Ciência Rural, v.50, n.3, 2020.
Testers in supersweet corn lines
Avaliação de testadores em linhagens de milho superdoce Thiago Hideyo Nihei1 Filipe Augusto Bengosi Bertagna2* Maurício Carlos Kuki2
Carlos Alberto Scapim3 Ronald José Barth Pinto3 Antônio Teixeira do Amaral Júnior4
ISSNe 1678-4596
Received 06.13.19 Approved 11.22.19 Returned by the author 02.10.20 CR-2019-0447.R1
INTRODUCTION
Sweet corn is classified as a widely consumed vegetable, with a harvest between 20 and 25 days after pollination and high post-harvest decay. The ears can be used for fresh consumption in the form of sweet corn or by canning industries, as canned, frozen, or dried corn and in the form of baby or young corn (OLIVEIRA JUNIOR et al., 2006). The sugar content, flavor, pericarp texture and thickness, duration of the harvest period and
post-harvest durability of grain sweetness are some of the traits that make sweet corn more attractive for fresh consumption than field corn (BARBIERI et al., 2005).
The particularities of the most commonly used maize types allow a separation in two groups: “sweet”, which contains the sugary gene and “supersweet”, which carries the genes brittle (bt),
shrunken (sh) or sugary enhancer (se). Corns
carrying the sh allele have higher sucrose contents, the sugar with highest sweetening power, aside from
1Programa de Pós-graduação em Agronomia (PGA), Universidade Estadual de Maringá (UEM), Maringá, PR, Brasil.
2Programa de Pós-graduação em Genética e Melhoramento (PGM), Universidade Estadual de Maringá (UEM), 87020-900, Maringá, PR, Brasil. E-mail: filipeabbertagna@outlook.com. *Corresponding author.
3Departamento de Agronomia, Universidade Estadual de Maringá (UEM), Maringá, PR, Brasil.
4Departamento de Agronomia, Universidade Estadual Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brasil.
ABSTRACT: The purpose of this study was to evaluate the discriminatory capacity of tester lines for tropical corn lines converted to
supersweet shrunken (sh2) gene, for the development of hybrids adapted to tropical conditions. Lines were used as female parents in crosses with three testers: open-pollinated mixed variety; supersweet line L4; supersweet commercial hybrid Tropical Plus. Four trials were carried out to evaluate topcrosses in Maringá – PR e Sabáudia – PR, Brazil in the main growing season of 2015/16. The following traits were evaluated: total ear weight (TEW, in kg), commercial ear weight (CEW, in kg) and total soluble solids (TSS, in °Brix). The GCA estimates for TEW and CEW were highest for L4. The lines Balu-114 and UEM-25 were selected based on the effects of for the traits studied and should be used in the establishment of base populations for the breeding of superior lines. The value for TEW was highest for cross BALU-182 x Tropical, while for CEW was the highest value for cross BALU-94 x Mista.
Key words: diallel analysis, general and specific combining ability topcross, sweet corn, tester.
RESUMO: O presente trabalho teve como objetivo avaliar o desempenho de testadores quanto à capacidade de discriminação de linhagens
tropicais convertidas à superdoce, por meio da incorporação do gene shrunken (sh2), visando a produção de híbridos adaptados às condições tropicais. As linhagens utilizadas como parentais femininos foram os testadores: variedade de polinização aberta Mista; linhagem superdoce L4; híbrido comercial superdoce Tropical Plus. Os quatro experimentos foram conduzidos em Maringá – PR e Sabáudia - PR, na safra verão de 2015/16. As características avaliadas foram: Peso de espigas totais (PET, em kg), Peso de espigas comerciais (PEC, em kg) e sólidos solúveis totais (SST, em ºBrix). A linhagem L4 foi o testador que mais proporcionou efeito de heterose. As maiores estimativas de CGC para PET e PEC foram obtidas por L4. As linhagens Balu-114 e UEM-25 foram selecionados com base nos efeitos de para as características estudadas e deverão ser utilizados na formação de populações base para a extração de linhagens superiores. O cruzamento BALU-182 x Tropical apresentou o maior valor de para PET, enquanto o cruzamento BALU-94 x Mista obteve o melhor valor para PEC.
Palavras-chave: análise dialélica, capacidades geral e específica de combinação, topcross, milho doce, testador.
