a teria
Slađa a ). Da ido ić , Mio a G. Miljko ić , Duša G. A to o ić , Mirja a D. Rajilić-Stoja o ić , Suza a I. Di itrije ić-Bra ko ić
U i e sit of Belg ade, Fa ult of Te h olog a d Metallu g , Depa t e t fo Bio he i al E gi ee i g a d Biote h olog , Belg ade, Se ia
U i e sit of Belg ade, Fa ult of Te h olog a d Metallu g , Depa t e t fo O ga i Che ist , Belg ade, Se ia
A st a t
Wate kefi is a e e age fe e ted a i o ial o so tiu aptu ed i kefi g ai s. The kefi g ai s at i is o posed of pol sa ha ide, p i a il de t a , hi h is p o-du ed e e s of the i o ial o so tiu . I this stud , e ha e isolated la ti a id a te ia LAB f o o - o e ial ate kefi g ai s f o Belg ade, “e ia a d s ee ed fo de t a p odu tio . A o g t el e LAB isolates th ee p odu ed sli e ol-o ies ol-o ol-odified MR“ MR“ aga ol-o tai i g su ol-ose i stead ol-of glu ol-ose a d e e p e-su ed to p odu e de t a . Th ee LAB e e ide tified, ased o o phologi al, ph sio-logi al a d io he i al ha a te isti s a d “ RNA se ue i g, as Leu o osto ese -te oides st ai s T a d T a d La to a illus hilga dii st ai T . The isolated st ai s e e a le to s thesize a su sta tial a ou t of de t a i MR“ oth o tai i g % su ose. Ma i al ields . , . a d . g/l e e o tai ed afte , a d h fo T , T a d T , espe ti el . Opti al te pe atu e fo de t a p odu tio as oC fo t o Leu
o-osto ese te oides st ai s a d °C fo La to a illus hilga dii st ai . The p odu ed de t a s e e ide tified ased o pape h o atog aph , hile the ai st u tu e ha a -te isti s of pu ified de t a e e o se ed FT-IR spe t os op . Ou stud sho s that
ate kefi g ai s a e a atu al sou e of pote t de t a p odu i g LAB.
Ke o ds: de t a , ate kefi g ai s, L . ese te oides, L . hilga dii.
“CIENTIFIC PAPER
UDC . . : . .
He . I d. –
doi: . /HEMIND D
A aila le o li e at the Jou al e site: http:// .a he.o g. s/HI/
Wate kefi is a e e age p odu ed fe e tatio of su ose solutio supple e ted ith le o sli e a d d f uits, est figs [ ]. Be e age is slightl a o ated, ith ef eshi g a d ode atel sou taste. The fe e tatio is pe fo ed a o so tiu of s
-ioti i oo ga is s e edded i kefi g ai s. The i o iota of kefi g ai s a ies depe di g o its o igi a d ultu e edia used fo fe e tatio [ ]. The o -positio of the kefi g ai s' i o iota has ee e essi el studied [ – ]. It i ludes easts, a eti a id a -te ia a d la ti a id a -te ia LAB , ith the last ei g the ost a u da t [ ]. LAB of the kefi g ai s elo g to the follo i g ge e a: La to a illus, La to o us a d Leu o osto [ , , ].
The ai o po e t of ate kefi g ai at i is de t a [ ]. This e opol sa ha ide EP“ is s thesized i dige ous LAB. Diffe e t autho s epo ted diffe e t a te ial spe ies as the p edo i a t EP“ p odu e s i ate kefi g ai s a d these i lude La to a illus asei t o su spe ies [ ], Leu o osto ese te oides, La
Co espo de e: “.). Da ido ić, U i e sit of Belg ade, Fa ult of Te h olog a d Metallu g , Depa t e t fo Bio he i al E gi ee i g a d Biote h olog , Ka egije a , Belg ade, “e ia.
E- ail: sda ido i @t f. g.a . s Pape e ei ed: “epte e , Pape a epted: No e e ,
to a illus agelii a d La to a illus ho dei [ ], as ell as La to a illus hilga dii [ , ].
De t a is a high ole ula ass ho opol sa -ha ide ade of glu ose. The glu ose ole ules i the
ai hai of de t a a e li ked alpha - gl osidi o ds. B a hi g a o u at positio s , o [ ]. The p opo tio of a hi g a d the hai le gth affe ts the heologi al p ope ties of EP“ [ ].
