Effect of the aplication of fatty acids on "Lipoxygenase Pathway" from soybean leaves.

(1)

K",)+R/I! RF!:;/.!/../

A

rt

ig

o

eL [R(#R

7+,0%%;+%'/070;+%.V+0( .%D0(&%*+%%(;+0D+ &%((#;%&,%( (09%

.A"8"?$A AA%0B?A*?%A;?$A.A%@` ?+_A9R?&aA6?(A,A.6$A%$

"5D2(D4(! >+< !FI$:...+<

E/Z$Z.:_/:Z:Z./

>> > 70-@*0* > >0P0*" ?@*-KP 0 -07T0PA >E PD0 @0+R & #5%C4 C 5&C 45 #5 & (& Q ( & ( ( & # & ?@5%C4 -&QCA 5 5&C 4CR &( !&&'&#5%C4 ?5A# C Q (RQ4C5 Q( L*0:..)C&45%C4 & ,*0:.. )4C#4C5!Q&& #5%C4 & ,R&5 Q (&55##C )5%C4 ( , R& ( #&&' ( #5%C4 C # #&Q4C5 C &Q & 4 # 5%C4 # ## & 5 # & 5 & ! & 4C5 &Q ( R

UCQ L5%C4 _#C _ CR

+&,.0'/0

@5%4 )L %49 %( ! :R:R::R:/,!5 J %( ! =%4 2( <=(%49J ( 1 4% 5 ( ! 5 ( 4!

! :!;5:_R

0 5%4 (5# 12(V ( = # <= 1 5 4(M ! (

5% /_{R0 ! 5%4 5&}

#(<O #<=!(!&5 <O # & 5R

4! 5%4 1 5 5! 5&#(<O 5 L :_{! 9}_{! 4};_!4

<= I_{! 5 #}F₉

5M4 $_R

55 ( 5%4 !5 (5

! = 1 9_R19

(14% 5 !5 2(.\4(5 5 5 R1 <=

O _{!5 !5 1}

95<=( ( :._R

0?+ @5%4 A5 1 ( 4(M :._{R &5M% # 5<=} 5%4 =( !5 ! 5 (5 !5(5% 2( &5M% &5M% R

0 &5M% 5( 5 /&%!2((5 #( #& R -(

5 :/ (! & &V#!5 5

<= =( 5 #::_R

0&5M% 5(1:/%#M2(! 5M ((<=9 r%<O !141B V! 2((5 ( 5 2( 5 = B( :/_{! ( %5 =} 49 5%4 #& 51 ! &5M

:_R

0#(<=5%4 5 ! 5 <= ((Z#(<=!= 1:;_{R0 !} 5 ( 2( ( 55<=?+ @5%4 A# 45! 5 # B 5M4 R

B &#55 5J5<=1 4% ! ( 5%4 ! 5?+ @5%4 A ( ( & BL(!*0:..! 9 5%4 ! 4M5 *0:.. ! # 4!5 ( 95 9 5%4 R

%.,D.+$&,%; $R

>( 5 B)*+"')@R,,

(2)

#5 R0 5 #( 5 5!.U4 !<O 4<=R

%8X

0 5 B 1+/ )5 #&#5%5,! # 5( ! (<O :.1 4% )2(V!9,K

:...!.:\)Z,:._{4 1+ )}

4( #& # 5 %5,R - 5<= (<=2( K:...!.:\)Z,R >( !#!5 4( #& # 5 B 1+

:I_{R 9 # 5 4(#& #}

# .!/;;&5M 85<= (<O 1 4% !4 492(

.fR55%(#;_!

5&R:F_R

X

05%4 1#

4( 50%R:$_R

( /;! (#<= ( (5 4<O B(4 &5M%# R

X8 5

4( 5=#( L1Z# # M )/!./!I,_ 1 Z M )!.!I,_ 1 Z M);!.;!I,_1Z M)I!. I!I,_# #Z# # M)F!.$!.,_ 7)$!I!I, 1MZ M)!.:.!.,!<=I.R 0 (<=/;#.

. 45(5/!I!/I_R

X8

> % %<= 1 5 5%4 /.!/I!.!I!;.!;II._{R( &} 5#V5(R

!

0 <O <O 5 ##

51:_{R ((}

5=?'A*04

&!2( ((( (<=1( (<= (#)**,5;\)5Z,R( #IF/R

0<= 5 % # # <=5 ( ) D_"@04 5 ,_"!@ D05 0 ( R ( # 4 5 545!