Ciência Rural, v.50, n.3, 2020. the advantage of a longer harvest period and
post-harvest durability (TRACY, 2001).
As a major field corn producer, Brazil has a high potential for sweet corn production. The greatest challenge is the development of sweet corn cultivars adapted to the regional conditions and that meet the requirements imposed by the processing industry. Compared to field corn, the number of sweet corn varieties available in germplasm banks is small. There are only about 300 open-pollinated sweet corn varieties in the world (TEIXEIRA et al., 2013). Brazil has only 58 registered sweet corn cultivars, few of which are available to farmers.
The narrow genetic basis of sweet corn may, in the near future, limit the advances in breeding programs and the development of new cultivars. Considering it, breeding programs seek methods that can be applied in sweet corn breeding. One of the most commonly used methods is the introduction of a gene that confers the trait sweet, known to be monogenic and recessive, of a random genetic source in a commercially used genotype with a normal endosperm, by successive backcrosses until the recovery of the desired genotype, maintaining the expression of the sweet gene (TRACY, 2001; BORDALLO et al., 2005).
Diallel analysis is one of the best methods of generating genetic information in breeding programs. This mating system allows the estimation of the general combining ability (GCA) and specific combining ability (SCA), which are associated with additive and non-additive gene effects, respectively (GRIFFING, 1956).The topcross method, among the possible evaluation methods of progenies, allowed the evaluation of the combining ability of a large number of lines, at different inbreeding levels, with a common tester. Thus, this method allowed the selection of the best lines, increasing the efficiency and making the development of hybrids more practical (MIRANDA FILHO & VIÉGAS, 1987).
Maximization of heterotic effects should be considered in any corn breeding program, since parent grouping according to the heterotic pattern is useful in the definition of promising crosses. However, which parents would be indicated as contrasting patterns for sweet corn is still undefined. In Brazil, there are no reports of the use of the top-cross technique for sweet corn lines.
The purpose of this study was to evaluate the performance of testers and tropical corn lines converted to supersweet by the introgression of the sh gene with regard to the general and specific combining ability, with a view to selecting superior lines for hybrid development in sweet corn breeding.
MATERIALS AND METHODS
The study was divided in two stages: in the first, 48 field maize lines were selected for the introduction of the recessive supersweet sh gene by backcrossing. The donor of gene sh was the single-cross hybrid Tropical Plus. Backcrossing was continued until the recovery of 93.75% of the genes from the field maize lines. Thereafter, the lines were used as female parents in crosses with three testers: a) open-pollinated mixed variety (broad genetic base tester); b) supersweet line L4 (narrow genetic base tester); c) supersweet commercial single-cross hybrid Tropical Plus (narrow genetic base tester).
The second step consisted in the evaluation of topcross hybrids and lines per se. Three experiments were conducted to evaluate topcrosses derived from the cross of lines with each tester, and one to evaluate the per se performance of the lines. The four trials were carried out in the summer growing season of 2015/16 at two locations: on the Experimental Farm of Iguatemi (23º25’S, 51º57’O, 550 m asl), in Maringá, Paraná, and at Sementes Balu farm (23º19’S; 51º33’; 725m asl), in Sabáudia, Paraná. The trial was arranged in a complete block design with randomized treatments, with two replications. The plot consisted of one 5-m row, with rows spaced 0.70 m and plants 0.2 m apart, totaling 71,000 plants ha1.
The milky grain stage (R3) was defined as standard for the harvest of sweet corn, with a grain moisture content between 70 and 80%. The following traits were evaluated: total ear weight (TEW, in kg), i.e., the mean of the total weight of husked ears per treatment; commercial ear weight (CEW, in kg), i.e.; the mean weight per treatment of the ears considered commercial (length > 15 cm, diameter > 3 cm, free of insect and disease damage); total soluble solids (TSS, in °Brix).
A partial diallel analysis was performed by the model proposed by GRIFFING (1956), adapted for partial diallel crosses by Geraldi and Miranda Filho (1988), based on the treatment means of each trial. The variance components and genetic and phenotypic parameters were estimated, e.g., estimates of genetic variance , phenotypic variance , heritability (h2), coefficient of genetic variation , and the
ratio of the per se lines and topcross hybrids. For the statistical and genetic analysis, software Genes was used (CRUZ, 2013).
