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Designed Amino Acid Feed in Improvement of Production and Quality Targets of a Therapeutic Monoclonal Antibody.

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Table 1. Plackett–Burmandesign matrix, twenty runs were designed at various combinations of ‘high’ (1) and ‘low’ (-1) values of 19 different amino acids.
Table 2. Box-Bencken design matrix, the low level used in the design (–1) was the same as the higher level used in the Plackett-Burman design
Table 5 summarizes the ANOVA results for the amino acids which were chosen based on the contribution% for the final mAb titre
Table 5. ANOVA results for Plackett-Burman analysis for the final mAb titre response
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