5U':R

!X!

0<=&%#54#4

!5?& 5A! 4(( R/._R

( (Q%)/I..%.!I,!41 # 49!#(%I!$@ZR0#<=&% ##5B<=55 5=! ( # %5 15! R

<O # (

5 R/:_!(

/ & )D7, 4 R0 ( # FI! ( %5 #< # <=<=R

.(;,%0(+(((/0

^5X

B 57 M (<= 5%4 # 5 B ( J5<= ( %M4 !( 1 #57 1R0>4(:5 5# (

57) 5#5%4 R 5# 5

1 2(V)_/.L;,!)_:L/,! 5

!519)_:L, 5 ./;&5M

5<=!5 & R

- 5 ( 5 #5%4 57;!IF!.! ( 5 !5 5 2(5 5 9R57;!I 5## &5 4M5 ! 5 9! !57F!. 5## 5 2( 5 9R !2( 5 ( 5%4 5 5# 2( 5 R ( (42(!<= ( %M4 5 &(1R

5 2( 5 9! 5#57F!.5 ( 2( 5 # 57 ;!I! (4 2( ?5A 5%4 (# R

57M5#<=&5M% 1 5 5%4 #& B *0:..4M5*0:.. ! ( J5 <=1 4% #& ! =#%57M #55%4 ( 5 !51 ( ( ( L5%4 (( ! 57 I!IF!.//_{_ 5%4 4 ! 57 F!/}/_{_}

5%4 #& ( 0"!

57 F!;$!//;_{_ 5%4 55! 57 I!.}/I_{_ 5%4} 5=!57F!I/F_{_5%4 &!57F!}/$ 5%4 #& B4M5*0:..*0:.. ! 1+ ! ( # !57F!.F_R

^X - 5( M 5%4 # 5 B ( J5<= 1 4% #(1 !1 5(R0>4(/5 5# ( 5

(3)

:F D K",)

1 2(V)_/.L;,!)_:L/,! 5

!519)_:L, 5 ./;&5M

5<=!5 & R

( 5##/I_!5

4M5 ( 5 R5/I_#

!5=#8 ! 5 < (# 5%4 R+# ( !2( 5## 5 ( J5<=#1 4% 2( 5 ! (4( 5 5 B (5%4 ! # 1 #57R

( = (! 2( ( ( 5( M

/I_{55%4 # BL@}_R/_&

5 4M5 *0:..!>+ ! 1

+; _+RF₅

*0:..4M5*0:.._{! 1+}

!_{( #R}

%!^X

0 <= 5# 5%4 %5 #57F!.5=# # M I./If!5# 575( 2( 2(&( 5 R0 >4( ;5 5#5%4 ! ( 1 ( R

*0:..)>4(,&((4( 5#./;&5M !! 4M5*0:.. )>4(;,&(( 5 #.;&5M ! 5 /"0 )6986'8=")%

) 67 0" 8 8 6 )1 ;CBB%S))"DU6<

"6A5A5CB"%2O_CUISQ"&

W;CBBBBC]O)V)Q6)^6A5A5? & W;CBB BBC] O)V)Q_ 5 "3 2 6AZ ` ) 65

AZ'6"ICBBBm"6588NB"ENaRDm

<CB"RCm'8

K 69 I !" O QI BCB " OQ BTB " O"Q!"O$QIBCB"OQBNB"O"Q

/"$ )6986'8=")% )"60"886 <61 ;CBB&,%S))"DU

6< " 6A5 A5 CB " % 2 O_CUISQ"

&W;CBBBBC]O)V)Q6)^6A5A5 ?&W;CBBBBC]O)V)Q_5"326AZ `)65

AZ'6"ICBBBm"6588NB"67GBRDm

<CB"RCm'8

(4)