Due to its elati e sta ilit a d good solu ilit , de -t a is idel used i diffe e -t a hes of i dus-t ies, su h as edi al, pha a euti al, food, te tile a d
he-i al he-i dust he-ies [ – ]. I solu le de t a ould e applied as at i fo i o ilizatio of io ole ules [ ].
EXPERIMENTS
Isolatio a d s ree i g for dextra produ i g LAB
No - o e ial ate kefi g ai s f o a house-hold i Belg ade, “e ia e e used fo isolatio of LAB. The g ai s g e e ho oge ized i L sali e a d used as i o ulu fo t o diffe e t edia: MR“ a d M aga . Afte t o da s of i u atio at °C, fi e to
te olo ies f o ea h aga plate e e i o ulated i MR“ oth. The e e e-st eaked a d pu ified st ai s e e outi el p opagated i MR“ oth at °C i
i o-ae ofili o ditio fo fu the ha a te izatio . The fi st s ee i g fo de t a p odu tio as pe fo ed o odified MR“ MR“ aga ha i g % su -ose i stead of glu -ose. “ele ted ultu es e e st eaked o su fa e of aga plates. Aga edia e e i u ated i i o-ae ofili o ditio s at °C fo h. The sli
olo ies e e ide tified a d p opagated i MR“ oth. The a ou t of p odu ed de t a as esti ated olo i et i all phe ol–sulfu i a id ethod usi g glu ose as a sta da d [ ]. The a so a e as ea-su ed at i spe t ophoto ete Ult ospe p oA e sha Bios ie e .
Ide tifi atio of dextra -produ i g isolates
The th ee sele ted st ai s T , T a d T e e ide tified phe ot pi a d ole ula ethods. The fi st te tati e ide tifi atio as LAB as pe fo ed a o di g to o phologi al ha a te isti s olo o -pholog , G a stai i g a d ells shape a d ph sio-logi al ha a te isti s atalase test, g o th at diffe e t te pe atu es, g o th i edia ith diffe e t o e -t a-tio of NaCl . Fu -the , isola-tes e e e a i ed fo fe e tatio of diffe e t a oh d ate usi g the API
CHL s ste BioMé ieu , F a e .
The spe ies le el ide tifi atio of sele ted LAB de t a p odu i g st ai s as o fi ed se ue
-i g of the “ RNA e od-i g ge e, us-i g the fol-lo i g p o edu e: the total DNA e t a tio f o pu e
ultu es as do e phe ol- hlo ofo ethod [ ]. The “ RNA ge e as a plified usi g the total DNA as te plate fo PCR ith p i e s UNI “F ′-GAG AGT TTG ATC CTG GC- a d UNI “R ′-AGG AGG TGA TCC AGC CG- ′ . PCR i tu e o tai ed . μL PCR uf-fe . M KCl, . M T is-HCl, pH . at °C a d . % No idet P , . μL MgCl M , . μLult apu e dou le distilled H O, . μLTa pol e ase Fe -e tas, Lithua ia a d μL g-e o i DNA of th-e iso-late. The ea tio s e e a ied out i Ge eA p PCR C le Applied Bios ste s, Foste Cit , CA, U“A p og a ed as follo s: the i itial de atu atio of DNA fo i at °C, les of s at °C, s at °C, a d s at °C; a d the e te sio of i o plete
p odu ts fo i at °C. Resulti g PCR a pli o s
e e se ue ed at Ma oge i A ste da , the
Nethe la ds. The BLA“T algo ith http:// // . i. l . ih.go /BLA“T as used to dete i e the ost elated se ue e i the NCBI u leotide se ue e data ase.
Produ tio of dextra
Effe t of te pe atu e a d fe e tatio ti e o de t a ield
To e aluate the i flue e of te pe atu e a d i u atio ti e o the g o th a d de t a p odu tio , the th ee high ield de t a -p odu i g st ai s T , T a d T e e g o i L of MR“ oth o -tai i g g/L of su ose i L E le e e flasks at
°C fo h. This o e ight ultu e as used as
i o ulu i fu the e pe i e ts.
To stud the i flue e of a ious te pe atu es o de t a p odu tio the st ai s e e i u ated at , a d °C. I u atio lasted h fo L . ese
-t oides T , h fo L . ese -t oides T a d h fo L . hilga dii T .
To stud the effe t of ti e o de t a p odu tio , the ultu e edia e e i u ated fo diffe e t ti e i te als a gi g f o to h. Fe e tatio as a
-ied out at °C fo L . ese te oides st ai s T a d T
a d °C fo L . hilga dii T . De t a o e t atio ,
ell io ass a d fi al pH of fe e ted ultu e oth e e dete i ed.