RESULTS AND DISCUSSION
The coefficients of variation and the genetic parameters were illustrated at table1. The CVg indicates
Ciência Rural, v.50, n.3, 2020. the proportional selection gain in relation to the mean,
and a ratio of <1 indicates a favorable situation for selection (RODRIGUES et al., 2009).
In this study, the values of this ratio were highest in the combined analysis, i.e., 1.17, 1.02 and 0.44 for the topcrosses involving the testers L4, Tropical and Mixed variety, respectively, indicating the possibility of selection for the traits evaluated at both locations for each tester. In addition, this result showed that a major part of the variation was due to genetic variance, favoring genetic gains in relation to the original mean.
The genetic parameters indicated tester Tropical as the most efficient for TEW compared
with the other two testers, since the indices of the topcrosses of the lines with this tester were good (h2 of 80.97 and CVg/CVe 1.08). However, despite the apparent superiority of tester Tropical, the Mixed variety was more efficient in discriminating lines with regard to TSS; and although, the topcross means involving Tropical were higher for both TEW and CEW, the Mixed variety proved to be the best tester for the differentiation of lines for commercial ear yield. The low CVg and heritability values (Table 1) for the per se performance of the lines indicated that genetic gains with selection may be hampered. These values suggested a separate analysis of these lines in
Table 1 - Estimates of genetic and phenotypic parameters for total ear weight (TEW), commercial ear weight (CEW) and total soluble solids (TSS) of hybrids. Growing season 2015/2016 - Sabáudia (SAB) and Maringá-PR (MGA).
Estimators ---TEW (kg)--- ---CEW (kg)--- ---TSS (%)---
SAB MGA SAB MGA SAB MGA
---Tester 1 (Mixed)--- 2 g
σ
0.20 0.22 0.38 0.26 - - 2 fσ
0.43 0.30 0.68 0.37 0.45 0.62 2 h 45.12 71.257 56.26 69.58 - - g CV 7.80 11.15 11.80 12.60 - - / g e CV CV 0.64 1.11 0.80 1.07 - - ---Tester 2 (L.4)--- 2 gσ
0.95 0.20 1.37 0.18 0.45 - 2 f σ 1.05 0.31 1.49 0.33 0.63 0.32 2 h 90.79 62.34 91.65 24.983 71.33 - g CV 15.05 10.06 19.837 10.03 4.23 - / g e CV CV 2.22 0.91 2.34 0.79 1.12 - ---Tester 3 (Tropical)--- 2 g σ 0.38 0.14 0.56 0.17 0.35 - 2 fσ
0.52 0.22 0.75 0.26 0.64 0.56 2 h 73.54 64.62 75.06 63.72 55.29 - g CV 10.86 8.66 14.20 9.55 3.74 - / g e CV CV 1.18 0.96 1.23 0.94 0.79 ----Per se line performance--- 2 g
σ
0.38 0.27 0.46 0.43 2.17 2.48 2 f σ 0.40 0.28 0.47 0.43 4.82 3.32 2 h 96.76 98.00 97.98 99.67 45.13 74.87 g CV 20.75 34.045 37.14 207.59 8.93 10.22 / g e CV CV 3.86 4.95 4.92 12.32 0.64 1.22Ciência Rural, v.50, n.3, 2020. each environment, to ensure more consistent indices
for the evaluated traits.
Results of the combined analysis of variance were statistically significant (p<0.05) for the interaction topcrosses and environments. This allowed the conclusion that the performances differed between the two environments, indicating that in the mean, some genotypes had a differentiated relative performance due to environmental variations, which can be attributed to genetic differences between the respective lines of hybrid topcrosses, requiring an interpretation based on the treatment means in each tested environment.
The summary of the combined diallel analysis for the response variables TEW, CEW and TSS is shown in table 2. Results of the mean squares of the combined diallel analysis showed that the effects of the general combining ability of the topcrosses (GCAI), lines (GCAII) and also for the specific combining ability (SCA) were highly significant (p<0.05) for all evaluated traits.