/"+ )6986'8=6 O6" 8 8Q O Q 6 2" O 8 8QO$Q6=)1 ;CBB%S ))"BEDDU6<"6A5A5

CB"%?>O_EBIDQ3O_CUIEQ2O_CUISQ

6)^6A5A5?&W;CBB BBC]O)V)Q_5"326AZ`)65

AZ '6"I CBBBm "65 88 NB " 67 GB

ENaRDm<CB"RCm'8

/"1 )6986'8=6 O6" 8 8Q O Q 6 2" O 8 8QO$Q6=)1 ;CBB&,%S ))" B ED DU 6< " 6A5

A5CB"%?>O_EBIDQ3O_CUIEQ2

OCUISQ6)^6A5A5?&

W;CBBBBC]O)V)Q_5"326AZ`)65

AZ '6"I CBBBm "65 88 NB " 67 GB

ENaRDm<CB"RCm'8

#5 4M5*0:.. # 2( 5 *0:..R ( 2(! % #<?5A5%4 ( & ! 5 1 &! !2(<=4 5%4 =#( 5 #& R

- 4M5 ! ( 5#5;&5M 1 ):L/,9):L,! 2( = 55 ( 5%4 4R-! 19):L,#2(5 ( 5# 4M5 ! 5 9! 5 /;;&5M R- # 1 ( #& 2( 1 !4!5 (55 ( %M4R0 ! 1 5 5 5 !5( ( !19!2(1

41B V2(!5 (!5%4 :._R

4M5 ! ( 2(

5# 5 12(V)_/.L;,

# & 5 ! ! #K:..R0 2(!<= ( %M4! 5 & ( 5%4 R-2(5( 5 41 4% 5!K:..# R K:.. ( 4 2(! 2( ! 4 J 4O 5# 5 5 (%

&#M! ( 5 R 5 ((5! 51! #!(#(% ( R(%(45! 4<= ( 5 5% 45R0 !(5 5 (<= ( %M4 2( ( (1(<= ( ! ( 52( ( ( 5%4 !( R

+

?+ @5%4 A55(1B V!

4 2(# 5 :._!(

<=<=5# % # R ( 45 545 5 :/R

> <= 5 4# ; & 5M 8 5<= 1 9! 2( = 55 ( 5%4 4R* ( 5 2( 9! 4M5 R 0 !&( 5 5 !55( <= 5 !55<= ?+ @5%4 AR ( =

5>EC:._2(&5

(5)

: D K",)

,HS*<=5 5 5 5 % #& B!# 4(#&#

5 1+ R+*0:..4M5*0:.. .!/;;&5M 85<= (<=:. 1 2(V)_/.L;,!)_:L/,9)_:L, ( 5 s5<= (<=2( K:...!.:\)Z,tR

AZ'6"I4( J :

5)7

45 Z45

5M 8

E 5 )# 4(#&#,

5<= *0:.. *0:..

(<O 6 6 6 6 6 6

1 4% ! 02(V @ @9 02(V @ @9

( 5%4 ,

. !..:.:_% _!:;;$_% _!..: _!: _I!:F:. _F!F/ _F!F;:I_" _I!:I /; !/I% !:% :.!; :.!;;$ F!.F !F;:^ !$$FI^" :.!/:^ ; $!$I./ !;$;. !F$F :.!;./ /I!$.$F^ /F!$IF$^ !FI :.!./I4 +5:

,FS*<=5 5 5 5 % #& B!# 5#&#

5 1+ R+*0:..4M5*0:.. .!/;;&5M 85<= (<=:. 1 2(V)_/.L;,!)_:L/,9)_:L, ( 5 s5<= (<=2( K:...!.:\)Z,tR

AZ'6"II..@5= 7.!:!57!//._/_:..@%(#_I.@ (<=5 ;!$%:.I_{_I..@ (<="!@D5 0:!/}

5)7

45 Z45

5M 8

E 5 )# 5#&#,

5<= *0:.. *0:..

(<O 6 6 6 6 6 6

1 4% ! 02(V @ @9 02(V @ @9

( 5%4 ,

. I!.;I;: _I!F;$: _I!/;. _$!;:IF _F!$;._% _I!I._% _:.!.:. _::!;I$_" /; !.$: :.!:/ :.!F;$ :!I$I $!:/% I!$;% :;!/I$ :/!$/F." ; ;.!;;$/ !F$ !.:; F!F;$ :!I$:^ .!I/$^ ::;!;/I:^ ::;!$.^

:_{+ = 1 9 5<O R ( ! 4( ( 8 ()(8 (4#,}

=# !I\5!5 ('CR & ! 4( ( 8 ()(8 ( 4#,=# !I\5!5 ('CR

1 2(V!9R0 1 ( # (<= *!%

1 9R ( 5>EC:.

2( 5 5 #& # 1B V 5 !( 4 5 R

05(<= 5 ( ;&5M 19! 5 2( 9 4M5 R (

= 5>EC:._!2(

2( *2((( 5 19#( 2(2( ( 51 5#R

( ! ( 5#!(5 # 5 B J5<=1 4% ?+ @5%4 A! 2( 5 ( #9 5%4 4(! <=&5M% !

