkeit zu haben, ZON durch die Bildung des maskierten Mykotoxins ZON-Glucosid zu antagonisieren (BERTHILLER et al. 2009b). Mais stellt als C4-Pflanze höhere Ansprüche an die Temperatur während der Keimung und des Auflaufens (VOIT et al. 2009). Allerdings muss berücksichtigt werden, dass insbesondere bei der Quantifizierung von Feinwurzeln noch ungelöste Messprobleme bestehen (PIERRET et al. 2005).
Die meisten dieser Sorten zeigten in deutschen Versuchen auch eine hohe Resistenz gegen Falschen Mehltau (WÄCHTER et al. 2007). Im Vergleich zur anfälligen Sorte waren auch Jubilar, Ataro, Toronto, Tommi, Pegassos und Tarso statistisch signifikant weniger betroffen (WÄCHTER et al. 2007). Dieses Gen scheint jedoch nicht mit den von FOFANA et al. beschriebenen Genen kompatibel zu sein. 2009) wurden als identisch beschrieben.
Die Selektion auf Resistenz kann entweder direkt (phänotypische Selektion) oder indirekt (In-vitro-Selektion, markergestützte Selektion) erfolgen (BÜRSTMAYR et al. 2000). Die Versuche wurden künstlich mit einer Konidiensuspension des Fusarium culmorum-Isolats (B) inokuliert (CHRPOVÁ et al. 2007). Dies gilt insbesondere für den Lr24-Spender, bei dem ein großes Segment transloziert wurde (SCHACHERMAYR et al. 1995).
Darüber hinaus wurden Vollkornströme der Proben einer NIR-Spektrenmessung mit vier verschiedenen NIRS-Instrumenten (Tecator 1241 für Transmissionsmessungen, NIR-Systems 6500 für Reflexions- und Transmissionsmessungen (alle Foss Analytical AB, Höganäs, Schweden), Matrix I für) unterzogen Reflexionsmessungen (Bruker Optics, Ettlingen, Deutschland) mit unterschiedlichen Messprinzipien (NIT, dispersives NIR und FT-NIR) (SCHMIDT et al. 2009). Für die CAPS-Markerentwicklung wurde das Programm SNP2CAPS (THIEL et al. 2004) verwendet, das Restriktionsstellen an SNP-Positionen erkennt. Eine genauere Lokalisierung von Rrs2 war daher mit einer kartenbasierten Klonierungsstrategie nicht möglich (HANEMANN et al. 2009).
Der Ernteschaden (Anzahl Sporen/Korn) wurde durch Saatgutbehandlung und künstliche Saatgutinfektion deutlich beeinflusst (DRESSLER et al. 2009).
TSKornertrag
The survival rates (SR) of the plants were determined as the ratio of living plants after and before winter. To control the expression of the fl oral repressor genes and the silencing construct, we will use the FLP-FRT recombinase system of Saccharomyces cerevisiae (KILBY et al. 1995). Fusarium head blight in barley: identification of the causal Fusarium species in Europe and testing resistance by artificial inoculation.
Vavilov was one of the first to recognize that our plant genetic resources are particularly dangerous and must be preserved for the future (MAXTED et al. 1997). A paired t-test showed significant differences between the genotypes of the species according to the recent germination test. Due to the same harvest year, the same cleaning methods and storage conditions of the genotypes of the investigated species, we can assume that the differences in germination are genetically based.
Based on identical harvest years and conditions such as cleaning and storage conditions, genetic factors for seed longevity can be assumed. About 70% of the registered carrot varieties in the European Union are the result of hybrid breeding. The aim of the present work was to estimate the agronomic and quality characteristics of new wheat breeding lines and to monitor cultivars.
The present work provides the first results of the agronomic and quality performance of the new breeding lines compared to the control cultivars. Analysis of stretch curves was performed as described by GRAUSGRUBER et al. 2002) with the modification that the dough was mixed to optimal consistency by the Promylograph apparatus. By studying star plots of single genotypes, the multivariate performance becomes more pronounced.
Europe and Renaissance are inferior in their performance with the exception of the large volume of bread realized by Europe. A comparative analysis of data on identified candidate genes obtained by the two complementary approaches will be presented.