Significant mean square values for GCA indicated the presence of at least one genotype among the testers (group I) and another among the lines (group II) that differs from the others in the concentration of favorable alleles for the trait; independently, of the dominance type of these alleles (VENCOVSKY & BARRIGA, 1992). Thus, results obtained in this diallel analysis indicated the presence of additive and non-additive effects on the genotypic variance of the traits; although, the values of the quadratic components showed that the additive effects were more important than the non-additive effects on these traits.
Results for the interaction of GCAI and GCAII with environments (Table 2) showed a variable performance of lines and testers regarding their additive effects according to each environment. Similarly, the SCA - environment interaction indicated that it is important to define the best crosses in terms of the individual combining ability in each location.
The GCA estimates for the testers (GCAI) and lines (GCAII) are listed in tables 3 and 4. The highest CA estimates for TEW and CEW were obtained by the narrow genetic base tester L4. These results demonstrated that tester L4 has the highest frequency of favorable alleles to raise the trait means, and the tester of broad genetic base Mixed variety has the lowest. Moreover, the high effects reflect the high degree of homozygosity and the narrow genetic base of line L4, aside from a good GCA with the lines converted to the trait sweet.
An optimal tester maximizes line performance and efficiently discriminates the tested lines. Although, an efficient tester can be defined as one that can generate different hybrids with itself, without strongly influencing the average performance of these hybrids. Therefore, the best tester would be a homozygous recessive line or a population with low allele frequency for the most important loci. This type of tester would be most adequate for the assessment of lines in the initial stages of selfing, from topcross mating systems (MIRANDA FILHO & VIÉGAS, 1987).
General combining values for the Mixed variety were negative for the three variables, which may qualify this tester as an excellent option for selection of lines with good GCA, since their negative
Table 2 - Combined partial diallel analysis for total ear weight (TEW), commercial ear weight (CEW) and total soluble solids (TSS) of topcross hybrids. Growing season 2015/2016 - Sabáudia and Maringá-PR.
Sources of variation DF ---Mean squares---
TEW (kg) CEW (kg) TSS (%) Topcrosses 143 1.3797** 1.7383** 1.3886** GCA(testers) 2 11.7858** 12.0320** 11.6452** GCA(lines) 47 1.5090** 2.4939** 2.0175** SCA 94 1.0936** 1.1415** 0.8559** Environments 1 358.0137** 240.5937** 414.1225** Topcrosses× environments 143 0.6199** 0.9351** 0.8880**
GCA (testers) × environments 2 5.5124** 4.8069** 0.1590*
GCA (lines) × environments 47 0.5659** 1.4158** 1.3020**
SCA × environments 94 0.5428** 0.6123** 0.6965*
Combined error 288 0.25250 0.32150 0.71100
Ciência Rural, v.50, n.3, 2020. values would facilitate the expression of good GCA of
the evaluated lines. However, tester L4 proved efficient for the selection of lines to achieve genetic gains for TEW and CEW, since the GCA estimates for these traits were positive. This observation is justified by the fact that, when using an inbreeding line as tester, the main objective was to select lines for SCA, especially when the tester has relevant GCA estimates.
Tropical tester obtained negative or irrelevant estimates for TEW and CEW (Table 3), which results in an efficient discrimination of genotypes for these traits. The negative values of tester Tropical may be a consequence of its genetic correlation with the lines, for being used as donor of the gene supersweet. Therefore, the topcrosses involving these genotypes could be interpreted as intrapopulational crosses (HALLAUER & MIRANDA FILHO, 2010).
The results for the GCA effects of lines (GCAII) are shown in table 4. Generally, a line has a higher or lower effect than the others when the difference between the values is greater than twice the value of the calculated standard deviation. If the positive of estimates meet this requirement, the lines under study are expected to induce an improvement in the topcross hybrids (WERLEET al., 2014). According to CRUZ and REGAZZI (2001), high positive or negative values, respectively, showed that the parent in question is superior or inferior to the other parents of diallel, in terms of average progeny performance.
Considering the results for the GCAII estimates for trait TEW, seven lines performed better in Sabáudia and six in Maringá, with particular emphasis for line UEM-23, with highest effects at both locations. Eight lines were considered superior for CEW; however, there was a consistency between these lines as the evaluation sites. For TSS, nine lines had higher GCA estimates; although, none of them in
both environments. Therefore, it is possible to state that the absence of a line with a higher effect at both locations, for CEW and TSS, is related to a significant GCAII – environment interaction (Table 3), requiring selection of the best lines within each environment for the formation of topcross hybrids.