&5M%#1:/%#M2(! 5 #1B VR !5 (! 54(5#%

5 =4 2(# 5 :._R

&!X!

0 ?+ @5%4 A 5 5(! ! &%

( /._{R0 !( <=&%}

(6)

&%;&5M 85<= 1 4% ! ( 5%4 R

= &( ( 4# ; & 5M ! 4M5 ! 5 )I,2( 9)F,R

0&;(/0

( (2( 5%4 #& 4M5 !( 1 ( ! % 9 5 ( 5#5%4 57;!I F!.! 5 ! 5 2( 9!5 < (# 5%4 ?5A R 5(M57F!. 5 9#/IfR

( 5# (4( 5 5 B J 5<= 1 4% ! ( 5%4 ! 5 ( ! 2(

<=4 5%4 =# ( 5 5!(2( 4M5 5 5 &R

( 2(! 5 4M5 !5(<= 2(5(<=&%! 5 J5<=1 4% ! ( 5%4 ! #52(R* 2(?+ @5%4 A 1 ! = 4(5(<= 55 5 /_#._{R]5 2(&B<=?+} @5%4 A 5 J5<=1 4% !5M #<=&5M% !5( 51 5%4 ! <=1(&5M% 4( 5(<= 1 B V ( 23 <= %5 =4 2(# 5 R

0 <= 5 2( 5%4 5 #& B!(<= ( #(<O # M4 5#( 4 5(<=42(9B <= ( B 5M4 R

,TS &%5 % #& B!# 4(#&#5 1+ R

+*0:..4M5*0:.. .!/;;&5M 85<= (<=:. 1 2(V)_/.L;,!

)_:L/,9)_:L, ( 5 s5<= (<=2( K:...!.:\)Z,tR AZ'6"I4( J

5)7

E 5 )# 4(#&#,

5M 8 *0:.. *0:..

5<=

(<O 6 6 6 6 6 6

1 4% ! 02(V @ @9 02(V @ @9

( 5%4 ,

. :R;:!.:_% _:/R:!$ _:;R/:!_% _:.R..!$_" _;RFF!. _;R$.:! _IR$F!. _IR$..!$ /; :;RF!. :$R$;F! :;RIF!% :.R$!$" IR://! ::R$!. $R;;!. R.;! ; :R.;!% RFI/! R;F!. :.R.$.!$" $R;F! :RF/!$ R/F!$ :/R:./! +5R0 2(= = 4( =5 <= 4#!I\5!5 &DR

,US &%5 % #& B!# 5#&#5 1+ R

+*0:..4M5*0:.. .!/;;&5M 85<= (<=:. 1 2(V)_/.L;,!

)_:L/,9)_:L, ( 5 s5<= (<=2( K:...!.:\)Z,tR

AZ'6"II.4#& # _:...@5=# #.!I!57$!.!;.fZ/._5( (L;If_5(BL/..f_5(L/I.f_(0EDT0K)/I..%.!I,_41 L

/_#(%41 LI!$@Z

5)7

E 5 )# 5#&#,

5M 8 *0:.. *0:..

5<=

(<O 6 6 6 6 6 6

1 4% ! 02(V @ @9 02(V @ @9

( 5%4 ,

. IRF!.:_% _IRFF.!. _;RF$.!. _IRFF!$ _R/!._" _IR.!. _FRF!. _FR:!$ /; FRF/!$ :$RI$! R.I$!$% :.R:/.!" ;R/;!$" :.R:;!^ R:;$!.^ $R.:!$^ ; IR$F!% $R/I!$ $R//;!% $R;/$!`" $R./:!^ :/RF;/!. $R/! FRI/!.`

:_{+ = 1 9 5<O R ( ! 4( ( 8 ()(8 (4#,}

(7)

/. D K",)

,MS 5 % #& B!# 4(#&#5 1+

R+*0:..4M5*0:.. .!/;;&5M 85<= (<=:. 1 2(V)_/.L;,!)_:L/,9)_:L, ( 5 s5<= (<=2( K:...!.:\)Z,tR AZ'6"I.!I@%#_.!I@ (<=D7.!:\_:!/I@>.!/\_@77

5)7

E 5 )# 4(#&#,

5M 8 *0:.. *0:..