Isolatio a d purifi atio of dextra
“a ples of the fe e ted edia fo de t a ua -tifi atio e e t eated i oili g ate fo i to i a ti ate a te ia. Ba te ial ells e e e o ed e t ifugatio at p fo i at °C. De t a
as p e ipitated f o the supe ata t additio of dou le olu e i e old % etha ol a d kept o e ight at – °C. The sa ples e e e t ifuged at p
fo i at °C. De t a pellets e e sepa ated f o
the supe ata t a d dissol ed i hot distilled ate . The p e ipitatio p o edu e as epeated t i e. The fi al p e ipitate as dissol ed i hot distilled ate . De t a sa ples e e additio all pu ified dial sis th ough kDa e a e agai st distilled ate at
°C fo h ith – ha ges pe da . The pu ified
de t a s e e the e o e ed sp a -d i g BÜCHI Mi i “p a D e B- , i let te pe atu e °C,
out-let te pe atu e °C a d sto ed at oo te pe
a-tu e.
Chara terizatio of dextra
Stud of o o e o positio
Baile a d O fo d [ ]. Glu ose, f u tose a d gala tose e e used as sta da ds.
Stud of Fou ie -t a sfo ed i f a ed spe t os op The ajo st u tu al g oups of the pu ified de t a s e e dete ted Fou ie -t a sfo ed i f a ed FTIR spe t os op usi g KB ethod.
FT-IR spe t os op Bo e MB , Ca ada as pe fo ed i i g the sa ple, de t a po de . g ith g of KB . The i tu e as ta leted u de a p essu e of MPa, a d d ied at °C fo
h efo e a al sis. The a al sis as pe fo ed at oo te pe atu e °C i the a ge of a ele gths
of – –, ith a esolutio of –.
RESULTS
Ide tifi atio of dextra -produ i g isolates
I the p ese t stud , la ti a id a te ia e e isolated f o ate kefi g ai s a d e e de oted as T to T . Isolated a te ia e e a le to g o o MR“ a d M plates at °C. All tested st ai s e e
at-alase- egati e a d G a -positi e ith a ious shapes a gi g f o lo g ods to o oid ods Ta le . Th ee isolates fo ed u ous olo ies o MR“ aga Fig. . The e e sele ted fo fu the stud i g a d de t a p odu tio ua tifi atio a d ha a te izatio .
Testi g the g o th at diffe e t te pe atu es sho ed that opti al te pe atu e fo all th ee st ai s as °C. The all ould g o at °C ut o e ould
g o at °C. The isolate T ould g o at °C, hile
T sho ed eak g o th at this te pe atu e. The iso-late T as tole a t to NaCl at all tested o e t atio s
, . a d . % , hile T a d T g e at a d . % NaCl a d did ot g o i the p ese e of . % NaCl. All th ee st ai s fe e ted glu ose ith gas p odu tio
Ta le .
Figu e . Pi tu e of isolates T left , T iddle a d T ight g o i g o the su fa e of the MRS aga ith % su ose plates.
API test as used fo dete i atio of fe e t-atio patte of isolates Ta le .
A o di g to ph siologi al ha a te isti s the iso-lates T a d T elo g to the ge us Leu o osto . The suga fe e tatio patte s diffe ed fo se e al a o-h d ates i ludi g a uti , f u tose, gala tose, la tose,
eli iose, sali i , t e halose, eth l-α-D-glu op a o-side, N-a et lglu osa i e, ge ti iose, i uli e a d tu a-ose Ta le . Based o the fe e tatio p ofile of the isolate T Ta le , this isolate as p eli i a il ide -tified as La to a illus hilga dii a o di g to Be ge ’s Ma ual of “ ste ati Ba te iolog [ ]. All th ee st ai s ould fe e t su ose hi h is the ai a oh d ate a aila le du i g ate kefi p epa atio .
Based o the “ RNA ge e e odi g se ue e of the isolates T a d T e e ide tified as Leu o osto ese te oides the isolates sha ed > % se ue e si ila it ith L . ese te oides st ai NCFB , a NR_ , hile the isolate T as ide tified as La -to a illus hilga dii ith % “ RNA e odi g se u-e u-e si ila it to st ai NBRC , a NR_ . . Based o the “ RNA ge e se ue es the isolates a d thei lose elati es, a ph loge eti t ee as o -st u ted the use of ARB soft a e [ ] Fig. .