Considering the traits TEW and CEW, lineUEM-23 had a particularly good performance, with constant positive and significant estimates at both locations. For the same traits, there were two groups of lines that only had great values either in Sabáudia (Balu-28, Balu-29, Balu-30, 9, UEM-11, UEM-23, and UEM-25) or in Maringá (Balu-18, Balu-44, Balu-138, and UEM-23).
The SCA reflects the specificity between the parents involved in crosses, being explained by the effect of complementation between the alleles derived from each parent (dominant gene action) and the effect of interactions between the alleles of different loci involved in the trait transmission (epistatic gene action). High values, regardless of the signal, indicated that the SCA performance were different than the expected based on the GCA of the parents. Although, for selecting the best crosses based on the SCA effects, at least one parent with high GCA should be present at the cross (CRUZ et al., 2012). Furthermore, SCA is also related with the genetic distance between the parents and demonstrated the importance of non additive interactions to complement the performance of the hybrid combination.
Considering SCA results, the cross Balu182 x Tropical had positive effects for TEW in Sabáudia and CEW in Sabáudia and Maringá. For the same trait, the crosses Balu 19 x Tropical, Balu 25 x Tropical, Balu 114 x Tropical, Balu 52 x Mista, Balu 94 x Mista, and UEM 19 x line L4 also had a good specific performance for both traits. Besides the SCA
Table 3 - General combining ability estimates (𝑔𝑔�i) of the testers for total ear weight (TEW), commercial ear weight (CEW) and total soluble solids (TSS) of topcross hybrids. Growing season 2015/2016 - Sabáudia (SAB) and Maringá-PR (MGA).
---Mean squares--- ---TEW (kg)--- ---CEW (kg)--- ---TSS (%)---
SAB MGA SAB MGA SAB MGA
Mixed variety -0.224 -0.158 6.15 -0.178 -0.29 -0.28
L4 0.463 0.096 0.449 0.106 0.17 0.11
Tropical Plus 47 0.062 -0.208 0.072 0.12 0.17
SD(𝑔𝑔�i) 0.047 0.036 0.053 0.041 0.06 0.08
Ciência Rural, v.50, n.3, 2020.
Table 4 - Estimates of general combining ability (𝑔𝑔�i) of the lines for total ear weight (TEW), commercial ear weight (CEW) and total soluble solids (TSS). Growing season 2015/2016 - Sabáudia (SAB) and Maringá-PR (MGA).
𝑔𝑔�i effect ---TEW (kg)--- ---CEW (kg)--- ---TSS (%)---
SAB MGA SAB MGA SAB MGA
Balu-3 122.5 -0.987 0 37.895 1.07 0.27 Balu-7 0.042 0.220 0.079 0.267 -0.13 0.17 Balu-8 -0.086 0.204 -0.024 0.284 -0.23 0.51 Balu-9 0.029 0.122 0.103 0.178 0.37 0.01 Balu-18 0.034 0.390 0.148 0.427 0.17 -0.26 Balu-20 24.983 0.253 -0.635 0.252 -0.23 -0.59 Balu-21 -0.654 51.612 9.4 -0.085 -0.27 -0.09 Balu-22 24.983 -0.180 -0.323 -0.085 -0.43 0.04 Balu-28 0.876 0.036 1.148 0.069 1.20 0.07 Balu-29 0.899 0.088 1.064 0.140 0.17 0.04 Balu-30 0.567 0.026 0.731 0.117 -0.13 1.21 Balu-40 -0.079 -0.069 -0.278 0.124 0.10 0.84 Balu-44 0.110 0.416 0.084 0.418 -0.37 0.11 Balu-48 -0.553 0.164 -0.508 0.172 -0.87 -0.06 Balu-52 0.147 -0.058 47 -0.112 0.50 -0.03 Balu-55 -0.103 0.147 0.090 0.164 0.54 0.14 Balu-56 0.220 -0.135 0.225 -0.199 0.20 0.24 Balu-58 -0.043 -0.142 0.076 -0.070 -0.17 -0.03 Balu-72 -0.011 0.265 0.283 0.288 -0.13 -0.03 Balu-79 -0.072 0.381 -0.103 0.303 24.983 -0.26 Balu-80 51.612 0.237 -0.050 0.290 -1.10 -0.76 Balu-83 -0.113 0.069 0.097 0.125 -0.40 0.31 