5<=

(<O 6 6 6 6 6 6

1 4% ! 02(V @ @9 02(V @ @9

( 5%4 ,

. .!$/F .!I .!. .!/. .!;I. .! .!;: .!;F.

/; :!.I: _.!F;; _:!./; _:!:I _.!..F _:!.I$/ _.!;;_% _.!/F;

; .!:I .!.F .!; :!.; :!.; :!.I:F .!I/% :!..F;

+5;

,JS 5 % #& B!# 5#&#5 1+

R+*0:..4M5*0:.. .!/;;&5M 85<= (<=:. 1 2(V)_/.L;,!)_:L/,9)_:L, ( 5 s5<= (<=2( K:...!.:\)Z,tR AZ'6"I.!I@%#_.!I@ (<=D7.!:\_:!/I@>.!/\_@77R

5)7

E 5 )# 5#&#,

5M 8 *0:.. *0:..

5<=

(<O 6 6 6 6 6 6

1 4% ! 02(V @ @9 02(V @ @9

( 5%4 ,

. .!;; .!: .!/. .!;.% .!/;% .!: .!;.. .!/:

/; .!;/.: _:!:/$ _:!:: _.!I$_% _.!I_% _:!::I _:!./:_" _.!$._" ; :!.;: :!:. .!F :!.I. :!./$; :!/:;. :!.;/" .!I" +5;

% .%+$&,0(

( 4J>(<=05J- 2( !>0-*!55#R

.7.E&+%(

:R Q!SR R_ )00+0$FGGF?DE!:;IR /R (!UR_7BC!UR_(&!PR_UBQ!R_00+FGGG!CCM!

:/$R

R E(C!R_D((!SR_@(!R_00+FGGH?MU!$:R ;R 5& ! @R R_ D('!RTR_ "( !TR R_ ! 7R "R_0

0+FGGL!CCG!/R

IR -'!RUR_7!R0R_@ 'C!SRR_-!SRR_0 FGGT!

MG!:.R

FR +!0R0R_!RR0R_S !*RR_- ! R"R_E!R R_!R0R_D !RR_)$HIIF!CS!:R $R > #!0R@R_D '!ERR_0+0FGGL!CBE!/I$R R T4!R_U!-RR_#!SRR_7!"R>R_00+FGGG!

ST!;$R

R 7!"R>R_7U5!RER_@44!RR_D'B !R_

&60$"0+FGLL!T!/.:R

:.R >!RR_EC!R0R_0FGGH!D!:/R

::R b!"RR_(!R0R_00FGKG!GS!FFR :/R (!RSR_0+0FGGK!CBBFIR

:R !7R"R_UB!"RR_> &!+RER_00+FGGH!CBB!;R :;R >!R_-( !R_D!R_+!0R_u!>R_0

FGGG!CDN!:R

:IR >&!TR ER_ ! R _ 8 + ()6"!0 L

5% !*Q C!:$$!5R::R :FR &! 7R_ *! R_ '! DR_ !PR_0 0+FGLM!EE!

::R

:$R 0%!DR_& (4&!RR_@ '!R_!+"FGLF!TC!

;;:R

:R &!-RUR_U&!ER*R_7 !RR_!0RUR_!>R 7R_-!R"R_>(B!RUR_'! RR_ !DRSR_U'! "RR_ $"FGLJ!CN!$FR

:R U'!R@R_E' !SRSR_4&!SRR_-( '!R_"FGKT!

NC!$FR

/.R (!RR_D !RR_!RR0R_C!RR_! R0R_)$FGGL!CB!/.R

/:R !*RR_E !TRSR-R_!R0R_E!RR_E&!+RR_ C!RR_)FGGU!DB!;R

//R !R_-&5 !"RER_#$"FGKF!CED!;:R /R @!R_*!ER_-!R_# FGLJ!NB!R

/;R U&! R_ ! DR_ ! 7RR_ ( '!0R_0 0+FGGH!

MM!I$:R

/IR 0( &'!R0R_P!KRR_U(!SR_7!"R>R_0FGGT!CMS! ;R

/FR 4( 2(! R *R_ S! R_ >! SR_

0+"+FGGU!SE!::.R

/$R E4!"R_&Q!R_U(&!7R_$"FGGJ!GB!$:IR

/R @!0RR_!RR0R_D !RR_!R0R_$#

00+FGGM!U!$R

/R !7RTR_$"$6+ FGGF!CBUD!//:R