Ta le . Mo phologi al a d ph siologi al ha a te isti s of the a te ial isolates; +: positi e ea tio , −: ega e ea o a d ±: eakl positi e ea tio
Cha a te isti Isolate
T T T
G a ea tio + + +
Cell fo Elo gated o i Elo gated o i Lo g ods
Cell size, μ . - . . - . . - .
Catalase – – –
G o th at diffe e t te pe atu es, °C – + ±
+ + +
+ + +
– – -
G o th i the p ese t of diffe e t
o e t atio s of NaCl, % . ± ± + ± ± ±
. – ± -
Dextra produ tio
The te pe atu e of i u atio sig ifi a tl i flu-e flu-ed thflu-e a ou t of p odu flu-ed dflu-e t a du i g g o th of all th ee isolates Fig. . The isolates e e g o o th ee te pe atu es of , a d °C. Fo L .
hil-ga dii T st ai , the a ou t of s thesized de t a a ied et ee the tested te pe atu es a d as the highest at °C, hile oth L . ese te oides st ai s
sho ed a i al de t a p odu tio at the lo est tested te pe atu e. The a i u de t a a ou t fo L . ese te oides T a d T st ai s as . a d . g/l, espe ti el . The e as a st o g de ease i de t a ield at highe te pe atu es. T st ai sho ed a i al de t a p odu tio of . g/L at the opti al g o th te pe atu e °C .
I this stud , de t a p odu tio L . ese -te oides T a d T st ai s a d L . hilga dii T st ai as
o ito ed du i g h i MR“ oth. The T a d T st ai s e e i u ated at °C a d T st ai as
i u ated at °C.The p odu tio of de t a i eased
ith ti e du i g the e po e tial g o th phase a d i
the statio a sta ted to de ease Fig. . De t a ield ea hed a i u afte h a d h of i u atio fo L . ese te oides T a d T st ai s, espe ti el . P o-lo ged ti e of fe e tatio had positi e effe t o de t a ield o l fo isolate T hi h ould e e p-lai ed slo e g o th ate of this a te iu . The
a i u de t a p odu tio fo the isolate T as o se ed afte h of fe e tatio , hi h as the egi i g of statio a phase of g o th of this a -te iu . The pH of the fe e -ted oth de eased f o the i itial pH of . to . du i g fe e tatio Fig. , due to the eta oli a ti it of isolates, p i a il la ti a id p odu tio . Co o fo all th ee st ai s as that he the pH alue d opped to . , the deg adatio of de t a sta ted, a d the ield as lo e .
Chara terizatio of dextra
TLC as pe fo ed usi g pape h o atog aph ith glu ose, f u tose a d gala tose as sta da ds. Results sho that glu ose is o o e u it of pol -Ta le . Ca oh d ates fe e tatio p ofiles of de t a -p odu i g LAB st ai s afte 8 h of i u atio at °C; +: positi e ea tio , −: ega e ea o a d ±: eakl positi e ea tio
Ca oh d ate
Ba te ia
Ca oh d ate
Ba te ia
L . ese te oides L . hilga dii L . ese te oides L . hilga dii
T T T T T T
Co t ol – – – Es uli + + +
Gl e ol – – – “ali i + – –
E th itol – – – Cello iose + + –
D-A a i ose – – – Maltose + + +
L-A a i ose – – + La tose + – –
Ri ose – + Meli iose + – –
D-X lose – + + “u ose + + +
L-X lose – – – T ehalose – + –
Ado itol – – – I uli – – –
eth l-β-D- lop a oside + – – Melezitose – – –
Gala tose + – – Raffi ose – – –
Glu ose + + + A ido – –
F u tose + + + Gl oge – – –
Ma ose – + – X litol – – –
“o ose – – – β-Ge ti iose – –
Rha ose – – – Tu a ose – + –
Dul itol – – – L ose – – –
I ositol – – – Tagatose – – –
Ma itol – – D-Fu ose – – –
“o itol – – – L-Fu ose – – –
eth l-α-D- a op a oside – – – D-A a itol – – –
eth l-α-D-glu op a oside – + – L-A a itol – – –
N-A et lglu osa i e – + – Glu o ate – +
A gdali + – – -Ketoglu o ate – – –
sa ha ides p odu ed all th ee studied isolates T , T a d T .
FTIR spe t os op a al sis o fi ed pol sa ha -ide atu e of the e t a ellula ate ial s thesized the tested isolates Fig. .