Balu-89 -0.605 -0.359 9.4 -0.248 0.04 -0.33 Balu-90 -0.854 -0.192 -0.652 18.25 0.07 -0.69 Balu-94 -0.086 -0.179 0.096 -0.279 0.14 -0.09 Balu-95 -0.147 -0.486 -0.211 6.15 0.04 0.77 Balu-111 -0.022 -0.037 0.102 0.007 0.67 0.34 Balu-114 0.184 -0.066 0.335 -0.010 0.77 -0.39 Balu-115 0.158 -0.166 0.182 -0.156 -0.13 0.27 Balu-116 -0.087 -0.153 19.5 -0.134 -0.33 0.07 Balu-138 0.373 0.676 0.548 0.765 0.47 0.61 Balu-143 0.209 -0.487 0.446 51.612 -0.17 -0.26 Balu-148 -0.011 -0.434 0.133 -0.388 -1.17 -0.16 Balu-169 -0.081 0.323 0.176 0.379 -0.17 -1.59 Balu-181 0.051 -0.104 0.437 76 0.24 0.24 Balu-182 -1.127 -0.488 -1.030 -0.473 -0.63 0 Balu-183 -0.168 -0.090 0.014 -0.200 -0.80 0.41 Balu-185 -0.961 -0.584 -0.790 -0.637 -0.30 0.31 UEM-3 -122.291 -0.497 -0.029 -0.537 0.07 0.87 UEM-6 -0.269 -0.060 79 12.5 0.67 0.21 UEM-9 0.731 0.128 0.806 -0.049 0.17 -0.99 UEM-10 0.434 0.407 0.457 0.378 0.24 0.21 UEM-11 0.720 0.282 0.729 0.228 0.50 0.11 UEM-16 0.306 0.010 0.157 0.014 0.54 0.37 UEM-19 0.380 0.244 0.490 0.175 -0.13 -0.73 UEM-22 0.114 0.334 76 0.341 -0.17 -0.59 UEM-23 0.604 0.598 0.707 0.584 0.57 -0.13 UEM-25 1.094 0.004 1.028 -0.109 0.37 0.21 SD(𝑔𝑔�i) 0.227 0.176 0.257 0.197 0.292 0.383 SD(𝑔𝑔�i-𝑔𝑔�i') 0.324 0.252 0.368 0.281 0.417 0.548 2x SD(𝑔𝑔�i) 0.454 0.352 0.514 0.394 0.584 0.766
Ciência Rural, v.50, n.3, 2020. significance effect for TSS, the values obtained in
this study were not promising for enhancing this trait. The cross Balu-182 x Tropical showed the highest value for TEW (0.711) and CEW (1.270) in Sabáudia. Likewise, the crossBalu-94 x Mixed variety had the highest value (0.750) for CEW in Maringá. Thus, these lines can be used as testers of lines derived from the respective testers. Therefore, the development of new lines derived from each one of these testers that can provide high GCA estimates is recommended.
In diallel analyses, only SCA estimates originated from crosses involving at least one parent with high GCA should be considered. In this way, analyzing GCA estimates simultaneously, only two inbred lines stand out among the highest values in both tables of estimates, Balu-114 and UEM-25. No cross had relevant estimates for both testers. This finding suggested that the testers have different genetic bases, with the ability to generate high heterosis in crosses with different groups of lines. This result allowed the conclusion that the testers used are originated from distinct heterotic groups.
CONCLUSION
Line L4 was the tester that provided highest heterotic effects in crosses with the lines. Highest GCA estimates for TEW and CEW were obtained by L4. The lines Balu-114 and UEM-25 were selected based on the effects for the traits studied and should be included in the formation of base populations for the breeding of superior lines. Cross BALU-182 x Tropical had the highest value for TEW and cross BALU-94 x Mista for CEW.
ACKNOWLEDGEMENTS
The authors thank to the e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the financial support.
DECLARATION OF CONFLICT OF INTERESTS
The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
AUTHORS’ CONTRIBUTIONS
All authors contributed equally for the conception and writing of the manuscript. All authors critically revised the manuscript and approved of the final version.
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