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Human Cells Require Non-stop Ribosome Rescue Activity in Mitochondria.

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Academic year: 2017

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Fig 1. ICT1 and ArfB share conserved residues that are required for release activity. Clustal Omega alignment of human ICT1 and ArfB proteins from E.
Fig 2. ICT1 and ArfB hydrolyze peptidyl-tRNA on ribosomes near the 3 ’ end of an mRNA
Fig 3. ICT1 ribosome release activity supports viability in c . crescentus . A co-transduction experiment was used to test whether ICT1 complements the synthetic lethal phenotype of deleting ARFB and SSRA
Fig 4. expression of ICT1 partially complements the growth defect of δssra cells. Growth of wild-type cells, Δ SSRA cells, or Δ SSRA cells expressing ArfB or ICT1 from a plasmid was monitored during exponential phase
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