All sa ples a al zed o FTIR had si ila appea -a e i the -a e-a of – –. I te se a d oad
a d i – – o igi ated f o the O–H
st et hi g i atio s due to the i flue e of h d oge o ds hi h is ha a te isti fo pol sa ha ides ole-ules [ ]. Peak a ou d – is ha a te isti fo
the C-H st et hi g i atio .
The e a e slight diffe e es i these spe t a i the a ge of – –. B oad sig al f o C–O–C a d
Figu e . The ph loge eti t ee of the LAB st ai s T , T a d T isolated f o ate kefi g ai a d othe e e s of La to a illus a d Leu o osto ge e a, o st u ted usi g the S RNA ge e se ue es. The t ee as o st u ted usi g the eigh o joi i g algo ith as i ple e ted i ARB soft a e. Es he i hia oli is the oot of the t ee.
C–O at – – i di ated the p ese e of the
ha a te isti o ds of a oh d ates [ ]. I this a ge the ost i te se peak fo all sa ples a e i the
– – a d – – hi h o fi ed
that the tested o pou ds a e pol sa ha ides [ ]. A ha a te isti a so ptio at – – i
di-ates α- o figu atio of o o e i u its. The a se e
of the sig al at – i di ates the a se e of
β- o figu atio [ ]. Based o the o se ed spe t a, it
as o luded that EP“s p odu ed all th ee a alzed isolates e e of the sa e atu e: ho opol -sa ha ides of glu ose i α- o figu atio , a d the EP“
as ide tified as de t a .
DISCUSSION
The p ese t stud des i es th ee e LAB st ai s isolated f o ate kefi g ai s that a e a le to p o-du e de t a . De t a p oo-du tio is a p e ale t featu e a o g LAB [ ]. De t a has u e ous appli atio s i food as ell as i o -food i dust ies [ – ], although a g eate o e ial use is li ited elati el s all ields [ ]. Most of LAB p odu e ho opol sa ha ides
i ua tities lo e tha g/L he g o i g u de o -ditio s that a e ot opti ized fo EP“ p odu tio [ ].
We ha e sele ted a o g LAB st ai s isolated f o ate kefi g ai ased o the sli olo fo -atio as pote tial de t a p odu e s .The p eli i a s ee i g fo de t a p odu tio sho ed that these th ee st ai s e e pote t de t a p odu e s ith ields a gi g f o . to . g/L. De t a p odu tio ields o tai ed ith ou isolates is si ila o e eedi g the a i al ields of othe epo ted de t a -p o-du i g a te ia [ – ]. I the stud of Va de Meule a d olleagues, the total of st ai s e e isolated f o e eal a d dai p odu ts, of hi h o l p odu ed glu a s ith ields i a ge . – . g/L [ ]. Maju de a d o o ke s epo ted de t a ield of g/L p odu ed L . ese te oides NRRL B- u de opti ized o ditio s [ ]. This ield is si ila ith ou st ai L . ese te oidesT , hile st ai L . ese -te oidesT p odu ed % o e de t a f o the sa e a ou t of su ose i the ediu . The st ai L . es-e tes-e oides-es T o es-e tes-ed % of su oses-e i to des-e t a ,
hi h is highe tha that epo ted “a at a d ol-leagues [ ] i the stud of L . ese te oides CMG
. . Qade a d o o ke s opti ized o ditio s fo Figu e . Effe t of i u atio ti e o de t a ield, fi al pH a d ell g o th fo isolates T a , T a d T g o i MRS
de t a p odu tio L . ese te oides PC“IR- a d a hie ed . % o e sio [ ].
We dete i ed the opti al te pe atu e a d i u -atio ti e fo de t a p odu tio fo the th ee a al-zed st ai s. The opti al de t a p odu tio fo L . hilga dii T as a hie ed at °C, hi h is i ag
ee-e t ith p ee-e iousl pu lishee-ed data [ ]. L . hilga dii is espo si le fo de t a p odu tio a d g ai fo atio [ ]. Glu ose ole ules i this de t a a e li ked alpha - a d alpha - gl osidi o ds i the ai hai ith additio al alpha - o ds i the side hai . The p ese e of alpha - gl osidi o ds i de t a s is k o to e de the i solu le i ate [ ].
The opti al te pe atu e fo de t a p odu tio Leu o osto st ai s is st ai -depe de t a d a ies f o – °C [ , , ]. Both Leu o osto ese te oides
st ai s p ese ted i this pape p odu ed the highest a ou t of de t a at °C.
“i e o ditio s fo de t a p odu tio a e spe ifi fo ea h st ai , the ould e fu the opti ized. “e
-e al fa to s i ludi g su os-e o -e t atio , it og-e sou e, i e al salts, i itial pH ediu a d ae atio a i flue e de t a p odu tio [ , , , ]. Fu the opti izatio of o ditio s that fa o de t a p odu -tio fo ou st ai s has a pote tial to a hie e ields highe tha e e epo ted i the lite atu e, gi e that u de tested o ditio s, the de t a ields e e at le el of e eedi g those epo ted othe s. Also, the p odu t itself should e o e detailed ha a te ized. Fi st, ole ula ass should e dete i ed, as ell as fa to s affe ti g p odu tio of de t a desi ed ass,
hi h is i po ta t fo its appli atio .
CONCLUSION
Ou esults sho that ate kefi g ai s a e i h atu al sou e of pote t de t a -p odu i g LAB. Ou atte pt to isolate de t a p odu i g LAB f o a o
-o e ial ate kefi g ai s ge e ated th ee st ai s, hi h ields of de t a p odu tio a e e eedi g those Figu e . FT-IR spe t a of the e opol sa ha ides p odu ed the isolates L . ese te oides T , L . ese te oides T
epo ted i the lite atu e, e ause of hi h these st ai s a e o side ed as a didates fo o e ial e ploitatio .
A k o ledge e ts
The fi a ial suppo t fo this i estigatio gi e Mi ist of Edu atio , “ ie e a d Te h ologi al De elop e t of the Repu li of “e ia u de the p o-je t TR is g atefull a k o ledged.
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I)VOD
)RNO VODENOG KEFIRA KAO I)VOR BAKTERIJA MLEČNO KISELINSKOG VRENJA POTENTNIH PRODUCENATA DEKSTRANA
“lađa a ). Da ido ić , Mio a G. Miljko ić , Duša G. A to o ić , Mi ja a D. Rajilić-“toja o ić , “uza a I. Di it ije ić-B a ko ić
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U i e zitet u Beog adu, Teh ološko– etalu ški fakultet, Kated a za o ga sku he iju, Ka egije a , Beog ad, S ija
Nauč i ad
Vode i kefi je apitak koji astaje fe e ta io o akti ošću akte ija leč o-kiseli skog e ja, k asa a i si ćet ih akte ija. O a ko pleks a s eša ik oo ga iza a s ešte a je u uta polisaha id og at iksa – kefi og z a. Gla u šeće u ko po e tu at iksa či i dekst a koji si tetišu akte ije
leč o-kiseli skog e ja. Dekst a je ho o polisaha id elike olekulske ase čija je o o e a jedi i a glukoza. )ah aljujući do oj ast o lji osti i sta il osti, dekst a i a ši oku p i e u u azličiti g a a a i dust ije, kao što su edi i a, fa a ija, p eh a e a, tekstil a i he ijska i dust ija. Ne ast o i dekst a
ože da posluži kao osač za i o iliza iju io olekula. I ajući u idu eliku p i-e ji ost di-ekst a a, ilj o og ada ji-e io izolo ati akti-e iji-e li-eč o-kisi-eli skog e ja iz z a ode og kefi a koje p odukuju o aj egzopolisaha id sa isoki p i-osi a. U adu je p ikaza a izola ija i ka akte iza ija t i selekto a a soja i opti-iza ija uslo a za p oduk iju dekst a a. Na os o u o foloških, fizioloških i io-he ijskih oso i a i “ RNK sek e i a ja ut đe o je da su izolati T i T soje i ste Leu o osto ese te oides, dok je izolat T ide tifiko a kao La to a illus hilga dii. )a p oduk iju dekst a a ko išće a je odifiko a a MR“ podloga sa % saha oze, kao jedi i iz o o uglje ika. Maksi al i p i osi dekst a a , , , i , g/l su do ije i ako , i h fe e ta ije, za T , T i T , edo . Opti al e te pe atu e za p oduk iju dekst a a su °C za d a Leu
o-osto ese te oides soja, a za La to a illus hilga dii °C. Uzo i si tetisa ih polisaha ida su ide tifiko a i kao dekst a i a os o u papi e h o atog afije, dok su gla e st uktu e ka akte istike p ečišće ih dekst a a ut đe e a os o u FTIR spekt oskopije.
